Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,427 C→A 18.4% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4270CA18.4% 303.5 / 14.1 122Q433K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (25/70);  new base A (19/8);  total (44/78)
Fisher's exact test for biased strand distribution p-value = 6.18e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATG  >  NC_000913/2470280‑2470574
                                                                                                                                                   |                                                                                                                                                    
gtagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaa                                                                                                                                                    <  2:1517509/150‑1 (MQ=255)
 tagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaaa                                                                                                                                                   <  1:277494/150‑1 (MQ=255)
    taTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGaa                                                                                                                                                <  1:1244184/150‑1 (MQ=255)
      tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCTTTCGACCTTTGCTTATAAATAACATTCGTTTTTTTAATTTATAAAATAATTaaaaaaaaa                                                                                                                                              >  1:883821/1‑149 (MQ=255)
      tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAATTAAAAGaaaa                                                                                                                                              >  1:471473/1‑149 (MQ=255)
        tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                                                                                            <  2:876659/150‑1 (MQ=255)
         ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCaa                                                                                                                                           >  1:1509730/1‑150 (MQ=255)
                gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACGTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                    <  2:974356/150‑1 (MQ=255)
                gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                    <  2:568670/150‑1 (MQ=255)
                gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                    <  1:2343549/150‑1 (MQ=255)
                 aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                   <  2:615948/150‑1 (MQ=255)
                 aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                   <  2:2042933/150‑1 (MQ=255)
                 aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                   <  2:2042914/150‑1 (MQ=255)
                 aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                   <  2:1827632/150‑1 (MQ=255)
                 aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                   <  2:1827630/150‑1 (MQ=255)
                    atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                               <  1:1150894/150‑1 (MQ=255)
                    atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                               <  2:1217258/150‑1 (MQ=255)
                    atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                               <  1:2689263/150‑1 (MQ=255)
                    atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                               <  2:1289055/150‑1 (MQ=255)
                    atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                               <  1:2495246/150‑1 (MQ=255)
                     tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                              <  2:683766/150‑1 (MQ=255)
                     tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAAAAAAAAATACAATATCgct                                                                                                                              >  1:2142197/1‑148 (MQ=255)
                     tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAAAAAAGACAAAAGCGAt                                                                                                                              >  1:2470973/1‑150 (MQ=255)
                       tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAAAACAAAAAAAACAAAACAAAAGccaagt                                                                                                                            >  2:2340037/1‑145 (MQ=255)
                         aaTTTTATGTGTCATTGTTCGGGGAAGGATATAGAGAATTAAGCAAGCATTTATTTGGAATGTATTTTTCTTTCGACCATTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaa                                                                                                                          <  1:1854021/150‑1 (MQ=255)
                             ttATGTGTCATTTTTCGGGGAAGGAGATAGAGATTTCAGCAAGCATTTCTTTGGCATGGGGTGTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAGATAAGTCTAAGAAGACAATACAATTGCGAAGTGAAGCa                                                                                                                      >  2:1534749/1‑150 (MQ=255)
                              tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                                                                                                     <  2:1569581/150‑1 (MQ=255)
                                 gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGGATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGTTGTAAAGCTCTaa                                                                                                                  <  2:731000/150‑1 (MQ=255)
                                  tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTTTAAAATAATTAAAAGAAATAAAGAAAAAAACCAAGTAAAGCACAAAg                                                                                                                 >  1:2149683/1‑150 (MQ=255)
                                  tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                                                                                                 <  1:1560490/150‑1 (MQ=255)
                                  tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAg                                                                                                                 >  2:1467923/1‑150 (MQ=255)
                                  tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAAATCAAAGCAAACAACACAAAAGCGATGTAAAGCACGAAg                                                                                                                 >  2:719964/1‑150 (MQ=255)
                                    tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                               <  1:2672429/150‑1 (MQ=255)
                                    tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                               <  1:429056/150‑1 (MQ=255)
                                    tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                               <  1:428990/150‑1 (MQ=255)
                                    tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                               <  1:428985/150‑1 (MQ=255)
                                      aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTa                                                                                                             >  2:2216876/1‑150 (MQ=255)
                                      aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAATAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTa                                                                                                             >  2:901358/1‑150 (MQ=255)
                                       tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGAAAAAAGCGATGTAAATCACTAAGTTTaa                                                                                                            >  2:1382147/1‑150 (MQ=255)
                                       tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAAAAAATAAAAGAAAAAAAAAAAAAATCCATTTAAAGCACGAAGATTat                                                                                                            >  2:1173736/1‑149 (MQ=255)
                                       tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACATTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATACAAAGAAAAAAACACAAAAGCGAGGTAAAGCACAAAGTTTaa                                                                                                            >  2:1173737/1‑150 (MQ=255)
                                        ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                           <  2:308201/150‑1 (MQ=255)
                                        ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                           <  1:1199075/150‑1 (MQ=255)
                                        ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                           <  1:1859374/150‑1 (MQ=255)
                                        ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                           <  2:2042314/150‑1 (MQ=255)
                                           tCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTg                                                                                                        <  2:11966/150‑1 (MQ=255)
                                           tCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTg                                                                                                        <  2:2079887/150‑1 (MQ=255)
                                             ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                      <  2:392112/150‑1 (MQ=255)
                                             ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAACAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                      <  2:159780/150‑1 (MQ=255)
                                             ggggAAGGATATAGAGATTGAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                      <  2:1948295/150‑1 (MQ=255)
                                                  aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCa                                                                                                 <  1:2519512/150‑1 (MQ=255)
                                                           agaTTTAAGCAAGCATTTATTTGGAATGTAATTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcg                                                                                        <  1:687429/150‑1 (MQ=255)
                                                             aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcg                                                                                      >  2:2283436/1‑150 (MQ=255)
                                                             aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAAAAAAAAAAAAAGCGATGTAAATCACAAAGTTTAAATTTCGCGCCAAAAATTgcgcg                                                                                      >  2:1286770/1‑150 (MQ=255)
                                                              tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAAAAGAGATGTAAAGCACGAAGTTTAAATTTAGAGCCAATCATGgcgcgc                                                                                     >  2:1827382/1‑150 (MQ=255)
                                                                 aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAATAAATAAAAAATAGATTTAAATCAAAAAGTTTAAATTTCGCGCCAAACAGGGGGCGcacc                                                                                  >  1:1417953/1‑149 (MQ=255)
                                                                    caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAATACAAAAGCGATGTAAAGCACTAAGTTTTAATTTCGCGCCAATCAAGGCGCGCACACGc                                                                               >  2:522623/1‑150 (MQ=255)
                                                                    caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGAAAAAATCGATGTAAATCACAAATTTTAAATTTATCGCCACTCAAGGCGCGCACaaac                                                                               >  1:2024111/1‑148 (MQ=255)
                                                                    caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAATAAATAAAGAAAAAAGCGATTTAAATAACTAAATTTAAATTTAGCGCCAAAAAAGGCGCGCACAAGc                                                                               >  2:522613/1‑150 (MQ=255)
                                                                    caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAATCAAAAAAATAGACGTTGAAAGCCAGAAGTTTAAATTTCGCGGCCATAATGGGGCGCACAAGc                                                                               >  1:1391803/1‑150 (MQ=255)
                                                                        caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAATCACTAAGTTTAAATTGCGCGCCAATCAAGGCGCGCACAATCtaaa                                                                           >  2:1458212/1‑148 (MQ=255)
                                                                        caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAATCAAGACAATAGCGATGTAAAGCAATAAGTTTAAATTTCGCGCCAATAATTGCGCGCACAAGCtata                                                                           >  2:926596/1‑150 (MQ=255)
                                                                          tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAAAAAATAAAAAAAAAAAACAAAAAATCGAAGTAAAGAACAAAGGTTAAATTTAACGCCCAACATGGCCCCCACAAGATaaaaa                                                                         >  1:1973362/1‑149 (MQ=255)
                                                                              tttGTATTGTATTTTTCTTTCGACCTTTGATTATACATAACATTCGTTTTTTTATTTTATAAAATAATTCAAATAAATCAAGAAAATCGCGATGTAAAGCAATAAGTTTAAATTGCGCGCCAATCAGGGCGCGCACAAGCTATAATACCa                                                                     <  1:2041376/150‑1 (MQ=255)
                                                                              tttGGAATTTTTTTTTATTTCTAACTTTGCTTATACATAAATGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATTGCGATTAAAAGCACTATGTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCa                                                                     <  1:2041190/150‑1 (MQ=255)
                                                                                     tttttttttCGTTCGACGTTTGCTTATACATAACTGTGGTTTTTTTATTTTATAAAATATTTCAAAGAAATCAAGACATTAGCGATGTAACGCACTATGTTTAATTTGCGCGCCAATCTGGGCGCGCACAAGCTATAATACCAACCTAAt                                                              <  1:1086569/146‑1 (MQ=255)
                                                                                     tttttttttCGTTCGACATTTGATTATACATAACTTTTTTTTTTTTATTTTATAAAATAATTCAAATAAATAAATACATTAGCTATTTAAAGCAATAATTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAAt                                                              <  1:884422/146‑1 (MQ=255)
                                                                                     tttATTTTTCTTTCTAACTTTTCTTATAAATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATTTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                              <  1:1803199/148‑1 (MQ=255)
                                                                                     tGTATTTTTCGTTCTACATTTTCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                              <  1:1071024/150‑1 (MQ=255)
                                                                                     tGTATTTTTCGTTCGACCTTTGATTATAAATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCTATGTAAATCACTATGTTTAAATTGCGCGCCATTCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                              <  1:78294/150‑1 (MQ=255)
                                                                                      ttATTTTTCGTTCTACATTTGCTTATAAATAACAGTCTTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGAAAATAGCTTTTTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                             <  1:1259764/149‑1 (MQ=255)
                                                                                      gTATTTTTGGTTCGACCTTTTCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAACACAATCGCGATGTAAAGCACTATTTTTAAATTGCGCGCCAATCTGGGCGCGCACAAGCTATAATACCAACCTAAtt                                                             <  1:1259767/150‑1 (MQ=255)
                                                                                        aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                           <  2:134687/150‑1 (MQ=255)
                                                                                        aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                           <  2:478554/150‑1 (MQ=255)
                                                                                        aTTTTTCGTTCGACCTTTGATTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGAAATTAGCTATTTAAAGAACTAATTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                           <  1:1361823/150‑1 (MQ=255)
                                                                                        aTTTTTCATTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                           <  2:390136/150‑1 (MQ=255)
                                                                                         tttttCTTTCGACATTTGCTTATACATAACAGTCTTTTTTTTATTTTATAAAATAATTCAAAGAAATCAAGACAATATCTATTTAAAGAACTAAGTTTAAATGGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                          <  1:534803/150‑1 (MQ=255)
                                                                                          ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                         <  2:1509730/150‑1 (MQ=255)
                                                                                                       tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                            <  1:827912/150‑1 (MQ=255)
                                                                                                       tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                            <  1:828015/150‑1 (MQ=255)
                                                                                                           cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                        <  2:1468182/150‑1 (MQ=255)
                                                                                                           cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                        <  2:2629096/150‑1 (MQ=255)
                                                                                                                   tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGTAATAAAGACAATATCGATGTAAAGCACTAAGTTTAAATTTCGCGCCTATCATGGCGCGCACAAGCGATAATTCCAACCTAATTTCTCCTCCTCTTTGGGTGACTATATGTcc                                >  2:1796011/1‑150 (MQ=255)
                                                                                                                   tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAAGCGAAGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCAAATTTCTCCTCCTCATAGAGTGACGATATCTcc                                >  2:2003520/1‑150 (MQ=255)
                                                                                                                    aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                               <  2:1107573/150‑1 (MQ=255)
                                                                                                                    aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                               <  2:2072498/150‑1 (MQ=255)
                                                                                                                          cGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGa                         <  2:2470973/150‑1 (MQ=255)
                                                                                                                            tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                       >  1:1893131/1‑150 (MQ=255)
                                                                                                                                tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                   <  2:1933031/150‑1 (MQ=255)
                                                                                                                                tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                   >  2:1987993/1‑150 (MQ=255)
                                                                                                                                tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                   >  2:1475428/1‑150 (MQ=255)
                                                                                                                                tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                   <  1:698886/150‑1 (MQ=255)
                                                                                                                                tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACAATATCTCCTGATAGAATTGCg                   >  1:2544947/1‑150 (MQ=255)
                                                                                                                                tttAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                   <  1:1162959/150‑1 (MQ=255)
                                                                                                                                        ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc           >  1:2106914/1‑150 (MQ=255)
                                                                                                                                        ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc           >  1:27784/1‑150 (MQ=255)
                                                                                                                                         taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt          >  1:2032941/1‑150 (MQ=255)
                                                                                                                                         taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGAACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt          >  2:723261/1‑150 (MQ=255)
                                                                                                                                               aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa    <  1:569658/150‑1 (MQ=255)
                                                                                                                                                 ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg  <  1:1264054/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATG  >  NC_000913/2470280‑2470574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: