| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 987,008 | (T)8→7 | intergenic (‑26/+577) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 987,001 | 0 | T | . | 94.5% | 238.4 / 8.5 | 55 | intergenic (‑19/+584) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base . (30/22); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
ACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAA > NC_000913/986872‑987135 | aCCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGtttttttatga > 1:1749290/1‑136 (MQ=255)aCCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGtttttttatga > 2:765292/1‑136 (MQ=255)aCCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATg > 2:396683/1‑140 (MQ=255)aCCGTACAGATCAACTTTGTTGCCATCTTGGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGtttttttatga > 2:765295/1‑136 (MQ=255) tACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACAcc > 1:546967/1‑140 (MQ=255) tACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACAcc > 1:1404646/1‑140 (MQ=255) aTCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCa > 1:525215/1‑140 (MQ=255) cTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGcc > 1:527216/1‑140 (MQ=255) taGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATa > 2:860098/1‑140 (MQ=255) cTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAAttt > 2:336205/1‑140 (MQ=255) cTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAAttt > 2:1099197/1‑140 (MQ=255) tGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTa < 2:812221/140‑1 (MQ=255) cGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGaa < 2:881749/140‑1 (MQ=255) cGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGaa < 2:1463659/140‑1 (MQ=255) aGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtga > 1:436877/1‑140 (MQ=255) aGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtga < 2:765186/140‑1 (MQ=255) tACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatg < 2:1318637/140‑1 (MQ=255) tACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatg < 2:844650/140‑1 (MQ=255) cTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATgaga > 2:569609/1‑140 (MQ=255) cTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATgaga > 2:1545801/1‑140 (MQ=255) cTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATgaga > 2:1545671/1‑140 (MQ=255) actaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTg < 1:1249644/140‑1 (MQ=255) actaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTg < 1:878021/140‑1 (MQ=255) actaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTg < 2:1153595/140‑1 (MQ=255) actaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTg < 2:1433426/140‑1 (MQ=255) actaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTg < 2:1436301/140‑1 (MQ=255) actaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTg < 1:1249645/140‑1 (MQ=255) actaACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTg < 1:1249668/140‑1 (MQ=255) taACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGgt < 1:936699/140‑1 (MQ=255) taACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGgt < 2:782356/140‑1 (MQ=255) taACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGgt < 1:1124817/140‑1 (MQ=255) taACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGgt > 1:1434128/1‑140 (MQ=255) agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCtt > 1:372241/1‑140 (MQ=255) agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCttt > 1:1040370/1‑140 (MQ=255) agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCttt > 1:8134/1‑140 (MQ=255) agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCttt > 1:1274023/1‑140 (MQ=255) agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCttt > 2:770910/1‑140 (MQ=255) agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCttt > 1:1360086/1‑140 (MQ=255) agCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCttt > 2:657959/1‑140 (MQ=255) gACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATgtgt < 2:104251/140‑1 (MQ=255) aCGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATgtgt < 1:564456/140‑1 (MQ=255) aTCACTGCCAGAATATTGCGGTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATTTGTCTga > 1:1640655/1‑139 (MQ=255) aTCACTGCCAGAATATTGCGCTTCACCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGc > 1:636434/1‑140 (MQ=255) tGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCa > 1:1243372/1‑140 (MQ=255) ccAGAATATTGCGCGTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATc > 1:682743/1‑140 (MQ=255) ttGCGCTTCATCATTATTTTTTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTAAATATTTTCTGTCAATAAAAATTTAGTTAACTATTGATGATTGTTTGGTTTCTTTATGTGTCTGCAGGAATCTTTCCAtt < 1:1090663/140‑1 (MQ=255) ttGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCAGTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCAtt < 2:425432/140‑1 (MQ=255) gcgcTTCAGCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTAAGGAAGTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCAttaa > 2:1588707/1‑138 (MQ=255) catcatTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTaa > 1:1340559/1‑140 (MQ=255) catcatTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTaa > 1:739739/1‑140 (MQ=255) catcatTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTaa > 1:1092476/1‑140 (MQ=255) catcatTATTTATTACCCTCATGG‑TATTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCATTATGTGTCTGCAGGCATCTTTCCATTCAAACTaa > 1:1341012/1‑140 (MQ=255) tttattACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGc < 2:415378/140‑1 (MQ=255) tttattACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGc < 2:1744568/140‑1 (MQ=255) attattaCCCTCATCG‑ATTTATAATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGc < 2:1640655/139‑1 (MQ=255) attaCCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTAc > 1:490039/1‑140 (MQ=255) taCCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCg > 2:1270710/1‑140 (MQ=255) tCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGaaa > 1:1443032/1‑140 (MQ=255) | ACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGATTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAA > NC_000913/986872‑987135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |