Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,285,364	T→C	intergenic (‑151/+49)	yjcH ← / ← acs	DUF485 family inner membrane protein/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,364	0	T	C	100.0%	378.6 / NA	97	intergenic (‑151/+49)	yjcH/acs	DUF485 family inner membrane protein/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/42);  total (55/42)

GGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTG  >  NC_000913/4285229‑4285500                                                                                                                                        |                                                                                                                                         ggTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTa                                                                                                                                      >  2:1400275/1‑140 (MQ=255) ggTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTa                                                                                                                                      >  2:1285925/1‑140 (MQ=255)  gTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTAt                                                                                                                                     <  2:160985/140‑1 (MQ=255)  gTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTAt                                                                                                                                     <  2:1600443/140‑1 (MQ=255)  gTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTAt                                                                                                                                     <  2:1600324/140‑1 (MQ=255)  gTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTAt                                                                                                                                     >  2:1575897/1‑140 (MQ=255)     aTGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAATGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAg                                                                                                                                  >  1:1381561/1‑140 (MQ=255)     aTGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAg                                                                                                                                  <  1:1071873/140‑1 (MQ=255)     aTGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAg                                                                                                                                  >  1:1488664/1‑140 (MQ=255)     aTGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAg                                                                                                                                  <  1:1071617/140‑1 (MQ=255)     aTGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAg                                                                                                                                  >  2:1336926/1‑140 (MQ=255)     aTGTAGGGATTGTTTTAAGTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAg                                                                                                                                  <  1:1071621/140‑1 (MQ=255)      tGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAgg                                                                                                                                 <  2:1321603/140‑1 (MQ=255)       gTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGc                                                                                                                                <  2:1488664/140‑1 (MQ=255)          gggATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTa                                                                                                                             >  2:1749746/1‑140 (MQ=255)          gggATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTa                                                                                                                             >  2:1560155/1‑140 (MQ=255)             aTTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACaa                                                                                                                          >  2:68612/1‑140 (MQ=255)                    tAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTa                                                                                                                   <  2:1064710/140‑1 (MQ=255)                    tAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTa                                                                                                                   <  2:1722951/140‑1 (MQ=255)                    tAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTa                                                                                                                   <  2:1723654/140‑1 (MQ=255)                          ccGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACt                                                                                                             <  2:32798/140‑1 (MQ=255)                           cGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTc                                                                                                            >  1:1474027/1‑140 (MQ=255)                           cGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTc                                                                                                            >  1:726849/1‑140 (MQ=255)                                ccTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATc                                                                                                       >  1:703200/1‑140 (MQ=255)                                ccTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATc                                                                                                       >  1:704253/1‑140 (MQ=255)                                        gagaAGGTTAACGCACGTTTAAAAATTTAAAAAAGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAAtt                                                                                               >  2:1099751/1‑140 (MQ=18)                                          gaAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATtgt                                                                                             >  2:1511309/1‑140 (MQ=255)                                          gaAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCGTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATtgt                                                                                             >  1:990266/1‑140 (MQ=255)                                          gaAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGATGTCGCGCCTTATCAGGACTACAAACCGTTACCGACTCGCATCGGGCAATtgt                                                                                             >  2:1478436/1‑140 (MQ=255)                                              gTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGt                                                                                         <  2:751664/140‑1 (MQ=255)                                                   cGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGa                                                                                    >  2:1703273/1‑140 (MQ=255)                                                      tCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATgg                                                                                 <  2:1090534/140‑1 (MQ=255)                                                        gggTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCCATTGTGGGTTACGATGGCa                                                                               >  2:1132797/1‑140 (MQ=255)                                                        gggTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCa                                                                               >  1:512203/1‑140 (MQ=255)                                                        gggTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCa                                                                               >  1:3684/1‑140 (MQ=255)                                                        gggTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCa                                                                               <  1:63229/140‑1 (MQ=255)                                                        gggTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCa                                                                               <  1:63262/140‑1 (MQ=255)                                                        gggTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCa                                                                               >  2:1132971/1‑140 (MQ=255)                                                        gggTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCa                                                                               <  2:691170/140‑1 (MQ=255)                                                        gggTAACCCTTGCCGAATGTAGGCAGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCAGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCACATGTGGGTTACGAAGGCa                                                                               >  1:512122/1‑140 (MQ=25)                                                                cTTGCCGTCTGTAGGCCGGATAAGGCGATTACGCCGCCTCCGGCAATCACTGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTTCATACAGTTACCGACTCGCATCTGGCAATTGTGTGTTTCGATGGCATCGCGATa                                                                       <  2:1369033/140‑1 (MQ=21)                                                                cTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATa                                                                       <  2:210012/140‑1 (MQ=255)                                                                  tGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGTTGGCATCGCGATAGc                                                                     >  2:998797/1‑140 (MQ=255)                                                                  tGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGc                                                                     >  2:998808/1‑140 (MQ=255)                                                                  tGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGc                                                                     >  1:1250722/1‑140 (MQ=255)                                                                  tGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGc                                                                     >  1:610151/1‑140 (MQ=255)                                                                  tGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGc                                                                     >  1:609960/1‑140 (MQ=255)                                                                   gCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGcc                                                                    <  2:972221/140‑1 (MQ=255)                                                                   gCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGcc                                                                    <  2:790379/140‑1 (MQ=255)                                                                   gCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGcc                                                                    >  1:16558/1‑140 (MQ=255)                                                                   gCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGcc                                                                    >  1:17949/1‑140 (MQ=255)                                                                    ccGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCt                                                                   >  2:1680151/1‑140 (MQ=255)                                                                       aaTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCt                                                                <  1:1555368/140‑1 (MQ=255)                                                                         tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTtc                                                              >  2:1024908/1‑140 (MQ=255)                                                                         tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTtc                                                              >  2:1024962/1‑140 (MQ=255)                                                                         tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTAACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGTTAGCCAGCTtc                                                              >  2:642110/1‑140 (MQ=255)                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCAGACGCGCCTGATCCGGCCTACAAACCCATACCGACTCGCATCCCGCAATTGTCGGTTACGATCGCATCGCGAAAGCCAGCTTctcta                                                          >  2:1406195/1‑139 (MQ=21)                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGTTGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAagc                                                     >  2:650027/1‑140 (MQ=255)                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAagc                                                     <  1:1232547/140‑1 (MQ=255)                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAagc                                                     <  2:739547/140‑1 (MQ=255)                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAagc                                                     <  1:1232681/140‑1 (MQ=255)                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAagc                                                     >  2:650094/1‑140 (MQ=255)                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAagc                                                     <  1:1749746/140‑1 (MQ=255)                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAagc                                                     <  1:1560155/140‑1 (MQ=255)                                                                                   aTAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAagca                                                    >  2:17686/1‑140 (MQ=255)                                                                                   aTAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAagca                                                    >  1:1619181/1‑140 (MQ=255)                                                                                       ggCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCtt                                                <  2:980630/140‑1 (MQ=255)                                                                                        gCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTtc                                               <  1:1266732/140‑1 (MQ=255)                                                                                        gCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTtc                                               <  1:1446222/140‑1 (MQ=255)                                                                                             tACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCACTTGTGGGTTACGATGGCTTCGCGATAGCCTGCGTCTCTTCAAGCAGCGTCGCGAc                                          >  1:1715705/1‑140 (MQ=37)                                                                                             tACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGAc                                          <  1:1418714/140‑1 (MQ=255)                                                                                             tACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGAc                                          >  1:360793/1‑140 (MQ=255)                                                                                             tACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGAc                                          <  1:1421353/140‑1 (MQ=255)                                                                                             tACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGAc                                          >  1:360742/1‑140 (MQ=255)                                                                                             tACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTAACAGGCCTACAAACCGTTACCGACTCGCAACGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGAc                                          >  1:598537/1‑140 (MQ=255)                                                                                               cgccgcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACtg                                        <  2:1463444/140‑2 (MQ=255)                                                                                               cgccgcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACtg                                        <  2:1463445/140‑2 (MQ=255)                                                                                               cgccgcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACtg                                        <  2:336732/140‑2 (MQ=255)                                                                                                   gcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTAACAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGAATCTCGATATCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGcc                                    >  2:893338/1‑140 (MQ=38)                                                                                                    cATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCa                                   >  2:1512817/1‑140 (MQ=255)                                                                                                            aatcaatGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTACTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGc                           <  2:145156/140‑1 (MQ=255)                                                                                                              tcaatGCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGAATCGGGCAATTGTGGTTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCaa                         >  2:792294/1‑140 (MQ=255)                                                                                                              tcaatGCCTGATGCGACGCTGTCGCGCCTGATCTGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTTCGCCAGGAGCGGCaa                         >  2:1125744/1‑140 (MQ=39)                                                                                                              tcaatGCCTGATGCGACGCTGTCGCGCCTGATCAGGGCTACAAACCGTGACCGACTCGCATCGGGCCATGGTGGGTTACGATGGCATCGCGTTAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGGCAGGATCGGCaa                         >  2:792341/1‑140 (MQ=38)                                                                                                                   gCCTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTGCTCGACTACGCCAGGATCGGCAAGCGTc                    >  2:1211981/1‑140 (MQ=255)                                                                                                                    ccTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCTAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCg                   <  1:39430/140‑1 (MQ=255)                                                                                                                    ccTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCg                   <  1:1559115/140‑1 (MQ=255)                                                                                                                    ccTGATGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCg                   <  1:1554303/140‑1 (MQ=255)                                                                                                                        aTGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGt               >  2:1673708/1‑140 (MQ=255)                                                                                                                        aTGCGACGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGt               <  2:991240/140‑1 (MQ=255)                                                                                                                              cGCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGcc         >  1:722082/1‑140 (MQ=255)                                                                                                                               gCTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGccc        >  1:884612/1‑140 (MQ=255)                                                                                                                                cTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCa       >  2:143137/1‑140 (MQ=255)                                                                                                                                cTGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGAAAATGTGGGTTACGAAGGCAACGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCa       >  2:791768/1‑140 (MQ=255)                                                                                                                                 tGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAg      <  1:1024962/140‑1 (MQ=255)                                                                                                                                 tGTCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAg      <  1:1024908/140‑1 (MQ=255)                                                                                                                                   tCGCGCCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGt    >  1:649290/1‑140 (MQ=255)                                                                                                                                     gcgcCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTg  <  2:829947/140‑1 (MQ=255)                                                                                                                                     gcgcCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTg  <  2:266914/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTG  >  NC_000913/4285229‑4285500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑