Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,668,982 T→C 6.0% intergenic (‑86/‑50) hcaR ← / → hcaE hca operon transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,668,9820TC 6.0% 431.6 / 13.1 133intergenic (‑86/‑50)hcaR/hcaEhca operon transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base T (80/45);  new base C (4/4);  total (84/49)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 134 total aligned reads displayed.

CCGCACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGACCCGGATATTTACCA  >  NC_000913/2668833‑2669126
                                                                                                                                                     |                                                                                                                                                
      gggTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAACTTAACTATTTCACATATTAGCAACCAACCAGCACCATCCTTATGGcaca                                                                                                                                            >  1:866694/1‑150 (MQ=255)
      gggTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGcaca                                                                                                                                            >  1:1238879/1‑150 (MQ=255)
      gggTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGcaca                                                                                                                                            >  1:8486/1‑150 (MQ=255)
           aaGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAAt                                                                                                                                       >  1:2462901/1‑150 (MQ=255)
           aaGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAAt                                                                                                                                       >  1:2102249/1‑150 (MQ=255)
            aGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATa                                                                                                                                      >  2:1361637/1‑150 (MQ=255)
              ttCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGa                                                                                                                                    >  1:1597109/1‑150 (MQ=255)
               tCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGaa                                                                                                                                   <  1:1504123/150‑1 (MQ=255)
               tCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGaa                                                                                                                                   <  1:1829304/150‑1 (MQ=255)
                  gTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTATCTATTTCACATAGTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGt                                                                                                                                >  2:2064769/1‑150 (MQ=255)
                  gTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGt                                                                                                                                <  2:1796603/150‑1 (MQ=255)
                  gTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGt                                                                                                                                >  2:2711800/1‑150 (MQ=255)
                  gTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGt                                                                                                                                >  1:20530/1‑150 (MQ=255)
                  gTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGt                                                                                                                                >  2:2064808/1‑150 (MQ=255)
                  gTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGt                                                                                                                                >  1:2562335/1‑150 (MQ=255)
                      cTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAAt                                                                                                                            >  2:2006887/1‑150 (MQ=255)
                       tGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATa                                                                                                                           >  1:2202498/1‑150 (MQ=255)
                       tGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATa                                                                                                                           >  1:577243/1‑150 (MQ=255)
                       tGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATa                                                                                                                           >  2:1198886/1‑150 (MQ=255)
                          gcCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACat                                                                                                                        >  2:1802837/1‑150 (MQ=255)
                          gcCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACat                                                                                                                        >  2:882009/1‑150 (MQ=255)
                           ccACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTCACTATTTCACATAGTAGCAACCAACCAGCAACATCCTTACGGCACAAAAATAGACGGTCAATACatc                                                                                                                       >  2:1732561/1‑150 (MQ=255)
                           ccACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTACGGCACAAAAATAGAAGGTCAATACatc                                                                                                                       >  2:1732585/1‑150 (MQ=255)
                           ccACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTACGGCACAAAAATAGAAGGTCAATACatc                                                                                                                       >  2:1324606/1‑150 (MQ=255)
                           ccACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTACGGCACAAAAATAGAAGGTCAATACatc                                                                                                                       >  2:1028237/1‑150 (MQ=255)
                                 cgacgaAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACatcttatct                                                                                                                 >  1:584489/1‑150 (MQ=255)
                                 cgacgaAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACatcttatct                                                                                                                 >  2:2638013/1‑150 (MQ=255)
                                      aaaTAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAg                                                                                                            >  2:431404/1‑150 (MQ=255)
                                                aaTGCCGTAGTTCCATCACCTTCCCGTTGTTATCGAAAAAACGTCTCAGCTGGTAGATATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTaaaaaa                                                                                                  <  2:1949017/150‑1 (MQ=255)
                                                 aTGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTAAATACATCTTATCTTTCAGGATTAAAAAat                                                                                                 <  1:192856/150‑1 (MQ=255)
                                                  tGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAata                                                                                                <  1:2013460/150‑1 (MQ=255)
                                                  tGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAata                                                                                                <  1:1248274/150‑1 (MQ=255)
                                                  tGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAata                                                                                                <  1:1248597/150‑1 (MQ=255)
                                                    ccGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATg                                                                                              >  1:1620976/1‑150 (MQ=255)
                                                           tCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCacac                                                                                       <  2:345105/150‑1 (MQ=255)
                                                            ccATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACAcc                                                                                      >  1:323574/1‑150 (MQ=255)
                                                            ccATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACAcc                                                                                      >  1:304869/1‑150 (MQ=255)
                                                            ccATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACAcc                                                                                      >  1:304812/1‑150 (MQ=255)
                                                            ccATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACAcc                                                                                      >  1:136433/1‑150 (MQ=255)
                                                            ccATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACAcc                                                                                      >  1:2525653/1‑150 (MQ=255)
                                                                    ttCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGAtt                                                                              <  1:1730971/150‑1 (MQ=255)
                                                                               aTCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTAc                                                                   <  2:590158/150‑1 (MQ=255)
                                                                               aTCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTAc                                                                   <  2:1634240/150‑1 (MQ=255)
                                                                                tCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTAcc                                                                  >  1:185873/1‑150 (MQ=255)
                                                                                  gAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATTACAACACCCTCAGATTTGAACATTTACCaa                                                                >  2:1913777/1‑150 (MQ=255)
                                                                                  gAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCaa                                                                >  1:2268020/1‑150 (MQ=255)
                                                                                   aaaaaaCGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAAc                                                               <  1:1219398/150‑1 (MQ=255)
                                                                                   aaaaaaCGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAAc                                                               >  1:563613/1‑150 (MQ=255)
                                                                                    aaaaaCGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACt                                                              <  1:1466316/150‑1 (MQ=255)
                                                                                      aaaCGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGa                                                            <  1:678383/150‑1 (MQ=255)
                                                                                        aCGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGAtt                                                          >  2:151004/1‑150 (MQ=255)
                                                                                        aCGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGAtt                                                          >  2:960598/1‑150 (MQ=255)
                                                                                          ctctcAGCTGGTAGAAATTAACTAAATCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATACAACCTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGa                                                        <  2:1449755/149‑1 (MQ=255)
                                                                                           tctcAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGac                                                       >  2:894198/1‑150 (MQ=255)
                                                                                            ctcAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGaca                                                      <  2:2708837/150‑1 (MQ=255)
                                                                                            ctcAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGaca                                                      <  2:1396056/150‑1 (MQ=255)
                                                                                             tcAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGacac                                                     >  1:2071859/1‑150 (MQ=255)
                                                                                             tcAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGacac                                                     >  1:2160204/1‑150 (MQ=255)
                                                                                             tcAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGacac                                                     >  1:2160201/1‑150 (MQ=255)
                                                                                             tcAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGacac                                                     >  1:1378715/1‑150 (MQ=255)
                                                                                             tcAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGacac                                                     >  1:1050962/1‑150 (MQ=255)
                                                                                               aGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACAccc                                                   >  1:2504260/1‑150 (MQ=255)
                                                                                               aGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACAccc                                                   >  1:2203815/1‑150 (MQ=255)
                                                                                               aGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACAccc                                                   >  1:1454703/1‑150 (MQ=255)
                                                                                                   ggTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCaaaa                                               >  1:1593738/1‑150 (MQ=255)
                                                                                                   ggTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCaaaa                                               >  1:1064785/1‑150 (MQ=255)
                                                                                                         aaTTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCg                                         <  1:1839194/150‑1 (MQ=255)
                                                                                                          aTTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCgg                                        <  1:2613523/150‑1 (MQ=255)
                                                                                                           ttAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCggg                                       >  1:319747/1‑150 (MQ=255)
                                                                                                           ttAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCggg                                       >  2:488155/1‑150 (MQ=255)
                                                                                                           ttAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCggg                                       >  2:521938/1‑150 (MQ=255)
                                                                                                           ttAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCggg                                       >  1:319919/1‑150 (MQ=255)
                                                                                                            tAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGt                                      <  1:1198886/150‑1 (MQ=255)
                                                                                                              aCTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCa                                    >  2:2408505/1‑150 (MQ=255)
                                                                                                                 aTTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTc                                 <  1:1283579/150‑1 (MQ=255)
                                                                                                                 aTTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTc                                 <  1:1366943/150‑1 (MQ=255)
                                                                                                                    tCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCcgc                              <  2:802884/150‑1 (MQ=255)
                                                                                                                    tCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCcgc                              <  2:346969/150‑1 (MQ=255)
                                                                                                                         tatTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTAtt                         <  1:1771238/150‑1 (MQ=255)
                                                                                                                          atTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTAttt                        >  2:1102630/1‑150 (MQ=255)
                                                                                                                           ttAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTta                       >  2:9668/1‑150 (MQ=255)
                                                                                                                           ttAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTta                       >  2:1945322/1‑150 (MQ=255)
                                                                                                                             aGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTtata                     <  1:2064769/150‑1 (MQ=255)
                                                                                                                             aGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTtata                     <  2:20530/150‑1 (MQ=255)
                                                                                                                             aGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTtata                     <  2:2562335/150‑1 (MQ=255)
                                                                                                                             aGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTtata                     <  1:2064808/150‑1 (MQ=255)
                                                                                                                             aGCAACCAACCAGCAACATCCTTACGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTtata                     <  1:1028237/150‑1 (MQ=255)
                                                                                                                             aGCAACCAACCAGCAACATCCTTACGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTtata                     <  1:1324606/150‑1 (MQ=255)
                                                                                                                             aGCAACCAACCAGCAACATCCTTACGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTtata                     <  1:1732561/150‑1 (MQ=255)
                                                                                                                              gCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATAc                    <  1:1280762/150‑1 (MQ=255)
                                                                                                                                aaccaaccAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCg                  >  1:125377/1‑150 (MQ=255)
                                                                                                                                  ccaaccaGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGAc                <  1:2006887/150‑1 (MQ=255)
                                                                                                                                   caaccaGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGAcc               >  1:1927994/1‑150 (MQ=255)
                                                                                                                                    acccaGCAACATCCTTATGGCACAAAAATAGACGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGAccc              <  1:623038/148‑1 (MQ=255)
                                                                                                                                    aaccaGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGAccc              <  1:155423/150‑1 (MQ=255)
                                                                                                                                    aaccaGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGAccc              >  2:1354970/1‑150 (MQ=255)
                                                                                                                                         gCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGACCCGGata         >  2:2487609/1‑150 (MQ=255)
                                                                                                                                           aaCATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGACCCGGATAtt       <  1:894198/150‑1 (MQ=255)
                                                                                                                                                ccTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGACCCGGATATTTACCa  >  1:2383628/1‑150 (MQ=255)
                                                                                                                                                ccTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGACCCGGATATTTACCa  >  1:647268/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CCGCACGGGTAAAGTTCAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAACCAGCAACATCCTTATGGCACAAAAATAGAAGGTCAATACATCTTATCTTTCAGGATTAAAAAATATGACCACACCCTCAGATTTGAACATTTACCAACTGATTGACACCCAAAATGGTCGGGTCACTCCGCGTATTTATACCGACCCGGATATTTACCA  >  NC_000913/2668833‑2669126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: