Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,575,877 A→C 6.4% intergenic (‑72/+221) eutS ← / ← maeB putative ethanol utilization carboxysome structural protein/malic enzyme: putative oxidoreductase/phosphotransacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,575,8770AC 6.4% 966.2 / 10.4 338intergenic (‑72/+221)eutS/maeBputative ethanol utilization carboxysome structural protein/malic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base A (124/182);  new base C (27/3);  total (152/186)
Fisher's exact test for biased strand distribution p-value = 1.01e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 345 total aligned reads displayed.

ACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATA  >  NC_000913/2575728‑2576026
                                                                                                                                                     |                                                                                                                                                     
  cGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAgc                                                                                                                                                     <  2:2126683/150‑1 (MQ=255)
    ggATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAgcgc                                                                                                                                                   <  2:737466/150‑1 (MQ=255)
    ggATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAgcgc                                                                                                                                                   <  1:4386958/150‑1 (MQ=255)
      aTGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGt                                                                                                                                                 <  1:1369865/150‑1 (MQ=255)
          gcgATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTaa                                                                                                                                             <  1:5686899/150‑1 (MQ=255)
           cgATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTaaa                                                                                                                                            <  2:3701901/150‑1 (MQ=255)
           cgATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTaaa                                                                                                                                            <  2:3525260/150‑1 (MQ=255)
               gagaTGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGt                                                                                                                                        <  1:1989533/150‑1 (MQ=255)
                 gaTGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGa                                                                                                                                      <  2:1356552/150‑1 (MQ=255)
                 gaTGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGa                                                                                                                                      <  1:2796551/150‑1 (MQ=255)
                  aTGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAg                                                                                                                                     <  2:3655944/150‑1 (MQ=255)
                  aTGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAg                                                                                                                                     <  1:3027897/150‑1 (MQ=255)
                   tGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAgg                                                                                                                                    <  2:4534131/150‑1 (MQ=255)
                    gcgcCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGt                                                                                                                                   <  1:4219209/150‑1 (MQ=255)
                       ccAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCACGCGCGTTTAAAAGGTGAGGTAGa                                                                                                                                >  1:4632664/1‑150 (MQ=255)
                        cAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGAt                                                                                                                               <  1:1110837/150‑1 (MQ=255)
                         aGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACACAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATc                                                                                                                              >  1:5464981/1‑150 (MQ=255)
                          gtgtGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCa                                                                                                                             <  2:535705/150‑1 (MQ=255)
                              gACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTaa                                                                                                                         >  2:3970707/1‑150 (MQ=255)
                                ccTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGa                                                                                                                       >  2:4862227/1‑150 (MQ=255)
                                  tGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAAt                                                                                                                     <  2:393282/150‑1 (MQ=255)
                                       gCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCACGCGCGGTTAAAAGGTGAGGTAGATCACTAAGAATGgaga                                                                                                                >  2:2997016/1‑150 (MQ=255)
                                          gggCACAAATTCCTGTATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGGGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCACGCGCGTGTATAAGGTGCGGTAGATCACTAAGAGGGGAGAGat                                                                                                             >  1:2423825/1‑150 (MQ=255)
                                           ggCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGata                                                                                                            >  2:1185166/1‑150 (MQ=255)
                                               caAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCg                                                                                                        <  1:5097366/150‑1 (MQ=255)
                                                aaaTTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGc                                                                                                       >  1:72358/1‑150 (MQ=255)
                                                aaaTTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGc                                                                                                       >  1:1255838/1‑150 (MQ=255)
                                                aaaTTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGc                                                                                                       >  2:3860951/1‑150 (MQ=255)
                                                  aTTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCtg                                                                                                     <  1:4875768/150‑1 (MQ=255)
                                                      cTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTgtggt                                                                                                 <  1:1540006/150‑1 (MQ=255)
                                                      cTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTgtggt                                                                                                 >  2:1641374/1‑150 (MQ=255)
                                                       tGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCACGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTgtggtg                                                                                                >  1:380010/1‑150 (MQ=255)
                                                       tGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTgtggtg                                                                                                >  2:2684016/1‑150 (MQ=255)
                                                        gactgatgCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAACAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGc                                                                                               <  2:17424/147‑1 (MQ=255)
                                                        gAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGc                                                                                               >  2:4442056/1‑150 (MQ=255)
                                                        gAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGc                                                                                               >  1:1456089/1‑150 (MQ=255)
                                                         aaTGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCa                                                                                              <  1:1923205/150‑1 (MQ=255)
                                                          atgatgCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCAt                                                                                             <  1:1728281/150‑1 (MQ=255)
                                                          atgatgCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCAt                                                                                             <  1:4986698/150‑1 (MQ=255)
                                                          atgatgCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCAt                                                                                             >  1:3403093/1‑150 (MQ=255)
                                                             atgCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATaaa                                                                                          <  2:684470/150‑1 (MQ=255)
                                                                cGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACtt                                                                                       <  1:2255514/150‑1 (MQ=255)
                                                                   tatttATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATAGTGACATTTTTTCGTCAGTGTAAATAACATAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCa                                                                                    <  2:3293053/148‑1 (MQ=255)
                                                                   tCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCTTGACAATTTTTCGTCAGATTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCa                                                                                    <  1:3711673/150‑1 (MQ=255)
                                                                   tCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCa                                                                                    <  1:2935458/150‑1 (MQ=255)
                                                                    cTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCat                                                                                   <  2:1191062/150‑1 (MQ=255)
                                                                    cTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCat                                                                                   <  2:3143917/150‑1 (MQ=255)
                                                                    cTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCat                                                                                   <  2:2498370/150‑1 (MQ=255)
                                                                     tttATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCCCGCGCGTTTAAAAGGTGAGGTAGATCACTCAGAAGGGAGAGATATTCGCTGTGGTGCAGAAACTTTCata                                                                                  >  2:2313629/1‑150 (MQ=255)
                                                                     tttATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAAGAACAAAATTCGGCACGCGCGTTGAAAAGGTGAGGTCGAGCACTAAGAATGGAGAGATATTCGCTGTGGTGCCTAAACGTTCcta                                                                                  >  1:982532/1‑150 (MQ=255)
                                                                      ttATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCACGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCatat                                                                                 >  2:4821768/1‑150 (MQ=255)
                                                                      ttATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCatat                                                                                 <  2:434781/150‑1 (MQ=255)
                                                                       tATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCCGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGCGAGATATTCGCGGTGGTGCCTAAACTTTCATATg                                                                                >  1:5350938/1‑150 (MQ=255)
                                                                          ccATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCaa                                                                             <  1:2612590/150‑1 (MQ=255)
                                                                            aTGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACg                                                                           >  1:3074665/1‑150 (MQ=255)
                                                                             tGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGc                                                                          <  2:1546236/150‑1 (MQ=255)
                                                                                 tCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGa                                                                      <  2:141231/150‑1 (MQ=255)
                                                                                     cgccTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAatat                                                                  <  1:4577563/150‑1 (MQ=255)
                                                                                      gccTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCCACGCATGAAtata                                                                 >  1:5015287/1‑150 (MQ=255)
                                                                                       ccTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATataa                                                                <  2:1020996/150‑1 (MQ=255)
                                                                                           tgAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATataaataa                                                            <  1:583414/150‑1 (MQ=255)
                                                                                             aaaaaCTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATaaataaaa                                                          >  1:883295/1‑150 (MQ=255)
                                                                                             aaaaaCTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATaaataaaa                                                          <  1:5574534/150‑1 (MQ=255)
                                                                                             aaaaaCTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATaaataaaa                                                          >  2:2753508/1‑150 (MQ=255)
                                                                                              aaaaCTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATaaataaaat                                                         >  1:3933625/1‑150 (MQ=255)
                                                                                                  cTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAataata                                                     <  1:2335089/150‑1 (MQ=255)
                                                                                                   tttCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGACTGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAatat                                                    >  2:580462/1‑150 (MQ=255)
                                                                                                     tCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCg                                                  <  2:2595062/150‑1 (MQ=255)
                                                                                                      cGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCga                                                 <  2:2983821/150‑1 (MQ=255)
                                                                                                       gtgtATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCgag                                                <  1:2113658/150‑1 (MQ=255)
                                                                                                           aTCGTGAAAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGatat                                            <  2:4945979/150‑1 (MQ=255)
                                                                                                               tGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATtata                                        <  2:1255838/150‑1 (MQ=255)
                                                                                                               tGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATtata                                        <  2:5504242/150‑1 (MQ=255)
                                                                                                                gACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCCAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATtatat                                       >  1:4488504/1‑150 (MQ=255)
                                                                                                                  cAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAg                                     >  1:102082/1‑150 (MQ=255)
                                                                                                                   aaTTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGa                                    >  1:3192477/1‑150 (MQ=255)
                                                                                                                    aTTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGAc                                   >  2:2014434/1‑150 (MQ=255)
                                                                                                                        ttCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCt                               >  2:1084537/1‑150 (MQ=255)
                                                                                                                           gTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttt                            >  1:758848/1‑150 (MQ=255)
                                                                                                                            tCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCttttt                           >  2:5743081/1‑150 (MQ=255)
                                                                                                                            tCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCttttt                           >  2:4200837/1‑150 (MQ=255)
                                                                                                                            tCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCttttt                           >  1:4732566/1‑150 (MQ=255)
                                                                                                                               gagTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttttttt                        >  2:2139471/1‑150 (MQ=255)
                                                                                                                               gagTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttttttt                        <  1:1121822/150‑1 (MQ=255)
                                                                                                                                agTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTtta                       <  2:1938314/150‑1 (MQ=255)
                                                                                                                                  tAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTttatt                     <  2:5609138/150‑1 (MQ=255)
                                                                                                                                  tAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTttatt                     >  2:4704289/1‑150 (MQ=255)
                                                                                                                                  tAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTttatt                     <  2:5402884/150‑1 (MQ=255)
                                                                                                                                      tAACAAACTTCGGCACGCGCGTTTACAAGGTGAGGTAGTTCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTc                 <  1:3327177/150‑1 (MQ=255)
                                                                                                                                      tAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTc                 <  2:160890/150‑1 (MQ=255)
                                                                                                                                        aCAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGc               <  1:2498383/150‑1 (MQ=255)
                                                                                                                                          aaaaTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCaa             >  2:3374689/1‑150 (MQ=255)
                                                                                                                                          aaaaTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCaa             >  2:3569057/1‑150 (MQ=255)
                                                                                                                                           aaaTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCaaa            <  1:4970402/150‑1 (MQ=255)
                                                                                                                                             aTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACt          >  1:3638748/1‑150 (MQ=255)
                                                                                                                                             aTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACt          >  2:2896197/1‑150 (MQ=255)
                                                                                                                                               tCGGCACGCGCGATTCCCAGGTGAGGTAGATCACTAAGACTGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATCTCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCa        <  2:3408855/150‑1 (MQ=255)
                                                                                                                                                  gCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGta     >  2:5564925/1‑150 (MQ=255)
                                                                                                                                                    aaGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGtaat   >  2:2480857/1‑150 (MQ=255)
                                                                                                                                                     aGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGtaata  <  2:5053301/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCGGGATGCGCGATGAGATGCGCCAGTGTGACCTGTTTGCCGGGCACAAATTCCTGAATGATGCGTTCTTTATCCATGAGTCGCCGCCTGTGAAAAACTTTCGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATA  >  NC_000913/2575728‑2576026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: