Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,637,433 C→T 5.4% intergenic (‑77/+41) der ← / ← bamB GTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,637,4330CT 5.4% 1281.8 / 38.1 371intergenic (‑77/+41)der/bamBGTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (184/165);  new base T (15/5);  total (200/171)
Fisher's exact test for biased strand distribution p-value = 6.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 387 total aligned reads displayed.

TGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCA  >  NC_000913/2637284‑2637582
                                                                                                                                                     |                                                                                                                                                     
tGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGcc                                                                                                                                                       <  2:1073480/150‑1 (MQ=255)
  cGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGTGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGcccc                                                                                                                                                     >  2:4410685/1‑150 (MQ=255)
  cGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGcccc                                                                                                                                                     >  1:5227244/1‑150 (MQ=255)
      ttAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTc                                                                                                                                                 <  2:825380/150‑1 (MQ=255)
      ttAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTc                                                                                                                                                 <  2:3497522/150‑1 (MQ=255)
            ggTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAg                                                                                                                                           <  2:4778464/150‑1 (MQ=255)
             gTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGc                                                                                                                                          >  1:3613377/1‑150 (MQ=255)
              ttAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGcc                                                                                                                                         >  1:4048610/1‑150 (MQ=255)
                  aTAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCTCCTGGACAGGGGCCGttt                                                                                                                                     <  2:2332984/150‑1 (MQ=255)
                  aTAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGttt                                                                                                                                     >  1:5186770/1‑150 (MQ=255)
                    aaCGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGATATATTCGCGTCGTTGTTAAAAACAGTAAAACGGCTCCTGGACAGGGGCCGTTTTc                                                                                                                                   <  2:72623/150‑1 (MQ=255)
                      ggTGGATTTTCCTACGTTCGGGCGCCCCACACGCGCGACCACCGGTACCTTTTTTAACGCCTAATTTTTATAAATCATCAGCCACCGCCCGCTTTTTGCGGGTGTTTTTTAAAAACAGTAAAACGGCCCCTGTCCAGGAGCCGTTTTCaa                                                                                                                                 <  1:700705/149‑1 (MQ=255)
                       gTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                                <  2:5234490/150‑1 (MQ=255)
                       gTGGATGGTCCTACGTTAGGGCGCCCGACAAGCGCGACCACCGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                                <  2:5688017/150‑1 (MQ=255)
                            ttttCCTCCGTTAGGGCGCCCGCCCCGCGCGACCCCAGGTACCAGGTTTAACGCCTCATTTTTATAAATCATCAGCCACCGCCCGCTATATTCGCGTCGTTGTTAAAAACAGGAACACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGaa                                                                                                                           <  1:5458972/150‑1 (MQ=255)
                            ttttCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCACACACCGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGaa                                                                                                                           <  1:5674981/150‑1 (MQ=255)
                                ccTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGAc                                                                                                                       <  2:3356818/150‑1 (MQ=255)
                                  tACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGGTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACag                                                                                                                     >  2:5468158/1‑150 (MQ=255)
                                   aCGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACaga                                                                                                                    <  2:5016597/150‑1 (MQ=255)
                                       tAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACg                                                                                                                >  2:4935576/1‑150 (MQ=255)
                                           gcgcCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTa                                                                                                            >  1:5173415/1‑150 (MQ=255)
                                            cgcCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTaa                                                                                                           <  2:384467/150‑1 (MQ=255)
                                             gcCCGACAAGCGCGACCACAGTTACATTTTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCTTTTTTAAAAACAGTAAAACGGCCCCTGTCCTGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAc                                                                                                          <  1:782338/150‑1 (MQ=255)
                                             gcCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAc                                                                                                          <  2:2034959/150‑1 (MQ=255)
                                               ccGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgt                                                                                                        >  1:2608181/1‑150 (MQ=255)
                                                cGACAAGCGCGACCACAGGTACATTTTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTTTTAAAAACAGTAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgtg                                                                                                       <  1:1716354/150‑1 (MQ=255)
                                                 gACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgtgt                                                                                                      <  2:4587128/150‑1 (MQ=255)
                                                 gACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgtgt                                                                                                      >  2:1451756/1‑150 (MQ=255)
                                                 gACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgtgt                                                                                                      >  1:35022/1‑150 (MQ=255)
                                                 gACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgagt                                                                                                      >  2:912345/1‑150 (MQ=255)
                                                     cgcgcGACCACTGGTACCTTGTTTAAAGCCTCATTTTTATAAATAATCACACAACCCACGCTATATTCGCGTCTTTGTTAAAAACAGCAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATa                                                                                                  <  1:4887296/149‑1 (MQ=255)
                                                     aGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATa                                                                                                  <  2:1587629/150‑1 (MQ=255)
                                                     aGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATa                                                                                                  <  2:4498694/150‑1 (MQ=255)
                                                     aGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATa                                                                                                  <  2:5414257/150‑1 (MQ=255)
                                                       cgcgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATaga                                                                                                <  2:2158767/150‑1 (MQ=255)
                                                       cgcgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATaga                                                                                                >  1:2842279/1‑150 (MQ=255)
                                                         cgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGt                                                                                              >  1:3364834/1‑150 (MQ=255)
                                                          gACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTa                                                                                             >  1:3975966/1‑150 (MQ=255)
                                                          gACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTa                                                                                             >  1:2714686/1‑150 (MQ=255)
                                                             caccGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTacac                                                                                          >  2:321140/1‑150 (MQ=255)
                                                             cacaGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTacac                                                                                          >  1:468703/1‑150 (MQ=255)
                                                                aGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGt                                                                                       <  2:3287704/150‑1 (MQ=255)
                                                                  gTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTc                                                                                     >  1:1388952/1‑150 (MQ=255)
                                                                     ccATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGt                                                                                  >  1:4322202/1‑150 (MQ=255)
                                                                      cATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTc                                                                                 >  1:3770233/1‑150 (MQ=255)
                                                                       aTGTTTAAAGCCTCATTTTTATAATTCATCAGCCAACGCACGCTCTATTCGCGTCGTTGTTAAACACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACCGAGACGATTAACGTGTTATAGAGTACACGGTTCCGTCt                                                                                <  1:1161992/150‑1 (MQ=255)
                                                                              aaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCt                                                                         >  2:2643945/1‑150 (MQ=255)
                                                                                 ccTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGa                                                                      >  1:2039037/1‑150 (MQ=255)
                                                                                 ccTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGa                                                                      >  2:1482063/1‑150 (MQ=255)
                                                                                  cTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGAt                                                                     <  2:1250824/150‑1 (MQ=255)
                                                                                   tCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATATAGTACACGGTTCCGTCTTTTGCCTGGATc                                                                    >  1:5407081/1‑150 (MQ=255)
                                                                                   tCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATc                                                                    >  2:1212089/1‑150 (MQ=255)
                                                                                         ttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGt                                                              <  2:2388075/150‑1 (MQ=255)
                                                                                          tataAATCATCACACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTTTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGACTACACCGTTCCGTCTTTTGCCTGGATCAGCAGtt                                                             >  2:19714/1‑150 (MQ=255)
                                                                                             aaaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGc                                                          >  1:3826988/1‑150 (MQ=255)
                                                                                              aaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAACCAGGAAAACGGCCCCTGTCCAGGATCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGTGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGcc                                                         <  1:5636936/150‑1 (MQ=255)
                                                                                              aaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATCGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGcc                                                         <  2:3714252/150‑1 (MQ=255)
                                                                                              aaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGcc                                                         <  1:179282/150‑1 (MQ=255)
                                                                                              aaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGcc                                                         <  1:2330891/150‑1 (MQ=255)
                                                                                                  atcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCa                                                     <  2:4856031/150‑1 (MQ=255)
                                                                                                   tcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAg                                                    >  2:1165908/1‑150 (MQ=255)
                                                                                                      gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCCGTTTGCCGTCAGCgg                                                 >  1:1011893/1‑150 (MQ=255)
                                                                                                      gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCgg                                                 >  2:1385412/1‑150 (MQ=255)
                                                                                                      gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCgg                                                 >  2:529279/1‑150 (MQ=255)
                                                                                                      gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCgg                                                 >  2:2245704/1‑150 (MQ=255)
                                                                                                      gACAACGCACGCTATATTCGCGTCGTTCTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGATTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGATCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCgg                                                 >  2:1671111/1‑150 (MQ=255)
                                                                                                       aCAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGc                                                >  1:5040604/1‑150 (MQ=255)
                                                                                                        cAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCa                                               >  1:3694451/1‑150 (MQ=255)
                                                                                                          acgcacgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAAc                                             <  2:5607350/150‑1 (MQ=255)
                                                                                                          acgcacgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAAc                                             <  2:4110309/150‑1 (MQ=255)
                                                                                                               cgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGtt                                        >  1:4280771/1‑150 (MQ=255)
                                                                                                                gcTATATTCGCGTCGTTGTTAAAAACCGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTc                                       <  2:3485820/150‑1 (MQ=255)
                                                                                                                gcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTc                                       <  2:2749075/150‑1 (MQ=255)
                                                                                                                gcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTc                                       <  1:1957083/150‑1 (MQ=255)
                                                                                                                 cTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCa                                      <  1:1463881/150‑1 (MQ=255)
                                                                                                                   atagTCGCGTCGTTGTTAAAAACAGGAACACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGt                                    <  2:5780635/150‑1 (MQ=255)
                                                                                                                    tatTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTaca                                                                                           <  1:241556/94‑1 (MQ=255)
                                                                                                                     atTCGCGTCGTTGTTAAAAACAGGAACACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTACTAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCTGTTTGCCGTCAGCGGCAACCGGTTCAGTCt                                  <  2:1936514/150‑1 (MQ=255)
                                                                                                                     atTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCt                                  >  2:1617513/1‑150 (MQ=255)
                                                                                                                            tcgtTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAAcc                           <  2:556793/150‑1 (MQ=255)
                                                                                                                            tcgtTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTAAACGGTTCCGTCTTTTTCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAAcc                           >  1:5561414/1‑150 (MQ=255)
                                                                                                                             ctttgttAAAATCAGGAAAACTGACCCTGTCCAGGAGCCGTTTTCTATGTGAACTACAGAGACGATTAACGTGTAATAGTGTACACTGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCg                          <  1:2093551/148‑1 (MQ=255)
                                                                                                                              gttgttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCgg                         <  1:2954031/150‑1 (MQ=255)
                                                                                                                               ttgttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGa                        >  1:107828/1‑150 (MQ=255)
                                                                                                                               ttgttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGa                        <  1:4814975/150‑1 (MQ=255)
                                                                                                                               ttgttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGa                        <  2:4305953/150‑1 (MQ=255)
                                                                                                                                tgttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGaa                       <  2:2264664/150‑1 (MQ=255)
                                                                                                                                  ttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACt                     >  2:3964355/1‑150 (MQ=255)
                                                                                                                                    aaaaaCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTAt                   <  2:117347/150‑1 (MQ=255)
                                                                                                                                      aaaCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCa                 <  2:5139821/150‑1 (MQ=255)
                                                                                                                                      aaaCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCa                 >  2:3102146/1‑150 (MQ=255)
                                                                                                                                        ctttgAAAACGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAAc               >  1:4203851/5‑150 (MQ=255)
                                                                                                                                              aaaCGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTg         >  2:50576/1‑150 (MQ=255)
                                                                                                                                               aaCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGc        <  1:3230825/150‑1 (MQ=255)
                                                                                                                                                aCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTTCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGCACTATCAACTTTTTGCt       >  1:5717267/1‑150 (MQ=255)
                                                                                                                                                aCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCt       <  1:4601554/150‑1 (MQ=255)
                                                                                                                                                 cGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTg      <  2:1829007/150‑1 (MQ=255)
                                                                                                                                                  ggCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTgg     <  2:3613377/150‑1 (MQ=255)
                                                                                                                                                  ggCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTgg     <  2:3399752/150‑1 (MQ=255)
                                                                                                                                                     cccTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCGTTTGCCGGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCa  >  1:5820457/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCA  >  NC_000913/2637284‑2637582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: