Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,515 T→A 7.8% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,5150TA 7.8% 421.9 / 10.9 144intergenic (+70/+17)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (47/78);  new base A (10/4);  total (62/82)
Fisher's exact test for biased strand distribution p-value = 2.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

CAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCT  >  NC_000913/3959367‑3959664
                                                                                                                                                    |                                                                                                                                                      
cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                                                       <  1:1889246/150‑1 (MQ=255)
cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                                                       <  1:2021032/150‑1 (MQ=255)
  gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                                                                     <  1:2652488/150‑1 (MQ=255)
  gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                                                                     <  1:1575933/150‑1 (MQ=255)
         gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGcc                                                                                                                                              <  2:1538755/150‑1 (MQ=255)
         gATTGAGCAGGGAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGcc                                                                                                                                              <  2:2485654/150‑1 (MQ=255)
          aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                                                            <  1:2656714/150‑1 (MQ=255)
            tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                                                          <  1:304724/150‑1 (MQ=255)
             gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAAAACTCAAGGCCTTCTCCTGGAGAAGGCCGTGc                                                                                                                                         >  1:2036616/1‑150 (MQ=255)
                cAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                                                                                                                                      <  2:35140/150‑1 (MQ=255)
                 aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCGACCCACACCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCGTGCTaat                                                                                                                                     >  2:2230646/1‑148 (MQ=39)
                     aggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTACCACACGGAGAGGGAGAAAACACAAAAGGCCATCTCCGGGAGTAGGCCTTGGGATTAttt                                                                                                                                 >  1:2619380/1‑150 (MQ=37)
                      ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg                                                                                                                                <  1:653782/150‑1 (MQ=255)
                      ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATCAGTTg                                                                                                                                <  1:653841/150‑1 (MQ=255)
                                tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCACCCACTCCCCCCGGGAGAGGGGGAAAAAAAACAAAGCCAAATACAGGAGAAGGCCATGCGGTTAGTTTAGGTACAGCa                                                                                                                      >  1:761335/1‑150 (MQ=255)
                                   gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCGACCCCCAACCACAGGGAGAGGGTGAAAACACTCAAGGCCATCTCCTGGCGGAGGCCGGGCGATTAGTTGAGGGACAGCAcca                                                                                                                   >  1:834379/1‑149 (MQ=255)
                                    cgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCCCACACCCACAGGGAGAGGGGGGAAAAAAACAAGGCCGACACCAGGTGAAGGCCGTTGTATTAGTTTGGGTTCAGAAccat                                                                                                                  >  1:867131/1‑148 (MQ=19)
                                                  atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                                                    <  1:609819/150‑1 (MQ=255)
                                                  atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                                                    <  1:39646/150‑1 (MQ=255)
                                                     tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa                                                                                                 <  1:1017381/150‑1 (MQ=255)
                                                     tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGa                                                                                                 <  1:1017422/150‑1 (MQ=255)
                                                          cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                            <  1:1130829/150‑1 (MQ=255)
                                                                 gtGTTGCGGGTTCAGTGCGCGCGGCGGCCCGCACCCCGACCCTCTCCCACAGGGGGAGGGAGAAAACACGCAAGGCCGGCTCCGGGAGGCGGCCGGGCGCATAGGGGCGGGACAGCACCGTCAGGAGGTGATCTCCGTCCGGGGGGTGCa                                                                                     >  2:1672860/2‑150 (MQ=255)
                                                                 cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACTAAAGGCCTTCTCCTGGAGAAGGCCTTGCAATTAGTTGCGGTACAGCAACTTAATGATGTGATATCCGAACAGGGTGTGCa                                                                                     >  2:2277850/1‑150 (MQ=38)
                                                                  tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTACCACAGGGAGAGGGAGAAAACACTCAATGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAg                                                                                    >  2:2752976/1‑150 (MQ=255)
                                                                  tGTTGCGGGTTAAGTGCGCGCTGATGCCCGAACCCCCACCCACAACCACCCGGAGAGGGAGAAAAAAAACAAAGCCATAAACAGGAGAAGGCCGTGCTATTATTTTCGGTACACCACCAAAAATAATTGATATACGAAAAGGGTGTGCAg                                                                                    >  2:786806/1‑150 (MQ=255)
                                                                  tGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACACGGCGAGGGGGAAAAAACACAAGGCCATCTCCAGGAGAAGGCCATGGTAATAGTTGCGGTACCGCACCATAATGAAGTGATATCCGAACGGGGGGTGCac                                                                                    >  2:1882825/1‑149 (MQ=255)
                                                                  tGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCAAACGGAGAGTGGGAAAAAAAAAAAGGCCATCGCCAGGGTCAGGCCGTTGCAATATTTTCGGGAACGCACCATAATGATGTGGTATAAGAAATGGgtgttgag                                                                                    >  2:6002/1‑146 (MQ=255)
                                                                  tGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCCACCCTCACCCACAGGGAGAGGGAGAAAACAAACAAGGCCGACTCCAGGAGAAGGCCGTGCTATTATTTTCGGTAAAGCACCATAAAGATGTGATATACGAACTGGGGGTGCAg                                                                                    >  2:1882823/1‑150 (MQ=25)
                                                                  tGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCCACCCACAACCACAGGGGGAGGGGGAAAAAACTCAAGGCCATAAACGGGAGAAGGCCGTGGTATTAGTTTCGGTACACCAACATAATGTTGTGATATCCGAACTGGGGGTGCAg                                                                                    >  2:534153/1‑150 (MQ=255)
                                                                  tGTTGCGGGTTAAGTGCGCGCTGAGGCCCACAACCCCAACCACAAACACAGGGAGAGGGAGAAAAAACAAAAAGCCATAAACAGGAGAAAGCCTTGCTAATATTTGCGGTACAGCACCATAATGTATTGATATACGAACAGGgttttcac                                                                                    >  2:1085201/1‑144 (MQ=255)
                                                                    ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                                  <  2:1432792/150‑1 (MQ=255)
                                                                         ggTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg                                                                             <  1:1656829/150‑1 (MQ=255)
                                                                             aaGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCg                                                                         <  1:1134473/150‑1 (MQ=255)
                                                                                 gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGTGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCTGCTc                                                                     <  2:1167751/150‑1 (MQ=255)
                                                                                 gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                                                     <  2:2696755/150‑1 (MQ=255)
                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                   <  1:1015218/150‑1 (MQ=255)
                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                   <  1:767616/150‑1 (MQ=255)
                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                   <  1:197403/150‑1 (MQ=255)
                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                   <  2:510007/150‑1 (MQ=255)
                                                                                   gcgcTGAGGCCCTCCCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                   <  1:71698/150‑1 (MQ=255)
                                                                                       tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGTGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAc                                                               >  2:2538491/1‑150 (MQ=255)
                                                                                       tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATGCGAACGGGGGGTGCAGCGGGCCGGTCGGCTCCAGTAc                                                               >  2:1425211/1‑150 (MQ=255)
                                                                                       tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAc                                                               >  2:893842/1‑150 (MQ=255)
                                                                                       tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAc                                                               >  2:2213124/1‑150 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                              <  1:2673144/150‑1 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                              <  2:316976/150‑1 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                              <  1:138662/150‑1 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                              <  1:1203890/150‑1 (MQ=255)
                                                                                        gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTAGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                              <  1:301076/150‑1 (MQ=255)
                                                                                         aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg                                                             >  2:1777525/1‑150 (MQ=255)
                                                                                             ccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACa                                                         <  1:1839636/150‑1 (MQ=255)
                                                                                             ccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACa                                                         <  1:1839615/150‑1 (MQ=255)
                                                                                                cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                                                      <  1:2618009/150‑1 (MQ=255)
                                                                                                   cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                                                   >  2:695935/1‑150 (MQ=255)
                                                                                                     cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                                 <  1:224319/150‑1 (MQ=255)
                                                                                                     cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                                 <  1:224315/150‑1 (MQ=255)
                                                                                                     cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                                 <  2:88017/150‑1 (MQ=255)
                                                                                                      ctcccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAc                                                >  2:2645353/3‑150 (MQ=255)
                                                                                                      ctcccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAc                                                >  2:152346/3‑150 (MQ=255)
                                                                                                      ctcccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAacac                                                >  2:2693663/3‑150 (MQ=255)
                                                                                                        cccTCTCCCACAGGGAGAGGGAGAACACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa                                              <  2:2589103/150‑1 (MQ=255)
                                                                                                        cccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa                                              <  1:1761861/150‑1 (MQ=255)
                                                                                                        cccTCACCCACAGGGAGAGGGAGAAAACCCTCACGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa                                              <  2:1399670/150‑1 (MQ=255)
                                                                                                         ccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc                                             >  2:1000282/1‑150 (MQ=255)
                                                                                                         ccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACGGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc                                             >  2:2602288/1‑150 (MQ=255)
                                                                                                           tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTGGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCCGTACCGGACAAGAGACAACCACtt                                           >  1:2229045/1‑150 (MQ=255)
                                                                                                                     ggagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGc                                 <  2:75351/150‑1 (MQ=255)
                                                                                                                        gagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCgg                              <  2:1330038/150‑1 (MQ=255)
                                                                                                                        gagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCgg                              <  2:1577080/150‑1 (MQ=255)
                                                                                                                              gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt                        >  1:1885415/1‑150 (MQ=255)
                                                                                                                               aaaaCCCTCACGGCCTGCTCCTGGAGAAGGCCTTGCTATTCGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                       <  1:566476/150‑1 (MQ=255)
                                                                                                                               aaaaCACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                       <  1:1228285/150‑1 (MQ=255)
                                                                                                                               aaaaCACTCACGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                       <  1:2666018/150‑1 (MQ=255)
                                                                                                                               aaaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                       >  1:489080/1‑150 (MQ=255)
                                                                                                                               aaaaCACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTCGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                       <  1:2066503/150‑1 (MQ=255)
                                                                                                                                        caGGCCGTCTCCTGGAGAAGGCCTTGCTATTCGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTgg              <  1:1503354/149‑1 (MQ=255)
                                                                                                                                        aaGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATCTCCGACCTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGACAACCACTTTATCGAACGCCGGACCCATCTGACCCTgg              >  1:763088/1‑150 (MQ=255)
                                                                                                                                        aaGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTgg              >  2:2671308/1‑150 (MQ=255)
                                                                                                                                        aaGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTgg              >  1:2406720/1‑150 (MQ=255)
                                                                                                                                        aaGGCCTTCTCCTGGAGAAGGCCTTGATAGAAGTTGCGGTAAAGAACCAAAATGATGTTAAATACGTACTGGGTGTGAATCTGGACGGTCGTCACCAGTAACGGACAAAACAAAACCACAGAAACGAAATCAGGAACCAACGGACACTgg              >  2:1705902/1‑150 (MQ=255)
                                                                                                                                        aaGGCCGTCTCCGGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTGTATCGAACGCCGGAACCATCTGACCCTgg              >  2:1604173/1‑150 (MQ=255)
                                                                                                                                         aGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGc             <  1:1519874/150‑1 (MQ=255)
                                                                                                                                         aGGCCTTCTCCTGGAGAAGGCATTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGc             <  1:900071/150‑1 (MQ=255)
                                                                                                                                          ggCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCg            >  1:2095472/1‑150 (MQ=255)
                                                                                                                                          ggCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCg            >  1:2095432/1‑150 (MQ=255)
                                                                                                                                          ggCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCg            >  2:1283562/1‑150 (MQ=255)
                                                                                                                                             ctgctcCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGac         <  1:908015/147‑2 (MQ=255)
                                                                                                                                             cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa         >  1:1784735/1‑150 (MQ=255)
                                                                                                                                             cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa         >  1:1784771/1‑150 (MQ=255)
                                                                                                                                             cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa         >  1:1993751/1‑150 (MQ=255)
                                                                                                                                             cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa         <  1:1434235/150‑1 (MQ=255)
                                                                                                                                             cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa         <  2:1798281/150‑1 (MQ=255)
                                                                                                                                             cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa         >  1:1042118/1‑150 (MQ=255)
                                                                                                                                             cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCCAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa         <  1:701134/150‑1 (MQ=255)
                                                                                                                                             cTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACCGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGGACCGGACAAGAGAAAACCACTTTATCGAACGCCGGGACCTTCTGACCCTGGCGGaa         >  2:2624803/1‑150 (MQ=255)
                                                                                                                                             cTTCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa         <  2:1417181/150‑1 (MQ=255)
                                                                                                                                                    tGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCt  <  1:1780790/150‑1 (MQ=255)
                                                                                                                                                    tGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCt  >  1:1022606/1‑150 (MQ=255)
                                                                                                                                                    tGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCt  >  2:1307736/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCT  >  NC_000913/3959367‑3959664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: