Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,581 | A→C | 19.5% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,581 | 0 | A | C | 19.5% | 208.5 / 25.7 | 128 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base A (37/64); new base C (4/23); total (41/87) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.68e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 135 total aligned reads displayed.
CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGC > NC_000913/164432‑164729 | ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAATAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa > 2:3208393/1‑150 (MQ=255) ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa > 2:2148546/1‑150 (MQ=255) ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa > 2:5240606/1‑150 (MQ=255) ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGAACCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGCTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCAGGGGTGCGGGCa > 2:5762826/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 1:5795045/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:4069562/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:4069496/1‑150 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa > 2:1939067/1‑150 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgc > 1:4583478/1‑150 (MQ=255) aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc > 1:5566750/1‑150 (MQ=255) agagATGAAAGGGCGATATACCAAACATGTCTGGCCGGACGACCCGTCAAAGACTGCACCGACGCGACGGACGTAAAAGTATACGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGCGAGGGCCGGTGTGAGTGCAGCAGCGCGCACGGtc > 1:2404596/1‑150 (MQ=37) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccc > 2:747474/1‑150 (MQ=255) tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTggaccca > 1:3819868/1‑149 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctc > 1:4191711/1‑150 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccgc > 2:4845379/1‑148 (MQ=255) aacgggCGTTATCCCAAACAGGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAATGTATTCTTATGGTTGTTTTTCGCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca < 2:3312883/147‑1 (MQ=38) aaaGGGCTTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca < 2:3312890/150‑1 (MQ=255) aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacccacccc > 1:4362331/1‑145 (MQ=255) ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcaccccaaacca > 2:2782739/1‑143 (MQ=255) ggCGTTAGCCCCACCCGGCCTGGCCGGCCGCCCCGGCAAATCCTGCCCCGACGCGACGGACTAAAAAGTATTCGTAGGGTGGGTTTTCGCCCTCTCCCGGTGGGCGTGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTcaccctcaccct < 2:4656628/150‑1 (MQ=255) ttATCCCAACCTTGTGGGGCCGGACGACCGGGAAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTACGGTTGGTTTTCTCCCTGGCCGTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:4218656/150‑1 (MQ=255) ttATCCCAACCATGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGTTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:3689041/150‑1 (MQ=255) tCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACACCCCAAccccc > 1:1209411/1‑148 (MQ=38) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCACCCGCACCCCAAcccgca > 2:3862555/1‑147 (MQ=38) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGTCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCCCACCCCAAaccacaa > 1:4089375/1‑146 (MQ=38) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCCCACCCCAAaccacac > 1:5790161/1‑146 (MQ=38) caacccggTCGGGCCGGACGACCCGGCAAATCCTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCGCCCGGGGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:5289973/143‑1 (MQ=255) cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCCCACCCACAcccaccac > 2:3940269/1‑145 (MQ=38) cAAACATGGCTGGCCGGAGGACCGGGAAAATACGGCACCGAGGCGAGGGAGTAAAATGTTTGCGTTTGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:4191711/150‑1 (MQ=25) aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGGCCCCCACCCAAAccccacacc > 1:4131386/1‑144 (MQ=37) cATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCGCAACCAAAACCACTCCcaca > 1:1939662/1‑150 (MQ=37) tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCGCACCCGAACCCACTCCCACCAgggtg > 2:2420776/1‑148 (MQ=38) gCCGGACGACCCGCAAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:396764/150‑1 (MQ=255) gCCGGACCCCCCGGCAAATACTGCACCGACGCGAGGCACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCCCCGTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:5085468/150‑1 (MQ=38) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCACCCCCCAAACCCCGCCCCCAAGGGCGAggg > 1:862568/1‑150 (MQ=37) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 2:3529648/150‑1 (MQ=255) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCTCGCACGTTGGCCCGCACCCAAAACCACGCCCACCAGGGAggggggg > 1:146409/1‑149 (MQ=37) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGGGCGCCCACCCCCAAACCCACACCCACCACGGCGGGGGGGCCgcgc > 1:4198110/1‑147 (MQ=21) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa < 2:3573016/95‑1 (MQ=255) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa > 1:3573016/1‑95 (MQ=255) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCAAAACCACACCCACAAGGGGGGGGGGGcccaag > 1:887148/1‑146 (MQ=25) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGGCCCACACCCAAAACCAAACCCACAAggggggggggggcgggc > 1:518888/1‑143 (MQ=21) gtaaaaTACTGCACCTACGCGTCGGACTAAAATCTATTCTTTAGTTTTTTTTTCTCCATATCCCTTTGGTTGTGGGCGGGGGTTTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa < 2:2404596/147‑1 (MQ=255) ggaaaaTCCTGCCCCGCCGCGACGGCCGAAAATGTAGTGGTACGTTTGGTTTGCCCCCCCCCCGGGGGGTTGTGGGCCGGGGTGGGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa < 2:2072678/147‑1 (MQ=255) aaaaaACTGCACCGCCGCGACGCACTAAAACCTAGTCTAATGGTTGGTTTCCACCCTCCCCGGGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGc < 1:2875702/149‑1 (MQ=255) gCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAttt < 2:3433062/150‑1 (MQ=255) cACCGCCGGGCCGTACGAAATTGTTTTCTTATGTTTGGTTTCCCCCCTCTCCGGGGGGGTGCGGGCGGGGTGGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:4460303/150‑1 (MQ=255) cACCGACGCGACGGACGAAAAATTATTCTTATGGTTGTTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:1916106/150‑1 (MQ=255) cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:1916362/150‑1 (MQ=255) aCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCGCCCACACCCAAAACCACACCCACAAGGGCGCGGGGGCCGCACGAGCACAAAATTTg > 1:2379124/1‑150 (MQ=25) aCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACACACACCCAAAAACAAAACCAAAAGGGAGAGTGGGCCCAACGAACACAAAatttt > 2:553910/1‑149 (MQ=18) ccgccgCGCCGGACGAAAAAGTATTCGTATGGTTGGTTTTCGCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2963718/150‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCTAAACCACACCCACAAGGGGGAGGGGCCCGATAGCGCACaaaaattgt > 2:2862913/1‑144 (MQ=25) cgcggtacgAAAACTTATGCTTAGGGTTGTTGTCCTCCCACGCCGGGTGGGTGTGGGCGGGGGTGCGGGAATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTCGAGAGata < 2:3914976/144‑1 (MQ=255) acgtactAAAAAGTATTCTTACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 1:1334586/150‑1 (MQ=38) acggactAAAATGTATTTGTATGGTTGGTTTTCCCCCTCCCCCTGTGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 1:114993/150‑1 (MQ=255) aaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt < 2:4381737/150‑1 (MQ=255) aaGTTTTCTTACGGTTGGTTTTCTCCCTCGCCCTGTGGGCGCGGGCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:1810135/150‑1 (MQ=255) gTATTCGTAAGGTTGGTTTTCGCCCTCGCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:1385112/150‑1 (MQ=255) ttttCGTTGGGTGGGTTTCCCCCCCCGCCCTGTGGGCGAGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:2070262/148‑1 (MQ=255) aTTCCTAAGTTGGGTTTTCGCCCTCTCCGTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 2:5584959/150‑1 (MQ=38) cgcacggttgtttTACACCCCCCCCCTGGGGGAGGGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAAc < 1:495068/145‑1 (MQ=255) tatggttggttTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag < 1:3508384/147‑1 (MQ=255) tAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCCCACCCCAACCCGCTCCCGAAACGGCGAGGGGCCCCAACGCGCACAAAAGGTTGGAGTTATATTCTTCTGTCTTGTAACag > 2:3907231/1‑150 (MQ=16) cggttggttTTCTCCCACTCCCTGTGGTTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:4556712/149‑1 (MQ=255) aGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:447783/150‑1 (MQ=255) gtttgtttTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag < 1:1929166/148‑1 (MQ=255) ggttggttTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag < 2:2872899/150‑1 (MQ=255) ccctctccctGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGTGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:4845379/150‑1 (MQ=255) ccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 2:3218282/150‑1 (MQ=255) cctctccgtGTGGGCGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:31702/150‑1 (MQ=255) cccggggggAGTGGGCCGGGGTGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt < 2:236705/144‑1 (MQ=255) cctGGGGGTGGGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt < 1:747474/150‑1 (MQ=255) gtgtgGGAGTGGCCCGGGGTGAGGGAATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg < 2:5498591/149‑1 (MQ=255) ctGTGGTAGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg < 2:1939662/150‑1 (MQ=255) ggggAGTGGGCCGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:4408209/149‑1 (MQ=255) ggtagcgggCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGcc < 1:1799261/144‑1 (MQ=255) ggAGAGGGCCGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTGGAGAGATATCCTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 2:1255586/150‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCGCACCCAAACCCGAACCCGCCACGGAGGGGGGACCGATCGAGCACAAATTTTTTGAGATATCATCTTCTGTCGTGTACAAGAAGAACAGCAAAACCGGCTGTTGCGccag > 2:5310312/1‑148 (MQ=255) agagGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGa < 1:1864830/150‑1 (MQ=255) gggCGGGGGTGCGGCCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata < 1:1013270/150‑1 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACACCCCCCAGGGGGAGGGGGCCGATAGAGCCCAAAATTTGTGAGATAAATTATTATGTATTTTAACAGAACCACAGAAAAAAGGGCTTTTTGGCCCGAaaat > 2:3055707/1‑150 (MQ=255) ccGGGGTGAGGGCATCAGCGCGCACGTTGACCCACAACCAAACCCACAACCACAACGGAGAGGGGACCCATCGAGCACAAAATTTGTTAGTTATATTATTAATTATTGTAAAAGAAGAAAAAAAAAAAGGGCTTTTTAGCCGGAATAATg > 2:4775643/1‑150 (MQ=255) ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCACCCAAAACCACTCCCACAACGGGGGGGGGGCCCCTAGCGCCCAAAATTTTTGATATATAATATTATTTGTTTTTAAAGAAGAAAACAAAAAACGGCGTTTTAGCCGGAATATTg > 2:594105/1‑150 (MQ=255) gggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:185420/149‑1 (MQ=255) cGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:1778392/150‑1 (MQ=255) cGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 2:3159654/150‑1 (MQ=255) ggggggAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:2646136/150‑1 (MQ=255) ggggTGTGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:4535029/150‑1 (MQ=255) ggggTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:5318152/150‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCACGGGAGAGGGGACCGATCGAGCACAAATGGTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:4534936/150‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCACGTGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1786061/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:3479189/150‑1 (MQ=255) gggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTTTAACAGAAGAACAGAAAATCGGTCTTTTGCGCCTGAATATTGCgg < 2:3641622/150‑1 (MQ=255) gggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg < 2:1560555/150‑1 (MQ=255) ggggAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:1609059/147‑1 (MQ=255) ggTGAGGGCCTCAGCGCGCCCGTTCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:890129/150‑1 (MQ=255) gggCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 2:5270309/150‑1 (MQ=255) ggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:3719479/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:5795045/150‑1 (MQ=255) | CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGC > NC_000913/164432‑164729 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |