Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,480 A→T 7.2% intergenic (+35/+52) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,4800AT 7.2% 421.9 / 16.4 150intergenic (+35/+52)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base A (43/86);  new base T (6/5);  total (59/91)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 159 total aligned reads displayed.

AACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTT  >  NC_000913/3959332‑3959623
                                                                                                                                                    |                                                                                                                                               
aaCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcac                                                                                                                                                >  2:653782/1‑150 (MQ=255)
aaCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcac                                                                                                                                                >  2:653841/1‑150 (MQ=255)
 aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                               <  1:1923357/150‑1 (MQ=255)
 aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                               <  1:823172/150‑1 (MQ=255)
 aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                               <  2:1303171/150‑1 (MQ=255)
 aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCcaca                                                                                                                                               >  1:1330038/1‑150 (MQ=255)
   gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagg                                                                                                                                             <  2:2344687/150‑1 (MQ=255)
       aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggaga                                                                                                                                         <  1:160409/150‑1 (MQ=255)
       aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggaga                                                                                                                                         <  1:315543/150‑1 (MQ=255)
       aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggaga                                                                                                                                         <  1:883197/150‑1 (MQ=255)
       aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCGCCCCTCTCCCACcgggagg                                                                                                                                         >  2:2262538/1‑149 (MQ=255)
         cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTACGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagg                                                                                                                                       <  2:2510919/150‑1 (MQ=255)
         cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagg                                                                                                                                       >  2:1847268/1‑150 (MQ=255)
         cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagg                                                                                                                                       >  2:1395088/1‑150 (MQ=255)
         cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagg                                                                                                                                       <  1:754580/150‑1 (MQ=255)
          tGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagaggg                                                                                                                                      <  2:1763163/150‑1 (MQ=255)
           gcttGATGTGAACGTAGCGATTCGCAGCCATGCGATTGATCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGTTATGTAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                                     <  1:1438610/147‑1 (MQ=38)
           gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGTCTATATGACAGATATGTAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggtgaggga                                                                                                                                     <  1:1438575/150‑1 (MQ=255)
           gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                                     <  1:1845929/150‑1 (MQ=255)
           gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCACCCACacggagaggga                                                                                                                                     >  2:1458880/1‑150 (MQ=255)
              tGACGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagggtcgg                                                                                                                                  <  2:961845/150‑5 (MQ=255)
              tGACGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagggtcgg                                                                                                                                  <  2:961734/150‑5 (MQ=255)
                  gtgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCGCAACCCCAGGGCGCCGGGGaaaaaa                                                                                                                              >  2:965640/1‑148 (MQ=255)
                   tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAacac                                                                                                                             >  2:2730543/1‑150 (MQ=255)
                   tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAacac                                                                                                                             >  2:1332671/1‑150 (MQ=255)
                   tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCCCCCCGACCCACAACCACAGGGAGAGGGAGGAAacac                                                                                                                             >  2:1203683/1‑150 (MQ=255)
                    gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCCCAGGGAGAGGGAGAAAacaca                                                                                                                            >  1:2056287/1‑149 (MQ=255)
                    gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCACACCCCCAGGGGGGGGGTGAAAaaacg                                                                                                                            >  1:75351/1‑149 (MQ=255)
                    gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCTCCCACAGGGAGTGGGTGAAAacacg                                                                                                                            >  1:1592051/1‑149 (MQ=255)
                    gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCACCCACTCCCCCCGGGCGAGGGGGAAAacacg                                                                                                                            >  1:1645916/1‑149 (MQ=255)
                    gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCACCCACTACCACAGGGAGTGGGAGGAAaccca                                                                                                                            >  1:12481/1‑147 (MQ=255)
                      aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCa                                                                                                                          <  2:996767/150‑1 (MQ=255)
                      aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCa                                                                                                                          <  2:931482/150‑1 (MQ=255)
                      aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCa                                                                                                                          <  2:2121270/150‑1 (MQ=255)
                      aCGAAGCGAGTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCa                                                                                                                          <  2:2399784/150‑1 (MQ=255)
                         aaGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAgg                                                                                                                       <  2:201040/150‑1 (MQ=255)
                         aaGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAgg                                                                                                                       <  2:2096015/150‑1 (MQ=255)
                         aaGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAgg                                                                                                                       <  2:201002/150‑1 (MQ=255)
                          aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAACCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                                      <  2:2606918/150‑1 (MQ=255)
                          aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                                      <  1:2462833/150‑1 (MQ=255)
                          aGCGATTCGCAGCCATGCGATTGAGCAGGGAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                                      <  2:1401742/150‑1 (MQ=255)
                             gATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACACTCTCCCACAGGGAGAGGGAGAAAACACTAAAGGCCtt                                                                                                                   >  2:423344/1‑150 (MQ=255)
                                tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGtctc                                                                                                                >  1:1334677/1‑150 (MQ=255)
                                tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCAATCCCCCCGGGCGAGGGGGAAAACACGCAAGGccctatc                                                                                                                >  2:284802/1‑145 (MQ=255)
                                tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCACTACCACAGGGTGAGGGGGAGAACACACAAGGccgtatc                                                                                                                >  2:1487433/1‑145 (MQ=255)
                                tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGGCCACACCCCCACCCACAACCACCCGGTGGGGGAGAAAAAAAACAAGGCCAtcga                                                                                                                >  1:2135172/1‑148 (MQ=255)
                                tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCACCCACACCCCCCCGGAGAGGGAGAAAAAACACAAGGccgtatc                                                                                                                >  2:284835/1‑145 (MQ=255)
                                 cGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACACCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTcc                                                                                                               >  1:1704820/1‑150 (MQ=255)
                                    aGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTgg                                                                                                            <  1:1857007/150‑1 (MQ=255)
                                     gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                           <  1:2652488/150‑1 (MQ=255)
                                     gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                           <  1:1575933/150‑1 (MQ=255)
                                          gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGGTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                                      <  1:1922148/150‑1 (MQ=255)
                                            gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGcc                                                                                                    <  2:1538755/150‑1 (MQ=255)
                                             aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                   <  1:2657037/150‑1 (MQ=255)
                                                gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAAAACTCAAGGCCTTCTCCTGGAGAAGGCCGTGc                                                                                                >  1:2036616/1‑150 (MQ=255)
                                                gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCAGGAGAAGGCCTTga                                                                                                <  2:2588245/150‑2 (MQ=255)
                                                gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACACAAGGCCATCTCCTGGAGAAGGCCTTGc                                                                                                >  1:656404/1‑150 (MQ=255)
                                                   cAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                                                                                             <  2:35140/150‑1 (MQ=255)
                                                    aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGTCCTTCTCCTGGAGAAGGCCTTGCTAtt                                                                                            <  2:1866319/150‑1 (MQ=255)
                                                    aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCGACCCACACCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCGTGCTaat                                                                                            >  2:2230646/1‑148 (MQ=39)
                                                        aggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTACCACACGGAGAGGGAGAAAACACAAAAGGCCATCTCCGGGAGTAGGCCTTGGGATTAttt                                                                                        >  1:2619380/1‑150 (MQ=37)
                                                         ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg                                                                                       <  1:653782/150‑1 (MQ=255)
                                                                      gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTACGTGCGCGCTGATGCCCTCACCCCGACCCACACCCACACGGCCAGGGCCCAAACACACAAGGCCGTCTCCAGGCGAAGGCCATGCTAAAAGTTGCGGTACaccacca                                                                          >  2:2618708/1‑149 (MQ=255)
                                                                      gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCGACCCCCAACCACAGGGAGAGGGTGAAAACACTCAAGGCCATCTCCTGGCGGAGGCCGGGCGATTAGTTGAGGGACAGCAcca                                                                          >  1:834379/1‑149 (MQ=255)
                                                                       cgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCCCACACCCACAGGGAGAGGGGGGAAAAAAACAAGGCCGACACCAGGTGAAGGCCGTTGTATTAGTTTGGGTTCAGAAccat                                                                         >  1:867131/1‑148 (MQ=19)
                                                                          cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                                      <  1:215794/150‑1 (MQ=255)
                                                                                 aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCACCCACACCCACAGGGGGAGGGGGAAAAAACAACAGGCCATATACGGGTGGAGGCCATGGTTTTATTTGGGGTACAGCACAATAAAGATTTGa                                                               >  1:1782027/1‑150 (MQ=255)
                                                                                     atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                           <  1:609819/150‑1 (MQ=255)
                                                                                        tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa                                                        <  1:1017381/150‑1 (MQ=255)
                                                                                        tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa                                                        <  1:1017447/150‑1 (MQ=255)
                                                                                                    gtGTTGCGGGTTCAGTGCGCGCGGCGGCCCGCACCCCGACCCTCTCCCACAGGGGGAGGGAGAAAACACGCAAGGCCGGCTCCGGGAGGCGGCCGGGCGCATAGGGGCGGGACAGCACCGTCAGGAGGTGATCTCCGTCCGGGGGGTGCa                                            >  2:1672860/2‑150 (MQ=255)
                                                                                                    cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACTAAAGGCCTTCTCCTGGAGAAGGCCTTGCAATTAGTTGCGGTACAGCAACTTAATGATGTGATATCCGAACAGGGTGTGCa                                            >  2:2277850/1‑150 (MQ=38)
                                                                                                     tGTTGCGGGTTAAGTGCGCGCTGATGCCCGAACCCCCACCCACAACCACCCGGAGAGGGAGAAAAAAAACAAAGCCATAAACAGGAGAAGGCCGTGCTATTATTTTCGGTACACCACCAAAAATAATTGATATACGAAAAGGGTGTGCAg                                           >  2:786806/1‑150 (MQ=255)
                                                                                                     tGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACACGGCGAGGGGGAAAAAACACAAGGCCATCTCCAGGAGAAGGCCATGGTAATAGTTGCGGTACCGCACCATAATGAAGTGATATCCGAACGGGGGGTGCac                                           >  2:1882825/1‑149 (MQ=255)
                                                                                                     tGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCCACCCACAACCACAGGGGGAGGGGGAAAAAACTCAAGGCCATAAACGGGAGAAGGCCGTGGTATTAGTTTCGGTACACCAACATAATGTTGTGATATCCGAACTGGGGGTGCAg                                           >  2:534153/1‑150 (MQ=255)
                                                                                                     tGTTGCGGGTTAAGTGCGCGCTGAGGCCCACAACCCCAACCACAAACACAGGGAGAGGGAGAAAAAACAAAAAGCCATAAACAGGAGAAAGCCTTGCTAATATTTGCGGTACAGCACCATAATGTATTGATATACGAACAGGgttttcac                                           >  2:1085201/1‑144 (MQ=255)
                                                                                                            ggTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg                                    <  1:1656829/150‑1 (MQ=255)
                                                                                                                aaGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCg                                <  1:1134473/150‑1 (MQ=255)
                                                                                                                      gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                          <  1:1015218/150‑1 (MQ=255)
                                                                                                                      gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                          <  1:197403/150‑1 (MQ=255)
                                                                                                                      gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                          <  2:1385580/150‑1 (MQ=255)
                                                                                                                      gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                          <  1:767616/150‑1 (MQ=255)
                                                                                                                      gcgcTGAGGCCCTCCCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                          <  1:71698/150‑1 (MQ=255)
                                                                                                                          tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATGCGAAGTGGGTGTGGAGCGGGCCGGTCGGCTCCAGTAc                      >  2:1094117/1‑150 (MQ=255)
                                                                                                                          tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATGCGAACGGGGGGTGCAGCGGGCCGGTCGGCTCCAGTAc                      >  2:1425211/1‑150 (MQ=255)
                                                                                                                          tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAc                      >  2:893842/1‑150 (MQ=255)
                                                                                                                           gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                     <  1:2673144/150‑1 (MQ=255)
                                                                                                                           gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                     <  1:1203890/150‑1 (MQ=255)
                                                                                                                           gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTAGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                     <  1:301076/150‑1 (MQ=255)
                                                                                                                                ccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACa                <  1:1839615/150‑1 (MQ=255)
                                                                                                                                   cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga             <  1:2618009/150‑1 (MQ=255)
                                                                                                                                   cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga             <  1:1045346/150‑1 (MQ=255)
                                                                                                                                   cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga             <  1:2690503/150‑1 (MQ=255)
                                                                                                                                      cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa          >  2:695935/1‑150 (MQ=255)
                                                                                                                                        cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa        <  1:224315/150‑1 (MQ=255)
                                                                                                                                        cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa        <  1:224319/150‑1 (MQ=255)
                                                                                                                                         ctcccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAc       >  2:2693780/3‑150 (MQ=255)
                                                                                                                                         ctcccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAacac       >  2:2693663/3‑150 (MQ=255)
                                                                                                                                           cccTCTCCCACAGGGAGAGGGAGAACACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa     <  2:2589103/150‑1 (MQ=255)
                                                                                                                                            ccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc    >  2:1000282/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
AACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTT  >  NC_000913/3959332‑3959623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: