BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,559,337	A→C	11.9%	F64V (TTT→GTT)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,559,337	0	A	C	11.9%	715.1 / 23.9	244	F64V (TTT→GTT)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base A (90/119); new base C (31/3); total (121/123)
Fisher's exact test for biased strand distribution p-value = 5.91e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTC  >  NC_000913/4559193‑4559486

   gagagaTAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATACCACCTGGGc                                                                                                                                               >  1:1677064/1‑150 (MQ=255)

    agagaTAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCa                                                                                                                                              <  2:914879/150‑1 (MQ=255)

    agagaTAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGACCAATAAAACCTGGGCa                                                                                                                                              <  2:2320011/150‑1 (MQ=255)

      agaTAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCACTCCCACCTGGGCaga                                                                                                                                            >  1:2162944/1‑150 (MQ=255)

      agaTAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCCCACCTGGGCaga                                                                                                                                            >  1:294055/1‑150 (MQ=255)

      agaTAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCCCACCTGGGCaga                                                                                                                                            >  1:2095052/1‑150 (MQ=255)

        aTAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCCCACCTGGGCAGAgg                                                                                                                                          >  1:443953/1‑150 (MQ=255)

        aTAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCACACCTGGGCAGAgg                                                                                                                                          >  1:2311878/1‑150 (MQ=255)

             gTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTg                                                                                                                                     <  1:953962/150‑1 (MQ=255)

              tCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGc                                                                                                                                    >  2:601096/1‑150 (MQ=255)

                ggTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGccc                                                                                                                                  >  2:1437461/1‑150 (MQ=255)

                   gCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACt                                                                                                                               <  2:2331786/150‑1 (MQ=255)

                      cagcagACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCCCACCTGGGCAGAGGATCTGCCCACTgag                                                                                                                            >  1:1246060/1‑150 (MQ=255)

                              caaccaCTGACGTGACGCCCTCTTCAGTCAGGCGACTTAGAGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGACAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACaa                                                                                                                    >  1:1499362/1‑150 (MQ=255)

                              caaccaCTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACaa                                                                                                                    >  2:2669572/1‑150 (MQ=255)

                              caaccaCTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACaa                                                                                                                    >  2:621503/1‑150 (MQ=255)

                                   aCTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGTCCTGCTTCGCCGCCACCGCCTATCTTTTGGACGTGTTGATCAATAAAACCTGGGGAGAGGATCTGCCCACTGAGATCGACAACCGTg                                                                                                               <  2:2401017/150‑1 (MQ=255)

                                   aCTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTg                                                                                                               <  2:208518/150‑1 (MQ=255)

                                   aCTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTg                                                                                                               <  2:291188/150‑1 (MQ=255)

                                   aCTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTg                                                                                                               <  2:1675497/150‑1 (MQ=255)

                                       acgtgacgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCCCACCTGGGCAGAGGATCTGCCCACGGAGATCGACAACCGTGCAGt                                                                                                           >  1:1750656/1‑150 (MQ=255)

                                       acgtgacgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCACACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGt                                                                                                           >  1:1520177/1‑150 (MQ=255)

                                       acgtgacgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGAAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGt                                                                                                           >  1:2181760/1‑150 (MQ=255)

                                         gtgacgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTc                                                                                                         <  2:2725878/150‑1 (MQ=255)

                                         gtgacgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTc                                                                                                         <  2:1052285/150‑1 (MQ=255)

                                          tgacgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCg                                                                                                        <  2:917544/150‑1 (MQ=255)

                                          tgacgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCg                                                                                                        >  2:1977146/1‑150 (MQ=255)

                                          tgacgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCg                                                                                                        >  2:2122825/1‑150 (MQ=255)

                                          tgacgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCg                                                                                                        >  2:2570097/1‑150 (MQ=255)

                                            acgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCCCTCAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGt                                                                                                      >  1:2249995/1‑150 (MQ=255)

                                            acgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGt                                                                                                      >  1:2647631/1‑150 (MQ=255)

                                            acgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGt                                                                                                      >  2:1526826/1‑150 (MQ=255)

                                            acgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGt                                                                                                      >  2:2429377/1‑150 (MQ=255)

                                            acgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGt                                                                                                      <  1:555415/150‑1 (MQ=255)

                                            acgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGt                                                                                                      <  1:1061857/150‑1 (MQ=255)

                                            acgCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGt                                                                                                      <  1:247355/150‑1 (MQ=255)

                                                   cTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGt                                                                                               <  1:909210/150‑1 (MQ=255)

                                                       cGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAAGACCACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGATCGGTACAATGTCAGa                                                                                           >  1:1062481/1‑150 (MQ=255)

                                                            ggCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGa                                                                                      <  1:2289576/150‑1 (MQ=255)

                                                            ggCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGa                                                                                      >  2:1795755/1‑150 (MQ=255)

                                                            ggCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGa                                                                                      <  1:1130718/150‑1 (MQ=255)

                                                             gCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGat                                                                                     >  2:812288/1‑150 (MQ=255)

                                                             gCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGat                                                                                     <  1:732154/150‑1 (MQ=255)

                                                                   tAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCt                                                                               <  2:803826/150‑1 (MQ=255)

                                                                    cgcgcCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTg                                                                              <  2:1236579/149‑1 (MQ=255)

                                                                    aGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCATATGGACTTGTTTATCAATAATACCTGGGCAGATGATCTGCCCTCTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTg                                                                              <  1:1783076/150‑1 (MQ=255)

                                                                    aGCGCCACGTCCGGCGGGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTg                                                                              <  2:2585544/150‑1 (MQ=255)

                                                                       gcCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCa                                                                           >  2:1575643/1‑150 (MQ=255)

                                                                       gcCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCa                                                                           >  2:687006/1‑150 (MQ=255)

                                                                       gcCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCa                                                                           >  2:2230169/1‑150 (MQ=255)

                                                                       gcCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGAGCTGCCCACTGAGATCGACAACCGTGCAGTGCGGTACAATGTCAGAAGGGATATTGCTGGCa                                                                           >  1:1289398/1‑150 (MQ=255)

                                                                          aCTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAACACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACg                                                                        >  1:416881/1‑150 (MQ=255)

                                                                           cTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACgg                                                                       <  2:744761/150‑1 (MQ=255)

                                                                           cTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACgg                                                                       <  2:1383977/150‑1 (MQ=255)

                                                                            ttCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAACTGGACGTGTTGATCAATAACACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGc                                                                      <  2:1475686/150‑1 (MQ=255)

                                                                            gtCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGCTCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACggt                                                                      <  2:2384390/149‑2 (MQ=255)

                                                                             tCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCg                                                                     <  1:1938614/150‑1 (MQ=255)

                                                                             tCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCg                                                                     <  1:1591164/150‑1 (MQ=255)

                                                                             tCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCg                                                                     <  1:572284/150‑1 (MQ=255)

                                                                             tCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCg                                                                     <  1:2511762/150‑1 (MQ=255)

                                                                             tCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCg                                                                     <  2:2050377/150‑1 (MQ=255)

                                                                                 gCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATaa                                                                 <  1:23576/150‑1 (MQ=255)

                                                                                 gCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATaa                                                                 <  1:691613/150‑1 (MQ=255)

                                                                                     gcgcGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAAtttt                                                             <  1:148959/150‑1 (MQ=255)

                                                                                        cgtcgtGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCCCACCTGGGCAGAGGATCTGCCCACGGAGATCGACACCCGTGCAGTTCGGTACAATGTCAGAAGGGATAGTGCTGGCAACGGCGATAATTTTGcc                                                          >  1:1566439/1‑150 (MQ=255)

                                                                                          tcgtGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGt                                                        >  2:401496/1‑150 (MQ=255)

                                                                                               ggACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGcg                                                   <  1:1795755/150‑1 (MQ=255)

                                                                                                  ccTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCCCACCTGGGCAGAGGATCTGCCCACGGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGcgacg                                                >  1:587286/1‑150 (MQ=255)

                                                                                                  ccTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGcgacg                                                >  1:2586755/1‑150 (MQ=255)

                                                                                                    tGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTCCAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAg                                              >  2:1410656/1‑150 (MQ=255)

                                                                                                    tGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAg                                              >  2:1177185/1‑150 (MQ=255)

                                                                                                       ttCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATCCAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGAc                                           >  1:2533516/1‑150 (MQ=255)

                                                                                                       ttCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGAc                                           >  1:799293/1‑150 (MQ=255)

                                                                                                             gccaccgccaATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCa                                     <  2:443953/150‑1 (MQ=255)

                                                                                                             gccaccgccaATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCa                                     <  2:2311878/150‑1 (MQ=255)

                                                                                                               caccgccaATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCaaa                                   <  2:12494/150‑1 (MQ=255)

                                                                                                                accgccaATCAAATGGACGTGTTGATCAATCCCACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAAt                                  >  1:1170846/1‑150 (MQ=255)

                                                                                                                accgccaATCAAATGGACGTGTTGATCAATCCAACCTGGGCAGAGGATCTGCCCACGGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAAt                                  >  1:2093033/1‑150 (MQ=255)

                                                                                                                accgccaATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAAt                                  >  2:420472/1‑150 (MQ=255)

                                                                                                                    ccaATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTccc                              <  1:601096/150‑1 (MQ=255)

                                                                                                                    ccaATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTccc                              <  2:1632819/150‑1 (MQ=255)

                                                                                                                    ccaATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTccc                              <  2:525972/150‑1 (MQ=255)

                                                                                                                    ccaATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTccc                              <  1:1205116/150‑1 (MQ=255)

                                                                                                                    ccaAACAAAGGGACGTGTTGATCAATAAAACCTGGGCACAGGATCTGCCCACTGACATCGACAACCGAGCACTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTccc                              <  2:2460792/150‑1 (MQ=255)

                                                                                                                        tCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGAt                          >  2:211019/1‑150 (MQ=255)

                                                                                                                        tCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGAt                          >  1:2213592/1‑150 (MQ=255)

                                                                                                                        tCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGAt                          >  1:2632707/1‑150 (MQ=255)

                                                                                                                            aTGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCTGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTc                      <  1:995666/150‑1 (MQ=255)

                                                                                                                              ggACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCg                    >  1:2075746/1‑150 (MQ=255)

                                                                                                                              ggACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCg                    >  2:1439810/1‑150 (MQ=255)

                                                                                                                              ggACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCg                    >  1:641737/1‑150 (MQ=255)

                                                                                                                                   tgtTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCa               >  2:1741878/1‑150 (MQ=255)

                                                                                                                                   tgtTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCa               <  1:2278768/150‑1 (MQ=255)

                                                                                                                                   tgtTGATCAATAAAACCTGGGAAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCa               <  2:2181760/150‑1 (MQ=255)

                                                                                                                                     ttGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATa             >  1:140956/1‑150 (MQ=255)

                                                                                                                                        aTCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACaa          >  2:291668/1‑150 (MQ=255)

                                                                                                                                          cAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAAt        <  2:1900811/150‑1 (MQ=255)

                                                                                                                                           aaTAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATg       >  1:2400444/1‑150 (MQ=255)

                                                                                                                                               aaaCCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCt   >  1:1006444/1‑150 (MQ=255)

                                                                                                                                                aaCCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTc  >  2:511802/1‑150 (MQ=255)

GGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGGCGTGCGCGTCGTGGGACCTGCTTCGCCGCCACCGCCAATCAAATGGACGTGTTGATCAATAAAACCTGGGCAGAGGATCTGCCCACTGAGATCGACAACCGTGCAGTTCGGTACAATGTCAGAAGGGATATTGCTGGCAACGGCGATAATTTTGCCGTTAGCGACGAGGACATCGCAAATTCCCCGATCTTCCGGCGCATACAAATGCGCTC  >  NC_000913/4559193‑4559486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`