Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,592,187 G→T 5.6% L86M (CTG→ATG)  hipB ← antitoxin of HipAB toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,592,1870GT 5.6% 606.8 / 7.2 170L86M (CTG→ATG) hipBantitoxin of HipAB toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (84/71);  new base T (5/9);  total (90/80)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

AGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAATCGTCGCCTGCTTAATAC  >  NC_000913/1592043‑1592336
                                                                                                                                                |                                                                                                                                                     
aGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGAtttt                                                                                                                                                  >  1:1902985/1‑150 (MQ=255)
aGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGAtttt                                                                                                                                                  >  1:794899/1‑150 (MQ=255)
aGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGAtttt                                                                                                                                                  >  1:549445/1‑150 (MQ=255)
aGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGAtttt                                                                                                                                                  >  1:553303/1‑150 (MQ=255)
aGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGAtttt                                                                                                                                                  >  1:553329/1‑150 (MQ=255)
 gCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTg                                                                                                                                                 <  1:1602295/150‑1 (MQ=255)
 gCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTg                                                                                                                                                 <  2:1660573/150‑1 (MQ=255)
   gAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGct                                                                                                                                               >  1:2688975/1‑150 (MQ=255)
   gAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGct                                                                                                                                               >  1:363088/1‑150 (MQ=255)
    aaaGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctg                                                                                                                                              >  2:812175/1‑150 (MQ=255)
    aaaGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctg                                                                                                                                              >  2:2257996/1‑150 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          <  1:870154/150‑1 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          >  1:2214141/1‑150 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          <  1:2673414/150‑1 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          >  1:101080/1‑150 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          <  1:913097/150‑1 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          >  2:931663/1‑150 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          >  2:918058/1‑150 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          <  1:1508688/150‑1 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          >  2:534718/1‑150 (MQ=255)
        tGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttct                                                                                                                                          <  1:702341/150‑1 (MQ=255)
           cAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGctgttctgtt                                                                                                                                       <  2:111675/150‑1 (MQ=255)
                   tGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTgg                                                                                                                               <  2:2659801/150‑1 (MQ=255)
                   tGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTgg                                                                                                                               >  1:2607563/1‑150 (MQ=255)
                   tGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTgg                                                                                                                               >  1:703552/1‑150 (MQ=255)
                    ggCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGc                                                                                                                              <  1:237792/150‑1 (MQ=255)
                     gCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCg                                                                                                                             >  1:1017985/1‑150 (MQ=255)
                     gCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCg                                                                                                                             >  2:546804/1‑150 (MQ=255)
                                  cTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTcgc                                                                                                                <  2:529747/150‑1 (MQ=255)
                                     aCCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCATATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTc                                                                                                             >  1:2279145/1‑150 (MQ=255)
                                     aCCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCATATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTc                                                                                                             >  1:2239397/1‑150 (MQ=255)
                                     aCCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCATATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTc                                                                                                             >  1:2097208/1‑150 (MQ=255)
                                     aCCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCATATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTc                                                                                                             >  1:2097173/1‑150 (MQ=255)
                                     aCCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTc                                                                                                             <  1:2273234/150‑1 (MQ=255)
                                     aCCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTc                                                                                                             <  1:141249/150‑1 (MQ=255)
                                     aCCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTc                                                                                                             <  1:141382/150‑1 (MQ=255)
                                     aCCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTc                                                                                                             <  1:585577/150‑1 (MQ=255)
                                      ccACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCg                                                                                                            >  1:185455/1‑150 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  1:780773/150‑1 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  2:946760/150‑1 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  1:1633865/150‑1 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  1:417735/150‑1 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  2:2648667/150‑1 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  1:294862/150‑1 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  2:2485534/150‑1 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  2:1846528/150‑1 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  2:129912/150‑1 (MQ=255)
                                           ccGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAg                                                                                                       <  2:1327150/150‑1 (MQ=255)
                                            cGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGc                                                                                                      <  2:946869/150‑1 (MQ=255)
                                            cGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGc                                                                                                      >  1:1707200/1‑150 (MQ=255)
                                            cGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGc                                                                                                      >  1:2175908/1‑150 (MQ=255)
                                            cGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGACATTTTTCGCGTCGCATAGc                                                                                                      <  2:2026472/150‑1 (MQ=255)
                                               tGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTc                                                                                                   >  1:1549665/1‑150 (MQ=255)
                                               tGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTc                                                                                                   >  1:1755995/1‑150 (MQ=255)
                                               tGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTc                                                                                                   <  1:2018291/150‑1 (MQ=255)
                                               tGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTc                                                                                                   >  1:1395413/1‑150 (MQ=255)
                                                 cATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCAt                                                                                                 >  2:2453842/1‑150 (MQ=255)
                                                    aCTTAAAGGGGGGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTga                                                                                              <  1:146653/150‑1 (MQ=255)
                                                     cTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTgag                                                                                             >  1:1117807/1‑150 (MQ=255)
                                                      ttAACGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCGACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTgaga                                                                                            <  2:1590876/150‑1 (MQ=255)
                                                      ttAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTgaga                                                                                            >  2:2312896/1‑150 (MQ=255)
                                                      ttAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCAGTgaga                                                                                            >  2:640606/1‑150 (MQ=255)
                                                                      cGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTaa                                                                            >  1:2445849/1‑150 (MQ=255)
                                                                      cGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTaa                                                                            >  1:2445732/1‑150 (MQ=255)
                                                                           gCTAACTTCGTTAACTCGCCTACCCGGTGTTTGTTCATCCATGTGACATGTTTAGGCATTACCACCCCAGTTTTTGCTGTTGTGTTGTTTCTGGCGAGGCTTTTTTCGGGTCGAATAGGGTAATTGAGAGTTCAAGCGACTGTAAAATCt                                                                       <  2:245700/150‑1 (MQ=255)
                                                                           gCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCATATTTTGCGGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCt                                                                       <  2:2410936/150‑1 (MQ=255)
                                                                            cTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCtt                                                                      >  2:2121919/1‑150 (MQ=255)
                                                                                 ttCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCGAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGAGTGTAATATCTTatttt                                                                 >  2:1805198/1‑146 (MQ=255)
                                                                                   cGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCCCTCCAGATTTTGCTGTTCTGTTGATTCGGGCGCGGCATTTTGCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAAt                                                               <  2:1022237/150‑1 (MQ=255)
                                                                                   cGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCCGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAAt                                                               <  2:1870978/150‑1 (MQ=255)
                                                                                     ttAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGt                                                             >  2:360489/1‑150 (MQ=255)
                                                                                       aaCTCGCCTACCCGCTGGTTGTTCATCCATGTGACATGTTTAGGCTTTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCg                                                           <  2:2446896/150‑1 (MQ=255)
                                                                                       aaCTCGCCTACCCGCTGGTTGTTCATCCACGTGACACGTTTCGGCATTACCCCTCCAGTTTTTGCTGTTGTGTTGTTTGTGGCGCGGCTTTTTTGGCGTCGCATCGCGTCATTGAGAGTTCACGCGACTGTAAAATCTTAAAAAATGTCg                                                           <  2:731785/150‑1 (MQ=255)
                                                                                       aaCTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTTTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCg                                                           <  2:1384031/150‑1 (MQ=255)
                                                                                       aaCTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGTGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCg                                                           <  2:1722889/150‑1 (MQ=255)
                                                                                        aCTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGt                                                          >  2:2076839/1‑150 (MQ=255)
                                                                                                  ccGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTa                                                >  2:2469014/1‑150 (MQ=255)
                                                                                                           gttCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAggg                                       >  2:581685/1‑150 (MQ=255)
                                                                                                            ttCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGgt                                      >  2:2255389/1‑150 (MQ=255)
                                                                                                              cATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGgttg                                    >  1:702846/1‑150 (MQ=255)
                                                                                                              cATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTAAGGgttg                                    >  1:2359660/1‑150 (MQ=255)
                                                                                                                 ccAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGttt                                 >  2:1796762/1‑150 (MQ=255)
                                                                                                                  cAAGTGACATGTTTAGGCATTACCACTCCATATTTTGCTGTTCTTTTTATTCTGGCGTGGCATTTTTCGCGTCGCATAGCTTAATTGAGATTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGtttt                                <  2:1225881/150‑1 (MQ=255)
                                                                                                                  cAAGTGACAAGTTTCGGAATTACCACTCCATATTTTGCTGTTCTTTTGATTCTGGCGAGGCATTTTTCGCGTCGCATCGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGtttt                                <  1:1508348/150‑1 (MQ=255)
                                                                                                                       taCAAGTTTAGGAATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGATTTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGaaa                           <  1:600652/149‑1 (MQ=255)
                                                                                                                          atgTTTAGTCATAACCACTCCTTATTTTGCTGTTGTGTTGATTCTGGCGTGGCTTTTTTCGCGTCGCATAGCTTCATTGAGTGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTg                        <  1:2117601/148‑1 (MQ=255)
                                                                                                                             tttAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGaa                     >  2:767891/1‑150 (MQ=255)
                                                                                                                             tttAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGaa                     >  1:339706/1‑150 (MQ=255)
                                                                                                                              ttcggCATTACCACTCCATATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGGGTCGCATAGCGTCATTGCGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGaaa                    <  2:1960054/147‑1 (MQ=255)
                                                                                                                               tctgCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGTGGCATTTTTCGCGTCGCATAGCGTCTTTGAGAGTTCACGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAAt                   <  2:1760625/147‑1 (MQ=255)
                                                                                                                               tAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAAt                   >  2:1252872/1‑150 (MQ=255)
                                                                                                                               tAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAAt                   >  2:1217562/1‑150 (MQ=255)
                                                                                                                                 ggCATTACCACTCCATATTTTGCTGTTCTGTTTTTTGTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTTTGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAAtcg                 <  2:2185833/150‑1 (MQ=255)
                                                                                                                                  gCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAAtcgt                >  2:467604/1‑150 (MQ=255)
                                                                                                                                    aTTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAAtcgtcg              >  1:1223191/1‑150 (MQ=255)
                                                                                                                                    aTTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGCCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAAtcgtcg              >  1:1223192/1‑150 (MQ=255)
                                                                                                                                     ttACCACTCCTGTTTTTGCTGTTCTGTTTATTCTGGCGAGGCATTTTTCGCGTCGAATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAATCGTCGc             <  1:812175/150‑1 (MQ=255)
                                                                                                                                     ttACCACTCCAGATTTTGCTGTTCTGTTGTTTCTGGCGTGGAATTTTGCGCGTCGCATAGCGACATTGATATTACAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAATCGTCGc             <  1:2257996/150‑1 (MQ=255)
                                                                                                                                         cACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAATCGTCGCCTGc         >  2:2138440/1‑150 (MQ=255)
                                                                                                                                         cACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAATCGTCGCCTGc         >  1:13712/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
AGCGAAAGTGACAACGGTCTGGCATAACGGCTTGCTAACCACTCCGGTGCATACTTAAAGGTGTGCGCGCCGTTGGCTAACTTCGTTAACTCGCCTACCCGCTGGTTGTTCATCCAAGTGACAAGTTTAGGCATTACCACTCCAGATTTTGCTGTTCTGTTGATTCTGGCGAGGCATTTTTCGCGTCGCATAGCGTCATTGAGAGTTCAAGCGACTGTAAAATCTTAAAAAATGTCGTGAGCGTGGTATTGTCAGGGTTGTTTTCGAAATTGGAAATCGTCGCCTGCTTAATAC  >  NC_000913/1592043‑1592336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: