Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,880 A→C 5.4% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8800AC 5.4% 856.9 / 2.8 282intergenic (+97/+55)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (106/150);  new base C (18/4);  total (125/157)
Fisher's exact test for biased strand distribution p-value = 2.66e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

TCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTG  >  NC_000913/3504731‑3505029
                                                                                                                                                     |                                                                                                                                                     
        cgcgCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCg                                                                                                                                               >  1:5747105/1‑150 (MQ=255)
        cgcgCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACTCCCCAAAGGGGCg                                                                                                                                               >  1:2441895/1‑150 (MQ=255)
        cgcgCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGCCCCTCACCCAAAACCTCTCCCCCAAGGGGCg                                                                                                                                               >  1:5481415/1‑150 (MQ=255)
         gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATACCGTACGTTGCCCGCACCCTAAACCAATAACCAAAGGGGCGa                                                                                                                                              >  1:5256115/1‑150 (MQ=38)
           gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCgggg                                                                                                                                            >  2:2987131/1‑150 (MQ=255)
            cccagcGCATCACCTTCCCTCTGGATAATAAAAGACCTTCCCCGGAGGCCGGATACGAGGCGCCAGCATCGCATCCGGCGCTGCTGGCGCGTTGAATTTTCCATCCCGGACGTGCCCCCCCCCCTCCCCCTCTCCCCCCCGGGGCGAggg                                                                                                                                           <  1:737344/145‑1 (MQ=255)
            ccAATCGCATCACCTGAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATACGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAggg                                                                                                                                           >  2:4379838/1‑150 (MQ=255)
               aTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAc                                                                                                                                        <  2:119997/150‑1 (MQ=255)
               aTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCAGTACGTTGACCACAACCAAAACACCAACCCAAAAGGGGGAGGGGAc                                                                                                                                        >  1:37798/1‑150 (MQ=37)
               aTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGAGGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAc                                                                                                                                        <  1:1039810/150‑1 (MQ=255)
                   cATCACCTGAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGGTCCCCCCACCCAAACCCGCACCCCCAACCGGCGAGGGGccccca                                                                                                                                    >  2:4537870/1‑147 (MQ=37)
                       aCCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCGAACCCGCACCCCAAAGGGGCGCGGGGACCGTTCact                                                                                                                                >  2:1352924/1‑148 (MQ=38)
                        ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                                               <  2:3627590/150‑1 (MQ=255)
                         cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                                              <  1:701064/150‑1 (MQ=255)
                          ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACg                                                                                                                             <  1:1132569/150‑1 (MQ=255)
                          ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                                             <  1:3169095/150‑1 (MQ=255)
                           tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACgg                                                                                                                            <  2:5733826/150‑1 (MQ=255)
                            aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCGCTCCCCCAAGGGGCGGGGGGACCGTTCAGTACGGa                                                                                                                           >  2:866145/1‑150 (MQ=255)
                            aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCCAAGGGGCGAGGGGACCGTTCAGTACCGa                                                                                                                           >  2:3967216/1‑150 (MQ=39)
                               tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCAGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                                                        >  1:5298186/1‑150 (MQ=255)
                                aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAttt                                                                                                                       <  2:1502095/150‑1 (MQ=255)
                                 ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCAAAACCCAAAAGGGCGAGGGGGCCGGTCAGTACGGaagata                                                                                                                      >  1:4684266/1‑146 (MQ=37)
                                 ttGATAATAAAAGACATTAACCGTAGGACTGATAAGATTCGCCAGCATCGCATCCGGCGATGCTGGAGCGTTGAATTTTAAATCCCGTACGTTACCCACACCAAAAACCACACCCCAAAGTGGCGAGGGGACCGTTAAGTAAGGAAtttt                                                                                                                      >  1:3747346/1‑149 (MQ=255)
                                    ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                                                   <  1:5545416/150‑1 (MQ=255)
                                    ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACACCCAAAACCACACCCCAAAGGGGCGAGGGGACCCTTCAGTACCGAATTTAGCt                                                                                                                   >  1:4369651/1‑150 (MQ=38)
                                        taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                                               <  1:3417732/150‑1 (MQ=255)
                                        taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                                               <  1:3468817/150‑1 (MQ=255)
                                        gaaaaGACATGCCCCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                                               <  1:4485039/149‑1 (MQ=255)
                                          aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACgg                                                                                                             <  2:4625744/150‑1 (MQ=255)
                                           aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGt                                                                                                            <  2:4195998/150‑1 (MQ=255)
                                            aGACATTCACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                                           <  1:5410727/150‑1 (MQ=255)
                                            aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                                           <  1:2977763/150‑1 (MQ=255)
                                            aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                                           <  2:4271452/150‑1 (MQ=255)
                                                aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCACACCCCAAAGGGGCGAGGGGACCCGTCAGTACGGAATTTAGCTTGACCGTGCTAt                                                                                                       >  2:4725925/1‑150 (MQ=39)
                                                   aaCCGTAGGCCGGATAAGATGCTCCAGCATCGCATCGGGCGTAGCTGGCGCGTTGAATTTTACATCCCGGACGTTGCCCTCACCATCACCCTCTCCCCACAGGGGCGAGGGGACCTTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                                    <  1:24344/150‑1 (MQ=255)
                                                   aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                                    <  1:89544/150‑1 (MQ=255)
                                                          ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACAACCCAAAGGGGCGACGGGACCCTTCAGTACGGAAATTAGCTTGACGGTGCTAACCAGCATTaa                                                                                             >  2:5140465/1‑150 (MQ=255)
                                                           gCCGGATAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                            <  2:4316907/150‑1 (MQ=255)
                                                           gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                            <  1:3061661/150‑1 (MQ=255)
                                                           gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                            <  1:1773961/150‑1 (MQ=255)
                                                           gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                            <  2:2128822/150‑1 (MQ=255)
                                                              ggATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                                         <  1:4761880/150‑1 (MQ=255)
                                                                 tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                                      <  2:4586467/150‑1 (MQ=255)
                                                                  aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGc                                                                                     <  1:652373/150‑1 (MQ=255)
                                                                  aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGc                                                                                     <  1:1751981/150‑1 (MQ=255)
                                                                        cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAct                                                                               <  2:5440108/150‑1 (MQ=255)
                                                                          ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATActct                                                                             <  1:1361412/150‑1 (MQ=255)
                                                                          ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAGTAAATACTGGCAATActct                                                                             >  2:3910102/1‑150 (MQ=255)
                                                                           ccgcatcgcctcCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGGTCCCCTCACCCGAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTCGCTTGCCGGTGCTATCCAGCATTAAATACTGGCAATACtctc                                                                            <  2:5262482/148‑1 (MQ=255)
                                                                           cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACtctc                                                                            <  2:5025839/150‑1 (MQ=255)
                                                                            aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                                            >  1:2418150/1‑85 (MQ=38)
                                                                             gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCACCCCCAAGGGGCGAGGGGACCCGTAAGTACGGAATTTAGCTTGACGGGGCGATCCAGCATTAAATACTGGCCAAACTCTCAc                                                                          >  2:3107120/1‑150 (MQ=255)
                                                                             gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCACACCCCCAAGGGGCGGGGGGACCCGTAAGTACCGACATTATCTTGACGGTGCCATCCAGCATTAAATACAGGCAATACACTAAc                                                                          >  2:2686126/1‑150 (MQ=255)
                                                                                     tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAGCCCTCTCCCCAACGGGGCGCGGGGACCGGTCAGGCCGGAATTTAGCGGGACGGGGCTATCCAGCCTTCAATACGGGCCATACTCTCACGCACAATc                                                                  >  2:3790184/1‑150 (MQ=255)
                                                                                       cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                                                <  1:5185852/150‑1 (MQ=255)
                                                                                        ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                                               <  2:4243794/150‑1 (MQ=255)
                                                                                        ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                                               <  2:5292262/150‑1 (MQ=255)
                                                                                          cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcag                                                             <  1:866145/150‑1 (MQ=255)
                                                                                              gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                                         <  2:4578055/150‑1 (MQ=255)
                                                                                                   cgcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtctc                                                    <  1:1241611/150‑1 (MQ=255)
                                                                                                    gcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGGGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTctct                                                   >  2:3790658/1‑150 (MQ=255)
                                                                                                      gTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTc                                                 <  1:1641946/150‑1 (MQ=255)
                                                                                                       ttGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACACCCCAAAGGGGCGAGGGGACCGGTCAGTACCGAAATTAGCTTGACGGTGCTATCCAGCAATAAAAAATGGCAATAATATAACCCAAAATCCCCAGCACCGTCtcatca                                                >  2:3203514/1‑150 (MQ=255)
                                                                                                       ttGAATTTTACATCCCGTACGTTCCCCACAACCTAACCCACTACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCATGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCACCACCGtgaattaa                                                >  2:2414967/1‑143 (MQ=255)
                                                                                                        tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                                               <  2:4104271/150‑1 (MQ=255)
                                                                                                         gAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAcc                                              <  1:1165060/150‑1 (MQ=255)
                                                                                                            ttttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCt                                           <  1:4872418/150‑1 (MQ=255)
                                                                                                                  aTCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt                                     <  1:3803624/150‑1 (MQ=255)
                                                                                                                     ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                                  <  1:109093/150‑1 (MQ=255)
                                                                                                                     ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                                  <  2:3045001/150‑1 (MQ=255)
                                                                                                                     ccGTACGTTCCCCTCACCCTAACCCTCTCCCAAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                                  >  1:3662025/1‑150 (MQ=255)
                                                                                                                       gTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt                                <  1:412073/150‑1 (MQ=255)
                                                                                                                          cGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTaa                             <  1:2739289/150‑1 (MQ=255)
                                                                                                                           gTTCACCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGATCAGAACGTAATTAAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCACACGCAAAAACCCCAGCACCGTCTCAACACCGCTGAGATTAGGGATAAt                            >  2:750468/1‑150 (MQ=25)
                                                                                                                            ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg                           >  2:3770015/1‑150 (MQ=255)
                                                                                                                            ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg                           >  2:4884714/1‑150 (MQ=255)
                                                                                                                             tCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGc                          <  2:2030789/150‑1 (MQ=255)
                                                                                                                                ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGACTGTAGCTTGACGGTGCTCTCCCGCATGCACTACTGGCAATACTCTCACGCCAAATCCGCAGCACCGTCTCGTCACCGCTGCGAGGCGGGGTAATGCGac                       >  2:536660/1‑149 (MQ=37)
                                                                                                                                 cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt                      >  2:286627/1‑150 (MQ=255)
                                                                                                                                 cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt                      >  2:1862266/1‑150 (MQ=255)
                                                                                                                                 cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt                      <  1:415375/150‑1 (MQ=255)
                                                                                                                                 cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt                      >  1:949972/1‑150 (MQ=255)
                                                                                                                                 cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAAAACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt                      >  2:248154/1‑150 (MQ=255)
                                                                                                                                  tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc                     >  2:3471872/1‑150 (MQ=255)
                                                                                                                                      ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac                 >  2:3825660/1‑150 (MQ=255)
                                                                                                                                      ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac                 >  2:3355886/1‑150 (MQ=255)
                                                                                                                                      ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGCATTAACCCGAATCGcag                 <  2:2351677/150‑2 (MQ=255)
                                                                                                                                        aaaCCCACTCCCCAAACCGGCGAGGGGACCGTTCAGTACGGAATATAGCTAGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACaa               <  2:5616166/149‑1 (MQ=255)
                                                                                                                                              tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAGTCGGGTTAATGCGAATCGCACAATGTTCt         >  1:2293123/1‑150 (MQ=255)
                                                                                                                                                tcCCCAAAGTGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGa       >  2:1760290/1‑150 (MQ=255)
                                                                                                                                                gccccACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAACATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGa       <  2:1958634/149‑1 (MQ=255)
                                                                                                                                                  cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGAtt     >  2:4415673/1‑150 (MQ=255)
                                                                                                                                                  cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGAtt     >  2:590729/1‑150 (MQ=255)
                                                                                                                                                   ccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                                                       >  1:4025808/1‑99 (MQ=255)
                                                                                                                                                   ccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtg    >  2:1703377/1‑150 (MQ=255)
                                                                                                                                                    cAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtgt   >  1:4903727/1‑150 (MQ=255)
                                                                                                                                                    cAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtgt   <  1:3538829/150‑1 (MQ=255)
                                                                                                                                                    cAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtgt   <  2:2592527/150‑1 (MQ=255)
                                                                                                                                                    cAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtgt   <  2:788450/150‑1 (MQ=255)
                                                                                                                                                     aaaGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtgtg  >  1:2661969/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTG  >  NC_000913/3504731‑3505029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: