Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,082 A→C 7.8% intergenic (+480/+134) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,0820AC 7.8% 272.8 / 4.5 111intergenic (+480/+134)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base A (38/59);  new base C (2/9);  total (41/70)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACC  >  NC_000913/3269934‑3270229
                                                                                                                                                    |                                                                                                                                                   
catCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGTATAGTGTCGTGGACAGTCATTCATCGTTATGCCCCTCCAAATGCAAAATCCCGCCGag                                                                                                                                                    >  2:280364/1‑149 (MQ=11)
 atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGTGTCGTGGACAGTCATTCAACATTCTGCCCCGCCAAAAGCAAAAACCCGCCGCAg                                                                                                                                                   >  2:423100/1‑150 (MQ=11)
 atCTTTCTACCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAg                                                                                                                                                   >  2:487390/1‑150 (MQ=255)
 aacTTTCTGCCCATCCAAAAGTAAAAACCCGCCGAAGCGTGTTTATACGTAAAACAGGAGAAACTCACCGATAAGCCGCTTTCTTTTGGGGATAGTCTCGTGGCCAGTCATTCAACTTTATGCCCCTACAAAAGCACAAACCCGCCGAAg                                                                                                                                                   >  2:2571471/3‑150 (MQ=11)
         gCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTTCCCCTACAAAAGCAAAAACCCGCCCAAGCGGGtttt                                                                                                                                           >  2:2486633/1‑150 (MQ=255)
         gCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGTCAATCAAAATACTGCGCCACCAACAGCAAAAACCCGCCGAAGCCGGtttt                                                                                                                                           >  2:1226862/1‑150 (MQ=11)
          ccccTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt                                                                                                                                          <  2:1456258/150‑1 (MQ=11)
          ccccTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt                                                                                                                                          <  2:1701309/150‑1 (MQ=11)
           cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAAAGGTGAAACTGACCGATAAGCCGCTTTGTTTTGGGTATAGTGTCGTGGTCAGTCATTGATCTTTCTGGCCCTGCAAAAGAAAAAAAACGCGGGTGCGGGtttttt                                                                                                                                         >  1:1330648/1‑149 (MQ=11)
           cccTCCAAAAGTAAAAACCCGCCGAAGCGGGGTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGATAGTGTCGTGGTCACGCATTGATCTTTCTGCCCCGACAAAAGCAAAAACCCCCCCAAGCGGGgttttg                                                                                                                                         >  1:1221534/1‑149 (MQ=11)
            ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                                        <  1:1457966/150‑1 (MQ=37)
             cTCCACAAGTAACAACCCGCCGCAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                                       <  2:2747494/150‑1 (MQ=255)
             cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                                       >  1:58135/1‑150 (MQ=37)
              tCCAAAAGTAAAAACCCGCCGAAGCTGTTTTTTACTTAAAACAGGTGAAACTGACCTATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgctt                                                                                                                                      <  1:742762/150‑5 (MQ=11)
              tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                                      <  1:738973/150‑1 (MQ=17)
              tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGACACTGACCGATACGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                                      <  1:1188759/150‑1 (MQ=255)
              tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCGTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCAGCCGAAGCGGGTTTTTAAGt                                                                                                                                      >  2:172894/1‑150 (MQ=255)
                  caaGTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                                                  <  2:2201085/149‑1 (MQ=38)
                  aaaGTATAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                                                  <  2:358380/150‑1 (MQ=38)
                  aaaGTAAAACCCCGCCGTAGCGGGTTTTTACGTAAATCAGGTGAAAGTGACCGATAAGCCGCTTTCTTTGGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                                                  <  1:1813003/150‑1 (MQ=11)
                  aaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                                                  <  1:1131165/150‑1 (MQ=38)
                      tAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                                         <  2:497623/139‑1 (MQ=11)
                      tAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                                         >  1:497623/1‑139 (MQ=11)
                      tAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACAGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg                                                                                                                              >  2:1069165/1‑150 (MQ=38)
                          aaCCCGCCGAAGCGTGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa                                                                                                                          <  2:1979447/150‑1 (MQ=37)
                             ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCATTCATCTTTCTGCCCCACCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGATACt                                                                                                                       >  1:1821914/1‑150 (MQ=255)
                             ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                                                                       >  2:1595771/1‑150 (MQ=17)
                             ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                                                                                                                       >  2:630487/1‑150 (MQ=21)
                               gccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCACCAAAACCAAAAAAACGCCGAACCGCGGTTTTACGTAAAACAGGTGaaaagga                                                                                                                     >  1:2635691/1‑146 (MQ=11)
                               gccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACAGa                                                                                                                     >  1:1746082/1‑150 (MQ=255)
                                          tttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCg                                                                                                          <  1:1356250/150‑1 (MQ=37)
                                           ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                                                         <  1:1733100/150‑1 (MQ=38)
                                            tttACGTACAACAGGTGAAACTGACCGATAAGCCGCATTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGct                                                                                                        <  2:193158/150‑1 (MQ=255)
                                             ttACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt                                                                                                       <  2:682451/150‑1 (MQ=21)
                                             ttACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt                                                                                                       <  2:527293/150‑1 (MQ=255)
                                             ttACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt                                                                                                       <  2:682487/150‑1 (MQ=37)
                                             ttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt                                                                                                       >  1:1681682/1‑150 (MQ=39)
                                             ttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt                                                                                                       >  2:1808208/1‑150 (MQ=39)
                                                  tataaCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGACCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                                  <  2:416556/147‑1 (MQ=255)
                                                  tacatcAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                                  <  2:1103917/145‑1 (MQ=2)
                                                  tAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCAATTAATACAACTTGATGCCCCGCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGAAAAAAAGGTGAAAATTACCGAAAAGCCGcgttcttt                                                                                                  >  2:1416053/1‑150 (MQ=11)
                                                  tAAAACAGCTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACCAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctgtcttt                                                                                                  <  2:1017961/150‑1 (MQ=255)
                                                   taaaCAGGTGAATCGGACCGATAAGCCGCTTTCTTTTGGGTAGAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                 <  1:1658943/149‑1 (MQ=255)
                                                   acatccggTGACACTGCCCGATCCGCCGGTGTCTTTTGGGTCTCGTGTCGTGGACCGTCATTCATCTTTCTGCCCCGCCACAAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                 <  2:1883978/144‑1 (MQ=11)
                                                   aacaCAGGTGAAACTGACCGATCCGCCGCTTTCTTTGGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                 <  1:1658961/147‑1 (MQ=255)
                                                   aaatCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                 <  1:1812641/150‑1 (MQ=18)
                                                   aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                 <  1:1938581/150‑1 (MQ=40)
                                                   aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                 <  1:16908/150‑1 (MQ=40)
                                                   aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                 <  1:226181/150‑1 (MQ=40)
                                                    acaCAGGATAAACTGACCGATACGCCGCCTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                <  2:2445138/148‑1 (MQ=255)
                                                     aaCATGTTACACTGACCGAGAATCCGCTTTCTTTTGGTTAGTGTGTCGTGGACAGTCATTCTTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                               <  1:33691/150‑1 (MQ=11)
                                                     aaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                               <  1:1063019/150‑1 (MQ=38)
                                                     aaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                               <  1:243407/150‑1 (MQ=38)
                                                        aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCAATAATCATTATGCCCCACCCAAACCAAAAAACCCCCCAAGCGGGTGTATGAGAAAACCAGGGGGAACGTAACGATAAGCCGCTTTGTTTTGGGtg                                                                                            >  1:1267449/1‑149 (MQ=2)
                                                         ggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtat                                                                                           <  1:2066780/150‑1 (MQ=37)
                                                           tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGGTCTTTgggggagag                                                                                         >  2:2531049/1‑145 (MQ=11)
                                                            gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGc                                                                                        <  2:464727/150‑1 (MQ=35)
                                                                 tgtccGTTAAGCCGCTTTCTTTTTTGTATCGTGTCGAGGACAGACATTCTTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTCAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                   <  2:286472/147‑1 (MQ=11)
                                                                 tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                   <  2:932673/150‑1 (MQ=37)
                                                                   aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                                                         <  1:2219223/110‑1 (MQ=35)
                                                                             cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                                                                                                                           <  1:934021/98‑1 (MQ=37)
                                                                               ctttctttTGTGGTTTGTGTCGTGGACAGTCATTCAACTTACTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcat                                                                     <  1:2233161/150‑1 (MQ=11)
                                                                               ctttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcat                                                                     <  1:1962385/150‑1 (MQ=37)
                                                                                    ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTc                                                                <  1:85586/150‑1 (MQ=37)
                                                                                     tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTATGCCCCACCAAAAGCAAAAACCCGCCCAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCCCGTTATTTTGGGTATAGCGTCGTGGACAGTCCTTAATCattat                                                               >  1:542670/1‑148 (MQ=11)
                                                                                       tGGGTATCGTGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGc                                                             <  2:1307355/150‑1 (MQ=255)
                                                                                       tGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGc                                                             <  2:1666186/150‑1 (MQ=37)
                                                                                         ggtttcgtgtCGTGGACAGGCCTTCCTCTTTCTGCCCCTCCACAAGCACAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                                                           <  1:2708632/144‑1 (MQ=11)
                                                                                         ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                                                           <  1:2489480/150‑1 (MQ=37)
                                                                                         ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCAACTTTCTGccc                                                           >  2:1378741/1‑150 (MQ=12)
                                                                                                tgtCGTGGGCAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAACCAGGTGAAACTGCCCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGCCAGGCCTTCATCTGTCGGCCCCGCCaaa                                                    >  1:961081/1‑150 (MQ=11)
                                                                                                tgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTGTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCCTTCATCTTTATGCCCCTCCaaa                                                    >  2:1276108/1‑150 (MQ=255)
                                                                                                 gtCGTGGACAGTCTGTCTTCTTTCTGCCACTCCAAAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                   <  2:694912/150‑1 (MQ=11)
                                                                                                 gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                   <  2:1815469/150‑1 (MQ=37)
                                                                                                                tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCAACTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGa                                    >  2:1350504/1‑150 (MQ=255)
                                                                                                                  atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcttcgg                                  <  1:1808208/150‑6 (MQ=37)
                                                                                                                  atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAg                                  >  1:2135981/1‑150 (MQ=37)
                                                                                                                  atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATGCAACATGCTGCCCCGACAAAAGCAAAAACCCGCCGCAg                                  >  1:2135866/1‑150 (MQ=11)
                                                                                                                   tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                 >  1:373674/1‑150 (MQ=37)
                                                                                                                   tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                 >  1:639587/1‑150 (MQ=37)
                                                                                                                   tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                 >  1:639608/1‑150 (MQ=37)
                                                                                                                   tCTTTCTGCCCCTACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                 >  1:2279817/1‑150 (MQ=37)
                                                                                                                          gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                          >  2:519755/1‑150 (MQ=37)
                                                                                                                            cccTCCAATAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGTCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGGCGAAGCGGGTTTTTa                        <  1:2078438/150‑1 (MQ=255)
                                                                                                                            cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                        <  1:1565943/150‑1 (MQ=37)
                                                                                                                            cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                        >  1:354105/1‑150 (MQ=37)
                                                                                                                            cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                        >  1:354162/1‑150 (MQ=37)
                                                                                                                            cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                        >  1:683172/1‑150 (MQ=37)
                                                                                                                            cccTCAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                        <  1:2578510/150‑1 (MQ=37)
                                                                                                                               tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCAACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                     >  2:91283/1‑150 (MQ=255)
                                                                                                                                   acaGCAAACACCCGCCGACGCGGGTTTTTACTTAAACCAGGTGAAACTGACCGATAAGCCGATTTCTTTTGGGTATAGCGTCGTTGACAGTCATTCATCTTTCTGCCCCTCCCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                 <  1:1401970/148‑1 (MQ=11)
                                                                                                                                      tcttaaaCCCGCCGAAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg              <  2:15102/146‑1 (MQ=255)
                                                                                                                                      gCACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg              <  2:2507591/150‑1 (MQ=17)
                                                                                                                                       ctcaaaCCCGCCGCACCGGGTTATTACGTACACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg             <  2:1363698/147‑1 (MQ=11)
                                                                                                                                       cAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg             <  2:679759/150‑1 (MQ=38)
                                                                                                                                          aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa          >  1:2712734/1‑150 (MQ=37)
                                                                                                                                              ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt      >  1:1603669/1‑150 (MQ=37)
                                                                                                                                              ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt      >  2:630603/1‑150 (MQ=17)
                                                                                                                                                  cgAAGCGGGTTTTTACGTAAACCAGGTGACACTGAGCGATTTGCCGCTTTCTTTTGGGTATCGAGTCGTTGACAGTCATTCATCTTTCTGCCGCTGCTATTGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAcc  <  2:1821914/150‑1 (MQ=11)
                                                                                                                                                  cgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAcc  <  1:1595771/150‑1 (MQ=17)
                                                                                                                                                    |                                                                                                                                                   
CATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACC  >  NC_000913/3269934‑3270229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: