Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,147,021 T→C M174V (ATG→GTG)  alkA ← 3‑methyl‑adenine DNA glycosylase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,147,0210TC95.2% 298.0 / 3.6 83M174V (ATG→GTG) alkA3‑methyl‑adenine DNA glycosylase II
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (33/46);  total (35/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGCC  >  NC_000913/2146888‑2147157
                                                                                                                                     |                                                                                                                                        
cAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTaa                                                                                                                                    <  2:166140/140‑1 (MQ=255)
cAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTaa                                                                                                                                    <  1:92556/140‑1 (MQ=255)
cAGCGCCCGAGACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTaa                                                                                                                                    <  1:407822/140‑1 (MQ=255)
        gATATCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATTAATGAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTa                                                                                                                            <  2:1290305/140‑1 (MQ=255)
        gATACCCTGAAAAGTTTGCAGCGTTTACATCGCCAGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCATCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTa                                                                                                                            <  2:533423/140‑1 (MQ=255)
        gATACCCGGAAATGTTTGAAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTa                                                                                                                            <  2:1700169/140‑1 (MQ=255)
        gATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTa                                                                                                                            <  1:813995/140‑1 (MQ=255)
          tACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAAt                                                                                                                          >  1:47034/1‑140 (MQ=255)
                gAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGc                                                                                                                    >  2:753271/1‑140 (MQ=255)
                gAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAAAGCCTGc                                                                                                                    >  2:753279/1‑140 (MQ=255)
                gAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCAGTGGTAAGGTGCCCTCCAGCGCCGCAATAGCCAGATGAATCAGCGCCTCTGCCCGTTACAACGGCACACCTAACGCTTTTAATGCCTGc                                                                                                                    >  2:753273/1‑140 (MQ=255)
                    aGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGt                                                                                                                <  1:770958/140‑1 (MQ=255)
                    aGTTTGCAGCGTTTTCATCGCCTGATCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGt                                                                                                                <  2:894349/140‑1 (MQ=255)
                                       gCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTg                                                                                             >  1:455214/1‑140 (MQ=255)
                                       gCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTg                                                                                             >  2:1111347/1‑140 (MQ=255)
                                         cTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAg                                                                                           >  1:1080243/1‑140 (MQ=255)
                                            cTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCg                                                                                        >  1:1715659/1‑140 (MQ=255)
                                               cacaTCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCg                                                                                     <  2:177693/140‑1 (MQ=255)
                                               cacaTCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCg                                                                                     <  2:177564/140‑1 (MQ=255)
                                                  aTCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGa                                                                                  <  2:673106/140‑1 (MQ=255)
                                                  aTCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGa                                                                                  <  1:212218/140‑1 (MQ=255)
                                                  aTCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGa                                                                                  <  1:213228/140‑1 (MQ=255)
                                                    cGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAg                                                                                <  2:208058/140‑1 (MQ=255)
                                                    cGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAg                                                                                <  2:1029774/140‑1 (MQ=255)
                                                    cGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAg                                                                                <  2:1293060/140‑1 (MQ=255)
                                                    cGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAg                                                                                <  2:741793/140‑1 (MQ=255)
                                                    cGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAg                                                                                <  1:1143841/140‑1 (MQ=255)
                                                    cGCCCGGTATTGACATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAg                                                                                <  2:207905/140‑1 (MQ=255)
                                                      cccGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCa                                                                              >  2:241824/1‑140 (MQ=255)
                                                      cccGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCa                                                                              >  1:1756489/1‑140 (MQ=255)
                                                      cccGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCa                                                                              >  1:634877/1‑140 (MQ=255)
                                                            aTTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGAtata                                                                        <  1:1146174/140‑1 (MQ=255)
                                                            aTTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGAtata                                                                        <  1:1286832/140‑1 (MQ=255)
                                                              tGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACt                                                                      >  1:136024/1‑140 (MQ=255)
                                                              tGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACt                                                                      >  1:136105/1‑140 (MQ=255)
                                                                tCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTcc                                                                    >  1:160257/1‑140 (MQ=255)
                                                                tCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCAAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTTCCAGCCGCTGAGGCGTCGGTAAGCAGATATACTcc                                                                    >  1:1596844/1‑140 (MQ=255)
                                                                 cATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCg                                                                   >  2:677620/1‑140 (MQ=255)
                                                                    tGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGTGGCGTCGGGAAGCAGATATACTCCGGaa                                                                >  1:1694135/1‑140 (MQ=255)
                                                                     ggTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGACGCAGATATACTCCGGaaa                                                               >  2:1483546/1‑140 (MQ=255)
                                                                     ggTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGaaa                                                               >  1:1297067/1‑140 (MQ=255)
                                                                     ggTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGaaa                                                               >  1:578085/1‑140 (MQ=255)
                                                                     ggTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGaaa                                                               >  1:577893/1‑140 (MQ=255)
                                                                     ggTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTAAATGCCTGCGGGTCTGCTGCAGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGaaa                                                               >  1:942861/1‑140 (MQ=255)
                                                                          ggTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAatcat                                                          >  1:1552896/1‑140 (MQ=255)
                                                                          ggTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAatcat                                                          >  1:1223465/1‑140 (MQ=255)
                                                                          ggTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAatcat                                                          >  1:1552889/1‑140 (MQ=255)
                                                                              cccTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAg                                                      >  1:1538827/1‑140 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  1:1145004/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  1:269282/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTACTGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  2:176486/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  2:634877/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  2:1062810/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  2:718816/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  2:719100/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  1:828480/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  1:794683/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGtt                                                 <  1:734093/140‑1 (MQ=255)
                                                                                   cAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTAAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCCtt                                                 <  2:1065824/140‑1 (MQ=255)
                                                                                     gcgcCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCg                                               <  2:507824/140‑1 (MQ=255)
                                                                                     gcgcCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATAATCCAGCCGTTCg                                               >  2:428209/1‑140 (MQ=255)
                                                                                           cATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATaa                                         >  2:129705/1‑140 (MQ=255)
                                                                                             tttGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTCACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAg                                       <  2:922510/140‑1 (MQ=255)
                                                                                             tttGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAg                                       <  2:1756489/140‑1 (MQ=255)
                                                                                             tttGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCCAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAACTCATCCAGCCGTTCGCCATaacg                                       <  2:1243300/140‑3 (MQ=255)
                                                                                                gCCAGATGACTCAGCGCCTCTGCCCTTTTCAACGGCACACCTAACGCTTGTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCGGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCtg                                    <  2:1392262/140‑1 (MQ=255)
                                                                                                gCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCtg                                    <  1:1570469/140‑1 (MQ=255)
                                                                                                gCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCtg                                    <  1:1686522/140‑1 (MQ=255)
                                                                                                gCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCtg                                    <  2:1520263/140‑1 (MQ=255)
                                                                                                gCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCtg                                    <  2:1578830/140‑1 (MQ=255)
                                                                                                   aGATGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGc                                 <  2:909368/140‑1 (MQ=255)
                                                                                                    gttGAATCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGATCTCCATAAAGCTGTGcc                                >  2:878154/3‑140 (MQ=255)
                                                                                                          tCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTg                          >  1:134411/1‑140 (MQ=255)
                                                                                                          tCAGCGCCTCTGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTg                          >  1:138226/1‑140 (MQ=255)
                                                                                                                   ctGCCCGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAAt                 >  1:685336/1‑140 (MQ=255)
                                                                                                                      cccGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAAtttt              >  1:271604/1‑140 (MQ=255)
                                                                                                                      cccGTTTCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAAtttt              >  1:195655/1‑140 (MQ=255)
                                                                                                                           ttCAACGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTgccgc         >  1:1060584/1‑140 (MQ=255)
                                                                                                                                cGGCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCg    <  2:911962/140‑1 (MQ=255)
                                                                                                                                  gCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCTCTCTGGCGGTCAATTTTGCCGCCATCGcc  <  2:1167654/140‑1 (MQ=255)
                                                                                                                                  gCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGcc  <  2:1167626/140‑1 (MQ=255)
                                                                                                                                  gCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGcc  <  1:365756/140‑1 (MQ=255)
                                                                                                                                  gCACACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGcc  <  1:365677/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
CAGCGCCCGATACCCGGAAAAGTTTGCAGCGTTTTCATCGCCTGCTCCACATCGCCCGGTATTGTCATTGGTAAGGTGCCCTCCAGCGCCGCATTTGCCAGATGAATCAGCGCCTCTGCCCGTTTCAACGGCATACCTAACGCTTTTAATGCCTGCGGGTCTGCTGCTGCCAGCCGCTGAGGCGTCGGGAAGCAGATATACTCCGGAAAATCATCCAGCCGTTCGCCATAAAGCTGTGCCACTCTGGCGGTCAATTTTGCCGCCATCGCC  >  NC_000913/2146888‑2147157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: