Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,173 C→T 5.3% P116L (CCG→CTG)  yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,1730CT 5.3% 976.4 / 26.8 286P116L (CCG→CTG) yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base C (138/132);  new base T (8/7);  total (146/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 290 total aligned reads displayed.

ATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCT  >  NC_000913/3368025‑3368322
                                                                                                                                                    |                                                                                                                                                     
aTCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCg                                                                                                                                                      >  1:2316090/1‑150 (MQ=255)
  cAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCa                                                                                                                                                    <  1:2690522/150‑1 (MQ=255)
  cAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCa                                                                                                                                                    <  1:2402103/150‑1 (MQ=255)
  cAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCa                                                                                                                                                    <  1:1405077/150‑1 (MQ=255)
   aaGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCCACATGTTGAAATTCACCAGACAATGTCTGGCGCATTAACACCATTGGGACTTCCGCAg                                                                                                                                                   >  1:4181000/1‑150 (MQ=255)
   aaGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAg                                                                                                                                                   >  2:3600831/1‑150 (MQ=255)
     gCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGac                                                                                                                                                 >  1:938998/1‑150 (MQ=255)
     gCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGac                                                                                                                                                 >  2:521000/1‑150 (MQ=255)
      cGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGaca                                                                                                                                                <  1:3640709/150‑1 (MQ=255)
             cAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCTACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTg                                                                                                                                         >  2:5108850/1‑150 (MQ=255)
              aaaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCg                                                                                                                                                      <  1:677543/136‑1 (MQ=255)
              aaaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGc                                                                                                                                        >  2:5018462/1‑150 (MQ=255)
               aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                       >  1:3080745/1‑150 (MQ=255)
               aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                       >  2:2815507/1‑150 (MQ=255)
               aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                       <  2:3232704/150‑1 (MQ=255)
               aaGGATTTATGTCGAAGGTTTAAGCGTGGGTTCTCTGCAAAAAACTTCCGCCATATGAGGCTTTTTTACCTCTTTTTTCAACCTGTTGAAATTCCCCAGACACTGTCTGGCGAATTAACACCCTTGGGACTTCCGCAGACACCGTCTGCa                                                                                                                                       <  2:5670092/150‑1 (MQ=255)
                aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAACTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGcgg                                                                                                                                      <  1:4072199/150‑3 (MQ=255)
                aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGcgg                                                                                                                                      <  1:859378/150‑3 (MQ=255)
                aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAg                                                                                                                                      >  1:744866/1‑150 (MQ=255)
                aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAg                                                                                                                                      >  1:4265625/1‑150 (MQ=255)
                 ggATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGa                                                                                                                                     >  2:5581045/1‑150 (MQ=255)
                 ggATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGa                                                                                                                                     >  1:3923842/1‑150 (MQ=255)
                 ggATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTGTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGACTTCACACCATTGGGAATTACGCAGACACCGTCTGCAGa                                                                                                                                     >  2:3838735/1‑150 (MQ=255)
                  gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                    <  1:4424179/150‑1 (MQ=255)
                    tttATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAAtt                                                                                                                                  <  1:147144/150‑1 (MQ=255)
                       aTGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTccc                                                                                                                               >  2:3519548/1‑150 (MQ=255)
                         gTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCAt                                                                                                                             <  1:4427966/150‑1 (MQ=255)
                          tCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATc                                                                                                                            >  2:4010743/1‑150 (MQ=255)
                           cTAAGGTATCAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCg                                                                                                                           <  2:246729/150‑1 (MQ=255)
                            tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCgg                                                                                                                          >  1:1864010/1‑150 (MQ=255)
                            tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCgg                                                                                                                          <  2:4110142/150‑1 (MQ=255)
                            tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCgg                                                                                                                          >  2:1252411/1‑150 (MQ=255)
                             aaGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGc                                                                                                                         >  1:5493974/1‑150 (MQ=255)
                              aGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTGTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGACTTCCCCTCGGCa                                                                                                                        >  1:5542834/1‑150 (MQ=255)
                               ggTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaa                                                                                                                       <  2:5493974/150‑1 (MQ=255)
                                 tataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAg                                                                                                                     >  1:25773/1‑150 (MQ=255)
                                 tataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTGTTACCTCTTTTTTCAACCTGTTGAAATTCACCAGACAATGTCTGGCGAAGTAACACCATTGGGAATTCCGCAGACACCGGCTGCAGAATTCCCCTCGGCAAAg                                                                                                                     >  2:2425887/1‑150 (MQ=255)
                                     aGCGTGGTTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAttt                                                                                                                 <  1:5124679/150‑1 (MQ=255)
                                     aGCGTGGGTTCTCTTCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATTTTGAATTTCACCTGACAATGTCTGGCGAATTAACACCTTTGGGATTTCCGCAGACACCGTCTGCTGAATTCCCATCGGCAAAGAttt                                                                                                                 <  1:2541831/150‑1 (MQ=255)
                                     aGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAttt                                                                                                                 <  2:4854893/150‑1 (MQ=255)
                                         tGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTTAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCa                                                                                                             >  1:5220364/1‑150 (MQ=255)
                                           ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCCTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGa                                                                                                           <  1:5645040/150‑1 (MQ=255)
                                                tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTg                                                                                                      <  2:1476990/150‑1 (MQ=255)
                                                  tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                    <  1:3987885/150‑1 (MQ=255)
                                                  tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                    >  1:5694877/1‑150 (MQ=255)
                                                  tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                    >  2:1183533/1‑150 (MQ=255)
                                                     aaaaaaCTTACTCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                 <  2:1021035/150‑1 (MQ=255)
                                                     aaaaaaCTTACGCCACATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                 <  1:1270872/150‑1 (MQ=255)
                                                     aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACAcc                                                                                                                                              >  2:1753546/1‑105 (MQ=255)
                                                       aaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCa                                                                                               <  2:2316090/150‑1 (MQ=255)
                                                               cGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTc                                                                                       <  2:842983/150‑1 (MQ=255)
                                                                gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                      <  2:5046164/150‑1 (MQ=255)
                                                                gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCATTcc                                                                                      >  1:5216738/1‑150 (MQ=255)
                                                                gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAAGTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                      <  2:1934166/150‑1 (MQ=255)
                                                                 ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTccc                                                                                     >  2:217641/1‑150 (MQ=255)
                                                                    aaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCtt                                                                                  <  2:1864955/150‑1 (MQ=255)
                                                                        tggCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCAGTGGGAATTCCGCAGACACCGTCTGCAGAAGTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTGTCCCCTTGccc                                                                              >  1:3799854/2‑150 (MQ=255)
                                                                          gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCCTCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                            >  2:875028/1‑150 (MQ=255)
                                                                              ttttACCTCTTTTTACAACCTGTGGAAATTCACCCGACAATGTCGGGCGAATGAACACCAATGGGAATTCCGCAGACACCGTCTGCAGATTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTcc                                                                        <  1:1386763/150‑1 (MQ=255)
                                                                                ttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                                      <  2:2026592/150‑1 (MQ=255)
                                                                                ttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                                      <  2:4119805/150‑1 (MQ=255)
                                                                                 tACCTGTTTTTTCAACTTTTTGAAATTCACCAGACATTGTCTGGCGAATTAACACCTTGGGTATTTCGGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAcc                                                                     <  1:4920527/150‑1 (MQ=255)
                                                                                         ttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTg                                                                                                                <  1:4659238/99‑1 (MQ=255)
                                                                                          tttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCAGCGGCAAAGATTTGGCTGACGCTGTCTGGCAAATCTTTCCCCTTGCCCCGGTCCACCGACGTCCgg                                                            >  1:4140321/1‑149 (MQ=255)
                                                                                            tCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCTACGTCCGttt                                                          >  2:3154182/1‑150 (MQ=255)
                                                                                            tCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAGTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCCCATCTTTCCCCGTGCCCCGGTCCCCCTCCGGCCGgtt                                                          >  1:2083497/1‑150 (MQ=255)
                                                                                             cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTa                                                         <  2:1606695/150‑1 (MQ=255)
                                                                                                cATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACtt                                                      <  2:2265203/150‑1 (MQ=255)
                                                                                                   gTTGAAATTCACCCGACAATGTCTGGAGAATTAACACCATTGGGTATTCCGCAGACACCGTCTTCAGAATTCACATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                   <  2:3311046/150‑1 (MQ=255)
                                                                                                        aaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaa                                              <  2:3923842/150‑1 (MQ=255)
                                                                                                         aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                             <  1:3375248/150‑1 (MQ=255)
                                                                                                           tCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTTGCATTAGCTGTCTGGCAAATCTTTACCATTGCCCAGGTCCACCTAAGTCCGTTTTCTTTCCGTTaaaaa                                           >  1:1007594/1‑150 (MQ=255)
                                                                                                             aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAAGTCCGTTTACTTTCCGTTAAAAACg                                         >  1:5061948/1‑150 (MQ=255)
                                                                                                              ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCCCCGCCCGCCGTGTAATTGCCGGTAAAAACGc                                        >  1:5399990/1‑150 (MQ=255)
                                                                                                                 gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGa                                     >  1:3577056/1‑150 (MQ=255)
                                                                                                                   cAAGGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACg                                   <  1:3062050/150‑1 (MQ=255)
                                                                                                                      tGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                <  1:4335334/150‑1 (MQ=255)
                                                                                                                      tGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTACCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                >  2:693867/1‑150 (MQ=255)
                                                                                                                       gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAACGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCg                               <  1:5299721/150‑1 (MQ=255)
                                                                                                                       gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCg                               >  2:2641678/1‑150 (MQ=255)
                                                                                                                       gTCTGGAGAATTAAAACCATTGGGAAGTCCGCATACACGGTGTGAAGATTTCCAATCTGAAAAGATTTGGCAGACGCTGTCTGCAAAATCTTTCCCCTTGCCCAGTCCAACCTACTTCCGTTTAATTTCCTTTAAAAACGCTGACGCCCg                               <  1:1749281/150‑1 (MQ=255)
                                                                                                                          tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTGTGCCAAAACATTACCCTTGCCCAGGTCCACCAACGTCCGTTTACTTTGCGTTAAAAACGCGGACCCCCGCAg                            >  1:2004736/1‑150 (MQ=255)
                                                                                                                            gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCt                          >  2:2949514/1‑150 (MQ=255)
                                                                                                                              gAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGTCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTTCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCtgt                        >  2:5613409/1‑148 (MQ=255)
                                                                                                                               aaTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCATAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCtttt                       <  1:3439757/150‑1 (MQ=255)
                                                                                                                               aaTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCtttt                       <  2:1806239/150‑1 (MQ=255)
                                                                                                                                       ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa               <  2:4910297/150‑1 (MQ=255)
                                                                                                                                       ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa               <  2:4806135/150‑1 (MQ=255)
                                                                                                                                       ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa               <  1:2939943/150‑1 (MQ=255)
                                                                                                                                       ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa               <  1:1336646/150‑1 (MQ=255)
                                                                                                                                       ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa               <  2:3981104/150‑1 (MQ=255)
                                                                                                                                        cATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAag              >  2:4182997/1‑150 (MQ=255)
                                                                                                                                        cATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAag              <  1:1111582/150‑1 (MQ=255)
                                                                                                                                          ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGGTAAAAACGCTGACGCCCGCAGCTTTGAGGAAAAagag            >  2:2989126/1‑150 (MQ=255)
                                                                                                                                          ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAATTTGCAGAAGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCAACCTACCGTTACTTTACGTTAAAAAAGCATACGCCCCCAGCATTTTTGTAAAagag            >  1:2245484/1‑150 (MQ=255)
                                                                                                                                            gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGAc          >  2:631453/1‑150 (MQ=255)
                                                                                                                                             ggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACg         <  2:4304269/150‑1 (MQ=255)
                                                                                                                                                 ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTCTGCCCAATCTTTACCCTAGCCCAGGTCCACCGACGTCCGTTTAAATTCCGGTAAAAACGCGGACGCCCGCAGCTTTTATGAACAAGAGACGCTcc     >  1:1200529/1‑150 (MQ=255)
                                                                                                                                                 ttCTGCAGACAGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc     >  2:2517640/1‑150 (MQ=255)
                                                                                                                                                    cGCAGACACCGTCGGCAGAATTCCCCTCGGCAAAGATTTGGCAGACGCTGTCTGCCAAAACTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTACCGTAAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt  <  1:2731222/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCT  >  NC_000913/3368025‑3368322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: