Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,412 C→A 7.3% G48C (GGC→TGC)  ybcI ← DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,4120CA 7.3% 507.6 / 2.1 160G48C (GGC→TGC) ybcIDUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (86/56);  new base A (2/16);  total (88/72)
Fisher's exact test for biased strand distribution p-value = 7.10e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

ACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCG  >  NC_000913/556265‑556561
                                                                                                                                                   |                                                                                                                                                     
aCGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCaa                                                                                                                                                     >  2:536177/1‑150 (MQ=255)
    gaCGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAAttt                                                                                                                                                 <  1:2527026/150‑1 (MQ=255)
    gaCGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAAttt                                                                                                                                                 >  1:1614326/1‑150 (MQ=255)
    gaCGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAAttt                                                                                                                                                 <  1:2363813/150‑1 (MQ=255)
        gTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGTCCAAAAACATTGCCGTAAGCAACGCCAAATTTAAAc                                                                                                                                             <  2:2400426/150‑1 (MQ=255)
         ttAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACg                                                                                                                                            >  1:1909986/1‑150 (MQ=255)
          tAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTACGCAACGCCAAATTTAAACGa                                                                                                                                           >  1:2278671/1‑150 (MQ=255)
          tAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGa                                                                                                                                           >  1:208644/1‑150 (MQ=255)
          tAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGa                                                                                                                                           >  1:1419369/1‑150 (MQ=255)
          tAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGAAGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGa                                                                                                                                           >  2:1356160/1‑150 (MQ=255)
           aaaaaCAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAATCGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAATCATTGCCGTAAGCAACGCCAAATTTAAACGAc                                                                                                                                          >  2:2158227/1‑150 (MQ=255)
           aaaaaCAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCACCGCCAAATGTAAACGAc                                                                                                                                          >  2:2000390/1‑150 (MQ=255)
           aaaaaCAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGAc                                                                                                                                          >  2:619459/1‑150 (MQ=255)
           aaaaaCAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGAc                                                                                                                                          >  2:2158252/1‑150 (MQ=255)
            aaaaCAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACa                                                                                                                                         >  1:1284154/1‑150 (MQ=255)
            aaaaCAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACa                                                                                                                                         >  1:304647/1‑150 (MQ=255)
            aaaaCAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACa                                                                                                                                         >  2:1331310/1‑150 (MQ=255)
                                  cccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCAt                                                                                                                   >  1:2443978/1‑150 (MQ=255)
                                   ccagcccTTAACCATCGGCGTCCAATAAACACAAATAACAGCGGTACAACAACCCAAAACACCAGCGATTGGGTAAACCCGCTATGACAAAAAACATGGCCGTAACCAACGCAAAATTTACACGACAATACGTCGGCGTCGGGCAGCATc                                                                                                                  <  1:1678922/150‑1 (MQ=255)
                                   ccagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAACCATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATc                                                                                                                  <  2:2217819/150‑1 (MQ=255)
                                   ccagcccTGAACCATCGGCGTCCAAGACACACACATCACCGCGGGACAACAACCGCAAACACCCGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATc                                                                                                                  <  2:2027149/150‑1 (MQ=255)
                                   ccagccaTTAACCATCGGCGTACAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAATCCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAATTTTAAACGACAATACGTCGGCGTCGGGCAGCATc                                                                                                                  <  2:2027138/150‑1 (MQ=255)
                                    cagcccTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCg                                                                                                                 >  2:430575/1‑150 (MQ=255)
                                             aCCCTCGGCGTCAAATAACCACACACACCCGCGGGACAAAAAACCCAAACACCAGCTATTGGTTAAACCCGCTATCCCCAAAAACATGGCCTTAAGCAACGCCAAATTTACACGACAATACGTCGGCGTCGGGCAGCATCGCCAGgatga                                                                                                        <  1:1169631/150‑1 (MQ=255)
                                                   ggCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTACACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCgg                                                                                                  <  1:1520895/150‑1 (MQ=255)
                                                   ggCGTCAAATAAACACACATACCCGCGGGAAAACAAACGAAAACACCCGCGAATGGGTAAACCCGCGATGACAAAAAACATTGCCTTAAGAAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCgg                                                                                                  <  1:633423/150‑1 (MQ=255)
                                                     cGTCCAATAAACCCCCATACCCGCGGGACAACAAACGCAAACACCAGCGACGGGGTAAACCCGCGATGACCAAAACCATTGCGGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCa                                                                                                <  1:1748229/150‑1 (MQ=255)
                                                     cGTCCAAAAACCACACAAACCCGCGGGACAACAAACCCAAACACCAGCGAATGGTAAAACCCGCGATGACCAAAAACATGGCCGTAACCAACGCAAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCa                                                                                                <  1:1177606/150‑1 (MQ=255)
                                                       tCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCaaa                                                                                              <  2:1909986/150‑1 (MQ=255)
                                                          aaTAACCCCACCTACCCGCGGGACAACAAACGCAAACACCAGCGAATGGTTAACCCCGCGATGACCAAAAACTTGGCCGTACGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATaa                                                                                           <  1:1669385/150‑1 (MQ=255)
                                                          aaTAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATac                                                                                           >  2:1410722/1‑149 (MQ=255)
                                                          aaTAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATaa                                                                                           >  2:1911819/1‑150 (MQ=255)
                                                           aTAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAAc                                                                                          <  2:1513267/150‑1 (MQ=255)
                                                           aTAAACACACAAAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCTATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAAc                                                                                          <  1:658805/150‑1 (MQ=255)
                                                              aaCACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGa                                                                                       >  2:896070/1‑150 (MQ=255)
                                                                 acacaTAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGAcgc                                                                                    >  1:968702/1‑150 (MQ=255)
                                                                  cacaTAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGAcgcg                                                                                   <  1:1546810/150‑1 (MQ=255)
                                                                   acaTAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCgg                                                                                  >  2:1285166/1‑150 (MQ=255)
                                                                   acaTAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCgg                                                                                  >  2:1813988/1‑150 (MQ=255)
                                                                   acaTAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCgg                                                                                  >  1:2656557/1‑150 (MQ=255)
                                                                   acaTAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCgg                                                                                  >  2:2502106/1‑150 (MQ=255)
                                                                   acaTAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCAGCGCCAGGATGATTCCGGCAAATAACAGACGCgg                                                                                  >  2:1285190/1‑150 (MQ=255)
                                                                     aTAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAg                                                                                >  2:1007206/1‑150 (MQ=255)
                                                                      taccAGCGGCACAACAAACGCAAACACCAGCGAATGGTTAAACCCGCGATGACCAAAAACATTGCCGTAACCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAgg                                                                               <  1:2117180/147‑1 (MQ=255)
                                                                      tAACAGCGGGACAACAAACGCAAACACCAGCGATTGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAgg                                                                               <  1:2395608/150‑1 (MQ=255)
                                                                      tAACAGCGGGACAACAAACGAAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAgg                                                                               <  2:1224204/150‑1 (MQ=255)
                                                                        aCAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAACATTGCCGTAAGCAACGCAAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGAt                                                                            <  1:111234/150‑1 (MQ=255)
                                                                         ctgCGGGAAAACAAACCCAAACACCAGCGATTGTTTAAACCCGCTTTGACAAAAAACATTGCCTTAAGAACCGCAAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGAt                                                                            <  1:472338/148‑1 (MQ=255)
                                                                         ccgCGGTACAAAAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGAAACGCAAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGAt                                                                            <  1:1634685/148‑1 (MQ=255)
                                                                         cAGCGGTACAACAACCGCAAACACCAGCGATTGGTTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCAAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGAt                                                                            <  1:373642/150‑1 (MQ=255)
                                                                         cAGCGGGAAAACAAACGAAAACACCAGCTATTGGTTAAACCCGCGATGACAAAAAACATTGCCGTAAGCAACGCAAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGAt                                                                            <  1:472281/150‑1 (MQ=255)
                                                                           gCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGa                                                                          >  1:1057660/1‑150 (MQ=255)
                                                                           gCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGa                                                                          >  1:22625/1‑150 (MQ=255)
                                                                                acaacaACCGCAAACACCCGCGAATGGGTAAACCCGCGAGGACCAAAAACATTGCCGTAAGAAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTc                                                                     <  1:1960066/150‑1 (MQ=255)
                                                                                acaacaAACGCAAACACCAGCGAATGGGTAACCCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTc                                                                     <  1:2017210/150‑1 (MQ=255)
                                                                                   acaaACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaa                                                                  <  2:304647/150‑1 (MQ=255)
                                                                                    caaACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaac                                                                 >  2:1086919/1‑150 (MQ=255)
                                                                                     aaaCGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaacc                                                                >  2:1192345/1‑150 (MQ=255)
                                                                                     aaaCGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaacc                                                                >  2:2718405/1‑150 (MQ=255)
                                                                                     aaaCGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaacc                                                                >  2:1642126/1‑150 (MQ=255)
                                                                                     aaaCGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCAAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaacc                                                                >  2:1192337/1‑150 (MQ=255)
                                                                                         gCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaacccaac                                                            >  1:1963160/1‑150 (MQ=255)
                                                                                         gCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaacccaac                                                            >  2:1136937/1‑150 (MQ=255)
                                                                                           aaaCACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGaacccaaccc                                                          >  1:1007737/1‑150 (MQ=255)
                                                                                               acaaGCGAATGGGTAAACCCGCCATGACAAAAAACATTGCCTTAAGCAACGCAAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTaaa                                                      <  2:2435286/147‑1 (MQ=255)
                                                                                               acCCGCGATTGGGTAAACCCGCGATGACCAAAAACAGTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTaaa                                                      <  2:2596913/150‑1 (MQ=255)
                                                                                               acCAGCTATTGGGTAAACCCGCGATGACCAAAACCTTTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTaaa                                                      <  1:296306/150‑1 (MQ=255)
                                                                                                ccAGCTAATGGGTAAACCCGCGATGACCAAAAACATTGCCTTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAAc                                                     <  1:1196188/150‑1 (MQ=255)
                                                                                                  aGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCa                                                   >  2:549306/1‑150 (MQ=255)
                                                                                                     gattGGGTAAACCCGCGATGACCAAAAACTTTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATg                                                <  1:1798411/147‑1 (MQ=255)
                                                                                                          gttAAACCCGCTATGACCAAAAACATTGCCGTAAGAAACGCAAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAg                                           <  1:571383/148‑1 (MQ=255)
                                                                                                           ttAAACACGATATTACCAAAAACTTTCCCTTAAGCAACGCAAAATTTAAACGAAAATACGTCGGCGTCGGGCAGCATCGCCAGGATTATTCCGTCAAATAACAGACGCGGATGTATGACTTTCGAACCCAACCCTAAACCAATGCATAgg                                          <  1:8399/149‑1 (MQ=255)
                                                                                                                 ccGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAAcg                                    >  2:1587993/1‑150 (MQ=255)
                                                                                                                 ccGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAAcg                                    >  1:1483347/1‑150 (MQ=255)
                                                                                                                  cgcgATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAAcgg                                   <  1:925495/150‑1 (MQ=255)
                                                                                                                    cgATGACCAAAAACTTTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAAcggcg                                 <  2:1757810/150‑1 (MQ=255)
                                                                                                                    cgATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAAcggcg                                 <  2:1757858/150‑1 (MQ=255)
                                                                                                                          ccAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtgc                           >  1:2500115/1‑150 (MQ=255)
                                                                                                                           cAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtgcg                          >  2:2519260/1‑150 (MQ=255)
                                                                                                                           cAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtgcg                          >  2:90606/1‑150 (MQ=255)
                                                                                                                           cAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtgcg                          >  2:1148483/1‑150 (MQ=255)
                                                                                                                            aaaaaaATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtgcgt                         >  2:83554/1‑150 (MQ=255)
                                                                                                                             aaaaCATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGta                        <  1:2279194/150‑1 (MQ=255)
                                                                                                                             aaaaCATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGta                        <  1:783783/150‑1 (MQ=255)
                                                                                                                              aaaCATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGtaa                       >  2:294608/1‑150 (MQ=255)
                                                                                                                                   ttGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACg                  <  1:1725074/150‑1 (MQ=255)
                                                                                                                                   ttGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACg                  <  1:2526009/150‑1 (MQ=255)
                                                                                                                                      ccGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGtaa                       >  1:98965/1‑142 (MQ=255)
                                                                                                                                       cGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTg              >  2:144346/1‑150 (MQ=255)
                                                                                                                                       cGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTg              >  1:1259446/1‑150 (MQ=255)
                                                                                                                                       cGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTg              >  2:2741187/1‑150 (MQ=255)
                                                                                                                                       cGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTg              <  2:2474576/150‑1 (MQ=255)
                                                                                                                                       cGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTg              >  2:144365/1‑150 (MQ=255)
                                                                                                                                        gTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTgg             >  1:2613844/1‑150 (MQ=255)
                                                                                                                                           aGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCat          >  1:2593027/1‑150 (MQ=255)
                                                                                                                                              aaCGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCAGAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCatgat       >  2:1197408/1‑150 (MQ=255)
                                                                                                                                               aCGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCatgatg      <  1:1007206/150‑1 (MQ=255)
                                                                                                                                               aCGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCatgatg      <  1:1007205/150‑1 (MQ=255)
                                                                                                                                                  ccAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTc   >  1:198725/1‑150 (MQ=255)
                                                                                                                                                   cAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCg  >  1:795865/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACGAGACGGTTAAAAACAGCCAGCAGCGAATCAGCCCAGCCCTGAACCATCGGCGTCCAATAAACACACATAACAGCGGGACAACAAACGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCG  >  NC_000913/556265‑556561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: