Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,550,253	C→T	5.1%	V348V (GTG→GTA)	gntP ←	fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,550,253	0	C	T	5.1%	278.0 / 3.8	79	V348V (GTG→GTA)	gntP	fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base C (24/51);  new base T (2/2);  total (26/53)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCA  >  NC_000913/4550115‑4550391                                                                                                                                           |                                                                                                                                           cGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGca                                                                                                                                           >  2:1312030/1‑140 (MQ=255) cGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGca                                                                                                                                           <  1:1281658/140‑1 (MQ=255) cGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGca                                                                                                                                           >  1:493683/1‑140 (MQ=255) cGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGca                                                                                                                                           <  1:1833032/140‑1 (MQ=255) cGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGca                                                                                                                                           <  1:1634105/140‑1 (MQ=255) cGCAGCCGTCGCCAGTACCAGCAGCGCCGGAGTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGca                                                                                                                                           <  2:983657/140‑1 (MQ=255)   cAGCCGTCGCCAGTTCCAGCAGCGCCGGTTTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGcaca                                                                                                                                         <  2:368781/140‑1 (MQ=255)        gTCGCCAGTACCAGCAGCGCCGGTTTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGGGTTCATCGCCGAAACGACACCCTGACCCGTAGCCAGACGAATTAGCACAGTGAt                                                                                                                                    <  1:62118/140‑1 (MQ=255)        gTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGAt                                                                                                                                    <  2:1240464/140‑1 (MQ=255)        gTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGAt                                                                                                                                    <  1:1426971/140‑1 (MQ=255)        gTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGAt                                                                                                                                    <  1:715647/140‑1 (MQ=255)             cAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgcc                                                                                                                               <  1:754753/140‑1 (MQ=255)             cAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGAACAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgcc                                                                                                                               <  2:1392426/140‑1 (MQ=255)              aGTACCAGCTGCGCCGGTTTCACGCCAACCAGCTGACCTGTTGCTGGATCTTGGATTGCAGCTCTGATTATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgcca                                                                                                                              <  1:995604/140‑1 (MQ=255)              aGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgcca                                                                                                                              <  1:525313/140‑1 (MQ=255)              aGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgcca                                                                                                                              <  1:1470421/140‑1 (MQ=255)              aGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgcca                                                                                                                              <  1:1043690/140‑1 (MQ=255)                tACCAGCAGCGCCGGAGTCACGCCACCCAGCTGACCAGTTGCTGGATATAGGAGTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGTCCCGTCGCCAGACGAATTAGCACAGTGATCAgccatg                                                                                                                            <  2:174939/140‑1 (MQ=255)                 aCCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgc                                                                                                                           <  1:585734/140‑1 (MQ=255)                  ccAGCAGCGCCGGATTCACGCCAACCAGCTGACCTGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgcc                                                                                                                          <  1:234345/140‑1 (MQ=255)                  ccAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgcc                                                                                                                          <  1:258948/140‑1 (MQ=255)                  ccAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgcc                                                                                                                          <  2:1763002/140‑1 (MQ=255)                  ccAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgcc                                                                                                                          <  2:1763081/140‑1 (MQ=255)                  ccAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgcc                                                                                                                          <  2:1762894/140‑1 (MQ=255)                  ccAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgcc                                                                                                                          <  1:260451/140‑1 (MQ=255)                  ccAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgcc                                                                                                                          <  1:1133375/140‑1 (MQ=255)                     gcagcGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGccatgccatg                                                                                                                       >  1:964455/1‑140 (MQ=255)                       agcGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTCGGATTGCAGCACTGATAATCCCGGCGGCGGTCCTCGCCGACACGACACCCTGACCCGTCGCCAGACGACTTAGCACCGTGATCAGCCATGCCatgag                                                                                                                     >  2:1209726/1‑139 (MQ=255)                       agcGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCatgat                                                                                                                     >  2:772536/1‑140 (MQ=255)                       agcGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGACAGACGAATTAGCACAGTGATCAGCCATGCCatgat                                                                                                                     >  2:1381712/1‑140 (MQ=255)                        gcgcCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCatgatg                                                                                                                    <  1:1616124/140‑1 (MQ=255)                        gcgcCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCatgatg                                                                                                                    <  1:1513337/140‑1 (MQ=255)                           ccGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAg                                                                                                                 >  2:1798836/1‑140 (MQ=255)                             ggATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAggg                                                                                                               <  1:143793/140‑1 (MQ=255)                             ggATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAggg                                                                                                               <  2:1773619/140‑1 (MQ=255)                             ggATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAggg                                                                                                               <  1:143696/140‑1 (MQ=255)                             ggATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAggg                                                                                                               <  2:693102/140‑1 (MQ=255)                             ggATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGAAAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAggg                                                                                                               <  2:799035/140‑1 (MQ=255)                             ggATTCACGCCAACCAGCTGACCAGTTGCTGGAACTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAggg                                                                                                               <  1:811689/140‑1 (MQ=255)                                     gCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGatat                                                                                                       <  1:1838821/140‑1 (MQ=255)                                         aCCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGcc                                                                                                   >  2:649065/1‑140 (MQ=255)                                         aCCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGcc                                                                                                   >  1:809831/1‑140 (MQ=255)                                                      ttGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAAc                                                                                      >  2:1273798/1‑140 (MQ=255)                                                       tGCTGGATCGAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGTCCCGTCGCCAGACGAATGAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAAct                                                                                     <  2:456377/140‑2 (MQ=255)                                                       ggCTGGATCAAGGACTCCAGCCCTGAGAATCCCGGCGGCGGTCATCGCCCCCACGACACCCTGACCCGGCGCCAGACGAATTCGCACACTGATCAGCCATGCCATGATGTAGGGCGAGAAATTGCCGTGGGACaacaacc                                                                                     <  2:560795/139‑2 (MQ=255)                                                        gCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACAt                                                                                    >  2:1026479/1‑140 (MQ=255)                                                             aTCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGTACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGa                                                                               >  1:716026/1‑140 (MQ=255)                                                             aTCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGTACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGa                                                                               >  1:715906/1‑140 (MQ=255)                                                             aTCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCAAGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGa                                                                               >  1:1025758/1‑140 (MQ=255)                                                                tAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATgg                                                                            >  2:1302418/1‑140 (MQ=255)                                                                tAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATgg                                                                            >  2:1302419/1‑140 (MQ=255)                                                                 attaTTGCTTCACTGATATGACCGGCTGCGTTCATCCTCGAAACGACACCAGGACCCGTGGCCAGTCGTATTAGCTCAGTGATCAGCCATGCCATGATGTAGGGCGAGTTAATGCCGTGGGACATCAACATGCCGATGgt                                                                           <  2:1918184/137‑1 (MQ=255)                                                                   gATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGgtgt                                                                         <  1:264278/140‑1 (MQ=255)                                                                      tGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGc                                                                      >  2:1202712/1‑140 (MQ=255)                                                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCa                                                                    >  2:1102577/1‑140 (MQ=255)                                                                        cagcaCTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCa                                                                    >  2:1102790/1‑140 (MQ=255)                                                                             cTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGcc                                                               >  1:850022/1‑140 (MQ=255)                                                                              tGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGTACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCg                                                              <  2:715906/140‑1 (MQ=255)                                                                              tGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGTACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCg                                                              <  2:716026/140‑1 (MQ=255)                                                                              tGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCg                                                              <  1:1483701/140‑1 (MQ=255)                                                                                  aaTCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGgtgt                                                          <  1:84849/140‑1 (MQ=255)                                                                                  aaTCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGgtgt                                                          <  1:880636/140‑1 (MQ=255)                                                                                  aaTCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGgtgt                                                          <  2:1016901/140‑1 (MQ=255)                                                                                   aTCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCAAGCCATGATGTAGGGCGAGATATTGCCGTGGTACATCAACATGCCGATGGAGACGCCAATGCCGGTGTc                                                         >  2:1436729/1‑140 (MQ=255)                                                                                     cccGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCga                                                       >  2:18994/1‑140 (MQ=255)                                                                                        ggcggcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCgatga                                                    >  1:902687/1‑140 (MQ=255)                                                                                        ggcggcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCgatga                                                    >  1:581092/1‑140 (MQ=255)                                                                                        ggcggcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCgatga                                                    >  1:48202/1‑140 (MQ=255)                                                                                            gcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGt                                                <  1:670322/140‑1 (MQ=255)                                                                                            gcggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGt                                                <  2:960117/140‑1 (MQ=255)                                                                                                       cGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCa                                     <  2:77670/140‑1 (MQ=255)                                                                                                        gAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCAc                                    <  1:100300/140‑1 (MQ=255)                                                                                                        gAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCAc                                    <  1:25843/140‑1 (MQ=255)                                                                                                              acacCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCAcc                              <  1:1375657/140‑1 (MQ=255)                                                                                                              acacCCTGACCAGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGTTGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCAcc                              <  1:1904717/140‑1 (MQ=255)                                                                                                                            cGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCa                >  1:1881267/1‑140 (MQ=255)                                                                                                                            cGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCa                >  2:626741/1‑140 (MQ=255)                                                                                                                                aGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAAt            <  1:724880/140‑1 (MQ=255)                                                                                                                                  aCGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCa          >  2:1383714/1‑140 (MQ=255)                                                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc         <  1:540081/140‑1 (MQ=255)                                                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc         <  1:1567259/140‑1 (MQ=255)                                                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc         <  1:974122/140‑1 (MQ=255)                                                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc         <  2:10896/140‑1 (MQ=255)                                                                                                                                   cGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCAc         <  1:974227/140‑1 (MQ=255)                                                                                                                                     aaTTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCa       <  1:674971/140‑1 (MQ=255)                                                                                                                                          gCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCa  <  1:1312030/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                           CGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTCAGCACGCCACCCGCACCGATGATCAGAATCACCATTGCA  >  NC_000913/4550115‑4550391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑