Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 18.2% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT18.2% 541.5 / 26.4 257intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (94/102);  new base T (32/21);  total (127/130)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

TCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177098‑2177394
                                                                                                                                                    |                                                                                                                                                     
tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                     <  2:1189686/150‑1 (MQ=255)
   accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATGAAatt                                                                                                                                                  >  2:88763/1‑150 (MQ=255)
    ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                                 >  1:951383/1‑150 (MQ=255)
    ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                                 <  1:1510996/150‑1 (MQ=255)
    ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAaata                                                                                                                                                 >  2:4012664/1‑150 (MQ=255)
    ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAatta                                                                                                                                                 >  1:302916/1‑150 (MQ=255)
       ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                              <  1:2208827/150‑1 (MQ=255)
          cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                           <  1:5297568/150‑1 (MQ=255)
          cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTAtttttt                                                                                                                                           >  2:2739891/1‑150 (MQ=255)
           ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                          <  2:1830426/150‑1 (MQ=255)
            tGCATCGTTTCGAGATTGTGAATATTGACTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTAGATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                                         <  2:5481352/150‑1 (MQ=255)
             gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATTAAAATATtttttttt                                                                                                                                        >  2:2636311/1‑150 (MQ=255)
             gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGATTTGAAAATATTTTTTAAtttttttttttt                                                                                                                                        >  2:2227075/1‑150 (MQ=255)
              cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                       <  2:5768617/150‑1 (MQ=255)
               aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                      <  1:4470895/150‑1 (MQ=255)
               aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                      >  2:2435824/1‑150 (MQ=255)
                tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATAAAATGAAATTATTTTTTTTTtaa                                                                                                                                     >  1:1658216/1‑150 (MQ=255)
                 cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAat                                                                                                                                    <  2:1324379/150‑1 (MQ=255)
                   tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAatat                                                                                                                                  >  2:5588836/1‑150 (MQ=255)
                   tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                                  >  2:4992767/1‑150 (MQ=255)
                     tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTTAAatatag                                                                                                                                >  2:5165738/1‑148 (MQ=255)
                         gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                            <  1:2546020/150‑1 (MQ=255)
                         gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                            <  1:5430681/150‑1 (MQ=255)
                            tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                         <  1:291935/150‑1 (MQ=255)
                              tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAAGAAATTAATTGTTTTTAAAAATCGAGATAACGa                                                                                                                       >  1:4727812/1‑150 (MQ=255)
                               gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                      >  2:4537470/1‑150 (MQ=255)
                                  tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGTTTTAAATATATAATTTAATTTTTTTTTTTTTAATATATAGATAAAGATaac                                                                                                                   >  1:5743782/1‑150 (MQ=255)
                                  tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                                   <  1:2703828/150‑1 (MQ=255)
                                  tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTACGAATTAAAATAAAAAAAAAATATTTTTTTTTAAATATAAACATAAcaaaaac                                                                                                                   >  1:1017568/1‑144 (MQ=255)
                                   atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                                  <  1:1096340/150‑1 (MQ=255)
                                     tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTTAATATCGAGAGAACGATCACaaa                                                                                                                >  1:3682348/1‑150 (MQ=255)
                                       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  2:1219210/150‑1 (MQ=255)
                                       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAAAATCGAGATAACGATCACaaaaa                                                                                                              >  1:3182784/1‑150 (MQ=255)
                                       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTGTTTTTAGATATAAAAAAATAAATAAAATATTTTTTTTTAAATATAGAGATAACGAAAACaaaaa                                                                                                              >  1:5612019/1‑150 (MQ=255)
                                        aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                             <  1:2558519/150‑1 (MQ=255)
                                          gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATGAAAATTTTTGTTTTTAAATATAGCGATAACGCTAACCAAAACGa                                                                                                           >  1:2729447/1‑150 (MQ=255)
                                           ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                          <  2:567856/150‑1 (MQ=255)
                                           ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                          <  2:568094/150‑1 (MQ=255)
                                            cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAATAAAATATTTTTTTTTTAATATCGAGCTAAAGATAACAAAAAAGACa                                                                                                         >  1:523815/1‑150 (MQ=255)
                                             ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGTTAACGATCACAAAAACGACaa                                                                                                        >  1:1341363/1‑150 (MQ=255)
                                                aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAAAGATAACAAAAACGACAaaat                                                                                                     >  1:4738899/1‑150 (MQ=255)
                                                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAATATAATTTAATTATTTTTTTTTTATTATATAGTTTAAGATGACAAAAAATACAaaattta                                                                                                  >  1:1933797/1‑147 (MQ=255)
                                                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATTTAATTATTTTTTTTTTAATTTAGAGATAAAGATAACAAAAAAGACAATATTaa                                                                                                  >  1:1933700/1‑150 (MQ=255)
                                                    cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                                 <  2:358263/150‑1 (MQ=255)
                                                    cATAACCGCCGCGCTGTGCGGTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGCTTTCAAAAAAAAATGAAATTATTTTTTTTTAAATATCGAGATAACCATCACAAAAACGACAAAATGaca                                                                                                 >  2:1663581/1‑148 (MQ=255)
                                                         ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATAGAGATAACCATCACAAAAAAGAAAATAAGAAAattat                                                                                            >  2:2093089/1‑150 (MQ=255)
                                                            ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTAGATTTAAAAATAAAAAGAAAATTTTTGTTTTTTTATATAGAGATAAAGATCACAAAAAAGACAAAATGAAAATTATTCg                                                                                         >  1:1432425/1‑150 (MQ=255)
                                                              gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                                       <  2:5052470/150‑1 (MQ=255)
                                                                gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAAAGAAAATATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAATATGTAAAATATTCgagga                                                                                     >  2:3947321/1‑150 (MQ=255)
                                                                   gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACACTATGAAAATTATTCGCGGAGTg                                                                                  >  2:1353323/1‑150 (MQ=255)
                                                                      cGTTTTTCAGAATCTGCTTTGTCGATACCACGTACATTTTCATAGCCTGTCTTTTTTTTTCGTTTTCAAAATATAATGAAATTATTTTTTTTTAATTATCGATATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                               <  1:3075272/150‑1 (MQ=255)
                                                                      cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                               <  2:2494824/150‑1 (MQ=255)
                                                                        ttgttCAGCATCTGCTTGGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                             <  2:721893/150‑1 (MQ=255)
                                                                           ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAAAATTAAAATTATTAGAGGAGTGAAAGGcca                                                                          >  2:3025706/1‑148 (MQ=255)
                                                                               gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCCTTTGTTTTCGAATACAAAATATAAATAAATTATTTGTTTTTAAATATCGAGAGAAACAACACAAAAAAGACAATAATAAAATTATTAGAGGAGTGAAAGGCaaaaaa                                                                      >  2:2714140/1‑150 (MQ=255)
                                                                                cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                     >  2:2926584/1‑150 (MQ=255)
                                                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                                   <  2:610372/150‑1 (MQ=255)
                                                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAAAGAAAATATTTTTTTTTTAATATAGAGAGAACGATAAAAAAAAACACAAAATAAAAAATAATAGATGAGGGTAAGGCaaaaaaaac                                                                   >  1:3491463/1‑148 (MQ=255)
                                                                                   cTGCTTTGTCGATACCACGTACATTTTCATATCCTTTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                                  <  2:2654782/150‑1 (MQ=255)
                                                                                   cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                                  <  2:3199839/150‑1 (MQ=255)
                                                                                      cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                                               <  2:5798716/150‑1 (MQ=255)
                                                                                      cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                                               <  2:4673696/150‑1 (MQ=255)
                                                                                       tttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                              <  2:2702940/150‑1 (MQ=255)
                                                                                           tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                          <  2:341870/150‑1 (MQ=255)
                                                                                             taTACCACGAAAATTTTAATATCCTTTCGTTTTTTTTATTTTTAAAAATTTAATTAATTTTTTTTTTTTTAAATATCGAGAAAACGATAAAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                        <  1:4793821/149‑1 (MQ=255)
                                                                                                   aCGTAATTTTTCATATCCTTTTTTTTGTTTTCTATTAAAAAATATAATGAAATTTTTTTTTTTTAAATTTCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                  <  1:4955241/150‑1 (MQ=255)
                                                                                                      tACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                               <  2:5587040/150‑1 (MQ=255)
                                                                                                      tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                               <  2:2817150/150‑1 (MQ=255)
                                                                                                      tACATTTAAATATCATCTCTTTTGATTTAGATTTCAAAATATAATTAAATTATTTGATTATAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCg                                               <  2:3281883/150‑1 (MQ=255)
                                                                                                       aCATTTTCATTTCCTGTCGTTTTTTTTCGATTACAAAATATAATGAATTTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                                              <  1:3052309/150‑1 (MQ=255)
                                                                                                        cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAGGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTa                                             <  2:1713636/150‑1 (MQ=255)
                                                                                                             tCATATCGTGTCTTTTTTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTAAAATATCGATATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                        <  1:5721433/150‑1 (MQ=255)
                                                                                                             tCATATCCTGTCTTTTGTTTACTATTAAAAAATATAATTAATTTTTTTTTTTTAAAATATCGATAAACCTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                        <  1:1975215/150‑1 (MQ=255)
                                                                                                             tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATCATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                        <  2:5268845/150‑1 (MQ=255)
                                                                                                                 atCCTGTCTTTTGTTTTCTATTAAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                    <  1:4567536/150‑1 (MQ=255)
                                                                                                                   ccTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                  <  1:3796180/150‑1 (MQ=255)
                                                                                                                   ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                  <  2:784166/150‑1 (MQ=255)
                                                                                                                     tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                >  2:421638/1‑150 (MQ=255)
                                                                                                                       tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                              >  2:44427/1‑150 (MQ=255)
                                                                                                                          tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           >  2:4314412/1‑150 (MQ=255)
                                                                                                                          tttgtttTCGATTTCAAAATATAATGAAATTATTTCTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           >  1:3955104/1‑150 (MQ=255)
                                                                                                                            ttttttCGATTTAAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                         <  1:5118736/148‑1 (MQ=255)
                                                                                                                              ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:2412837/1‑150 (MQ=255)
                                                                                                                               tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAATTTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                      >  1:2815760/1‑150 (MQ=255)
                                                                                                                               tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                      >  2:3682891/1‑150 (MQ=255)
                                                                                                                                 tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    <  2:5467122/150‑1 (MQ=255)
                                                                                                                                       ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  1:3352678/1‑150 (MQ=255)
                                                                                                                                        tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              <  1:3539076/150‑1 (MQ=255)
                                                                                                                                        tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              >  2:3577149/1‑150 (MQ=255)
                                                                                                                                         cAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             <  1:546969/150‑1 (MQ=255)
                                                                                                                                         cAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCATAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             <  1:4245795/150‑1 (MQ=255)
                                                                                                                                          aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            <  1:1437230/150‑1 (MQ=255)
                                                                                                                                           aaaTATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           <  1:2644253/150‑1 (MQ=255)
                                                                                                                                            aaTATATTGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt          <  1:1806867/150‑1 (MQ=255)
                                                                                                                                            aaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt          <  1:742517/150‑1 (MQ=255)
                                                                                                                                               ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       <  2:4873471/150‑1 (MQ=255)
                                                                                                                                                aaaTCAAATTATTTTTTTATAAATATCGACAAAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      <  1:2227075/149‑1 (MQ=255)
                                                                                                                                                 aaTGAAATAATTTGTTTTTAAATATCGAGTTATCGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa     <  1:4001538/150‑1 (MQ=255)
                                                                                                                                                   tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa   >  2:3160076/1‑150 (MQ=255)
                                                                                                                                                    gAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  <  2:1109842/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177098‑2177394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: