Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,329,852 C→A 5.2% P165T (CCT→ACT)  yjcZ → YjcZ family protein; yhjH motility defect suppressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,329,8520CA 5.2% 276.9 / 2.4 77P165T (CCT→ACT) yjcZYjcZ family protein; yhjH motility defect suppressor
Reads supporting (aligned to +/- strand):  ref base C (29/44);  new base A (2/2);  total (31/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAT  >  NC_000913/4329720‑4329991
                                                                                                                                    |                                                                                                                                           
gtgTGGTTTTCAGCCCGTTGTCGCTGGAAGGTTATGGCGTTAACGCAACGAGGCCTACTGGTGCTGGCAGGTGTTCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATTACGCCTGGTCGTTGCCTGGgcg                                                                                                                                      <  2:664467/138‑1 (MQ=255)
gAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGgcg                                                                                                                                      <  2:1673163/140‑1 (MQ=255)
gAGTGGTTGTCAGCCCGTTGTCGCTGGAAGGAATAGGCGTTACCGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTGTTACCCGCTGGCGTGCTGGGGCGTTTAACGCCTGGTCGTTGCCTGGgcg                                                                                                                                      <  2:663554/140‑1 (MQ=255)
gAGGGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGgcg                                                                                                                                      <  2:1673151/140‑1 (MQ=255)
       tttCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGtttt                                                                                                                               >  1:469329/1‑140 (MQ=255)
        ttCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTa                                                                                                                              >  1:453819/1‑140 (MQ=255)
        ttCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTa                                                                                                                              >  1:455608/1‑140 (MQ=255)
         tCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTAt                                                                                                                             <  2:740105/140‑1 (MQ=255)
           aGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCg                                                                                                                           <  1:1346607/140‑1 (MQ=255)
           aGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCg                                                                                                                           <  1:1157915/140‑1 (MQ=255)
                 ttGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTgg                                                                                                                     <  2:835837/140‑1 (MQ=255)
                 ttGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTgg                                                                                                                     <  2:584354/140‑1 (MQ=255)
                  tGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGACTGGGCGCTGTTTTATCGTTCTgga                                                                                                                    >  2:503322/1‑140 (MQ=255)
                  tGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGACTGGGCGCTGTTTTATCGTTCTgga                                                                                                                    >  2:503249/1‑140 (MQ=255)
                   gTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTggag                                                                                                                   <  2:862058/140‑1 (MQ=255)
                                aaaGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggg                                                                                                      <  2:1104259/140‑1 (MQ=255)
                                  aGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggg                                                                                                    <  1:1001596/140‑1 (MQ=255)
                                  aGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCATGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggg                                                                                                    <  1:1001627/140‑1 (MQ=255)
                                    gCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTAGCGCTGGGGGGc                                                                                                  >  1:1424711/1‑140 (MQ=255)
                                     cGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCa                                                                                                 <  2:1667360/140‑1 (MQ=255)
                                        tAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTGGATCGTTCTGGAGGAGTTGCGCTGGGGGGCAttt                                                                                              >  1:770191/1‑140 (MQ=255)
                                         aaCGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTg                                                                                             >  1:781065/1‑140 (MQ=255)
                                           cGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGc                                                                                           <  2:1829899/140‑1 (MQ=255)
                                           cGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGc                                                                                           <  2:633949/140‑1 (MQ=255)
                                              aaCGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGAt                                                                                        >  1:486319/1‑140 (MQ=255)
                                               aCGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGAtg                                                                                       >  1:1372029/1‑140 (MQ=255)
                                                        aCTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTc                                                                              >  2:1408188/1‑140 (MQ=255)
                                                               caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAg                                                                       >  2:806963/1‑140 (MQ=255)
                                                               caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATATGGCGATGCCTGCCGTCTGGGAAg                                                                       >  2:369501/1‑140 (MQ=255)
                                                               cacgcatgTGATCAGAGATAGCAAAAATTTAAACGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTCCCAGGTCGCTGTTTTATCCTTGAGGAGCAGTTGCGCTGGGGTGCATATGGCGATGACTGCCGACTGGGaat                                                                       >  2:1225827/4‑139 (MQ=255)
                                                                     ggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCa                                                                 <  1:406589/140‑1 (MQ=255)
                                                                       tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                               <  2:1551208/140‑1 (MQ=255)
                                                                       tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                               <  2:1551169/140‑1 (MQ=255)
                                                                       tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTGATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                               <  2:1551941/140‑1 (MQ=255)
                                                                              agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTTGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                        >  1:1224726/1‑140 (MQ=255)
                                                                              agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                        >  2:867633/1‑140 (MQ=255)
                                                                              agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                        >  1:721189/1‑140 (MQ=255)
                                                                              agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCATGGGCGCTGTGTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                        >  2:739579/1‑140 (MQ=255)
                                                                               gcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCg                                                       <  1:817350/140‑1 (MQ=255)
                                                                                cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                      >  1:904384/1‑140 (MQ=255)
                                                                                cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                      >  1:904684/1‑140 (MQ=255)
                                                                                cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                      >  1:467180/1‑140 (MQ=255)
                                                                                       aTTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCt                                               <  2:1056740/140‑1 (MQ=255)
                                                                                                  cTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGc                                    >  2:131693/1‑140 (MQ=255)
                                                                                                  cTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGc                                    >  2:131659/1‑140 (MQ=255)
                                                                                                         gCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGaa                             <  1:1382364/140‑1 (MQ=255)
                                                                                                           tGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                           <  2:1085956/140‑1 (MQ=255)
                                                                                                           tGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                           <  2:315460/140‑1 (MQ=255)
                                                                                                                cGTATAACGCCTGGTCGTTGCCTGGGCGCTGTGTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                      <  1:1321313/140‑1 (MQ=255)
                                                                                                                  tttaACGCCTGGTCGTTTCGTGGGCGCTGTTTTATCGTTGTGGTTGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCTAGCGGTGGCGTTGTTGCTGGGTGATTTTCACTAGAAAGCGAcaca                    <  2:451374/138‑1 (MQ=255)
                                                                                                                      aCGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACAt                <  1:1564133/140‑1 (MQ=255)
                                                                                                                      aCGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACAt                <  2:645842/140‑1 (MQ=255)
                                                                                                                      aCGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTGGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACAt                <  2:101819/140‑1 (MQ=255)
                                                                                                                      aCGCCTGGTCGTTGCCTGGGCGCAGTTTTAACGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACAt                <  1:1372109/140‑1 (MQ=255)
                                                                                                                         ccTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTg             >  2:1144844/1‑140 (MQ=255)
                                                                                                                         ccTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTg             >  2:1646216/1‑140 (MQ=255)
                                                                                                                          cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg            <  2:800219/140‑1 (MQ=255)
                                                                                                                          cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg            <  1:1891165/140‑1 (MQ=255)
                                                                                                                          cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg            <  1:1094896/140‑1 (MQ=255)
                                                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa          <  1:1697768/140‑1 (MQ=255)
                                                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa          <  1:1697555/140‑1 (MQ=255)
                                                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa          >  1:1226884/1‑140 (MQ=255)
                                                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa          <  2:486319/140‑1 (MQ=255)
                                                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa          <  1:949846/140‑1 (MQ=255)
                                                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa          <  1:84251/140‑1 (MQ=255)
                                                                                                                            ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCGGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa          <  2:272473/140‑1 (MQ=255)
                                                                                                                              tCGTTGCCTGGGCGCTGTTTTATCGTTCTGGTGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCtga        <  2:1860349/140‑1 (MQ=255)
                                                                                                                              tCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCaga        <  2:1257434/140‑1 (MQ=255)
                                                                                                                              tCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCaga        <  2:283951/140‑1 (MQ=255)
                                                                                                                              tCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCAGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCaga        <  2:1233455/140‑1 (MQ=255)
                                                                                                                              tCGTTGACTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCaga        <  1:503322/140‑1 (MQ=255)
                                                                                                                              tCGTTGACTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGAATTTGGCGATGCCTGCCGTCTGGTAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGTAAGCGACACAACATCTGGCaga        <  1:503249/140‑1 (MQ=255)
                                                                                                                                  tGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGt    >  2:669719/1‑140 (MQ=255)
                                                                                                                                  tGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGt    >  1:1643300/1‑140 (MQ=255)
                                                                                                                                  tGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGt    >  1:457849/1‑140 (MQ=255)
                                                                                                                                   gCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTa   >  1:1833548/1‑140 (MQ=255)
                                                                                                                                   gCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTa   >  1:1550668/1‑140 (MQ=255)
                                                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt  <  1:1403089/140‑1 (MQ=255)
                                                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt  <  1:1437574/140‑1 (MQ=255)
                                                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt  <  1:168816/140‑1 (MQ=255)
                                                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt  <  1:169388/140‑1 (MQ=255)
                                                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt  <  1:730148/140‑1 (MQ=255)
                                                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt  <  1:321892/140‑1 (MQ=255)
                                                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt  <  2:102270/140‑1 (MQ=255)
                                                                                                                                    ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt  <  1:730119/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                           
GAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAT  >  NC_000913/4329720‑4329991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: