Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,153,498 C→A 5.5% intergenic (+47/‑146) sibA → / → sibB sRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,4980CA 5.5% 452.1 / 8.9 124intergenic (+47/‑146)sibA/sibBsRNA antisense regulator of toxic IbsA protein/sRNA antisense regulator of toxic IbsB protein
Reads supporting (aligned to +/- strand):  ref base C (39/77);  new base A (5/3);  total (44/80)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

TTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTG  >  NC_000913/2153349‑2153646
                                                                                                                                                     |                                                                                                                                                    
ttAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGccc                                                                                                                                                      >  2:851438/1‑150 (MQ=255)
ttAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGccc                                                                                                                                                      >  2:250226/1‑150 (MQ=255)
        ccTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCa                                                                                                                                              <  1:1002651/150‑1 (MQ=255)
        ccTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCa                                                                                                                                              <  2:1747360/150‑1 (MQ=255)
        ccTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCa                                                                                                                                              <  2:1864035/150‑1 (MQ=255)
        ccTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCa                                                                                                                                              <  2:2733238/150‑1 (MQ=255)
        ccTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCa                                                                                                                                              <  2:658874/150‑1 (MQ=255)
            gAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCt                                                                                                                                          >  1:79310/1‑150 (MQ=255)
                 tAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGaa                                                                                                                                                  <  2:1527460/137‑1 (MQ=255)
                 tAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGaa                                                                                                                                                  >  1:1527460/1‑137 (MQ=255)
                   aTCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTa                                                                                                                                   <  2:1932293/150‑1 (MQ=255)
                    tCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTaa                                                                                                                                  <  1:224873/150‑1 (MQ=255)
                    tCACAAAGAGTATCACCAGTATGATGACGAGCTACAACATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTaa                                                                                                                                  <  2:403608/150‑1 (MQ=255)
                    acACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTAATAAAAGCCCTCTTCTCCGGGAGAGGCATTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTaa                                                                                                                                  <  2:403607/149‑1 (MQ=255)
                           gagTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGa                                                                                                                           <  1:1828768/150‑1 (MQ=255)
                           gagTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGa                                                                                                                           <  1:1828769/150‑1 (MQ=255)
                           gagTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGa                                                                                                                           <  1:294018/150‑1 (MQ=255)
                             gTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAAt                                                                                                                         <  2:357131/150‑1 (MQ=255)
                             gTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAAt                                                                                                                         <  2:553400/150‑1 (MQ=255)
                                 cACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                                                                                                                     <  1:1244670/150‑1 (MQ=255)
                                 cACCAGTATGATGACCTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATaaaa                                                                                                                     <  1:858776/150‑1 (MQ=255)
                                    cAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAg                                                                                                                  >  2:2256316/1‑150 (MQ=255)
                                       tATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGttt                                                                                                               <  1:1346431/150‑1 (MQ=255)
                                       tATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGttt                                                                                                               <  1:1958249/150‑1 (MQ=255)
                                        atgatgACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCTTCACGCTGAAATCATCGGATTACCTCCTTTCTCCCTGAATGCAGTATATCGCTAACGCGTGATTATAATAGTTTc                                                                                                              <  2:2400273/150‑1 (MQ=37)
                                        atgatgACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTc                                                                                                              <  2:888952/150‑1 (MQ=255)
                                          gatgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCct                                                                                                            >  2:2538238/1‑150 (MQ=255)
                                          gatgaCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCct                                                                                                            >  2:923744/1‑150 (MQ=255)
                                              aCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCTTCGGCTTACCTCCTTTCGCCCTGAAAGCAGTCTATCGCAAAACCGAAGATATAATAGTTGccacagt                                                                                                        >  2:1098376/1‑145 (MQ=17)
                                              aCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAAGCCGAGAATAAAATAGTTTCCTCtgt                                                                                                        >  2:2232593/1‑150 (MQ=255)
                                              aCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCg                                                                                                                                      <  1:1299968/120‑1 (MQ=255)
                                              aCGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCg                                                                                                                                      >  2:1299968/1‑120 (MQ=255)
                                                gTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGa                                                                                                      >  2:181839/1‑150 (MQ=255)
                                                   cTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGatta                                                                                                   >  2:2157412/1‑150 (MQ=255)
                                                   cTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGatta                                                                                                   >  2:2663891/1‑150 (MQ=255)
                                                   cTTCATCATAACCCGTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTTACGCGTGAATAATATAGTTTCCTCTGTGatta                                                                                                   >  1:1288571/1‑150 (MQ=255)
                                                     tcatcaTAACCCTTTCCTTATTACAAGCCCTCTACTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGattatt                                                                                                 <  1:2642701/150‑1 (MQ=255)
                                                     tcatcaTAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGattatt                                                                                                 <  1:1418737/150‑1 (MQ=255)
                                                      catcatAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTg                                                                                                <  2:2593029/150‑1 (MQ=255)
                                                           tAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTaa                                                                                           <  1:472138/150‑1 (MQ=255)
                                                           tAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTaa                                                                                           <  1:1709340/150‑1 (MQ=255)
                                                           tAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTaa                                                                                           <  1:1709345/150‑1 (MQ=255)
                                                           tAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTaa                                                                                           <  2:1212709/150‑1 (MQ=255)
                                                                cTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACt                                                                                      <  2:192726/150‑1 (MQ=255)
                                                                cTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACt                                                                                      <  2:1646734/150‑1 (MQ=255)
                                                                cTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACt                                                                                      <  2:196913/150‑1 (MQ=255)
                                                                cTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACt                                                                                      <  2:196922/150‑1 (MQ=255)
                                                                cTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACt                                                                                      <  2:869913/150‑1 (MQ=255)
                                                                      ttattaAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAAACATCGGCTTACCTCCTTTCGCCATGAAAGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAAGGGCTCGCgaa                                                                                >  2:857834/1‑150 (MQ=18)
                                                                         ttaAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCCAAGaa                                                                             >  1:1736669/1‑150 (MQ=255)
                                                                          taAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGCATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGAGTATTGCGTATCGCCTCGCGCAGaag                                                                            >  1:1927210/1‑149 (MQ=21)
                                                                                cccTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGc                                                                      >  2:1493982/1‑150 (MQ=255)
                                                                                 ccTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCt                                                                     >  1:2621291/1‑150 (MQ=255)
                                                                                 ccTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCt                                                                     >  1:1802309/1‑150 (MQ=255)
                                                                                 ccTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCt                                                                     >  1:2204216/1‑150 (MQ=255)
                                                                                 ccTCTACTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCt                                                                     >  1:2621285/1‑150 (MQ=255)
                                                                                   tcttctCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTgg                                                                   <  2:1298966/150‑1 (MQ=255)
                                                                                   tcttctCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAAAGCAGTCTATCGCTAACGCGTCGCTAGAGACTATTCCACGGTGATTATTGCGTAACGCCTCGCGAAGACGATTTGCTgg                                                                   >  2:361865/1‑150 (MQ=17)
                                                                                   tcttctCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTaa                                                                                                                                  <  2:2234178/87‑1 (MQ=11)
                                                                                   tcttctCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCATGCAAGCAGTCTATCGCTaa                                                                                                                                  >  1:2234178/1‑87 (MQ=11)
                                                                                      tctcCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAg                                                                >  1:40551/1‑150 (MQ=255)
                                                                                          cGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATc                                                            <  1:52808/150‑1 (MQ=255)
                                                                                           gggAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCg                                                           <  2:2013511/150‑1 (MQ=255)
                                                                                               gagGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATCGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGa                                                       <  1:1809952/150‑1 (MQ=255)
                                                                                                    tttCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCTGGATCGCAGACTACa                                                  >  2:215868/1‑150 (MQ=255)
                                                                                                    tttCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACa                                                  >  2:2124929/1‑150 (MQ=255)
                                                                                                     ttCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACaa                                                 <  1:1387498/150‑1 (MQ=255)
                                                                                                     ttCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACaa                                                 <  2:2084613/150‑1 (MQ=255)
                                                                                                       cccGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTc                                                                                                              >  2:2725870/1‑87 (MQ=255)
                                                                                                       cccGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTc                                                                                                              <  1:2725870/87‑1 (MQ=255)
                                                                                                            ttCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGc                                          <  2:798432/150‑1 (MQ=255)
                                                                                                             tCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTATCGCGTGAATAAAATAGTGTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCg                                         <  2:1646486/150‑1 (MQ=255)
                                                                                                             tCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCg                                         <  2:195409/150‑1 (MQ=255)
                                                                                                             tCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCg                                         <  2:545588/150‑1 (MQ=255)
                                                                                                               aGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGa                                       <  2:1143349/150‑1 (MQ=255)
                                                                                                               aGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATGTGCTGGCAGGATCGCAGACTCCAAAGCCTGCGGa                                       >  2:2058187/1‑150 (MQ=255)
                                                                                                                  gTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGAttc                                    <  1:1288885/150‑2 (MQ=255)
                                                                                                                  gTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTg                                    <  1:2176395/150‑1 (MQ=255)
                                                                                                                  gTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTg                                    <  1:1312713/150‑1 (MQ=255)
                                                                                                                  gTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTg                                    <  1:2548556/150‑1 (MQ=255)
                                                                                                                  gTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTg                                    <  1:1184738/150‑1 (MQ=255)
                                                                                                                   tCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGa                                   <  1:1605250/150‑1 (MQ=255)
                                                                                                                           aaaTCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTa                           <  2:1262200/150‑1 (MQ=255)
                                                                                                                           aaaTCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTa                           <  2:2069701/150‑1 (MQ=255)
                                                                                                                           aaaTCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTa                           <  1:2676715/150‑1 (MQ=255)
                                                                                                                           aaaTCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTa                           <  2:1986413/150‑1 (MQ=255)
                                                                                                                           aaaTCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTa                           <  2:317265/150‑1 (MQ=255)
                                                                                                                             atcatcGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATc                         <  1:2592403/150‑1 (MQ=255)
                                                                                                                                        aCCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATa              <  2:2398170/150‑1 (MQ=255)
                                                                                                                                         cctcctTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATAc             >  1:237968/1‑150 (MQ=255)
                                                                                                                                         cctcctTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATAc             >  1:2550965/1‑150 (MQ=255)
                                                                                                                                          ctcctTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACt            >  2:412351/1‑150 (MQ=255)
                                                                                                                                          ctcctTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACt            >  2:1171251/1‑150 (MQ=255)
                                                                                                                                           tcctTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACtt           >  2:866806/1‑150 (MQ=255)
                                                                                                                                           tcctTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATGGACAATCTTATCGTGAAGGCATACtt           >  1:1888207/1‑150 (MQ=255)
                                                                                                                                              tttCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCa        <  1:1000390/150‑1 (MQ=255)
                                                                                                                                                 cGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGa     <  2:4139/150‑1 (MQ=255)
                                                                                                                                                  gCCCCGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAg    >  1:1135545/1‑150 (MQ=255)
                                                                                                                                                  gCCCCGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCGGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAg    >  1:1135541/1‑150 (MQ=255)
                                                                                                                                                    ccTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTg  >  2:1462868/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTAGTAAGCCTGGAAGCTAATCACTAAGAGTATCACCAGTATGATGACGTGCTTCATCATAACCCTTTCCTTATTAAAAGCCCTCTTCTCCGGGAGAGGCTTTCCCGTTTCAGCGTCCCGCTGAAATCATCGGCTTACCTCCTTTCGCCCTGAATGCAGTCTATCGCTAACGCGTGAATAAAATAGTTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTG  >  NC_000913/2153349‑2153646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: