Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,817,774 A→T 6.2% intergenic (‑271/+10) yqaB ← / ← argQ fructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,817,7740AT 6.2% 548.4 / 7.2 166intergenic (‑271/+10)yqaB/argQfructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (70/83);  new base T (8/4);  total (79/87)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

TTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAG  >  NC_000913/2817626‑2817920
                                                                                                                                                    |                                                                                                                                                  
tttgttggAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCAGTGACGTTCGCCCTTCGGGGCGTTGGTGGGGGAACGTTATCGTTGGTGGTGGCTGGGGTTTATAGCGGTATGTGTTGGATTCCTTGTTTGGTGTTGGTTag                                                                                                                                                   >  2:1017999/7‑149 (MQ=255)
 ttGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACg                                                                                                                                                  <  2:509880/150‑1 (MQ=255)
     aGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGgaga                                                                                                                                              <  1:1413503/150‑1 (MQ=255)
       gAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGGTGGCGCAACGTTATACGTCCTGGTGCCAGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTTCGGAGAat                                                                                                                                            >  1:810125/1‑150 (MQ=25)
       gAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGGAACGTTATCCTTCCTGGTGCCTGCGGTTAATAACGCTATCTCTTCGGTACCTTCATTGGTGGAGGTTTCggtggat                                                                                                                                            >  1:810106/1‑145 (MQ=25)
       gAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGAGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTAACTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAat                                                                                                                                            >  1:2645982/1‑150 (MQ=255)
       gAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCGTCGGGCCGTTGCTGGCGCAACGATATCCTTCCTGGTGCCTGCGGGTAATAACGCTAACACTTCGATACCATCATTTCTGGGGGTTACGGAGAat                                                                                                                                            >  1:519895/1‑150 (MQ=25)
       gAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACCTTCGCCCAACGGGCCGTTGCTGGCGCAACGTTATCCTTCATGGTGCCTGCGGGTAATAACCCAATCTCGTCGATACCTTCATTGCTGAAGGTTACGGAGAat                                                                                                                                            >  2:713700/1‑150 (MQ=25)
       gAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACAGACGTTCGCCCTTCTGGCCGTTGCTTGCGCAACGTTATCCTTACTGGTGACTGCTGTTAATACCGAAATCTATTCGATACCTTCATTTCTGAAGGTTACGgagagt                                                                                                                                            >  2:1476175/1‑148 (MQ=25)
        aaaTTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGAGCAACGTTATCATTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATAACTTCATTGCTGAGGGTTACGGAGAata                                                                                                                                           >  2:2057426/1‑150 (MQ=255)
           ttttttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATTCCTTCATTGATGAAGGTTACGGAGATTATgg                                                                                                                                        <  2:2083347/150‑1 (MQ=255)
           ttttttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATgg                                                                                                                                        <  2:589774/150‑1 (MQ=255)
           ttttttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGGTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATgg                                                                                                                                        <  2:1773844/150‑1 (MQ=255)
             ttttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTg                                                                                                                                      <  2:649955/150‑1 (MQ=255)
              tttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGc                                                                                                                                     <  2:1405914/150‑1 (MQ=255)
                   agaagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCg                                                                                                                                <  1:1454514/150‑1 (MQ=255)
                   agaagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCg                                                                                                                                <  1:2709298/150‑1 (MQ=255)
                           gTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCGTCCGGGAGGAtt                                                                                                                        <  2:1706036/150‑1 (MQ=255)
                           ctGCACCCGGGAGGATGACTCACTGACGTCCGCCCTTCGGGCCGTTGCTGGCGCAACGCTATCATCCCTCGTGCCTGCGGTTAATTCCGCTAACTCTTCGATACCTTCATTGCTTAATTTTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                        <  2:810106/149‑1 (MQ=14)
                               aTCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGaa                                                                                                                    <  2:1631901/150‑1 (MQ=255)
                                    ggaggaTGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                                               <  2:2310468/150‑1 (MQ=255)
                                      aggaTGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                                             <  1:2166818/150‑1 (MQ=255)
                                                 cTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                  >  2:650744/1‑150 (MQ=255)
                                                 cTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                  <  2:1513232/150‑1 (MQ=255)
                                                    aCGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                               <  2:2107700/150‑1 (MQ=255)
                                                       ttCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTGCTGGTGCCTGCGGTTAATTCCGCTATCTCTTCGATACCTTCATTGGTGTAGTTTTCGGAGTATATGGTGCTTCCGGGAGGATTCGAACCTCCGTCCGCTCAGTTCGtttgg                                                                                            >  2:1040362/1‑146 (MQ=14)
                                                       ttCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                            >  2:2077617/1‑150 (MQ=255)
                                                         cGCCCGTCGGGCCGTTGCTGGCGCAACGTTCTCCTTCCTGGTGCCTGCGGTTACTACCGCGATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                          <  2:2172944/150‑1 (MQ=255)
                                                            ccTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                       >  2:1818709/1‑150 (MQ=255)
                                                                   gCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                                  <  1:1456293/148‑1 (MQ=255)
                                                                   gCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                                <  2:1198707/150‑1 (MQ=255)
                                                                   gCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                                >  2:1678010/1‑150 (MQ=255)
                                                                      gTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                             <  2:215838/150‑1 (MQ=255)
                                                                        tGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTTCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                           <  1:1071879/150‑1 (MQ=255)
                                                                        tGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                           <  1:304481/150‑1 (MQ=255)
                                                                          cTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTCCGGAGACTATGGTGCATCCGTGAGGATTCGAACATACGACCGCTCGGTGCGTATCCGTGTACTCTATCCAGCTGa                                                                         >  2:262483/1‑150 (MQ=17)
                                                                          cTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                         >  1:668154/1‑150 (MQ=255)
                                                                          cTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                         >  1:668110/1‑150 (MQ=255)
                                                                          cTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCAGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                         >  2:2238990/1‑150 (MQ=255)
                                                                          cTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGATGAAGGTTACGGAGTATATGGTGCATCAGGGAGGATTCGAACCTACGACCGCTCGGTGCGTAGCCGAGTACTCTATCCAGCTgt                                                                         >  2:262643/1‑149 (MQ=17)
                                                                           tGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                        <  1:1013460/150‑1 (MQ=255)
                                                                           tGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                        <  1:2575507/150‑1 (MQ=255)
                                                                            ggCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATTCCGCTCACTCTTCGATACCTTCAATGCTGAAGCTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTACGTAGCCGAGTACTCTATCCAGCTGAGc                                                                       >  1:1645407/1‑150 (MQ=14)
                                                                             gcgcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                                      >  1:2166668/1‑150 (MQ=255)
                                                                             gcgcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                                      >  1:1001773/1‑150 (MQ=255)
                                                                             gcgcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGCTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                                      >  1:2087392/1‑150 (MQ=255)
                                                                              cgcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                                                     <  2:1950257/150‑1 (MQ=255)
                                                                              cgcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                                                     <  2:1996393/150‑1 (MQ=255)
                                                                              cgcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                                                     <  2:2436461/150‑1 (MQ=255)
                                                                                 aaCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGCGCTACgg                                                                  <  2:1009492/150‑1 (MQ=255)
                                                                                 aaCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                                                  <  2:2555187/150‑1 (MQ=255)
                                                                                 aaCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                                                  <  2:1282473/150‑1 (MQ=255)
                                                                                 aaCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                                                  <  2:1990379/150‑1 (MQ=255)
                                                                                         ccttcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                          <  2:561959/150‑1 (MQ=255)
                                                                                         ccttcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                          <  1:2421733/150‑1 (MQ=255)
                                                                                         ccttcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                          <  1:2307946/150‑1 (MQ=255)
                                                                                         ccttcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCATAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                          <  2:1912059/150‑1 (MQ=255)
                                                                                         ccttcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCATAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                          <  2:1772746/150‑1 (MQ=255)
                                                                                         cctgcctgGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGCGCTACGGATGCATCg                                                          <  2:1652563/150‑1 (MQ=255)
                                                                                              ctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                                                     >  1:1549250/1‑150 (MQ=255)
                                                                                              ctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                                                     >  1:1849958/1‑150 (MQ=255)
                                                                                               tGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaat                                                    >  1:1916638/1‑149 (MQ=40)
                                                                                               tGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaat                                                    >  1:1916647/1‑149 (MQ=40)
                                                                                               tGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaaa                                                    >  1:546660/1‑150 (MQ=255)
                                                                                               tGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaaa                                                    >  2:1421025/1‑150 (MQ=255)
                                                                                               tGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaaa                                                    >  2:770285/1‑150 (MQ=255)
                                                                                                 gTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaattt                                                  >  1:1852773/1‑147 (MQ=37)
                                                                                                 gTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaattt                                                  >  1:2531545/1‑147 (MQ=37)
                                                                                                 gTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACt                                                  >  1:898684/1‑150 (MQ=255)
                                                                                                   gCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaatttat                                                <  1:1678010/150‑6 (MQ=25)
                                                                                                    ccTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACtgag                                               >  2:1979891/1‑147 (MQ=255)
                                                                                                      tGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATCTTAtta                                             >  1:1529941/1‑149 (MQ=255)
                                                                                                         ggTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                                                                    >  2:2516134/1‑124 (MQ=40)
                                                                                                              aTACCGCTATCTCTTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                               <  1:2143087/124‑1 (MQ=17)
                                                                                                              aTACCGCTATCTCTTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                               <  1:1355908/124‑1 (MQ=38)
                                                                                                              aTACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                               >  2:1355908/1‑124 (MQ=40)
                                                                                                              aTACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                               >  2:2143087/1‑124 (MQ=40)
                                                                                                                  cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGaa                                                                                                                    >  1:786902/1‑67 (MQ=25)
                                                                                                                  cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGatat                                 >  1:2432214/1‑150 (MQ=255)
                                                                                                                  cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGatat                                 >  1:457800/1‑150 (MQ=255)
                                                                                                                  cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGATCGTAGCCGATAACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGatat                                 >  1:805235/1‑150 (MQ=255)
                                                                                                                  cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACATCAGACCGCACGGAACGTAGCCGAGAACTATAACCAGCTGTGATACGGATGCCTCGGGAAAACTTATTCTACTGCTGatat                                 >  1:2432210/1‑150 (MQ=14)
                                                                                                                    ctttctcTTCGATTCCTTCATTGATGAATGTTTGGGAGATTATGGTGCATCCGGGAGGTTTCGACCGTGCGACCGCTCGGTTCGTAGCCGTGTACTCTATCCTGCTGAGCTTCGGATGCTTCGGGAAATCTTATTCTTCTTCTGATTTTg                               <  1:32286/147‑1 (MQ=11)
                                                                                                                    cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTg                               <  2:1484798/150‑1 (MQ=255)
                                                                                                                    cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTg                               <  2:473388/150‑1 (MQ=255)
                                                                                                                    cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTAGTCTACTGCTGATATTg                               <  1:29787/150‑1 (MQ=255)
                                                                                                                     tATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGa                              <  2:668154/150‑1 (MQ=255)
                                                                                                                         tctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                          >  1:1960469/1‑86 (MQ=38)
                                                                                                                          ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCg                         >  2:1246011/1‑150 (MQ=255)
                                                                                                                                  ccTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTaaa                 <  1:1129753/150‑1 (MQ=255)
                                                                                                                                    ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGATGATTCGAACCTCCGACCGCTCGGTTCTTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGc               <  1:2348991/150‑1 (MQ=255)
                                                                                                                                    ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGc               <  1:650744/150‑1 (MQ=255)
                                                                                                                                     tCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGcc              >  1:2629789/1‑150 (MQ=255)
                                                                                                                                       aTTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                        >  1:1731135/1‑42 (MQ=255)
                                                                                                                                            tGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCaa       >  2:2337471/1‑150 (MQ=255)
                                                                                                                                            tGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCaa       >  2:877224/1‑150 (MQ=255)
                                                                                                                                                 gTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAag  <  1:1653612/150‑1 (MQ=255)
                                                                                                                                                 gTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAag  <  1:902905/150‑1 (MQ=255)
                                                                                                                                                 gTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAag  <  1:2727982/150‑1 (MQ=255)
                                                                                                                                                 gTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAag  <  1:1093792/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAG  >  NC_000913/2817626‑2817920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: