Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 940,754 G→A 8.3% intergenic (+34/‑205) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913940,7540GA 8.3% 475.3 / 22.3 144intergenic (+34/‑205)serS/dmsAseryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base G (63/69);  new base A (6/6);  total (69/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 160 total aligned reads displayed.

GGCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAAT  >  NC_000913/940606‑940903
                                                                                                                                                    |                                                                                                                                                     
ggCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCgg                                                                                                                                                      <  2:55199/150‑1 (MQ=255)
 gCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCggg                                                                                                                                                     >  2:345414/1‑150 (MQ=255)
       ggTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttt                                                                                                                                               >  1:253861/1‑150 (MQ=255)
         tCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttttt                                                                                                                                             >  2:2468876/1‑150 (MQ=255)
          cGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCttttta                                                                                                                                            <  2:1434931/150‑2 (MQ=255)
          cGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCCGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTCCTGAATCTAAAAAGCGCCCGCAGGCGCttttta                                                                                                                                            <  2:39914/150‑2 (MQ=255)
           gTACGCTGGTTGCCGTAATGGAAAACTATCCGCCGGCTGATGGTCGTATTGAAGTACCAGACGTTCGGCGTCCGTATATGAACGGACTGGAATATTTTGGCTAATACCCAATTTTTCTGAATCTAAACAGCGCCTGCGGGCGCttttttt                                                                                                                                           <  1:2642513/150‑1 (MQ=255)
           gTACGCTGGTTGCAGTAATTGAAAACTTTCAGAAGGCTGATGGTCGTATTGATGTTCCAGTTGTTCTGGGTCCGTATATGACCGGACTGTAATATATTGGCTACTACCCAATTTTTCTGAATCTTACAAGCGCCTGCGGGCGCttttttt                                                                                                                                           <  2:1588054/150‑1 (MQ=255)
           gTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGACTCTAAAAAGCGCCTGCGGGCGCttttttt                                                                                                                                           <  2:1588015/150‑1 (MQ=255)
            tACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTg                                                                                                                                          <  2:1799244/150‑1 (MQ=255)
             aCGCTGGGTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGt                                                                                                                                         <  2:2580775/150‑1 (MQ=255)
              cGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagatt                                                                                                                                        <  2:2553649/150‑6 (MQ=255)
              cGCTGGTTGCAGTAATGGAAAACTAGCCGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagatt                                                                                                                                        <  2:2335513/150‑6 (MQ=255)
                cTGGTTGCTGTAATGGAAAACTATCAGCTGGCTGATGGTCGTATTGAAGTACCAGCAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtctc                                                                                                                                      <  1:2073930/150‑1 (MQ=255)
                cTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtctc                                                                                                                                      <  1:1510875/150‑1 (MQ=255)
                cTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtctc                                                                                                                                      <  2:1160719/150‑1 (MQ=255)
                 tGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCATAAGTTCTGCGTCAGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAATCGCCTGCGGGCGCTTTTTTTGtatac                                                                                                                                     >  2:2459021/1‑146 (MQ=255)
                 tGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTcc                                                                                                                                     >  2:1719935/1‑150 (MQ=255)
                     tGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCttt                                                                                                                                 <  1:1367000/150‑1 (MQ=255)
                        aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTGTGCCTTTgtt                                                                                                                              >  2:699944/1‑148 (MQ=255)
                        aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGAt                                                                                                                              >  1:1165643/1‑150 (MQ=255)
                        aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGAt                                                                                                                              >  2:2106009/1‑150 (MQ=255)
                        aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGAt                                                                                                                              >  2:2107113/1‑150 (MQ=255)
                        aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTACCTTTGAt                                                                                                                              >  2:2353483/1‑150 (MQ=255)
                        aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTATACCTTTTAt                                                                                                                              >  2:699947/1‑150 (MQ=255)
                           aaTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAcc                                                                                                                           <  2:440864/150‑1 (MQ=255)
                            aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAACAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                          <  2:2750029/150‑1 (MQ=255)
                            aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                          <  2:544078/150‑1 (MQ=255)
                            aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                          <  2:1731356/150‑1 (MQ=255)
                            aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                          <  2:205210/150‑1 (MQ=255)
                                     tATCAGCAGGCTGTTGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataat                                                                                                                 <  1:1900224/150‑1 (MQ=255)
                                     tATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataat                                                                                                                 >  1:2295113/1‑150 (MQ=255)
                                      aTCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAAtt                                                                                                                <  1:124297/150‑1 (MQ=255)
                                        cagcagGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTAc                                                                                                              <  1:2246050/150‑1 (MQ=255)
                                                  aTGGTCGTATTTAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGATAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTa                                                                                                    <  1:1947311/150‑1 (MQ=255)
                                                  aTGGTCGTATTGAAGTACCAGATGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTa                                                                                                    <  1:1626298/150‑1 (MQ=255)
                                                  aTGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTa                                                                                                    <  1:1166210/150‑1 (MQ=255)
                                                           ttGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTACCATTGATAACGAACAATAAATACTCATAACATACACGAAAta                                                                                           >  1:1014496/1‑150 (MQ=255)
                                                                 tACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTCCCATTGATACCGAACAATAATTAATCCACACATACACGTAAtactaaat                                                                                     >  1:2459561/1‑147 (MQ=255)
                                                                     aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGa                                                                                 >  2:849810/1‑150 (MQ=255)
                                                                     aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGa                                                                                 <  1:2142205/150‑1 (MQ=255)
                                                                     aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCATTGATACCGAACAAAAATTACTCCTCACATACACCTAATACTACTTTCGa                                                                                 >  2:1037460/1‑150 (MQ=255)
                                                                          ttCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGTTACCGAACCAAAATTACTCCTCACTTACACGTGATAATACTTTAGAGTGaa                                                                            >  1:1879117/1‑150 (MQ=255)
                                                                          ttCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGaa                                                                            >  2:2498776/1‑150 (MQ=255)
                                                                          ttCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCGTTGATCCCGAACAATAATTACTCCTCACTTACACGTAATACGACTTTCGAGTGaa                                                                            >  2:2672216/1‑150 (MQ=255)
                                                                          ttCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTACCTTTGATAACGAACAAAAATTAATCCTCACTTACACGTAATACTACATTCGAGTGaa                                                                            >  1:2605267/1‑150 (MQ=255)
                                                                             tGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAAt                                                                         <  2:2583224/150‑1 (MQ=255)
                                                                             tGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAAt                                                                         <  2:253861/150‑1 (MQ=255)
                                                                              gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTCCCTTTGATAACGAACAAAAATTACTCCTCACTTACACGTAAAACAACATTCGAGTGaaaaac                                                                        >  1:178912/1‑148 (MQ=255)
                                                                              gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTCCCGTTGATAACGAAAAATAATTACAACACACATACACGAAATACAAAGATCGAGTTTAAATc                                                                        >  1:875609/1‑150 (MQ=255)
                                                                              gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTAATACACACTTACACGTAATAATAAATTCGAGTGAAAATc                                                                        >  1:1360071/1‑150 (MQ=255)
                                                                              gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCGTTGATACCGCACAATAATTACTCCTCACTTACACGGAATACTACGTTCGAGTGAAAATc                                                                        >  1:1545007/1‑150 (MQ=255)
                                                                              gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCATTGATAACGAACAAAAATTACACCACACGTACAACTAAAACTACATTCGAGTGAAAAta                                                                        >  1:641258/1‑149 (MQ=255)
                                                                                 tCCGTATATGAACGGACTGGAATATATTGGCTAAGACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTAc                                                                     <  1:716279/150‑1 (MQ=255)
                                                                                        atGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATctct                                                             >  1:1597174/1‑150 (MQ=255)
                                                                                              ggACTTGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                        <  2:2408892/150‑1 (MQ=255)
                                                                                              ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                        <  2:1089078/150‑1 (MQ=255)
                                                                                              ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                        <  2:1339559/150‑1 (MQ=255)
                                                                                              ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                        <  2:1452597/150‑1 (MQ=255)
                                                                                              ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                        <  1:2545914/150‑1 (MQ=255)
                                                                                               gACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTGTCCCGTTGTTACCGAACAAAAAATACTCCACACATACACGGAATACTACATTCGAGTTTAAATCTAACTATCTCTTTGAtt                                                       >  1:574238/1‑150 (MQ=255)
                                                                                               gACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCGTTTTTTGTATACCATTTATACCCAAAAAAAAATACTCCACAAAAACACGGAAAACAAAATTAGAGTGAAAAACTACCGATAACTTTGAtt                                                       >  1:1336046/1‑150 (MQ=255)
                                                                                                aCTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTGTCCCTTTGATAACCAAAAAAAAATAAAACTCACATAAACGGAAAAAAAATTTCGAGTTAAAAAATAACAATATCTTTGAttt                                                      >  1:1071102/1‑150 (MQ=255)
                                                                                                 cTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAtttt                                                     <  1:2106009/150‑1 (MQ=255)
                                                                                                 cTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAtttt                                                     <  2:1165643/150‑1 (MQ=255)
                                                                                                 cTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAtttt                                                     <  1:699944/150‑1 (MQ=255)
                                                                                                  tGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTATCCCTTTGATACCGCAAAAAAATTAATCCTCACATACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTc                                                    >  2:2182367/1‑150 (MQ=255)
                                                                                                      atatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAat                                                <  2:1749405/150‑1 (MQ=255)
                                                                                                      atatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAat                                                <  2:780729/150‑1 (MQ=255)
                                                                                                      atatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAat                                                <  2:2022085/150‑1 (MQ=255)
                                                                                                       tatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTGTACCATTGTTACCGAACAAAAAATAATAATCAAATAAACCTAAAACAAAATTAGAGTGTAAATATAACAATATATTTTATTTTaaaaat                                               >  1:1120991/1‑148 (MQ=255)
                                                                                                       tatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCGTTTTTTGTCTCCCATTGATACCCAACAAAAAATAATACACACAGACACCTAAAAATACATTAGAGTGAAAATAAACCAATATAGTTTATTTTaaaaat                                               >  1:1473208/1‑148 (MQ=255)
                                                                                                             ggCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGa                                         <  1:37441/150‑1 (MQ=255)
                                                                                                             ggCTAATACCAAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGa                                         <  2:2490472/150‑1 (MQ=255)
                                                                                                                    aCCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACa                                 <  1:340866/150‑1 (MQ=255)
                                                                                                                     cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACa                                 >  2:1806354/1‑150 (MQ=255)
                                                                                                                     cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCGTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATAcc                                 >  2:1143003/1‑149 (MQ=255)
                                                                                                                     cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGGCTCCCTTTGATCCCCAACAATAATTACTCCACACTTACACGGAATACGACTTTCGAGTGAAAATCTACCTATCTCATTGATTTTCAAATTATTCGATGTATACa                                 >  2:419668/1‑150 (MQ=255)
                                                                                                                        aaTTTTTCTGAATCTCAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTGGATACCGAACAATAATTACTCCTCACTTACACGTAATACTCCTTTCGAGTGAAAATCTACCTCTCACTTTGATTTTCAAATTATTCGATGTATACAAGc                              <  2:2292442/150‑1 (MQ=255)
                                                                                                                        aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc                              <  2:2244228/150‑1 (MQ=255)
                                                                                                                        aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc                              <  1:32948/150‑1 (MQ=255)
                                                                                                                        aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc                              <  2:1025653/150‑1 (MQ=255)
                                                                                                                           ttttCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATTCCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta                           >  2:2521260/1‑150 (MQ=255)
                                                                                                                              tCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtatat                        <  1:2119044/150‑1 (MQ=255)
                                                                                                                               cTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTGTCCCTTTGATACCGAACAAGAATTAATCAACACTTAAACGGAATAATAAATTCGTGTGAAAATATACCTATATCTTTTATTTTCAAATTATTCGCTGTATACAAGCCgatatt                       >  2:471984/1‑149 (MQ=255)
                                                                                                                               cTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtatata                       >  2:1138058/1‑150 (MQ=255)
                                                                                                                                 gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGc                     <  1:1292264/150‑1 (MQ=255)
                                                                                                                                 gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGc                     <  1:1107957/150‑1 (MQ=255)
                                                                                                                                     aaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAAc                 >  2:792128/3‑150 (MQ=255)
                                                                                                                                     aaaaaaaGCGCCCGCAGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACt                >  2:447246/3‑150 (MQ=255)
                                                                                                                                     aaaaaaaGCGCCCGCAGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACt                >  2:447251/3‑150 (MQ=255)
                                                                                                                                          aaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGa                                                                                 >  2:1964077/1‑81 (MQ=255)
                                                                                                                                          aaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGa                                                                                 <  1:1964077/81‑1 (MQ=255)
                                                                                                                                              gcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGa        <  1:1022471/150‑1 (MQ=255)
                                                                                                                                               ccTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaa       <  1:2468876/150‑1 (MQ=255)
                                                                                                                                                    gggCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAattaat  <  1:2532811/150‑1 (MQ=255)
                                                                                                                                                    gggCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataat  <  1:612478/150‑1 (MQ=255)
                                                                                                                                                    gggCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataat  <  1:558235/150‑1 (MQ=255)
                                                                                                                                                    gggCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataat  <  1:2540781/150‑1 (MQ=255)
                                                                                                                                                    gggCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataat  <  1:2283005/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAAT  >  NC_000913/940606‑940903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: