Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,471,252 T→C N89S (AAC→AGC)  yfdK ← CPS‑53 (KpLE1) prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,471,2520TC98.7% 301.4 / ‑4.1 80N89S (AAC→AGC) yfdKCPS‑53 (KpLE1) prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (51/27);  total (53/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGTTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTCGGCGGTA  >  NC_000913/2471120‑2471384
                                                                                                                                    |                                                                                                                                    
ggCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTg                                                                                                                               >  1:1121322/1‑140 (MQ=255)
ggCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTg                                                                                                                               >  2:1085033/1‑140 (MQ=255)
ggCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTg                                                                                                                               >  1:1124520/1‑140 (MQ=255)
     aCTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTaa                                                                                                                          >  2:196173/1‑140 (MQ=255)
               tCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTATTCAATtgctg                                                                                                                >  2:1559736/1‑140 (MQ=255)
               tCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATtgctg                                                                                                                >  2:920270/1‑140 (MQ=255)
               tCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATtgctg                                                                                                                >  2:1046909/1‑140 (MQ=255)
               tCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTATACTGTTCATATAATCGCTGGCCTGATTAAACAAGtgctg                                                                                                                >  2:636972/1‑140 (MQ=255)
               tCGACCAGATCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACATTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGGTCATATATTCGCTGGCCTGATTAATCAATtgctg                                                                                                                >  2:754710/1‑140 (MQ=39)
                 gACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTgctgct                                                                                                              >  2:1325389/1‑140 (MQ=255)
                 gACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTgctgct                                                                                                              >  1:98478/1‑140 (MQ=255)
                 gACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTgctgct                                                                                                              >  1:98377/1‑140 (MQ=255)
                   ccAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCttt                                                                                                            <  2:1745178/140‑1 (MQ=255)
                   ccAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCttt                                                                                                            >  1:1391640/1‑140 (MQ=255)
                       cTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCa                                                                                                        >  2:1176794/1‑140 (MQ=255)
                       cTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCAATGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCa                                                                                                        >  2:326452/1‑140 (MQ=255)
                                 tCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTTGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTgc                                                                                              >  2:1531676/1‑140 (MQ=255)
                                 tCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTgc                                                                                              >  1:1039983/1‑140 (MQ=255)
                                 tCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCAGTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTgc                                                                                              >  1:1029187/1‑140 (MQ=255)
                                 tCCAGATAATCCAGCCACAAATTATATAGCGCCAGTTCCTCACATTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACAGTTCATAAAATCGCTGGCCTGATAAATCAAATGCTGCTTTTTCAGTTCGGCTgc                                                                                              >  2:767107/1‑140 (MQ=255)
                                  ccAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTgca                                                                                             <  1:547198/140‑1 (MQ=255)
                                        aaTCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATc                                                                                       <  1:589637/140‑1 (MQ=255)
                                          tCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTg                                                                                     <  2:972188/140‑1 (MQ=255)
                                          tCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTg                                                                                     <  2:1150327/140‑1 (MQ=255)
                                          tCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTg                                                                                     <  1:971330/140‑1 (MQ=255)
                                          tCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTg                                                                                     <  1:970893/140‑1 (MQ=255)
                                          tCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTg                                                                                     <  1:1395720/140‑1 (MQ=255)
                                              gCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTcc                                                                                 >  2:643506/1‑140 (MQ=255)
                                               ccACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCt                                                                                >  1:1540514/1‑140 (MQ=255)
                                               ccACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCt                                                                                <  1:625202/140‑1 (MQ=255)
                                               ccACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCt                                                                                >  2:1709627/1‑140 (MQ=255)
                                               ccACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCt                                                                                <  2:488364/140‑1 (MQ=255)
                                               ccACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCt                                                                                <  1:186141/140‑1 (MQ=255)
                                               ccACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCt                                                                                >  1:1692495/1‑140 (MQ=255)
                                               ccACAAATTATAGTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCt                                                                                <  1:1670467/140‑1 (MQ=255)
                                                cacaAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTc                                                                               >  1:795274/1‑140 (MQ=255)
                                                cacaAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTc                                                                               >  2:1088244/1‑140 (MQ=255)
                                                      ttATATTGCGCCTGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATTATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATgtgt                                                                         <  2:1222514/140‑1 (MQ=255)
                                                      ttATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATgtgt                                                                         <  2:1222569/140‑1 (MQ=255)
                                                      ttATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATgtgt                                                                         <  2:1222564/140‑1 (MQ=255)
                                                          atTGCGCCAGTTCCTCACCATTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTAAAGGTCATATAAACGATGGCCTGATTAAACAATAGCAGCTTTTACAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTtggt                                                                     >  2:1229507/1‑140 (MQ=255)
                                                            tGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTtggtgg                                                                   >  2:351445/1‑140 (MQ=255)
                                                              cgcCAGTTCCTCCCCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTtggtggtg                                                                 <  1:883945/140‑1 (MQ=255)
                                                              cgcCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTtggtggtg                                                                 <  1:1379513/140‑1 (MQ=255)
                                                              cgcCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTtggtggtg                                                                 <  1:1381637/140‑1 (MQ=255)
                                                              cgcCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCAGTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTtggtggtg                                                                 >  2:599347/1‑140 (MQ=255)
                                                              cgcAAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTAATGTTCATATAATCGCTGGCCTGATTAATCAATAGCTGGTTTATCAGTTCTGCTGCAGCAATCTGTTCCTCATGTGTtggtggtg                                                                 >  2:657994/1‑140 (MQ=255)
                                                                ccAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGa                                                               >  2:1189277/1‑140 (MQ=255)
                                                                      ccTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCa                                                         <  1:858954/140‑1 (MQ=255)
                                                                         cACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGAc                                                      >  1:1226281/1‑140 (MQ=255)
                                                                              ttCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGc                                                 >  2:1547549/1‑140 (MQ=255)
                                                                              ttCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGc                                                 >  2:1547647/1‑140 (MQ=255)
                                                                                     gacCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGaaaa                                          <  1:337799/140‑1 (MQ=255)
                                                                                      acCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAAc                                         >  1:205949/1‑140 (MQ=255)
                                                                                      acCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAAc                                         >  1:119204/1‑140 (MQ=255)
                                                                                      acCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAAc                                         >  1:119129/1‑140 (MQ=255)
                                                                                      acCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAAc                                         >  1:1412763/1‑140 (MQ=255)
                                                                                      acCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAAc                                         >  1:1744954/1‑140 (MQ=255)
                                                                                          aTAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCAtt                                     <  1:1500237/140‑1 (MQ=255)
                                                                                          aTAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCAtt                                     <  1:297462/140‑1 (MQ=255)
                                                                                                 cTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCa                              >  1:1124385/1‑140 (MQ=255)
                                                                                                 cTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTATCCa                              >  2:914955/1‑140 (MQ=255)
                                                                                                 cTTTACCAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAAACAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTAAGACCATGCAGGAAAACCATTTTATCCa                              >  1:1124105/1‑140 (MQ=255)
                                                                                                   ttACCAGCCCATTGTTTACTGTTCATATAATCGTTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGc                            >  1:554672/1‑140 (MQ=255)
                                                                                                       cAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATa                        <  1:1481070/140‑1 (MQ=255)
                                                                                                       cAGCCCATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATa                        <  1:1666988/140‑1 (MQ=255)
                                                                                                          cccATTGTTTACTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACgg                     >  1:694447/1‑140 (MQ=255)
                                                                                                                    aCTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCtt           >  1:192461/1‑140 (MQ=255)
                                                                                                                    aCTGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTACTCATGTGTTGGTGTTGGAATTTCAGACCATGCAGGAACACCATTTTCTCCAGCGATACGGAATTATCCtt           >  2:477828/1‑140 (MQ=255)
                                                                                                                      tGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTc         >  2:1729546/1‑140 (MQ=255)
                                                                                                                      tGTTCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTc         >  2:721841/1‑140 (MQ=255)
                                                                                                                        ttCATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTcgg       >  1:1517136/1‑140 (MQ=255)
                                                                                                                          cATATAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTcggcg     >  1:1223732/1‑140 (MQ=255)
                                                                                                                           atatACTCGCTGGCCTGGTTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTGCTCTTGTGTTGGTGGTGGAATTTCAGACCATGCAGGTAAACCATTTTCTCCAGCGAGACGGATTTTTCCTATcggcgg    >  2:1492491/1‑140 (MQ=255)
                                                                                                                           atatAATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTcggcgg    >  2:1706488/1‑140 (MQ=255)
                                                                                                                           ataaAATCGCTGGCCTGATTAATCAATTGCTGCTTTATCAGTACGGCTGCAGCAATCTGTTCCTCATGTGATGGAGGTGGAATATCAGACCATGCAGGAAAACCATTTTCTACAGCGAAACTGATTTTTCCTTAaggcgg    >  2:49336/1‑140 (MQ=255)
                                                                                                                             ataATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTCGGCGGTa  <  2:555689/140‑1 (MQ=255)
                                                                                                                             ataATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTCGGCGGTa  <  2:648576/140‑1 (MQ=255)
                                                                                                                             ataATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTCGGCGGTa  >  2:660660/1‑140 (MQ=255)
                                                                                                                             ataATCGCTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTCGGCGGTa  <  2:1213844/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTGTTTACTGTTCATATAATCGTTGGCCTGATTAATCAATTGCTGCTTTTTCAGTTCGGCTGCAGCAATCTGTTCCTCATGTGTTGGTGGTGGAATTTCAGACCATGCAGGAAAACCATTTTCTCCAGCGATACGGATTTTTCCTTTCGGCGGTA  >  NC_000913/2471120‑2471384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: