Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,555 A→T 8.2% intergenic (‑86/+95) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,5550AT 8.2% 406.2 / 2.7 139intergenic (‑86/+95)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base A (60/60);  new base T (4/11);  total (64/75)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

GTAAACCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAA  >  NC_000913/2460406‑2460704
                                                                                                                                                      |                                                                                                                                                     
gtaAACCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGaaaa                                                                                                                                                       <  2:1093588/150‑1 (MQ=255)
gtaAACCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGaaaa                                                                                                                                                       <  1:1964430/150‑1 (MQ=255)
    aCCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGa                                                                                                                                                   >  2:666874/1‑150 (MQ=255)
    aCCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGa                                                                                                                                                   >  2:522149/1‑150 (MQ=255)
    aCCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGa                                                                                                                                                   >  2:1685398/1‑150 (MQ=255)
          aaCAATGGCGATCCGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTgg                                                                                                                                             >  1:785675/1‑150 (MQ=255)
          aaCAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTgg                                                                                                                                             >  1:876962/1‑150 (MQ=255)
          aaCAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTgg                                                                                                                                             >  1:785680/1‑150 (MQ=255)
           aCAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTggg                                                                                                                                            <  1:230356/150‑1 (MQ=255)
           aCAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACAATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTggg                                                                                                                                            <  1:230385/150‑1 (MQ=255)
                 gCGATACGATCGCCCGGGCGGGTCACCAGCGGTAAAACCTGCCCCAAACCACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTAt                                                                                                                                      <  1:2635524/150‑1 (MQ=255)
                  cGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATg                                                                                                                                     >  1:1219063/1‑150 (MQ=255)
                    aTACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGac                                                                                                                                   >  2:945635/1‑150 (MQ=255)
                       cGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCATTTAATAAAATAAATATAGTGGTCTTACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGAcaca                                                                                                                                <  1:2076213/150‑1 (MQ=255)
                         aTCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACaga                                                                                                                              >  2:2714775/1‑150 (MQ=255)
                          tCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagag                                                                                                                             <  2:1944680/150‑1 (MQ=255)
                           cGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATACAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagaga                                                                                                                            <  1:457669/150‑1 (MQ=255)
                           cGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagaga                                                                                                                            <  1:1957752/150‑1 (MQ=255)
                           cGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagaga                                                                                                                            <  2:2686917/150‑1 (MQ=255)
                           cGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagaga                                                                                                                            <  1:457674/150‑1 (MQ=255)
                           cGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagaga                                                                                                                            <  2:319233/150‑1 (MQ=255)
                           cGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACagaga                                                                                                                            <  1:1099634/150‑1 (MQ=255)
                              ccTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaa                                                                                                                         >  2:1599055/1‑150 (MQ=255)
                              ccTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaa                                                                                                                         <  1:650812/150‑1 (MQ=255)
                              ccTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGAAACAGAGAGaa                                                                                                                         >  2:2591374/1‑150 (MQ=255)
                              ccTGGCGGGTAACCAGCGGTAAAACCTCACCCATAACACTCCCCTCTAAAAAAATAAATAAACTCGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGaa                                                                                                                         <  1:1725223/150‑1 (MQ=255)
                                tGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGTAAGTGGGAGCTATGACACAGAGAGaaaa                                                                                                                       <  2:624358/150‑1 (MQ=255)
                                 ggCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaag                                                                                                                      >  1:2647318/1‑150 (MQ=255)
                                  gCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaaga                                                                                                                     <  2:834406/150‑1 (MQ=255)
                                  gCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaaga                                                                                                                     <  2:488210/150‑1 (MQ=255)
                                           cAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaa                                                                                                            >  2:2100081/1‑150 (MQ=255)
                                           aagagttAAAAACTGACCCATAACACTCCCCTGTAAAAAAAAAAATAAAGTGGTCTGACCTGATCAAAGACTTAACCATTTTTTTACATTTTGACAAGTGGAGAAAAGGGAAAGTGGGAGCAATGAAACAGAGAGAAAAGTAGAAGagaa                                                                                                            >  2:1844025/7‑150 (MQ=255)
                                             gCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTTGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaa                                                                                                          <  2:18032/150‑1 (MQ=255)
                                              cGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGTTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAGAAGCAGAAGagaaaag                                                                                                         >  2:1750603/1‑150 (MQ=255)
                                                  aaaaGCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACGTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGATAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                     <  2:2531293/150‑1 (MQ=255)
                                                  aaaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                     <  1:2110002/150‑1 (MQ=255)
                                                  aaaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                     <  2:1133568/150‑1 (MQ=255)
                                                   aaaCCTGACCTATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAATGGGAAAGTGGGAGCTATGACACAGAGAGAATAGAAGAAGAGAAATGAATAc                                                                                                    >  1:2054945/1‑150 (MQ=255)
                                                   aaaCCTGACCTATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAAc                                                                                                    >  1:1703257/1‑150 (MQ=255)
                                                       cttaacaaTAACACTCCCCTTTAAAAAAATAAATAAAGTGGTCTTACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGTAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGccc                                                                                                <  2:2699928/143‑1 (MQ=255)
                                                       cTGACCCATAACACTCCCCTTTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGATATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGccc                                                                                                <  2:2234371/150‑1 (MQ=255)
                                                             cATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCa                                                                                          >  1:1705743/1‑150 (MQ=255)
                                                             cATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCa                                                                                          >  1:591510/1‑150 (MQ=255)
                                                             cATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCa                                                                                          >  1:2062470/1‑150 (MQ=255)
                                                                  cacTCCCTTTTAAAAAAATAAATAACGTGGTCTGACCTGATAATTGTCTTAACCTTTTTTTTAAATTTCGCCATGGGGATAAATGGGAATGTGGTTGCTATGACACAGCGAGAAACTAAGAAGAGAAACCAAAACGCCCCTGCCAGCAAc                                                                                     <  2:2564069/150‑1 (MQ=255)
                                                                         cTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGGGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgg                                                                              >  1:865188/1‑150 (MQ=255)
                                                                         cTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGACGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgg                                                                              >  1:2457374/1‑150 (MQ=255)
                                                                         cTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGACAAGAAAACGCCCCTGCCAGCAACTGACAgg                                                                              >  1:1499105/1‑150 (MQ=255)
                                                                         cTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgg                                                                              >  1:1122727/1‑150 (MQ=255)
                                                                         cTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgg                                                                              >  1:2428816/1‑150 (MQ=255)
                                                                         cTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgg                                                                              >  2:1849310/1‑150 (MQ=255)
                                                                          tttAAAAAAATAAATACAGTGTTCTGACCTGATCATTGTCTTAACCATTTTTTTAATTTTAGCCAAGTGTAGAAAAGGTAATGTGGGAGATTTGACACATAGAGAAAAGAAGACGCGAAAACAAAACGCCCCTGCCAGCAACTGACAggg                                                                             <  2:1301028/148‑1 (MQ=255)
                                                                          tttAAAAAAATAAATAAAGTGGTCTGACCTGATCATTTTCTTAACAATTTTTTTAAATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGACAAAAGAAGACGAGAAAACAAAACGCCCCTGCCAGCAACTGACAggg                                                                             <  2:1238723/148‑1 (MQ=255)
                                                                          tGTAAAAAAATAAATAAAGTGGTCTGACATTATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAATGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAACACGCCCCTGCCAGCAACTGACAggg                                                                             <  2:613470/150‑1 (MQ=255)
                                                                          ggaaaaaaaaGACATACCGTGGTCTGACCTGATAATAGTCTTAACCTTTTTTTTACATTTCGCCAAGTGGAGAAAAGGGAATGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAggg                                                                             <  2:2668468/147‑1 (MQ=255)
                                                                            tAAAAAAATAAATAACGTGGTCTGACCGGACCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAATGTGGGAGCTATGACACCGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                           <  2:633833/150‑1 (MQ=255)
                                                                            tAAAAAAATAAATAAAGTGGTCTGACCTTATAATAGTCTTAACAATTTTTTTAAATTTAGCCAAGTGGAGAAATGGGAATGTGGGAGCTATCACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                           <  2:536397/150‑1 (MQ=255)
                                                                            tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                           >  2:405130/1‑150 (MQ=255)
                                                                            tAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                           >  2:405117/1‑150 (MQ=255)
                                                                             aaaaaaaTAAAAGAACGTGGTGTTACCGTAAAATAGTCTTAACCATTTTTTTACATTGAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGACGCGAAACTAACACGCCCCTGCCAGCAACTGACAGGGGc                                                                           <  2:2398005/150‑1 (MQ=255)
                                                                              aaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCGAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGATAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgt                                                                         >  2:214410/1‑150 (MQ=255)
                                                                              aaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGTAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgt                                                                         >  2:1992931/1‑150 (MQ=255)
                                                                              aaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgt                                                                         >  2:2311250/1‑150 (MQ=255)
                                                                                 aaataaatAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAATGGGAAAGTGGGAGCTATGACACAGAGAGAAATGAAGAAGAGAAAAGAAATCGCCCCTGCCAGCAACTGACAGGGGcgtacg                                                                      >  2:2469371/1‑150 (MQ=255)
                                                                                       aatacaGTGTTCTGTCGTTATTATAGTCTTAACCTTTTTTTTACATTTTGCAAAGTGGAGACATGGGAAAGTGGGAGCTTCGACACAGTGAGAAAAGAATAACAGAAAAGAAAACGCCCCTGCCTTCAACTGACTGGGGCGTACGTAccc                                                                 <  2:1365009/150‑1 (MQ=255)
                                                                                         tcccgTGGTCGGCCCTGATCATAGTCTTAACCTTTTTTTTACATTTCGCCCAGGGGAGAAACGGGAAAGTGGGAGCTAGGACCCCGAGAGAAAAGACGCCGAGAAAAGACACCGCCCCGGCCAGCAACTGACAGGGGCGTACGTACCCga                                                               <  2:2648253/146‑1 (MQ=255)
                                                                                         tcaaGTGGTCTGACCTGACCATAGTCTTAACCATTTTGTTACATTCAGCCACGGGGATAAACGGGACAGTGGGCGCTATGACCCAGAGAGAAAACAAGAACAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCga                                                               <  2:1290526/148‑1 (MQ=255)
                                                                                         taaaGTGTTCTGACCTGATAATAGTCTTAACAATTTTTTTACATTTAGCCAAGTGTTTAAATGGTAAAGTGGGAGCTATGACACAGAGAGAAACGACGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCga                                                               <  2:815748/150‑1 (MQ=255)
                                                                                         taaaGTGGTCTGACCTGATCATAGTCTTACCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACCCAGAGAGAAAAGAAGAAGAGAAACGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCga                                                               <  2:2504602/150‑1 (MQ=255)
                                                                                         taaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGTGAAATGGGAAAGTGGGAGCTATGACACAGAGCGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCga                                                               <  2:2224279/150‑1 (MQ=255)
                                                                                                   tGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAATGTGGGAGCTATGACACAGTGACAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTaa                                                     <  2:430030/150‑1 (MQ=255)
                                                                                                   tGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTaa                                                     >  2:2414540/1‑150 (MQ=255)
                                                                                                   tGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTaa                                                     >  2:944051/1‑150 (MQ=255)
                                                                                                     aCCTGATCATATTCTTAACAATTTTTTTAAATTTTGCCATGTGGAGAATAGGGAAAGTGGTAGCTTGGACACAGAGAGAAATGAATAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACg                                                   <  2:1741004/150‑1 (MQ=255)
                                                                                                     aCCTGATCATAGTCTTAACCATTTTTTTACATTTCGCCAAGGGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACg                                                   <  2:1576346/150‑1 (MQ=255)
                                                                                                       cTGATCTTATTATTAAAATTTTTTTTAATTTTCGCCACGTGTATAAATGGTAATTTGGTAGCTATCAAACTTATAAAAATTACTAAGATAAAATAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                 <  1:1059481/150‑1 (MQ=255)
                                                                                                       cTGATCATTGTCTTACCCATTTTTTAACATTTAGCCACGTGGACAAACGGTAATGTGGTAGCTATGACACAGACACAAAATAATAAGAGAAAACAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                 <  2:715954/150‑1 (MQ=255)
                                                                                                       cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAACGACGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                 <  2:461418/150‑1 (MQ=255)
                                                                                                          aTCAAAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAc                                              >  1:101665/1‑150 (MQ=255)
                                                                                                             aTAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                           >  2:2334642/1‑150 (MQ=255)
                                                                                                             aTAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGTGTACGTACCCGAGAGGAATTAACGCAGACCCAg                                          >  2:1111270/1‑150 (MQ=255)
                                                                                                             aTAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGTGTACGTACCCGAGAGGAATTAACGCAGACCCAg                                          >  2:1662782/1‑150 (MQ=255)
                                                                                                               attCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAATGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCGGc                                         <  2:1368116/148‑1 (MQ=255)
                                                                                                                       ccATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaagaagaag                                                                                                                >  1:1546975/1‑71 (MQ=255)
                                                                                                                       ccATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAaagaagaag                                                                                                                <  2:1546975/71‑1 (MQ=255)
                                                                                                                       cattttttttAAATTTAGCCATGTGTATAAATGGCAATGTGGTTGCTATGACACATATATAAAATATTAAGAGAAAATAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                 <  1:1317767/147‑1 (MQ=255)
                                                                                                                       caaTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                 <  2:1156749/148‑1 (MQ=255)
                                                                                                                       caaTTTTTTTAATTTTAGCCATGTGGAGAAATGGTAAAGTGGGAGCTTTGACACATAGAGAAAAGAATACGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                 <  2:2117357/148‑1 (MQ=255)
                                                                                                                       aattttttttAATTTTAGCCATGTGGATAAATGGCAATGGGGGTGATATCACACAGATAGAAAAGAAGAATACAAAACAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                 <  1:67318/147‑1 (MQ=255)
                                                                                                                         aTTTTTTTACATTTAGCCAAGTGGAGAAATGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGTAGATGAGATTTGAATACGCCCCTGCGAGCAACTGTCGGGGGCGTGCGTAGCCGAGAGGGATTAACGCAGAGCCAGCTGGAAAATcg                               >  2:2594406/1‑149 (MQ=255)
                                                                                                                         aTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAACATCa                               >  2:858753/1‑150 (MQ=255)
                                                                                                                         aTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                               >  1:2450247/1‑150 (MQ=255)
                                                                                                                         aTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                               >  2:861140/1‑150 (MQ=255)
                                                                                                                          tttttttACATTTAGCCAAGTGGAGAAAAGGGAGAGTGGGAACTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                              >  2:2147605/1‑150 (MQ=255)
                                                                                                                          tttttttACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGCGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                              >  2:2147568/1‑150 (MQ=255)
                                                                                                                          tttttttACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAg                              >  2:558733/1‑150 (MQ=255)
                                                                                                                            tttttACTTTTCGCCATGTTGATAAAAGGTAATGTGGTAGATATCACACAGATAGAAAACAACACCACAAACGAACCCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCa                            <  1:1352615/150‑1 (MQ=255)
                                                                                                                                                tataaaaGGGAATGTGGGAGCTATGACACAGATAGAAAACAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa        <  1:651828/147‑1 (MQ=255)
                                                                                                                                                gataaatGGGATTGTGGGAGCTATTACACATAGATAAAAGAATAAGACAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa        <  1:666874/147‑1 (MQ=255)
                                                                                                                                                      aGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa  <  2:1546244/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GTAAACCGCTAACAATGGCGATACGATCGCCCTGGCGGGTAACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAA  >  NC_000913/2460406‑2460704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: