Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 882,356 A→T 6.1% F127I (TTT→ATT)  deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913882,3560AT 6.1% 769.2 / 3.2 252F127I (TTT→ATT) deoRdeoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
Reads supporting (aligned to +/- strand):  ref base A (121/104);  new base T (3/21);  total (124/128)
Fisher's exact test for biased strand distribution p-value = 1.40e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

CACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCT  >  NC_000913/882207‑882504
                                                                                                                                                     |                                                                                                                                                    
 aCGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAg                                                                                                                                                     <  2:4511328/150‑1 (MQ=255)
    cccgccGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTa                                                                                                                                                  <  1:3076611/150‑1 (MQ=255)
    cccgccGCAGAATAAAAAGCGATAACCGGGCAAAAAATATACAGCGTTTGCTGAAAATCGATGCGATTGAAAATGGCGTTGCTCGCGTGAAATTCACCACCCCAAACAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTa                                                                                                                                                  <  2:603822/150‑1 (MQ=255)
       gccgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAAGGGGGTGTCTCTTTCAGCGCCCGAAAGGtctgt                                                                                                                                               >  1:2625947/1‑146 (MQ=255)
        ccgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTa                                                                                                                                              >  2:5622397/1‑150 (MQ=255)
         cgcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAg                                                                                                                                             >  2:2121126/1‑150 (MQ=255)
          gcAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGc                                                                                                                                            <  1:157313/150‑1 (MQ=255)
           cAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCg                                                                                                                                           <  1:3805823/150‑1 (MQ=255)
             gAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGaa                                                                                                                                         <  2:2520817/150‑1 (MQ=255)
             gAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGaa                                                                                                                                         <  2:5029838/150‑1 (MQ=255)
                tAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATaa                                                                                                                                      <  1:1424259/150‑1 (MQ=255)
                tAAAAAGCGATATCCGGGCAAAAATTATGCAGCGTGTGCTGAAAATCGATGGGTTTGAACATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATaa                                                                                                                                      <  2:5640648/150‑1 (MQ=255)
                  aaaaGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACa                                                                                                                                    >  2:3684022/1‑150 (MQ=255)
                   aaaGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATTGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCTAATAACaa                                                                                                                                   >  2:5673136/1‑150 (MQ=255)
                   aaaGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACaa                                                                                                                                   >  2:1439045/1‑150 (MQ=255)
                   aaaGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTGTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACaa                                                                                                                                   >  1:1759774/1‑150 (MQ=255)
                    aaGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACaaa                                                                                                                                  <  2:2495269/150‑1 (MQ=255)
                    aaGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACaaa                                                                                                                                  <  2:1811921/150‑1 (MQ=255)
                       cGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAcgg                                                                                                                               <  2:2858123/150‑1 (MQ=255)
                       cGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAcgg                                                                                                                               <  2:3028570/150‑1 (MQ=255)
                       cGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAAcgg                                                                                                                               <  2:446592/150‑1 (MQ=255)
                            tCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGACACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAAAAAAcggatgta                                                                                                                          >  2:3762915/1‑145 (MQ=255)
                               gggCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTaaaa                                                                                                                       >  1:1683115/1‑150 (MQ=255)
                                  cAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAggg                                                                                                                    >  2:255344/1‑150 (MQ=255)
                                     aaaTTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAACGGGAtt                                                                                                                 >  2:3498722/1‑150 (MQ=255)
                                        ttattCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCGAAGAAACGCGCGGCAATGGGGTTTCTATTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCa                                                                                                              <  2:2670195/150‑1 (MQ=255)
                                         tattCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCAt                                                                                                             <  2:298666/150‑1 (MQ=255)
                                          attCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCAtt                                                                                                            <  2:396428/150‑1 (MQ=255)
                                          atcccgCGTTTGCTTAAAATCGTTGGTTTTTAAATTGGCGTGGCGGGCGTTAAATACACCCCCCAAAACAACCGCGCGGAATTGGGGTTACTCTTTCAGCGCCTGAATGTTATTTAGCTAAAAACAAACGGCGGTAAAAGGGATTTCAtt                                                                                                            <  1:116428/145‑1 (MQ=255)
                                           ttCAGCGTTTGCTGAAAATCGTTGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTa                                                                                                           <  2:3717065/150‑1 (MQ=255)
                                            tCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTAt                                                                                                          >  1:2019370/1‑150 (MQ=255)
                                            tCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTAt                                                                                                          >  2:2873036/1‑150 (MQ=255)
                                             ccgcttttGCTGAAAATCGTGGGTTTTTAAATTGGCGTTGGTGGCGTTAACTTCACCACCGCAAAGAACCGCGCGGCATTGGGGTTTCTCTTTCAGCGCCATAATGTTTTTTAGCTAATAACACACGGCGGTAAAAGGGATTTCATTATc                                                                                                         <  1:5264257/145‑1 (MQ=255)
                                              aGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCa                                                                                                        >  2:1677625/1‑150 (MQ=255)
                                              aGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCa                                                                                                        >  2:1288427/1‑150 (MQ=255)
                                                 gTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATc                                                                                                     >  2:3243652/1‑150 (MQ=255)
                                                 gTTGGCTGAAATTGGTGGGGTTTTAAATTGGCGTTGCGGGGGTGAAATTCACCACCGCAAAGACCGGCGGGGCATGGGGGTTTCTCTTTCCGCGCCAGAATGGTTTTTCGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATc                                                                                                     <  1:5240329/150‑1 (MQ=255)
                                                  tttGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCg                                                                                                    <  2:1000980/150‑1 (MQ=255)
                                                     gCTTAAAATCGTTGGTTTTTAAATTGGGGTTGGGGGGGTCAAATACCCCACCCAAAAGAACCGCGGGGCATTGGGTTTTCTCTTTCTGCGCCATAACGGTTTTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCtt                                                                                                 <  1:4873109/150‑1 (MQ=255)
                                                     gCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCtt                                                                                                 >  2:3066762/1‑150 (MQ=255)
                                                       tGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCa                                                                                               >  1:2866337/1‑150 (MQ=255)
                                                        gAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCaa                                                                                              >  1:3996034/1‑150 (MQ=255)
                                                             tCGATGGTTTTGAATTGGGGGTGGCTGGCTTTAATTTCACCCCCGCAAAGAAAGGCGCGGCATTGGGTTTTCTCTTTCAGCGCCATAATGTTATTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATc                                                                                         <  2:4394645/150‑1 (MQ=255)
                                                             tCGATGGTTTTGAAAATGGCGTTGCTGGCGTTAAATTCACCACCGCAAAGAAACGCGCGGCATTGGGTTTTCTCTTTCAGCGCCATAATTGTTTTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATc                                                                                         <  1:4188878/150‑1 (MQ=255)
                                                              cGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATcc                                                                                        <  2:773237/150‑1 (MQ=255)
                                                                  gggTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACgg                                                                                    <  2:4156132/150‑1 (MQ=255)
                                                                   ggTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGc                                                                                   <  2:1840608/150‑1 (MQ=255)
                                                                     tttGAAATTGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAATGGTATTTAGCGAATAACAAACGGCGGTAAATGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgt                                                                                 >  2:1175371/1‑150 (MQ=255)
                                                                     tttGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgt                                                                                 >  2:4693505/1‑150 (MQ=255)
                                                                     tttGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgt                                                                                 >  2:2222972/1‑150 (MQ=255)
                                                                      ttGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtg                                                                                >  2:5572663/1‑150 (MQ=255)
                                                                           aaTGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCgtggtggt                                                                           <  2:721934/150‑1 (MQ=255)
                                                                             tGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGc                                                                         <  2:4068211/150‑1 (MQ=255)
                                                                               ggtttGCTGGGTTTAATTTAACCACCGAAAAGAACCGGGCGGAATGGGGGTTTCTCTTACAGCGCAATAATGTTTTTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCca                                                                       <  1:4519984/147‑1 (MQ=255)
                                                                               gggTTGCTGGCTTTAAATTCACCACCGCAAATAAACGGGGGGCTCTGGGTTTTCTCTTTCAGCGCCATAATGTTATTTAGCTAATAAAAAACGGCGGTAAAAGGTATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCca                                                                       <  1:500563/148‑1 (MQ=255)
                                                                               gCGTTGCTGGCGTTAAATTCACCACCCAAAAGAAACGCGCGGCAATGGGGTTTATCTTTCAGCGCATTAATGTTTTTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCca                                                                       <  1:921668/150‑1 (MQ=255)
                                                                               gCGGTGCTGGCGTGAAATTCACCACCGCAAAGAACCGCGCGGCAATGGGTTTTCTCTTTCAGCGCCAGAACGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCca                                                                       <  1:4681147/150‑1 (MQ=255)
                                                                                   tGCTGGCGTTAAATTCACCACCGCAAAGAAACGCGCGGCTTTGGGGTTCCTCTTTCAGGTCCAGAATGGTTTTTAGCTATTAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGt                                                                   <  1:1109206/150‑1 (MQ=255)
                                                                                    gCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTc                                                                  <  2:3217723/150‑1 (MQ=255)
                                                                                     cTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCa                                                                 >  1:3741587/1‑150 (MQ=255)
                                                                                      tGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCaa                                                                >  2:5092306/1‑150 (MQ=255)
                                                                                        gCGTTAAATTCACCACCGCAAAGAAACGCGCGGCATTGGGGTTTCTCTTTCAGCGCCAGAATGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCaaaa                                                              <  1:3298581/150‑1 (MQ=255)
                                                                                        gCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCaaaa                                                              >  1:5339174/1‑150 (MQ=255)
                                                                                             aaaTTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAagag                                                         <  1:621745/150‑1 (MQ=255)
                                                                                              aagtCACCACCGAAAAGAAACGCGCGGCATTGGGGTTTCTATTTCCGCGACATAATTTTATTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAgg                                                        <  1:2472213/147‑1 (MQ=255)
                                                                                              aaTTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAgg                                                        <  2:2304181/150‑1 (MQ=255)
                                                                                              aaTTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAgg                                                        <  2:2304093/150‑1 (MQ=255)
                                                                                              aaTTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAgg                                                        <  1:3302713/150‑1 (MQ=255)
                                                                                                ttCACCGCCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGACGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCACAAAAGAGGGt                                                      >  2:5535878/1‑150 (MQ=255)
                                                                                                      cccGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATc                                                <  1:5158349/149‑1 (MQ=255)
                                                                                                      accGCAAAGAAACGCGCGGCATTGGGTTTTCTCTTTCAGCGCCAGAATGTTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATc                                                <  1:2266596/150‑1 (MQ=255)
                                                                                                      accGAAAACAAACGCGCGTCAATGTGTTTTATCTTAAAGCGCCAGAAAGTTATTTAGCTAAAAACAAACGGAGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGTTGGTGCCACAGTCAAAAAAGAGGGTCTGATc                                                <  1:3726475/150‑1 (MQ=255)
                                                                                                       ccGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCg                                               >  2:627318/1‑150 (MQ=255)
                                                                                                        cgaaaaGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCTAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCgg                                              <  1:4365330/147‑1 (MQ=255)
                                                                                                         gCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCggg                                             >  2:846397/1‑150 (MQ=255)
                                                                                                          cAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGt                                            >  1:4041049/1‑150 (MQ=255)
                                                                                                           aaaGAAACGCGCGGCACTGGGGTTTCTCTTTCAGCGCCAGAAAGTTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGtt                                           <  1:278486/150‑1 (MQ=255)
                                                                                                           aaaGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGtt                                           >  1:423805/1‑150 (MQ=255)
                                                                                                            aaGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTc                                          >  1:1830240/1‑150 (MQ=255)
                                                                                                             aGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCt                                         <  2:3888821/150‑1 (MQ=255)
                                                                                                               aaaCGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTAc                                       >  2:4857945/1‑150 (MQ=255)
                                                                                                               aaaCGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTAc                                       >  2:2087828/1‑150 (MQ=255)
                                                                                                                aaCGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTCCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTAcc                                      <  2:3834062/150‑1 (MQ=255)
                                                                                                                         cattGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCa                             <  1:871956/147‑1 (MQ=255)
                                                                                                                           aTGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGc                           >  2:3137380/1‑150 (MQ=255)
                                                                                                                                 tttCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGttt                     <  1:5616952/150‑1 (MQ=255)
                                                                                                                                 tttCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGttt                     >  2:4144510/1‑150 (MQ=255)
                                                                                                                                       tttCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTgccgc               >  1:5329949/1‑150 (MQ=255)
                                                                                                                                        ttCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTgccgcc              >  2:5657047/1‑150 (MQ=255)
                                                                                                                                         tCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGccc             <  1:416183/150‑1 (MQ=255)
                                                                                                                                          cAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCg            <  2:3996034/150‑1 (MQ=255)
                                                                                                                                           aGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCgg           >  2:5807578/1‑150 (MQ=255)
                                                                                                                                             cgcCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCg         >  2:3656025/1‑150 (MQ=255)
                                                                                                                                              gcCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGt        >  1:126729/1‑150 (MQ=255)
                                                                                                                                                cAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGttt      >  2:5190588/1‑150 (MQ=255)
                                                                                                                                                 aGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGtttt     >  2:934443/1‑150 (MQ=255)
                                                                                                                                                   aaaGGTATTTATAGAATAACAAACGGCGGTAAAAGGTATTTCATTATCAATCTCTTCAATAATCCACGGCGTGGTGTTGCCACAGTCAATAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCATCCAGTTTTGCCGACCGGCGTTTttc   <  2:5395511/150‑1 (MQ=255)
                                                                                                                                                   aaaGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTttc   <  1:1109421/150‑1 (MQ=255)
                                                                                                                                                    aaGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTttct  <  2:5339174/150‑1 (MQ=255)
                                                                                                                                                    aaGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTttct  <  1:5486485/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CACGCCCGCCGCAGAATAAAAAGCGATATCCGGGCAAAAATTATTCAGCGTTTGCTGAAAATCGATGGGTTTGAAAATGGCGTTGCTGGCGTGAAATTCACCACCGCAAAGAAACGCGCGGCAATGGGGTTTCTCTTTCAGCGCCAGAAAGGTATTTAGCGAATAACAAACGGCGGTAAAAGGGATTTCATTATCAATCGCTTCAATAATCCACGGCGTGGTGGTGCCACAGTCAAAAAAGAGGGTCTGATCGGGTTCTACCAGCGTCGCAGCCAGTTTTGCCGCCCGGCGTTTTTCT  >  NC_000913/882207‑882504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑