Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,821,424 G→C G196G (GGC→GGG)  alaS ← alanyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,821,4240GC93.1% 377.3 / 15.5 101G196G (GGC→GGG) alaSalanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base C (46/48);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 101 total aligned reads displayed.

CTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTT  >  NC_000913/2821286‑2821557
                                                                                                                                           |                                                                                                                                     
cTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑Gc                                                                                                                                      <  1:731194/140‑1 (MQ=255)
   cAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑Ccccc                                                                                                                                   >  2:669628/1‑140 (MQ=255)
   cAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑Ccccc                                                                                                                                   >  1:694285/1‑140 (MQ=255)
   cAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTAGCCGTCTTCTTCCGGGCTTCCCGGAGG‑Ccccc                                                                                                                                   >  1:966372/1‑140 (MQ=255)
         cATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCaaa                                                                                                                             >  2:683992/1‑140 (MQ=255)
         cATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCaaa                                                                                                                             <  1:1438386/140‑1 (MQ=255)
         cATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAAAGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCaaa                                                                                                                             <  2:1538146/140‑1 (MQ=255)
          aTACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGACATCGGCCTGGCGGTGGAACTGCAAGAAGACGATGTTCCAGATCGCAAGGTAGCGGGCGCCGTCGTCTTCCGGGCTGCCCGGGGG‑GCCCCCCCgaag                                                                                                                            >  1:870806/1‑139 (MQ=255)
           tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                           >  1:353606/1‑140 (MQ=255)
           tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                           >  2:1396734/1‑140 (MQ=255)
           tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                           >  1:1473420/1‑140 (MQ=255)
           tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                           >  1:73639/1‑140 (MQ=255)
           tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                           >  1:73640/1‑140 (MQ=255)
           tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                           >  2:366859/1‑140 (MQ=255)
           tACCGGTATCTACAGACGGCTTCGGCAGCGGGTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                           >  2:366874/1‑140 (MQ=255)
             ccGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTCGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtgtg                                                                                                                         <  1:623562/140‑1 (MQ=255)
             ccGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtgtg                                                                                                                         <  1:428430/140‑1 (MQ=255)
                   tcgaCAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCTTGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGcc                                                                                                                   <  2:1549751/137‑1 (MQ=255)
                     tACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGt                                                                                                                 <  2:1631804/140‑1 (MQ=255)
                     tACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGt                                                                                                                 <  2:1231550/140‑1 (MQ=255)
                     tACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGt                                                                                                                 <  2:453447/140‑1 (MQ=255)
                          aCGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCg                                                                                                            >  1:1190789/1‑140 (MQ=255)
                             gCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                                                                         >  2:1005077/1‑140 (MQ=255)
                             gCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                                                                         >  2:1002672/1‑140 (MQ=255)
                             gCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                                                                         >  2:1005851/1‑140 (MQ=255)
                             gCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                                                                         >  1:1644171/1‑140 (MQ=255)
                              cTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTaga                                                                                                        >  1:1119583/1‑140 (MQ=255)
                               ttCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTagaa                                                                                                       >  1:1112932/1‑140 (MQ=255)
                                     aGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTc                                                                                                 <  1:1539965/140‑1 (MQ=255)
                                     aGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTc                                                                                                 <  2:348051/140‑1 (MQ=255)
                                               tCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                                                                       <  1:904681/140‑1 (MQ=255)
                                               tCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                                                                       <  1:1137188/140‑1 (MQ=255)
                                               tCGTGCCATCGGCCTGGCAGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                                                                       <  1:1711362/140‑1 (MQ=255)
                                               tCGTGCCATCGGCCTGGCAGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                                                                       <  1:1710857/140‑1 (MQ=255)
                                                  tGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCg                                                                                    >  1:1629799/1‑140 (MQ=255)
                                                   gCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGc                                                                                   <  2:1611632/140‑1 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  <  1:242497/140‑1 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  <  1:242518/140‑1 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  <  1:1049576/140‑1 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  <  2:1475080/140‑1 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  <  2:264298/140‑1 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  >  1:578134/1‑140 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  <  1:18297/140‑1 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  <  1:1750477/140‑1 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  <  2:263988/140‑1 (MQ=255)
                                                    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                  <  2:262504/140‑1 (MQ=255)
                                                         ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                             <  2:1125093/140‑1 (MQ=255)
                                                         ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                             <  2:665497/140‑1 (MQ=255)
                                                         ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                             <  2:1125084/140‑1 (MQ=255)
                                                         ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                             <  2:1406164/140‑1 (MQ=255)
                                                            cTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAg                                                                          <  1:1663570/140‑1 (MQ=255)
                                                            cTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAg                                                                          <  1:1665634/140‑1 (MQ=255)
                                                               gCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgtgt                                                                       >  1:1151276/1‑140 (MQ=255)
                                                                    tGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAccc                                                                  >  1:1551450/1‑140 (MQ=255)
                                                                           cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGcc                                                           >  2:1700303/1‑140 (MQ=255)
                                                                           cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGcc                                                           >  1:138319/1‑140 (MQ=255)
                                                                           cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGcc                                                           >  2:1450454/1‑140 (MQ=255)
                                                                           cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGcc                                                           >  2:1450338/1‑140 (MQ=255)
                                                                           cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCCGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGGGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGcc                                                           >  2:907046/1‑140 (MQ=255)
                                                                               aaGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa                                                       <  2:1013646/140‑1 (MQ=255)
                                                                               aaGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa                                                       <  1:1585393/140‑1 (MQ=255)
                                                                               aaGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa                                                       <  1:1585410/140‑1 (MQ=255)
                                                                                aGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAg                                                      >  1:1510834/1‑140 (MQ=255)
                                                                                    gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGt                                                  <  1:428849/140‑1 (MQ=255)
                                                                                    gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGt                                                  <  2:1112932/140‑1 (MQ=255)
                                                                                     aTGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTc                                                 >  2:292442/1‑140 (MQ=255)
                                                                                      tGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCa                                                >  1:622129/1‑140 (MQ=255)
                                                                                         tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGAt                                             >  1:1282520/1‑140 (MQ=255)
                                                                                            aGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCACCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCg                                          <  1:1019113/140‑1 (MQ=255)
                                                                                                ctcAATGTAGCGGTCGCCGTCTTCTTCCGTGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAACTTGTCAGATGCGTATg                                      <  1:587652/140‑1 (MQ=255)
                                                                                                ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATg                                      >  1:759366/1‑140 (MQ=255)
                                                                                                 tcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAGGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATgg                                     <  1:1114481/140‑1 (MQ=255)
                                                                                                  cAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGgc                                    >  2:1684608/1‑140 (MQ=255)
                                                                                                             tCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGtt                         >  1:560559/1‑140 (MQ=255)
                                                                                                             tCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGtt                         >  1:1739541/1‑140 (MQ=255)
                                                                                                             tCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGtt                         >  2:354835/1‑140 (MQ=255)
                                                                                                               gCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTAt                       >  2:560581/1‑140 (MQ=255)
                                                                                                               gCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTAt                       >  2:650759/1‑140 (MQ=255)
                                                                                                                     ttcttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGa                 <  1:1054869/140‑1 (MQ=255)
                                                                                                                     ttcttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGa                 <  1:1051811/140‑1 (MQ=255)
                                                                                                                     ttcttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGa                 <  1:1054901/140‑1 (MQ=255)
                                                                                                                      tcttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt                <  1:1002672/140‑1 (MQ=255)
                                                                                                                      tcttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt                <  1:1005851/140‑1 (MQ=255)
                                                                                                                      tcttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt                <  1:1005077/140‑1 (MQ=255)
                                                                                                                      tcttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt                <  1:767086/140‑1 (MQ=255)
                                                                                                                      tcttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt                <  1:767094/140‑1 (MQ=255)
                                                                                                                       cttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATg               <  1:1239181/140‑1 (MQ=255)
                                                                                                                       cttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATg               <  1:2084/140‑1 (MQ=255)
                                                                                                                       cttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATg               >  1:730110/1‑140 (MQ=255)
                                                                                                                       cttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATATGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATg               <  2:1301762/140‑1 (MQ=255)
                                                                                                                        ttcCGGGCTTCCCGGAGGCCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATg               <  2:385748/140‑1 (MQ=255)
                                                                                                                         tcCGGGCTTCCCGGAGG‑CCCCCCCCAACTGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCg             <  1:683992/140‑1 (MQ=255)
                                                                                                                          ccGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTCTCGCCGATGCGa            >  2:384412/1‑140 (MQ=255)
                                                                                                                           cGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaa           >  1:1439707/1‑140 (MQ=255)
                                                                                                                           cGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaa           >  1:1557474/1‑140 (MQ=255)
                                                                                                                           cGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaa           >  1:825705/1‑140 (MQ=255)
                                                                                                                             ggCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaata         >  2:1124032/1‑140 (MQ=255)
                                                                                                                             ggCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaata         >  2:1121921/1‑140 (MQ=255)
                                                                                                                               cTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaataat       >  2:546543/1‑140 (MQ=255)
                                                                                                                                    cGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGtt  <  1:1156417/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
CTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTT  >  NC_000913/2821286‑2821557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑