Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,279 C→A 6.8% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2790CA 6.8% 772.5 / 4.2 254intergenic (‑75/+233)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (97/127);  new base A (20/9);  total (118/136)
Fisher's exact test for biased strand distribution p-value = 1.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

TATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTC  >  NC_000913/2177132‑2177428
                                                                                                                                                   |                                                                                                                                                     
tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGTTTTAAATATATAATTTAATTTTTTTTTTTTTAATATATAGATAAAGATaac                                                                                                                                                     >  1:5743782/1‑150 (MQ=255)
tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                                                                     <  1:2703828/150‑1 (MQ=255)
 atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                                                                    <  1:1096340/150‑1 (MQ=255)
  ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGCTAAAGATCACaa                                                                                                                                                   >  2:3643216/1‑150 (MQ=255)
     aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                                <  2:5487115/150‑1 (MQ=255)
     aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                                <  2:1219210/150‑1 (MQ=255)
     aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                                <  2:47433/150‑1 (MQ=255)
     aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAAAATCGAGATAACGATCACaaaaa                                                                                                                                                >  1:3182784/1‑150 (MQ=255)
      aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                                                               <  1:2558519/150‑1 (MQ=255)
        gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATGAAAATTTTTGTTTTTAAATATAGCGATAACGCTAACCAAAACGa                                                                                                                                             >  1:2729447/1‑150 (MQ=255)
          cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAATAAAATATTTTTTTTTTAATATCGAGCTAAAGATAACAAAAAAGACa                                                                                                                                           >  1:523815/1‑150 (MQ=255)
           ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATGAAATTATTTGTTTTTTAATATAGAGATAAAGATCACAAAAAAGACaa                                                                                                                                          >  1:5455902/1‑150 (MQ=255)
           ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGTAAATATTTTTTTTTTAATATCGAGGTGAAGCTAAAAAAAAAGACaa                                                                                                                                          >  1:2963809/1‑150 (MQ=255)
           ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACaa                                                                                                                                          >  1:1342042/1‑150 (MQ=255)
            gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTCAAAATATAAATAAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGACaaa                                                                                                                                         >  1:742887/1‑149 (MQ=255)
              aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAAAGATAACAAAAACGACAaaat                                                                                                                                       >  1:4738899/1‑150 (MQ=255)
                 gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAATATAATTTAATTATTTTTTTTTTATTATATAGTTTAAGATGACAAAAAATACAaaattta                                                                                                                                    >  1:1933797/1‑147 (MQ=255)
                  cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGCTTTCAAAATATAATGAAATTATTTTTTTTTTAATATCGAGATAACGATCACACAAACGACAATATGtaa                                                                                                                                   >  2:4572747/1‑150 (MQ=255)
                  cATAACCGCCGCGCTGTGCGGTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGCTTTCAAAAAAAAATGAAATTATTTTTTTTTAAATATCGAGATAACCATCACAAAAACGACAAAATGaca                                                                                                                                   >  2:1663581/1‑148 (MQ=255)
                   aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACCAAAACGACAAAATGgaaa                                                                                                                                  >  2:2972689/1‑150 (MQ=255)
                     aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAATAAATTATTTTTTTTTAAATAAAGAGATAAAGATAACAAAAAAGACAAAAATAAAatt                                                                                                                                >  2:2669967/1‑150 (MQ=255)
                       ccgccgCGCTGTGCGTTGTTAAGCATCTGCTTTGTCGATACAACGTACATTTTCATATCATGTCGTTTGTTTTAGAATTAAAAATATAATGAAATAATTTTTTTTTTAATATAGATAAAAAGAACACAAAAAAGACAAAATTAAAaatag                                                                                                                              >  1:3761621/1‑149 (MQ=255)
                          ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTAGATTTAAAAATAAAAAGAAAATTTTTGTTTTTTTATATAGAGATAAAGATCACAAAAAAGACAAAATGAAAATTATTCg                                                                                                                           >  1:1432425/1‑150 (MQ=255)
                           cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                                                          <  2:426519/150‑1 (MQ=255)
                            gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                                                                         <  2:5052470/150‑1 (MQ=255)
                                tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACTTAAATTTTAATATCCTGTCTTTTGTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGt                                                                                                                     <  1:3300727/150‑1 (MQ=255)
                                    cGTTTTTCAGAATCTGCTTTGTCGATACCACGTACATTTTCATAGCCTGTCTTTTTTTTTCGTTTTCAAAATATAATGAAATTATTTTTTTTTAATTATCGATATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                 <  1:3075272/150‑1 (MQ=255)
                                         ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACCACAAAATGAAAAATAATCGAGGCGTGGAAGGCaa                                                                                                            >  2:4123874/1‑150 (MQ=255)
                                         ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACGATAAAAAAAAAGACAATATGAAAATTATTAGAGGGGTGAAAGGCaa                                                                                                            >  2:71596/1‑150 (MQ=255)
                                             gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCCTTTGTTTTCGAATACAAAATATAAATAAATTATTTGTTTTTAAATATCGAGAGAAACAACACAAAAAAGACAATAATAAAATTATTAGAGGAGTGAAAGGCaaaaaa                                                                                                        >  2:2714140/1‑150 (MQ=255)
                                                tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAAAGAAAATATTTTTTTTTTAATATAGAGAGAACGATAAAAAAAAACACAAAATAAAAAATAATAGATGAGGGTAAGGCaaaaaaaac                                                                                                     >  1:3491463/1‑148 (MQ=255)
                                                    cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                                                                                 <  2:4673696/150‑1 (MQ=255)
                                                         tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                            <  2:341870/150‑1 (MQ=255)
                                                         tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCCAAAAAAAGGCCACCCGa                                                                                            >  2:5587764/1‑150 (MQ=255)
                                                           taTACCACGAAAATTTTAATATCCTTTCGTTTTTTTTATTTTTAAAAATTTAATTAATTTTTTTTTTTTTAAATATCGAGAAAACGATAAAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                                                          <  1:4793821/149‑1 (MQ=255)
                                                           gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                                                          >  2:5707182/1‑150 (MQ=255)
                                                            aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAATTATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                                                                         <  2:1715780/150‑1 (MQ=255)
                                                                 aCGTAATTTTTCATATCCTTTTTTTTGTTTTCTATTAAAAAATATAATGAAATTTTTTTTTTTTAAATTTCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                                                    <  1:4955241/150‑1 (MQ=255)
                                                                   gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                                                  <  2:1162978/150‑1 (MQ=255)
                                                                    tACATTTAAATATCATCTCTTTTGATTTAGATTTCAAAATATAATTAAATTATTTGATTATAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCg                                                                                 <  2:3281883/150‑1 (MQ=255)
                                                                     aCATTTTCATTTCCTGTCGTTTTTTTTCGATTACAAAATATAATGAATTTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                                                                                <  1:3052309/150‑1 (MQ=255)
                                                                           tcttatCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                                          <  1:1697249/147‑1 (MQ=255)
                                                                           tCATATCCTGTGGTTTGTTTTCTATTTCAAAATTTCATGACATTATTTGTTTTTAAATATCGAGATAACGTTCACCAAAACGACACTATGAAAATTATTCGAGGAGTGAAAGGCAATAATACTGGCTCCCGATAGGGAAGCCGTAGCaaa                                                                          <  2:5268851/150‑1 (MQ=255)
                                                                           tCATATCCTGTCTTTTGTTTTCGATTACAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                                          <  1:3841334/150‑1 (MQ=255)
                                                                               atCCTGTCGTTTGTTTTCTATTTCAAAATATAATAAAATTATTTGTTTTTAAATATCTATATAACGAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                                                      <  1:4567575/150‑1 (MQ=255)
                                                                                tCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                                                                     >  2:4356649/1‑150 (MQ=255)
                                                                                      cGTTTGTTTTCTATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                                                               <  1:3643216/150‑1 (MQ=255)
                                                                                       gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                              >  2:2242794/1‑150 (MQ=255)
                                                                                        tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                                                             >  1:2427689/1‑150 (MQ=255)
                                                                                          tgtttTCGATTTCAAAATATATTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                                                           <  1:4780935/150‑1 (MQ=255)
                                                                                          tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                                                           <  2:5624820/150‑1 (MQ=255)
                                                                                             tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAATTTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                        >  1:2815760/1‑150 (MQ=255)
                                                                                             tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                        >  2:3682891/1‑150 (MQ=255)
                                                                                              ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                                                       >  2:946283/1‑150 (MQ=255)
                                                                                               gcGATTCCAAAAGATAAGGAAATTTTTTGTTTTTAAATATCGAGATACCGATCACCAACACGACAATATGAAAATTATTCGCGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                                                      <  1:5111952/149‑1 (MQ=255)
                                                                                                cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                                                     >  2:4885460/1‑150 (MQ=255)
                                                                                                  aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                                                   >  2:2845511/1‑150 (MQ=255)
                                                                                                  aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGCCGACCCGAAGGGGACCCCTTAGCAAGGTGCGCGGGTTTTTTTGCCGGAt                                                   >  1:5645154/1‑150 (MQ=255)
                                                                                                    ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                                 <  2:765828/150‑1 (MQ=255)
                                                                                                    ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                                 >  2:1380143/1‑150 (MQ=255)
                                                                                                     tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                <  1:3539076/150‑1 (MQ=255)
                                                                                                     tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                >  1:2559052/1‑150 (MQ=255)
                                                                                                     tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                >  2:4026999/1‑150 (MQ=255)
                                                                                                     tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                >  2:3577149/1‑150 (MQ=255)
                                                                                                      cAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCATAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                               <  1:4245795/150‑1 (MQ=255)
                                                                                                      cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                               <  1:3432116/150‑1 (MQ=255)
                                                                                                      cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGGGTTTTTATGCCGGATGCgg                                               >  2:2630683/1‑150 (MQ=255)
                                                                                                       aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                              <  1:1437230/150‑1 (MQ=255)
                                                                                                         aaTATATTGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                            <  1:1806867/150‑1 (MQ=255)
                                                                                                         aaTATACTGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                            <  1:742512/150‑1 (MQ=255)
                                                                                                         aaTATAAGGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                            <  2:2175987/150‑1 (MQ=255)
                                                                                                          atatatTGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa                                           <  1:5587764/150‑1 (MQ=255)
                                                                                                          ataaAATTAAATTATTTTTTTAAAAATATATACATAAATATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa                                           <  1:4473751/150‑1 (MQ=255)
                                                                                                            ataATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                         <  2:5249971/150‑1 (MQ=255)
                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc                                        <  2:3963369/150‑1 (MQ=255)
                                                                                                              aaTGAAATTATTTGTATTAAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa                                       <  1:5409694/150‑1 (MQ=255)
                                                                                                              aaTGAAATAATTTGTTTTTAAATATCGAGTTATCGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa                                       <  1:4001538/150‑1 (MQ=255)
                                                                                                                tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                     >  2:3160076/1‑150 (MQ=255)
                                                                                                                tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                     >  2:3205729/1‑150 (MQ=255)
                                                                                                                 gAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                                    <  2:1109842/150‑1 (MQ=255)
                                                                                                                        tttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAg                             >  2:3728148/1‑150 (MQ=255)
                                                                                                                         ttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATTCCGGATGCGGTGTAAAAAAACGTGGCAGt                            >  1:2000857/1‑150 (MQ=255)
                                                                                                                         ttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGt                            >  2:1276275/1‑150 (MQ=255)
                                                                                                                         ttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTGTTATGCCGGATGCGGTGTAATCATGCGTGGCAGt                            >  2:3788771/1‑150 (MQ=255)
                                                                                                                         ttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGt                            >  2:588710/1‑150 (MQ=255)
                                                                                                                          tttttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                           <  1:3946973/148‑1 (MQ=255)
                                                                                                                          tGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                           >  1:2359098/1‑150 (MQ=255)
                                                                                                                            tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCt                         <  2:1104762/150‑1 (MQ=255)
                                                                                                                            tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCt                         >  2:5098350/1‑150 (MQ=255)
                                                                                                                            tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCt                         >  2:3306389/1‑150 (MQ=255)
                                                                                                                                tAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGt                     <  2:302916/150‑1 (MQ=255)
                                                                                                                                tAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGt                     >  2:1897818/1‑150 (MQ=255)
                                                                                                                                  aaTATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAg                   <  1:1035621/150‑1 (MQ=255)
                                                                                                                                  aaTATCGAGATAACGATCACAAAAACGACACGATGAAAATTATTCGAGGAGTGAAAGGCAAAACAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAg                   <  2:2921991/150‑1 (MQ=255)
                                                                                                                                           aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa          <  2:5749359/150‑1 (MQ=255)
                                                                                                                                              aCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGAc       >  2:249313/1‑150 (MQ=255)
                                                                                                                                                gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc     >  1:2169009/1‑150 (MQ=255)
                                                                                                                                                gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc     >  1:4645771/1‑150 (MQ=255)
                                                                                                                                                 aTCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCt    >  1:2886213/1‑150 (MQ=255)
                                                                                                                                                   cacaAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTc  <  2:1845537/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTC  >  NC_000913/2177132‑2177428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: