Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,275 C→A 6.2% intergenic (‑71/+237) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2750CA 6.2% 808.9 / 4.0 252intergenic (‑71/+237)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (90/136);  new base A (24/2);  total (114/138)
Fisher's exact test for biased strand distribution p-value = 2.03e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

TGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGAC  >  NC_000913/2177126‑2177423
                                                                                                                                                       |                                                                                                                                                    
   gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGAt                                                                                                                                                   >  2:4474141/1‑150 (MQ=255)
      tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGTTTTAAATATATAATTTAATTTTTTTTTTTTTAATATATAGATAAAGATaac                                                                                                                                                >  1:5743782/1‑150 (MQ=255)
      tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTACGAATTAAAATAAAAAAAAAATATTTTTTTTTAAATATAAACATAAcaaaaac                                                                                                                                                >  1:1017568/1‑144 (MQ=255)
        ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATAGAGGTAACGATCACaa                                                                                                                                              >  1:1845537/1‑150 (MQ=255)
        ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                              <  2:4338555/150‑1 (MQ=255)
           aaTGCCGGAACCGTATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAAAAAAATTTAATTATTTTTTTTTAAATATAGAGATAAAGATAACaaaaa                                                                                                                                           >  1:2817150/1‑150 (MQ=255)
           aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                           <  2:1167111/150‑1 (MQ=255)
           aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                           <  2:1219210/150‑1 (MQ=255)
           aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                           <  2:47433/150‑1 (MQ=255)
            aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                                                          <  1:2558520/150‑1 (MQ=255)
              gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATGAAAATTTTTGTTTTTAAATATAGCGATAACGCTAACCAAAACGa                                                                                                                                        >  1:2729447/1‑150 (MQ=255)
               ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                                                       <  2:567856/150‑1 (MQ=255)
               ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATCGAGATAACGATCACAAAAACGAc                                                                                                                                       >  2:1333059/1‑150 (MQ=255)
                cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAATAAAATATTTTTTTTTTAATATCGAGCTAAAGATAACAAAAAAGACa                                                                                                                                      >  1:523815/1‑150 (MQ=255)
                 ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATGAAATTATTTGTTTTTTAATATAGAGATAAAGATCACAAAAAAGACaa                                                                                                                                     >  1:5455902/1‑150 (MQ=255)
                 ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGTTAACGATCACAAAAACGACaa                                                                                                                                     >  1:1341363/1‑150 (MQ=255)
                  gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTCAAAATATAAATAAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGACaaa                                                                                                                                    >  1:742887/1‑149 (MQ=255)
                      cGCATAACCGCCGCGCTGTGCGTTTTTCAGAATGTGCTTTGTCTATACAACGTACTTTTTCATATCATTTCTTTTGTTTTCTATTTAAAAATATATTGAAATTATTTGTTTTTAATTATCTTGATAACGATCACAAAAACGACAATATGa                                                                                                                                <  2:3927628/150‑1 (MQ=255)
                       gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATAAAAATAAAGTATTTTTTTTTTAATTTAGAGATAAACATAACCAAAAAGACAaaattta                                                                                                                               >  1:3970623/1‑147 (MQ=255)
                        cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                                                              <  2:358263/150‑1 (MQ=255)
                        cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAACGACAAAATTaaa                                                                                                                              >  2:1697249/1‑150 (MQ=255)
                        cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGCTTTCAAAATATAATGAAATTATTTTTTTTTTAATATCGAGATAACGATCACACAAACGACAATATGtaa                                                                                                                              >  2:4572747/1‑150 (MQ=255)
                           aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAATAAATTATTTTTTTTTAAATAAAGAGATAAAGATAACAAAAAAGACAAAAATAAAatt                                                                                                                           >  2:2669967/1‑150 (MQ=255)
                                 cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                                                     <  2:426519/150‑1 (MQ=255)
                                  gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                                                                    <  2:5052256/150‑1 (MQ=255)
                                               ttCAGCTTCTGCTTTGTCGAAACCACTTACTTTTTAATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATAAAATTTTTTTTTTTTAAATATTGTCATAACTATAACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                                                                       <  1:306235/150‑1 (MQ=255)
                                               ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAAAATTAAAATTATTAGAGGAGTGAAAGGcca                                                                                                       >  2:3025706/1‑148 (MQ=255)
                                               ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACGATAAAAAAAAAGACAATATGAAAATTATTAGAGGGGTGAAAGGCaa                                                                                                       >  2:71596/1‑150 (MQ=255)
                                                tCAGAATCTGCTTTGTCTAAACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTAAAAAATATAATTAATTTATTTTTTTTTAAATATCGATATACCCAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                                                      <  1:44577/150‑1 (MQ=255)
                                                     aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATTAAATTATTTTTTTTTAAATATAGAGATAACGAAAACAAAAAATACAATATGAAAATTATTCGATGAGTGTAATGCaaaaaaaa                                                                                                 >  2:187544/1‑149 (MQ=255)
                                                       cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                                                               <  2:3199839/150‑1 (MQ=255)
                                                          cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                                                                            <  2:5798716/150‑1 (MQ=255)
                                                          cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                                                                            <  2:4673696/150‑1 (MQ=255)
                                                            ttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGATGAGTGAAAGGCAAAAAAACGGCCTcc                                                                                          >  2:5175679/1‑150 (MQ=255)
                                                              gTCGATACCACGAAATTTTTCATTTCCTGTCTTTTTTTTTCTTTTAAAAAATATATTTAAATTATTTTTTTTTAAATTTGTTTATAACGATAAAAAAAACGACAATTTTAAATTTTTTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCg                                                                                        <  1:3890928/150‑1 (MQ=255)
                                                               tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                       <  2:341870/150‑1 (MQ=255)
                                                               tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCCAAAAAAAGGCCACCCGa                                                                                       >  2:5587764/1‑150 (MQ=255)
                                                               tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAAATATTTTTTTTTAAATATAGAGATAACGATAACAAAAAACACAAAAAGAAAATTATTCGAGGGGTGGAAGGCAAAAAAACGGCCTCCTGa                                                                                       >  2:2703411/1‑150 (MQ=255)
                                                               tCGAAACAACTTAAATTTTATTATCCTGTTTTTTTTTTTATTTTAAAAAATATAATAAATTTTTTTTTTTTAAATTATCTATATAACTAACAAAAAAACTAAAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                       <  1:4635438/150‑1 (MQ=255)
                                                                 gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                                                     >  2:5707182/1‑150 (MQ=255)
                                                                  aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAATTATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                                                                    <  2:1715780/150‑1 (MQ=255)
                                                                      cACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                                <  1:3134231/150‑1 (MQ=255)
                                                                       aCGTAATTTTTCATATCCTTTTTTTTGTTTTCTATTAAAAAATATAATGAAATTTTTTTTTTTTAAATTTCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                                               <  1:4955241/150‑1 (MQ=255)
                                                                          tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                                                            <  2:2817150/150‑1 (MQ=255)
                                                                          tACATTTAAATATCATCTCTTTTGATTTAGATTTCAAAATATAATTAAATTATTTGATTATAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCg                                                                            <  2:3281883/150‑1 (MQ=255)
                                                                           aaaTTTTCATCACCTGTCTTTTTTTTTCGATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                                                                           <  1:4758287/148‑1 (MQ=255)
                                                                                 tCATATCCTGTCTTTTGTTTACTATTAAAAAATATAATTAATTTTTTTTTTTTAAAATATCGATAAACCTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                                     <  1:1975215/150‑1 (MQ=255)
                                                                                     atCCTGTCTTTTGTTTTCTATTAAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                                                 <  1:4567536/150‑1 (MQ=255)
                                                                                       cgtGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTTTTTGTTTTTAATTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                                               <  1:2318026/148‑1 (MQ=255)
                                                                                       ccTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                                               <  1:3796180/150‑1 (MQ=255)
                                                                                         tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcgg                                                             >  2:5275262/1‑149 (MQ=255)
                                                                                             gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                         >  2:2242794/1‑150 (MQ=255)
                                                                                                ttttttCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                                                      <  1:4735471/148‑1 (MQ=255)
                                                                                                ttttttCGATTTAAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                                                      <  1:5118736/148‑1 (MQ=255)
                                                                                                  ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                    >  1:2412837/1‑150 (MQ=255)
                                                                                                   tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                   >  2:3682891/1‑150 (MQ=255)
                                                                                                   tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                   >  1:2142825/1‑150 (MQ=255)
                                                                                                   tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                   >  1:1043059/1‑150 (MQ=255)
                                                                                                    ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                                                  >  2:946283/1‑150 (MQ=255)
                                                                                                       cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                                                >  2:4885460/1‑150 (MQ=255)
                                                                                                         aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                                              <  1:3678796/150‑1 (MQ=255)
                                                                                                         aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGCCGACCCGAAGGGGACCCCTTAGCAAGGTGCGCGGGTTTTTTTGCCGGAt                                              >  1:5645154/1‑150 (MQ=255)
                                                                                                           ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                            >  2:1380143/1‑150 (MQ=255)
                                                                                                           ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                            >  1:3352678/1‑150 (MQ=255)
                                                                                                           ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                            >  1:2708446/1‑150 (MQ=255)
                                                                                                           ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                            <  2:765828/150‑1 (MQ=255)
                                                                                                            tCAAAATATAATGAAATTATTTGTTTTTAAATATCTAGATAACGATCACATATAGGTCTATATGAATATTATTCGAGGTGTGAAAGGCAATAAAACTGCCTCCCGATAGGGAAGCCGTAGCAATGTGCGCGTGTTTTTATGGCGGATGCg                                           >  1:1616754/1‑150 (MQ=255)
                                                                                                            tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                           >  2:3577149/1‑150 (MQ=255)
                                                                                                             cAAAATATACTGAAATTATTTGTTTTTAAATATCGAGATAACGACCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                          <  2:2540009/150‑1 (MQ=255)
                                                                                                             cAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCATAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                          <  1:4245795/150‑1 (MQ=255)
                                                                                                              aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                         <  1:1437229/150‑1 (MQ=255)
                                                                                                              aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                         <  1:304864/150‑1 (MQ=255)
                                                                                                               aaaTATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                        <  1:2644253/150‑1 (MQ=255)
                                                                                                                aaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                       <  1:742503/150‑1 (MQ=255)
                                                                                                                 atatatTGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa                                      <  1:5587764/150‑1 (MQ=255)
                                                                                                                   ataATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                    <  2:5249971/150‑1 (MQ=255)
                                                                                                                    taATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc                                   <  2:3963369/150‑1 (MQ=255)
                                                                                                                     aaTGAAATTATTTGTATTAAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa                                  <  1:5409694/150‑1 (MQ=255)
                                                                                                                       tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                >  2:3160076/1‑150 (MQ=255)
                                                                                                                        gAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                               <  2:1109842/150‑1 (MQ=255)
                                                                                                                            ttattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTgg                           <  1:888210/150‑1 (MQ=255)
                                                                                                                               tttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAg                        >  2:3728148/1‑150 (MQ=255)
                                                                                                                               tttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAg                        >  2:2012340/1‑150 (MQ=255)
                                                                                                                                ttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGt                       >  2:588710/1‑150 (MQ=255)
                                                                                                                                ttGTTTATAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGt                       <  2:5496052/150‑1 (MQ=255)
                                                                                                                                  gTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGc                     <  1:171145/150‑1 (MQ=255)
                                                                                                                                   tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCt                    >  2:3306389/1‑150 (MQ=255)
                                                                                                                                     tttAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt                  <  1:4792006/150‑1 (MQ=255)
                                                                                                                                     tttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt                  >  1:4809623/1‑150 (MQ=255)
                                                                                                                                       tAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGt                >  2:2848124/1‑150 (MQ=255)
                                                                                                                                            tatCGAGATAACGATCACAAAAACGACAATATGAAAATTGTTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc            <  1:3511834/150‑1 (MQ=255)
                                                                                                                                            tatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc            <  1:1353323/150‑1 (MQ=255)
                                                                                                                                             atCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa           <  2:409912/150‑1 (MQ=255)
                                                                                                                                             atCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa           <  1:2926584/150‑1 (MQ=255)
                                                                                                                                             atCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa           >  1:3641197/1‑150 (MQ=255)
                                                                                                                                              tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGTTAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt          >  2:2934840/1‑150 (MQ=255)
                                                                                                                                              tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt          <  1:2435824/150‑1 (MQ=255)
                                                                                                                                                     aaCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa   >  1:3294616/1‑150 (MQ=255)
                                                                                                                                                     aaCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa   >  1:345806/1‑150 (MQ=255)
                                                                                                                                                      aCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGAc  >  2:249313/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
TGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGAC  >  NC_000913/2177126‑2177423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: