Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 107,554 G→T 16.6% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5540GT16.6% 395.8 / 26.2 145intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base G (20/97);  new base T (24/4);  total (44/101)
Fisher's exact test for biased strand distribution p-value = 1.12e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

ATGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATAA  >  NC_000913/107407‑107703
                                                                                                                                                     |                                                                                                                                                      
atGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGaa                                                                                                                                                      <  1:1002934/150‑1 (MQ=255)
 tgtgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAAt                                                                                                                                                     <  1:2094750/150‑1 (MQ=255)
   tgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTGTAttt                                                                                                                                                   >  2:324337/1‑150 (MQ=255)
   tgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTGTAttt                                                                                                                                                   >  2:324342/1‑150 (MQ=255)
     aCCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTta                                                                                                                                                 <  1:1510673/150‑1 (MQ=255)
       cTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTttta                                                                                                                                               >  2:2474739/1‑150 (MQ=255)
       cTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTtata                                                                                                                                               >  2:1498496/1‑150 (MQ=255)
          ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAaga                                                                                                                                            <  1:2163136/150‑1 (MQ=255)
           cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTTAATTTTATAagag                                                                                                                                           >  2:1828047/1‑150 (MQ=255)
           cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTTAATTTTTTAatag                                                                                                                                           >  2:852516/1‑150 (MQ=255)
           cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTGTAATTTATAagag                                                                                                                                           >  2:1500676/1‑150 (MQ=255)
              gacgacGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTTTATTTTATaaaatcca                                                                                                                                        >  1:2349381/1‑144 (MQ=255)
              gacgacGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGTATTTTTTaatatgtg                                                                                                                                        >  1:2124240/1‑144 (MQ=255)
                    gCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTTAATTTTATAATAGCT‑ATaaaaa                                                                                                                                 >  2:1394889/1‑150 (MQ=255)
                     cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACTTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCC‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATaaaatt                                                                                                                                <  2:1440021/150‑3 (MQ=255)
                     cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATaaaatt                                                                                                                                <  2:348881/150‑3 (MQ=255)
                      aGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAAc                                                                                                                               <  1:2220565/150‑1 (MQ=255)
                          cTGCCGTTGGCCTTCAACGCGCCTTCAGCTGTACTGGAATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCT‑GGTGAACCT‑GTCGTTTTTTTTTTAATTTTATAAGAGCT‑ATAAAAAACGGTg                                                                                                                           <  1:507861/150‑1 (MQ=255)
                          cTGCCGTTGGCCTTCAAAGCGCCTTCTGCTGTACTGGAATAACTACATTTATACTGTCTTATAAAATTCGACTGGCAAATCGGGCACTCTCTCCGGCCA‑GGTGAACCT‑GTCTTTTTTTTTTTAATTTTATAAGAGCT‑ATAAAAAACGGTg                                                                                                                           <  1:507781/150‑1 (MQ=255)
                          cTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACTTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCT‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTg                                                                                                                           <  1:46192/150‑1 (MQ=255)
                          cTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCT‑GGTGAACCA‑GTCTTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTg                                                                                                                           <  1:177350/150‑1 (MQ=255)
                           tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGTATTTTTTTATATGT‑ATAAAAAAAGGttg                                                                                                                          >  2:1875331/1‑148 (MQ=255)
                           tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGc                                                                                                                          <  2:2124667/150‑1 (MQ=255)
                           tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTTTATTTTTTAAAATAG‑ATaaaaaaaagggg                                                                                                                          >  2:1230953/1‑144 (MQ=255)
                           tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGGACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAAAGCT‑ATAAAAAAAGGTGc                                                                                                                          >  2:211596/1‑150 (MQ=255)
                           tGCCGTTGGCCTTAAAAGCGCATTCAGCTGTACTGGCATAACGACTTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCT‑GGTGAACCATTTTTTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTg                                                                                                                           <  1:2539837/150‑1 (MQ=255)
                              cGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCTTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCT‑GGTGAACCA‑TTCGTTTTTTTTTGATTTTTATAAGAGCT‑ATAAAAAACGGTGCGaa                                                                                                                       <  1:1155561/150‑1 (MQ=255)
                               gTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTTTATTTTATAAAAGCTCAAAAAAAACgggagccc                                                                                                                       >  1:2588663/1‑144 (MQ=255)
                                ttGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACg                                                                                                                     <  2:1186996/150‑1 (MQ=255)
                                   gCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTTTATTTTATAAAATCC‑ATAAAAAAAAGTGGGGACGcgg                                                                                                                  >  2:1294311/1‑148 (MQ=255)
                                   gCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTGAAATTTATAAAAGCT‑ATAAAAAAACGGGCCAACGcgg                                                                                                                  >  2:1784565/1‑148 (MQ=255)
                                    ccTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTTAATTTTTTTAGATCT‑ATAAAAAAAAGTGAGAAAGCAGt                                                                                                                 >  2:2704735/1‑150 (MQ=255)
                                     cTTCAACGCGCCTTCAGCTGTACTGGCATACCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGtt                                                                                                                <  1:1260966/150‑1 (MQ=255)
                                      ttCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTTTATTTTATAAGATCG‑ATAAAAAAATGTGGGAGAGCTGttt                                                                                                               >  1:1434832/1‑150 (MQ=255)
                                      tacAAAGCGCCTTCAGCAGTACATCCAAAACCACATAAATACTGTCCTATAAAATTCGACTGGCAAATCAGCCACTCTCTACGGCCA‑GGTGAACCA‑GTCGATATTTTATTAATATTATAAAAGAC‑AAAAAAAACCATACCAACGCttgat                                                                                                               >  2:957845/3‑146 (MQ=255)
                                       tCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGtttt                                                                                                              <  2:2639234/150‑1 (MQ=255)
                                          aaGCGCCTTCAGCTTTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCtt                                                                                                           <  2:1662573/150‑1 (MQ=255)
                                           aGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCG‑TTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                                                                                         <  1:1849567/150‑1 (MQ=255)
                                            gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                                                                                         <  1:504145/150‑1 (MQ=255)
                                            gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                                                                                         <  1:1557430/150‑1 (MQ=255)
                                            gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                                                                                         <  1:1537125/150‑1 (MQ=255)
                                             cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                                                                                        <  2:1699894/150‑1 (MQ=255)
                                             cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                                                                                        <  2:2139100/150‑1 (MQ=255)
                                               ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTTTATTTTATTAGTTCT‑TTAAAAAAAGGTGCGAAAGCAGTTTTGTTaataa                                                                                                      >  1:204739/1‑147 (MQ=255)
                                               ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTTTATTTTTTAATATCT‑AAAAAAAAACGTGGGAACCCAGTTTTGtttataa                                                                                                      >  1:204891/1‑145 (MQ=255)
                                                cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                                                                                     <  2:2736918/150‑1 (MQ=255)
                                                cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                                                                                     <  2:1703298/150‑1 (MQ=255)
                                                       tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCg                                                                                              <  2:904792/150‑1 (MQ=255)
                                                             ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                                                                                        <  1:403127/150‑1 (MQ=255)
                                                             ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                                                                                        <  1:403032/150‑1 (MQ=255)
                                                                 tAACGACATTTATACTGTCGTATAAAATTCGACTGGAAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt                                                                                    <  2:204870/150‑1 (MQ=255)
                                                                   aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCt                                                                                  <  2:1019241/150‑1 (MQ=255)
                                                                        aTTTATACGGTCGTATACAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt                                                                             <  2:197197/150‑1 (MQ=255)
                                                                            ttaCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGATCCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACTGTGCGAACGCTGTTTTCTTAAGCAATTTTCCGCACAACTTATCTTCATTCGTg                                                                         <  2:684614/149‑1 (MQ=255)
                                                                            ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTg                                                                         <  2:499059/150‑1 (MQ=255)
                                                                            ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTg                                                                         <  2:684678/150‑1 (MQ=255)
                                                                                tGTCTTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgt                                                                     <  2:1413567/150‑1 (MQ=255)
                                                                                tGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgt                                                                     <  2:1066651/150‑1 (MQ=255)
                                                                                tGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgt                                                                     <  1:132889/150‑1 (MQ=255)
                                                                                tGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgt                                                                     <  1:320498/150‑1 (MQ=255)
                                                                                  tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTTAAATTTATAAAAGCA‑AAAAAAAAACGTGCGAAACCGGTTTTGTTAATCAATTTTACGCACAACATATATTAATTAGTGCtgttg                                                                   >  2:1753305/1‑148 (MQ=255)
                                                                                  tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTGTTTTTTTGTAATTTATAAGAGCT‑ATAAAAAAACGTGCGCACCCGGTTTTGTTAATCACATTTACCCACAACATATCTTAATTAGTGGTgggg                                                                   >  2:194957/1‑150 (MQ=255)
                                                                                   cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGa                                                                  <  1:2214982/150‑1 (MQ=255)
                                                                                             tCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCtt                                                        <  2:2532060/150‑1 (MQ=255)
                                                                                               gACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTTTATTTTTTAATAGCA‑ATAAAAAAAGGTGCGAACGCTGTTTTGTTTAGCAATTTTACGCACAAATTATCTTCAATCGTGGTGTGGGCTGCAGGCTTTa                                                      >  2:930160/1‑150 (MQ=255)
                                                                                                           tGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGATCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGAttt                                          <  1:1549935/150‑1 (MQ=255)
                                                                                                           tGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGAttt                                          <  1:1107586/150‑1 (MQ=255)
                                                                                                           tGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGAttt                                          <  1:1498496/150‑1 (MQ=255)
                                                                                                           tGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGAttt                                          <  1:2704735/150‑1 (MQ=255)
                                                                                                           tGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGAttt                                          <  1:2474739/150‑1 (MQ=255)
                                                                                                             gccctctctCCGGCCA‑GGTGAACCC‑GGCGTTTTTTTTTGAATTGTATAAGAGCT‑ATCAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtgt                                        <  2:1407120/147‑1 (MQ=255)
                                                                                                             gCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtgt                                        <  2:878349/150‑1 (MQ=255)
                                                                                                             gCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtgt                                        <  2:391686/150‑1 (MQ=255)
                                                                                                               aCTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgc                                      <  1:773759/150‑1 (MQ=255)
                                                                                                               aCTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgc                                      <  2:1083219/150‑1 (MQ=255)
                                                                                                                 tctctcCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcgc                                    <  2:520214/150‑1 (MQ=255)
                                                                                                                 tctctcCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcgc                                    <  2:1027775/150‑1 (MQ=255)
                                                                                                                 tctctcCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcgc                                    <  1:2688068/150‑1 (MQ=255)
                                                                                                                 tctctcCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCG‑ATAAAAAACGGGGCGAACGCCGGTTTGTTAAGCACATTTACGCACAACATATCTTCATTAGTTCGGTGGACAGCAGGCTTTAAATATAATATTTGTgcgc                                    >  2:1574632/1‑150 (MQ=255)
                                                                                                                 tctctcCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATGTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcgc                                    <  2:2357393/150‑1 (MQ=255)
                                                                                                                   tctcCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTa                                  <  1:410468/150‑1 (MQ=255)
                                                                                                                    ccccGGCCATGTTGAACAG‑GTGGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTa                                  <  2:1645762/148‑1 (MQ=255)
                                                                                                                      ccGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAAt                               <  1:707618/150‑1 (MQ=255)
                                                                                                                            a‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGttt                         <  2:2632123/150‑1 (MQ=255)
                                                                                                                                tGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGaa                      <  1:885271/150‑1 (MQ=255)
                                                                                                                                tGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGaa                      <  2:930991/150‑1 (MQ=255)
                                                                                                                                tGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGaa                      <  1:2480200/150‑1 (MQ=255)
                                                                                                                                   aCCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAatat                   <  2:2453009/150‑1 (MQ=255)
                                                                                                                                     cA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGa                 <  1:1741255/150‑1 (MQ=255)
                                                                                                                                          cGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCgg             <  2:771271/150‑1 (MQ=255)
                                                                                                                                               ttttttGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGCACGCTGTTTTCTTAAGCACGTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATgg        <  1:542608/150‑1 (MQ=255)
                                                                                                                                               ttttttGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATgg        <  2:2319620/150‑1 (MQ=255)
                                                                                                                                               ttttttGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATgg        <  1:608328/150‑1 (MQ=255)
                                                                                                                                                tttttGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGc       <  1:2406688/150‑1 (MQ=255)
                                                                                                                                                tttttGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGc       <  2:2585921/150‑1 (MQ=255)
                                                                                                                                                    tGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATa   >  2:2146998/1‑150 (MQ=255)
                                                                                                                                                    tGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATa   >  2:1610835/1‑150 (MQ=255)
                                                                                                                                                    tGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATa   >  2:763676/1‑150 (MQ=255)
                                                                                                                                                     gAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATaa  >  2:40556/1‑150 (MQ=255)
                                                                                                                                                     gAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATaa  >  1:1873633/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCA‑GGTGAACCA‑GTCGTTTTTTTTTGAATTTTATAAGAGCT‑ATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATAA  >  NC_000913/107407‑107703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑