Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,2071.G83.1% 218.6 / 45.1 72noncoding (80/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (6/6);  new base G (35/24);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAC  >  NC_000913/4606068‑4606332
                                                                                                                                             |                                                                                                                                
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGTTATCGCCTCGTTTGGTGCG‑a                                                                                                                                   >  2:719781/1‑140 (MQ=255)
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑a                                                                                                                                   >  2:1201444/1‑140 (MQ=255)
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑a                                                                                                                                   >  1:1170830/1‑140 (MQ=255)
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑a                                                                                                                                   <  2:1507791/140‑1 (MQ=255)
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAATTTTTTTGATATCGCCTCGTTTGGTGCG‑a                                                                                                                                   >  1:1412277/1‑140 (MQ=255)
      aTAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑g‑gg‑g‑gg                                                                                                                         <  1:439607/140‑1 (MQ=255)
               gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGa                                                                                                                 <  1:1009186/140‑1 (MQ=255)
                        gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGCATCTTACTTTTTTTGATGTCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACg                                                                                                        >  1:1070063/1‑140 (MQ=255)
                          gACTTCAACCCCCACGTCCGTATGGTCAGTTCCACCTGATGCTAGCGCGTCTAAGATTTCCGCCACGTTCGCACAGTCATCTTACTTTTTTTGTTATATCCTCGTTTGGTGCG‑A‑G‑GGTG‑GGGGACTTGAAGCCCCTCGTc                                                                                                      <  2:1358539/140‑1 (MQ=255)
                                 aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGa                                                                                               >  1:1464651/1‑140 (MQ=255)
                                   ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTGTGGTGCG‑A‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAaca                                                                                            >  1:985874/1‑140 (MQ=255)
                                      cACGTCCGTAAGGACTCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                          <  1:1695664/140‑1 (MQ=255)
                                      cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                          <  1:1390417/140‑1 (MQ=255)
                                       aggTCCGTTAGGTCTCTATCTCCTGATGCTAGCGCGTCTACGCTTTCCGGCTGCTTCGCAGAGTCATCTTTCTTTTTTTGATTTCGCCTCGTTTGGTGCG‑T‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTATGAACACTaa                                                                                        <  2:741555/138‑1 (MQ=21)
                                       aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACTTCCGTAAGAACACTa                                                                                         >  2:1122193/1‑140 (MQ=255)
                                       aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                         >  1:763981/1‑140 (MQ=255)
                                       aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGAAATCGCCTCGTTTGGTGCG‑A‑GTGG‑G‑GGGGAGTTGAACCCCCACGTCCGTAAGAAAACTa                                                                                         >  2:1122225/1‑140 (MQ=37)
                                        cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                        <  1:1009282/140‑1 (MQ=255)
                                             gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                   >  1:425890/1‑140 (MQ=255)
                                               aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                                                 <  1:1706865/140‑1 (MQ=255)
                                                 ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                                               >  2:166091/1‑140 (MQ=255)
                                                 ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                                               >  2:1270442/1‑140 (MQ=255)
                                                 ggACAATAACACCTGAAGCTAGCGCGACTACCAATTCCGCCACCTTCGCACACACATCATACTATATTTGATAACGCCTCGGTTGGTGCG‑A‑G‑GT‑G‑TGGGACTTCAACCCCCACGTCCGTAATAAGAATAACACCTGAAGc                                                                              >  2:23619/1‑140 (MQ=21)
                                                     acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                          >  1:36060/1‑140 (MQ=255)
                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                         >  1:806496/1‑140 (MQ=255)
                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAAAACCTGAAGCTAgcg                                                                         >  1:1606163/1‑140 (MQ=255)
                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑G‑GG‑GTGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                         >  2:610008/1‑140 (MQ=255)
                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTGTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCAGAAGCTAgag                                                                         >  1:645822/1‑138 (MQ=38)
                                                             cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                   >  1:170873/1‑140 (MQ=255)
                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                 >  1:1692785/1‑140 (MQ=255)
                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                >  1:509042/1‑140 (MQ=255)
                                                                 aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCTCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                               >  1:254202/1‑140 (MQ=255)
                                                                     aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                           >  1:1101863/1‑140 (MQ=255)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                         >  1:1069631/1‑140 (MQ=255)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTAGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                         <  1:1138698/140‑1 (MQ=255)
                                                                       cgattCTACCAATTCCGCCACCTTCTCACAGTCATATTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                         <  2:1043994/136‑1 (MQ=21)
                                                                           tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                     >  1:1000371/1‑140 (MQ=255)
                                                                           tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTATTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                     >  1:1631521/1‑140 (MQ=255)
                                                                                 acttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                               <  2:1285859/138‑1 (MQ=255)
                                                                                 aaTTCCGCCACCTTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                               <  1:1248275/140‑1 (MQ=255)
                                                                                 aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                               <  2:1287306/140‑1 (MQ=255)
                                                                                    tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                                            >  2:1535215/1‑140 (MQ=255)
                                                                                      caccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCTATTCCGCCACCTTCGCCCAGTg                                          >  1:1270885/3‑140 (MQ=255)
                                                                                      cGCCCCCTTCGCACAGTCAACTTACTTTTTTTGATATCGCCGCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                          <  2:630663/140‑1 (MQ=21)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGTTATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCGAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                          <  1:42990/140‑1 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                          <  1:1594162/140‑1 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                          <  2:318882/140‑1 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                          <  1:557518/140‑1 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCATCGCCCAGTGCg                                        >  2:1521695/1‑140 (MQ=255)
                                                                                        ccACCTTCGCACAGACATCTTACTTTTTATGATATCGCCTCGATTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCTTAAGAACACTAACACCACAAGCTAGCGCGTCTACCAATACCCCCAACTTCGCCCACTGCg                                        >  2:891833/1‑140 (MQ=18)
                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                      <  1:989404/140‑1 (MQ=255)
                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                      <  2:626219/140‑1 (MQ=255)
                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                      <  1:987240/140‑1 (MQ=255)
                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTAGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCAACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                      <  2:626101/140‑1 (MQ=255)
                                                                                          aCCTACGCACAGTCATCTTACTTTTTTTGAGATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTAGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                      <  2:1542891/140‑1 (MQ=21)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTTAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCAACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                          >  1:1367339/1‑140 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                          >  2:1061419/1‑140 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                          >  2:1061597/1‑140 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                          >  1:257229/1‑140 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                          >  2:1167012/1‑140 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGt                          >  2:615199/1‑140 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAATTTCAACGt                          >  1:1366348/1‑140 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACAAACACCAGAATCTAGCGCGACTACCAATTCCGCCACCTTCTCCCAGAGCGAGAACTATCAACgg                          >  1:1370124/1‑139 (MQ=18)
                                                                                                      tCATCTTACTTTTATTGATATCGACTCGTTAGGTGCG‑AGG‑GG‑G‑GGGGAAATTAACCCCCACGTCCGTAAGAACACAAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTGCGAGCAATATCAACGt                          >  2:615330/1‑140 (MQ=18)
                                                                                                        aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTgg                        >  1:446657/1‑140 (MQ=255)
                                                                                                        aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGTgg                        >  1:1525647/1‑140 (MQ=255)
                                                                                                           ttACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                    <  2:1688901/140‑1 (MQ=255)
                                                                                                            tACTTTTTTTGATATCGCCTCGTTTGGTGCGTG‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                    <  2:558212/140‑1 (MQ=255)
                                                                                                            tACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt                   <  1:1014036/140‑1 (MQ=255)
                                                                                                             aCTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGCGAGCAATATCAACGTGGttttt                   <  2:263445/140‑1 (MQ=255)
                                                                                                             aCTATTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt                   <  2:263367/140‑1 (MQ=255)
                                                                                                              cTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                  >  2:101035/1‑140 (MQ=255)
                                                                                                                ttttttGATATCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                >  1:1097778/1‑140 (MQ=255)
                                                                                                                   tttGTTATCGACTAGTTTGGTGCG‑ATG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAAAACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg             <  2:801430/140‑1 (MQ=21)
                                                                                                                        tatCGCCTGGTGTGGTGCG‑T‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCTTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg       <  2:45922/140‑1 (MQ=21)
                                                                                                                          tCGCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg      <  1:1359893/140‑1 (MQ=255)
                                                                                                                            gCCTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGATCAATATCAACGTGGTTTTTGGTGCGAgggggggg    >  2:203553/1‑140 (MQ=255)
                                                                                                                              cTCGTTTGGTGCG‑AGG‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc  <  1:146809/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                
GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑G‑GG‑G‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAC  >  NC_000913/4606068‑4606332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑