Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,182 C→T 5.4% P119L (CCG→CTG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,1820CT 5.4% 937.6 / 26.8 276P119L (CCG→CTG) ‡yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base C (129/131);  new base T (8/7);  total (137/139)
Fisher's exact test for biased strand distribution p-value = 7.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 280 total aligned reads displayed.

ACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGG  >  NC_000913/3368033‑3368330
                                                                                                                                                     |                                                                                                                                                    
aCTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACAcc                                                                                                                                                      >  2:1754628/1‑150 (MQ=255)
      aaaGGATTTATGTCTACGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGc                                                                                                                                                <  1:5554413/150‑1 (MQ=255)
      aaaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGc                                                                                                                                                >  2:5165484/1‑150 (MQ=255)
       acggATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCTCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                               <  2:3938597/148‑1 (MQ=255)
       aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                               >  2:2815507/1‑150 (MQ=255)
       aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                               >  2:2197840/1‑150 (MQ=255)
       aaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                                               >  1:3080745/1‑150 (MQ=255)
       aaGGATTTATGTCGAAGGTTTAAGCGTGGGTTCTCTGCAAAAAACTTCCGCCATATGAGGCTTTTTTACCTCTTTTTTCAACCTGTTGAAATTCCCCAGACACTGTCTGGCGAATTAACACCCTTGGGACTTCCGCAGACACCGTCTGCa                                                                                                                                               <  2:5670092/150‑1 (MQ=255)
        aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAACTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGcgg                                                                                                                                              <  1:4072199/150‑3 (MQ=255)
        aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCCGACAATGTCTGGCGCATTAACACCATTGGGAATTCCGCTGACACCGTCTGCAg                                                                                                                                              >  1:5730412/1‑150 (MQ=255)
        aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGcgg                                                                                                                                              <  1:859337/150‑3 (MQ=255)
        aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAg                                                                                                                                              >  1:4265080/1‑150 (MQ=255)
         ggATTTATTTCTAAGGTATAAGCGTGGGTTCTCAGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCAGTGGGAATTCCGCAGACACCGTCTGCAGa                                                                                                                                             >  2:3595764/1‑150 (MQ=255)
         ggATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGa                                                                                                                                             >  2:5581045/1‑150 (MQ=255)
          gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                                            <  1:4424179/150‑1 (MQ=255)
            tttATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAAtt                                                                                                                                          <  1:2897321/150‑1 (MQ=255)
                 gTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCAt                                                                                                                                     <  1:4427966/150‑1 (MQ=255)
                  tCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATc                                                                                                                                    >  2:4010743/1‑150 (MQ=255)
                  tCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATc                                                                                                                                    >  1:1398559/1‑150 (MQ=255)
                  tCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAAGGTCTGGCGAATTAACACCATTGGGACTTCCGCAGACACCGTCTGCAGAATTCCCATc                                                                                                                                    >  1:2283400/1‑150 (MQ=255)
                   cTAAGGTATCAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCg                                                                                                                                   <  2:246729/150‑1 (MQ=255)
                    tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCgg                                                                                                                                  >  2:1252411/1‑150 (MQ=255)
                       ggTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaa                                                                                                                               <  2:5493974/150‑1 (MQ=255)
                        gTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGATATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaaa                                                                                                                              <  2:5026887/150‑1 (MQ=255)
                          ataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGa                                                                                                                            >  1:150137/1‑150 (MQ=255)
                            aaGCGTGGGTTCTCTGCAAAAAACTGACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGCCAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAtt                                                                                                                          <  2:1516466/150‑1 (MQ=255)
                             cgCGTGGGTTCTCTGAAAAAAACTTACGCCAATTGAGGCTTTTTTACCTCTTTTTTCAACATGTTTAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCATAATTCCCATCGGCAAAGAttt                                                                                                                         <  1:5650544/149‑1 (MQ=255)
                             aGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAttt                                                                                                                         <  2:4854893/150‑1 (MQ=255)
                               cGTGGGTTCTCTGCAAACAACTTACGCCTAATGCGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACATTGTCTGGCGATTTAACACCTTTGGTAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTgg                                                                                                                       <  1:259509/150‑1 (MQ=255)
                                 tGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTTAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCa                                                                                                                     >  1:5220364/1‑150 (MQ=255)
                                     ttCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACg                                                                                                                 >  2:2081796/1‑150 (MQ=255)
                                        tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTg                                                                                                              <  2:1476990/150‑1 (MQ=255)
                                          tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                            <  1:3987885/150‑1 (MQ=255)
                                          tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                            <  1:5006344/150‑1 (MQ=255)
                                          tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                            >  2:1183533/1‑150 (MQ=255)
                                           gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCt                                                                                                           <  2:1670205/150‑1 (MQ=255)
                                            cAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTcgg                                                                                                          >  1:717743/1‑148 (MQ=255)
                                             aaaaaaCTTACTCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                         <  2:1021035/150‑1 (MQ=255)
                                             aaaaaaCTTACGCCACATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                         <  1:1270872/150‑1 (MQ=255)
                                              aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGcc                                                                                                        >  1:1946179/1‑150 (MQ=255)
                                                aaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaa                                                                                                      <  2:5102243/150‑1 (MQ=255)
                                                 aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaaa                                                                                                     <  1:2725816/150‑1 (MQ=255)
                                                 aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaaa                                                                                                     <  1:5476671/150‑1 (MQ=255)
                                                    ttACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCt                                                                                                  >  2:1815421/1‑150 (MQ=255)
                                                      aCGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCttt                                                                                                <  2:4143788/150‑1 (MQ=255)
                                                       cGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTc                                                                                               <  2:842983/150‑1 (MQ=255)
                                                         ccAAATGAGGCTTTTTTACCTCTTTTTTCAAAAAGTTTAAATTCACCAGACAATGTCTGTCGAATTAACAACAATGTGAATTACTCAGACACCCACAGCCGAAATCACAGCGGCAAATATTTTGCAGACGCTGTGTTCCAAAtaaataac                                                                                             >  1:2572411/1‑143 (MQ=255)
                                                           aaaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTACCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCt                                                                                           >  1:3956242/1‑150 (MQ=255)
                                                                tggCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCAGTGGGAATTCCGCAGACACCGTCTGCAGAAGTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTGTCCCCTTGccc                                                                                      >  1:3799854/2‑150 (MQ=255)
                                                                  gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCCTCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                    >  2:875028/1‑150 (MQ=255)
                                                                        ttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                                              <  2:2026592/150‑1 (MQ=255)
                                                                          aCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCt                                                                            >  1:552795/1‑150 (MQ=255)
                                                                            ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                          >  2:3443208/1‑150 (MQ=255)
                                                                              ctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGt                                                                        >  2:3217162/1‑150 (MQ=255)
                                                                               ttttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTc                                                                                                                                         <  2:3685100/84‑1 (MQ=255)
                                                                                tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTcc                                                                      <  2:3113847/150‑1 (MQ=255)
                                                                                tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTcc                                                                      >  1:2222438/1‑150 (MQ=255)
                                                                                 ttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTg                                                                                                                        <  1:4659238/99‑1 (MQ=255)
                                                                                 ttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTg                                                                                                                        <  1:1750809/99‑1 (MQ=255)
                                                                                 ttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTg                                                                                                                        >  2:4659238/1‑99 (MQ=255)
                                                                                  tttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCAGCGGCAAAGATTTGGCTGACGCTGTCTGGCAAATCTTTCCCCTTGCCCCGGTCCACCGACGTCCgg                                                                    >  1:4140321/1‑149 (MQ=255)
                                                                                    tCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAGTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCCCATCTTTCCCCGTGCCCCGGTCCCCCTCCGGCCGgtt                                                                  >  1:2083497/1‑150 (MQ=255)
                                                                                     ctaCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTa                                                                 <  1:5108850/148‑1 (MQ=255)
                                                                                          tGTCGAAATTCACCAGCCAAGGGCTGGCGAATTAACACCCGTGGGACTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCACAGATGTGGCAGACGCGGTCTGCCACATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTc                                                            <  2:3994454/150‑1 (MQ=255)
                                                                                            ttGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCg                                                          >  1:5243420/1‑150 (MQ=255)
                                                                                               aaaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTa                                                       >  1:3520919/1‑150 (MQ=255)
                                                                                                 aTTCACCAGACACTGTCTGGCGAATTAACCCCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                     <  2:4917226/150‑1 (MQ=255)
                                                                                                 aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                     <  1:3375248/150‑1 (MQ=255)
                                                                                                 aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                     <  1:2986195/150‑1 (MQ=255)
                                                                                                   tCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTTGCATTAGCTGTCTGGCAAATCTTTACCATTGCCCAGGTCCACCTAAGTCCGTTTTCTTTCCGTTaaaaa                                                   >  1:1007594/1‑150 (MQ=255)
                                                                                                      ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                >  1:3387827/1‑150 (MQ=255)
                                                                                                       cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCAACGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCt                                               >  2:2435138/1‑150 (MQ=255)
                                                                                                            aaTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGc                                          <  1:5339003/150‑1 (MQ=255)
                                                                                                              tGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                        <  1:4335334/150‑1 (MQ=255)
                                                                                                               gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCg                                       >  2:2641678/1‑150 (MQ=255)
                                                                                                               gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACCTTCCGTTAAAAACGCTGACGCCCg                                       >  2:2363737/1‑150 (MQ=255)
                                                                                                                  tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGTCGCTGTCTGCCAAATCTTTCCCCATGCCCAGGTCCACCGACGTCCGTTTACTTTCCGGTAAAAAAGCGGACGCCCGccg                                    >  1:1439486/1‑148 (MQ=255)
                                                                                                                    gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCt                                  >  2:2949514/1‑150 (MQ=255)
                                                                                                                    gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCt                                  >  2:4301869/1‑150 (MQ=255)
                                                                                                                    gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTACTTTCCGGTAAAAACGCTGACGCCCGCAGCt                                  >  2:4111914/1‑150 (MQ=255)
                                                                                                                      gAATTAACACCATTGGTAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCttt                                <  1:521000/150‑1 (MQ=255)
                                                                                                                      gAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGTCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTTCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCtgt                                >  2:5613409/1‑148 (MQ=255)
                                                                                                                       aaTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCtttt                               >  2:2266521/1‑150 (MQ=255)
                                                                                                                        aTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTa                              <  2:3869069/150‑1 (MQ=255)
                                                                                                                               ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                       <  1:1336735/150‑1 (MQ=255)
                                                                                                                               ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                       <  2:4806135/150‑1 (MQ=255)
                                                                                                                                cATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAag                      <  1:1111582/150‑1 (MQ=255)
                                                                                                                                 aTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga                     <  2:1909354/150‑1 (MQ=255)
                                                                                                                                 aTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga                     <  1:2486820/150‑1 (MQ=255)
                                                                                                                                  ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGGTAAAAACGCTGACGCCCGCAGCTTTGAGGAAAAagag                    >  2:2989126/1‑150 (MQ=255)
                                                                                                                                       aaTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCATGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAAGCCCGCAGCTTTTATGAAAAAGAGACGcg               >  1:3630634/1‑149 (MQ=255)
                                                                                                                                        aTTCTGCAGACGGTGTCTGCGGAATTCCCAATGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGGCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTc              >  2:5786520/1‑150 (MQ=255)
                                                                                                                                        aTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCATACGCTGTATTCCAAATCTTTCACCATGCCCAGGTACACCTACGTCCGTTTACTTTACGTTAAAAAACCTGAAGCCCGCAGCGTTTATGTAAAAGTGACGCTc              >  1:942413/1‑150 (MQ=255)
                                                                                                                                         ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTCTGCCCAATCTTTACCCTAGCCCAGGTCCACCGACGTCCGTTTAAATTCCGGTAAAAACGCGGACGCCCGCAGCTTTTATGAACAAGAGACGCTcc             >  1:1200529/1‑150 (MQ=255)
                                                                                                                                         ttCTGCAGACAGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc             >  2:2517640/1‑150 (MQ=255)
                                                                                                                                         ttCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc             >  2:3287969/1‑150 (MQ=255)
                                                                                                                                            cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt          <  1:480711/150‑1 (MQ=255)
                                                                                                                                                 acacCGACTGCATAATTCCCATCGGCAAAGATTTTGCAGACGCTGTCTGCCAAAGCTTTCCCCTTGCCCAGGTCCACCTACTTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCATCTTTTATGAAAAAGAGACGCTCCGCTGTGGc     <  1:4270827/150‑1 (MQ=255)
                                                                                                                                                  cacCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCt    <  2:787848/150‑1 (MQ=255)
                                                                                                                                                   acCGTCTGCAGAATTCCCATCGGCAATGATTTGGATTACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCTTTAAAATCGCTGACGCCCGCAGCTTTTATGAAAAGAGACGCTCCGCTGTGGCTgg  <  1:3880969/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGG  >  NC_000913/3368033‑3368330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: