Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,228 C→A 11.7% intergenic (‑24/+284) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2280CA11.7% 546.0 / 8.7 241intergenic (‑24/+284)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (88/107);  new base A (32/3);  total (131/110)
Fisher's exact test for biased strand distribution p-value = 1.77e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGG  >  NC_000913/2177079‑2177377
                                                                                                                                                      |                                                                                                                                                     
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                       <  2:2868666/150‑1 (MQ=255)
  gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGa                                                                                                                                                     >  1:321816/1‑150 (MQ=255)
  gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGa                                                                                                                                                     <  1:1641948/150‑1 (MQ=255)
   cAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGag                                                                                                                                                    >  2:651024/1‑149 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGAtt                                                                                                                                                   >  1:2222294/1‑150 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAtt                                                                                                                                                   >  2:5276020/1‑150 (MQ=255)
     ggTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAttt                                                                                                                                                  <  2:4141751/150‑1 (MQ=255)
     ggTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAttt                                                                                                                                                  <  2:2097754/150‑1 (MQ=255)
       ttGGCAGCTGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                <  2:2669305/150‑1 (MQ=255)
          gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                             <  1:1624946/150‑1 (MQ=255)
           cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                            >  2:4758287/1‑150 (MQ=255)
           cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAat                                                                                                                                            >  2:3052309/1‑150 (MQ=255)
            aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                           >  2:1335153/1‑150 (MQ=255)
              cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                         >  2:4245870/1‑150 (MQ=255)
              cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGTTTTAAAAataaa                                                                                                                                         >  1:2540009/1‑148 (MQ=255)
                gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAt                                                                                                                                       >  2:74851/1‑150 (MQ=255)
                    cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                   >  1:5739841/1‑150 (MQ=255)
                     tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAat                                                                                                                                  >  1:1467890/1‑150 (MQ=255)
                      accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATGAAatt                                                                                                                                 >  2:88763/1‑150 (MQ=255)
                       ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAAAGAAaata                                                                                                                                >  2:2962036/1‑150 (MQ=255)
                       ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                <  1:977610/150‑1 (MQ=255)
                       ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAaata                                                                                                                                >  2:4012664/1‑150 (MQ=255)
                       ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAaata                                                                                                                                >  1:4142813/1‑150 (MQ=255)
                       ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAatta                                                                                                                                >  1:302916/1‑150 (MQ=255)
                           cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                            >  2:2318026/1‑150 (MQ=255)
                             cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAtttttt                                                                                                                          >  2:2740005/1‑150 (MQ=255)
                             cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAATTAATtttt                                                                                                                          >  2:2739884/1‑150 (MQ=255)
                               tGCATCGTTTCGAGATTGTGAATATTGACTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTAGATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                        <  2:5481352/150‑1 (MQ=255)
                                gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATTAAAATATtttttttt                                                                                                                       >  2:2636311/1‑150 (MQ=255)
                                gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATTAAATTATTTGttttt                                                                                                                       >  1:1207814/1‑150 (MQ=255)
                                 cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAATAATTAAATTATTTTTTTTTa                                                                                                                      >  2:2702679/1‑150 (MQ=255)
                                   tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATGAAATTATTTTTTTTTaaa                                                                                                                    >  1:2552688/1‑150 (MQ=255)
                                    cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAat                                                                                                                   <  2:1324379/150‑1 (MQ=255)
                                      tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAatat                                                                                                                 >  2:5588836/1‑150 (MQ=255)
                                      tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                 >  2:4992767/1‑150 (MQ=255)
                                        tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATct                                                                                                               >  2:5157902/1‑149 (MQ=255)
                                        tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                               <  2:2012054/150‑1 (MQ=255)
                                          gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                             >  2:5786912/1‑150 (MQ=255)
                                           agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAAATATTTTTTTTTAAATATCgata                                                                                                            >  2:2709512/1‑148 (MQ=255)
                                            gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                           >  2:5680157/1‑150 (MQ=255)
                                               tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                        <  1:291935/150‑1 (MQ=255)
                                               tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                        <  1:2397726/150‑1 (MQ=255)
                                                 tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAAGAAATTAATTGTTTTTAAAAATCGAGATAACGa                                                                                                      >  1:4727812/1‑150 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                     >  2:4537470/1‑150 (MQ=255)
                                                     tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                  <  1:2703828/150‑1 (MQ=255)
                                                     tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTACGAATTAAAATAAAAAAAAAATATTTTTTTTTAAATATAAACATAAcaaaaac                                                                                                  >  1:1017568/1‑144 (MQ=255)
                                                      atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                 <  2:355056/150‑1 (MQ=255)
                                                      atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                 <  1:1096340/150‑1 (MQ=255)
                                                       ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGCTAAAGATCACaa                                                                                                >  2:3643216/1‑150 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAAATATTTGTTTTTAAATATAGAGATAAAGATAACaaaaa                                                                                             >  1:5587040/1‑150 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                             <  2:1219210/150‑1 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                             <  2:1167111/150‑1 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                             <  2:5487115/150‑1 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAAAATCGAGATAACGATCACaaaaa                                                                                             >  1:3182784/1‑150 (MQ=255)
                                                             gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATGAAAATTTTTGTTTTTAAATATAGCGATAACGCTAACCAAAACGa                                                                                          >  1:2729447/1‑150 (MQ=255)
                                                               cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCCCAAAAACGACa                                                                                        >  2:5820606/1‑150 (MQ=255)
                                                               cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAATAAAATATTTTTTTTTTAATATCGAGCTAAAGATAACAAAAAAGACa                                                                                        >  1:523815/1‑150 (MQ=255)
                                                                 gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTCAAAATATAAATAAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGACaaa                                                                                      >  1:742887/1‑149 (MQ=255)
                                                                      gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAATATAATTTAATTATTTTTTTTTTATTATATAGTTTAAGATGACAAAAAATACAaaattta                                                                                 >  1:1933797/1‑147 (MQ=255)
                                                                      gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATTTAATTATTTTTTTTTTAATTTAGAGATAAAGATAACAAAAAAGACAATATTaa                                                                                 >  1:1933700/1‑150 (MQ=255)
                                                                       cATAACCGCCGCGCTGTGCGGTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGCTTTCAAAAAAAAATGAAATTATTTTTTTTTAAATATCGAGATAACCATCACAAAAACGACAAAATGaca                                                                                >  2:1663581/1‑148 (MQ=255)
                                                                        aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATCGAGATAAAGATCACAAAAACGACAATATGaaaa                                                                               >  2:17430/1‑150 (MQ=255)
                                                                             cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                          >  2:833287/1‑150 (MQ=255)
                                                                               ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTAGATTTAAAAATAAAAAGAAAATTTTTGTTTTTTTATATAGAGATAAAGATCACAAAAAAGACAAAATGAAAATTATTCg                                                                        >  1:1432425/1‑150 (MQ=255)
                                                                                         cGTTTTTCAGAATCTGCTTTGTCGATACCACGTACATTTTCATAGCCTGTCTTTTTTTTTCGTTTTCAAAATATAATGAAATTATTTTTTTTTAATTATCGATATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  1:3075272/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAATAAAAAATTTTTTTTTTAATATGGATCTAACCATCACAAAAAAAAAAAAATTAAAAATATTAGAGGATTGaaa                                                              >  1:409912/1‑150 (MQ=255)
                                                                                              ttCAGCATCTGCTTTGTCTATACCACGTACATTTTCATAGCCTGTCGTTTGTTTTCTATTTAAAAATATAATTAATTTATTTTTTTTTAAATATCGATATAACGAAAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                         <  1:306184/150‑1 (MQ=255)
                                                                                              ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACGATAAAAAAAAAGACAATATGAAAATTATTAGAGGGGTGAAAGGCaa                                                         >  2:71596/1‑150 (MQ=255)
                                                                                               tCAGAATCTGCTTTGTCTAAACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTAAAAAATATAATTAATTTATTTTTTTTTAAATATCGATATACCCAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                        <  1:44577/150‑1 (MQ=255)
                                                                                                     tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAAAGAAAATATTTTTTTTTTAATATAGAGAGAACGATAAAAAAAAACACAAAATAAAAAATAATAGATGAGGGTAAGGCaaaaaaaac                                                  >  1:3491463/1‑148 (MQ=255)
                                                                                                      cTGCTTTGTCGATACCACGTACATTTTCATATCCTTTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                 <  2:249083/150‑1 (MQ=255)
                                                                                                         cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                              <  2:4673696/150‑1 (MQ=255)
                                                                                                           ttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGATGAGTGAAAGGCAAAAAAACGGCCTcc                                            >  2:5175679/1‑150 (MQ=255)
                                                                                                             gTCGATACCACGAAATTTTTCATTTCCTGTCTTTTTTTTTCTTTTAAAAAATATATTTAAATTATTTTTTTTTAAATTTGTTTATAACGATAAAAAAAACGACAATTTTAAATTTTTTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCg                                          <  1:3890928/150‑1 (MQ=255)
                                                                                                              tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                         >  2:3294558/1‑150 (MQ=255)
                                                                                                                taTACCACGAAAATTTTAATATCCTTTCGTTTTTTTTATTTTTAAAAATTTAATTAATTTTTTTTTTTTTAAATATCGAGAAAACGATAAAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                       <  1:4793821/149‑1 (MQ=255)
                                                                                                                gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                       >  2:5707182/1‑150 (MQ=255)
                                                                                                                      aCGTAATTTTTCATATCCTTTTTTTTGTTTTCTATTAAAAAATATAATGAAATTTTTTTTTTTTAAATTTCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                 <  1:4955241/150‑1 (MQ=255)
                                                                                                                       cttaaaTTTTAATAGCCTTTCGTTTTTTTACTATTAAAAAATATAATCAAATTTTTTTTTTTTAAATATCTATATAACTAAAACAAAAACGAAAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                <  1:5428462/145‑1 (MQ=255)
                                                                                                                          aaaTTTTCATCACCTGTCTTTTTTTTTCGATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                             <  1:4758287/148‑1 (MQ=255)
                                                                                                                           cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAGGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTa                            <  2:1713636/150‑1 (MQ=255)
                                                                                                                                tcttatCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                       <  1:1697249/147‑1 (MQ=255)
                                                                                                                                tCATATCCTGTGGTTTGTTTTCTATTTCAAAATTTCATGACATTATTTGTTTTTAAATATCGAGATAACGTTCACCAAAACGACACTATGAAAATTATTCGAGGAGTGAAAGGCAATAATACTGGCTCCCGATAGGGAAGCCGTAGCaaa                       <  2:5268851/150‑1 (MQ=255)
                                                                                                                                tCATATCCTGTCTTTTGTTTACTATTAAAAAATATAATTAATTTTTTTTTTTTAAAATATCGATAAACCTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                       <  1:1975215/150‑1 (MQ=255)
                                                                                                                                tCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAATTTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                       <  1:5721345/150‑1 (MQ=255)
                                                                                                                                    atCCTGTCGTTTGTTTTCTATTTCAAAATATAATAAAATTATTTGTTTTTAAATATCTATATAACGAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                   <  1:4567575/150‑1 (MQ=255)
                                                                                                                                      ccTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                 <  1:3796180/150‑1 (MQ=255)
                                                                                                                                        tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt               <  1:2144720/150‑1 (MQ=255)
                                                                                                                                          tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAATGTGCGCgtgt             >  2:3218499/1‑150 (MQ=255)
                                                                                                                                          tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt             >  2:44427/1‑150 (MQ=255)
                                                                                                                                           cGTTTGTTTTCTATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt            <  1:3643216/150‑1 (MQ=255)
                                                                                                                                            gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt           >  2:2242794/1‑150 (MQ=255)
                                                                                                                                             tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt          >  2:4314412/1‑150 (MQ=255)
                                                                                                                                               ttttttCGATTTAAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa        <  1:5118736/148‑1 (MQ=255)
                                                                                                                                               tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa        <  2:5624820/150‑1 (MQ=255)
                                                                                                                                                 ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAATAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg      >  2:4651959/1‑150 (MQ=255)
                                                                                                                                                  tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAATTTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc     >  1:2815760/1‑150 (MQ=255)
                                                                                                                                                  tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc     >  2:3682891/1‑150 (MQ=255)
                                                                                                                                                   ttCGATTTCAAAATATAATGAAATTTTTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAATGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc    <  2:3113278/150‑1 (MQ=255)
                                                                                                                                                      cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg  >  2:4885460/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGG  >  NC_000913/2177079‑2177377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: