Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,482 A→C 8.1% intergenic (+37/+50) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,4820AC 8.1% 724.6 / 8.4 275intergenic (+37/+50)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base A (76/167);  new base C (22/7);  total (101/174)
Fisher's exact test for biased strand distribution p-value = 5.46e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

ACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAA  >  NC_000913/3959333‑3959630
                                                                                                                                                     |                                                                                                                                                    
aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                                      <  2:2587799/150‑1 (MQ=255)
aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                                      <  1:1313445/150‑1 (MQ=255)
  gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGATCCTCTCCCACagg                                                                                                                                                    >  1:3793755/1‑150 (MQ=255)
  gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACagg                                                                                                                                                    >  1:3358557/1‑150 (MQ=255)
  gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTCCCACagg                                                                                                                                                    >  2:4363972/1‑150 (MQ=255)
    gCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACaggga                                                                                                                                                  <  1:5629692/150‑1 (MQ=255)
     cAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggag                                                                                                                                                 <  1:2109416/150‑1 (MQ=255)
      aaCTGCGTGATGGGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACcgggaga                                                                                                                                                <  1:59105/150‑1 (MQ=255)
        cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagg                                                                                                                                              <  1:2173007/150‑1 (MQ=255)
          gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                                            <  1:3370951/150‑1 (MQ=255)
             tGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGaa                                                                                                                                         <  1:3731397/150‑1 (MQ=255)
              gATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACCCCCCGACCCACTCCCACAGGGAGAGGGAGaaa                                                                                                                                        >  2:4772822/1‑150 (MQ=255)
                  tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCACTACCACCGGGAGAGGGAGAAAacac                                                                                                                                    >  1:5137175/1‑150 (MQ=255)
                  tgAACGAAGCGATTCGCAGCCATGCGAGTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGAAAGATATGAAACGTATTGCTGTTGCGGGTTAAGGGCGAGCTGATGCCCTCACCCCAACCCGCTCCCCCAGGGAGAGGGCCAAAacaa                                                                                                                                    >  2:707288/1‑149 (MQ=255)
                       gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAg                                                                                                                               <  1:1272155/150‑1 (MQ=255)
                         aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCACACCCCCACCCACACCCCCAGGGGGGGGGGGAACACACACAAGGc                                                                                                                             >  2:4073825/1‑150 (MQ=255)
                         aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                                             <  1:2816535/150‑1 (MQ=255)
                          gCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCACCCACACCCCCAGGGAGAGGGAGAAAACACACAACGcc                                                                                                                            >  1:1627979/1‑150 (MQ=255)
                           cGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagg                                                                                                                                                    >  2:428892/1‑125 (MQ=255)
                               tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATTCCCGCACCCCCACCCTCTACCACAGGGAGAGGGGGAAAACACACAAAGCCCtctc                                                                                                                       >  2:5447411/1‑150 (MQ=255)
                                 gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                     <  1:5348167/150‑1 (MQ=255)
                                 gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                     <  1:543538/150‑1 (MQ=255)
                                 gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                     <  2:1259032/150‑1 (MQ=255)
                                  cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                    <  1:2262868/150‑1 (MQ=255)
                                   aGCCATGCGATTGAGCAGGTAGGTAAGAATCTGTGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAGCACTCAAGGCCTTCTCCTgg                                                                                                                   <  2:5432589/150‑1 (MQ=255)
                                   aGCCATGCGATTGAGCAGGTAGGTAAGAAACTGTGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTgg                                                                                                                   <  2:2548338/150‑1 (MQ=255)
                                    gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                                  <  2:2385317/150‑1 (MQ=255)
                                    gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGACTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                                  <  2:5065286/150‑1 (MQ=255)
                                       aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAATCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                                               <  2:3913711/150‑1 (MQ=255)
                                       aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGTGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                                               <  2:4226606/150‑1 (MQ=255)
                                        tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCACCCACACCCACCCGGAGAGGGAGAAAAAACACAACGCCTACTCCGGGGGAAg                                                                                                              >  2:2639801/1‑150 (MQ=255)
                                           gATTGAGCAGGTAGGTAAGAATCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGcc                                                                                                           <  1:318404/150‑1 (MQ=255)
                                           gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGcc                                                                                                           <  2:3806010/150‑1 (MQ=255)
                                             ttGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACAAGGAGAGGGAGAAAAAAAACAAGGCCCACACCCGGAGAACGccgt                                                                                                         >  2:1671673/1‑148 (MQ=255)
                                                 gCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCACTCCCACAGGGAGTGGGAGAAAACACAAAAGGCCTTCTCCGGGAGAAGGCCGTGCtc                                                                                                     >  1:5247224/1‑149 (MQ=255)
                                                   aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt                                                                                                   <  1:1374507/150‑1 (MQ=255)
                                                   aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCGAACCCACACGGAGAGGGACAAAACACACAAGGCCGTATCCAGGAGAAGGCCTTGCGAtt                                                                                                   >  1:4368675/1‑150 (MQ=255)
                                                    ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                                                  <  1:2748934/150‑1 (MQ=255)
                                                     gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAg                                                                                                 <  1:4146049/150‑1 (MQ=255)
                                                         gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                                                             <  2:645067/150‑1 (MQ=255)
                                                          taAGAAACTGTGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                                                            <  2:5322228/150‑1 (MQ=255)
                                                          taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCGGGAGAAGGCCGTGCTATTAGTTGCg                                                                                            >  2:1398008/1‑150 (MQ=255)
                                                          taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTACCACAGGGAGAGGGGTGAAACACGCAAGGCCGTCACCTGGAGAAGGCCGTGCTATTAGTTGCg                                                                                            >  1:1933675/1‑150 (MQ=255)
                                                          taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGGTAAGTGCGCGCTGATGCCCGCACCCCCACCCACACCCACCCGGCGAGGGGGCAAAAACGCAAGGCCGTCTCCCGGGGAAGGCCGGGCGATTAGTTGCg                                                                                            >  1:825699/1‑150 (MQ=255)
                                                           aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                                           <  1:1889237/150‑1 (MQ=255)
                                                           aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGGCCACACCCCCACCCAAAACCACCCGGGGAGGGAGAAAACAAACAAGGCCGTATCCGGGAGAACGCCATGCAAATAGtttagg                                                                                           >  1:1588138/1‑146 (MQ=255)
                                                              aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCGATCCCCCACGGGGAGGGCGAAAAAACACAACGCCATGTGCGGGAGGAGGCCGTGGTATTAGTTTCCGTAc                                                                                        >  2:1059397/1‑150 (MQ=255)
                                                                  tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGTCCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCa                                                                                    <  2:1291448/150‑1 (MQ=255)
                                                                     gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACCGGGAGTGGGAGAAAACACGCAAGGCCTTCTCCGGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCt                                                                                 >  2:1467419/1‑150 (MQ=38)
                                                                             atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                         <  2:2094606/150‑1 (MQ=255)
                                                                             atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                         <  2:5726887/150‑1 (MQ=255)
                                                                              tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                        <  1:4708506/150‑1 (MQ=255)
                                                                              tGACAGATATGAAACGTATTGCTGTTGCGGGTTAACTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                        <  2:3142322/150‑1 (MQ=255)
                                                                               gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACACAATGCCTTCTCCGGGAGTAGGCCGTGCTATTAGTTGCGGGACCGCACCTTAatgatggg                                                                       >  2:455665/1‑148 (MQ=37)
                                                                                aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                      <  1:1093317/150‑1 (MQ=255)
                                                                                 cAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTGCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat                                                                     <  1:3453290/150‑1 (MQ=255)
                                                                                  aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCAAAATGATGTGata                                                                    >  2:5226326/1‑150 (MQ=255)
                                                                                    atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCACTCCCACCCGGGGATGGAGAAAAAAAACAACGCCATCTACAGGCGGAGGCCCTGCTATTAGTTGCGGTACAGCACCAAAATTATTTGataaa                                                                  >  2:1151170/1‑148 (MQ=255)
                                                                                    atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCCCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                  <  1:4296205/150‑1 (MQ=255)
                                                                                            cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                          <  2:4664762/150‑1 (MQ=255)
                                                                                             gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                                                         <  1:359307/150‑1 (MQ=255)
                                                                                              tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                        <  1:119928/150‑1 (MQ=255)
                                                                                               aTTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtg                                                       <  2:2918089/150‑1 (MQ=255)
                                                                                                ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                                                      <  1:3892943/150‑1 (MQ=255)
                                                                                                ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAAAACACAAGGCCTTCTCCTGGAGAAGGCCTTGCGATTAGTTGCGGTACAGAACCTTAATGATGTGATATCCGTACTgtgtgt                                                      >  2:3791278/1‑150 (MQ=38)
                                                                                                 tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCCAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg                                                     <  2:1207483/150‑1 (MQ=255)
                                                                                                    tGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAACCCGACCCACACCCACAGGGCGAGGGAGAAAACACTCAAGGCCGTCTCCGTGAGAAGGCCTTGCTATTAGTTGCGGTACCGCACCTTAATGATGTGATATCCGAACAGGGTGTGCAg                                                  >  2:3327288/1‑150 (MQ=255)
                                                                                                      ttGCGGGTTAAGTGCGCGCTGATGCCCACAACCCCACCCACACCCACACGGAGAGGGAGAAAAAACTAAAGGCCATATCCTGGAGAAGGCCATGCTATTAGTTGCGGTAAAGCACCGAAATTATTTGATATACCAACAGGGGGTGCAGCg                                                >  2:732515/1‑150 (MQ=255)
                                                                                                           ggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg                                           <  1:2325245/150‑1 (MQ=255)
                                                                                                           ggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg                                           <  1:2431836/150‑1 (MQ=255)
                                                                                                           ggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg                                           <  1:2496451/150‑1 (MQ=255)
                                                                                                             ttAAGTGCGCGCTGATGCCCTCCCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTGCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt                                         <  2:3472930/150‑1 (MQ=255)
                                                                                                             ttAAGTGCGCGCTGATGCCCGCACCCCCACCCACACCCACACGGAGAGGGAGAAAACACACAAGGCCTTAAACGGGAGAAGGCCGTGCGATTAGTTGCGGTACAGCACCGTAATGATGTGATAAACGAACAGGGTGTGCAGCGGGcccgg                                         >  2:192966/1‑147 (MQ=255)
                                                                                                              tAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                                        <  1:1480226/150‑1 (MQ=255)
                                                                                                                 gTGCGCGCTGATGCCCACACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTCAAGGCCTTCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGc                                     <  2:2075138/150‑1 (MQ=255)
                                                                                                                  tGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCt                                    <  2:4040593/150‑1 (MQ=255)
                                                                                                                   gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                   <  1:4745523/150‑1 (MQ=255)
                                                                                                                   gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                   <  2:4754926/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                 <  1:4932628/150‑1 (MQ=255)
                                                                                                                        cTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTa                              <  1:2924646/150‑1 (MQ=255)
                                                                                                                          gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                            <  1:4453089/150‑1 (MQ=255)
                                                                                                                           aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTCCAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg                           >  2:4174951/1‑150 (MQ=255)
                                                                                                                             gCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGa                         <  2:1785349/150‑1 (MQ=255)
                                                                                                                             gCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGa                         <  2:3273594/150‑1 (MQ=255)
                                                                                                                                cTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACaa                      <  1:5618123/150‑1 (MQ=255)
                                                                                                                                  cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                    <  1:341067/150‑1 (MQ=255)
                                                                                                                                  cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                    <  1:3299252/150‑1 (MQ=255)
                                                                                                                                   aCCCCGACCCTCTCCCACAGGGAGAGGGAGAAACCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAagag                   <  2:3038584/150‑1 (MQ=255)
                                                                                                                                     cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                 <  1:2581449/150‑1 (MQ=255)
                                                                                                                                     cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                 <  1:4230868/150‑1 (MQ=255)
                                                                                                                                       cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa               <  1:2996855/150‑1 (MQ=255)
                                                                                                                                       cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa               <  2:5528940/150‑1 (MQ=255)
                                                                                                                                       cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGGGATATCCGAACTGGGTGTGCAGCGGGCCGGGCGGCTCCAGTACCGGACCAGTGaaaa               >  2:1723900/1‑150 (MQ=255)
                                                                                                                                          cccTCGCCCTGAGGGAGAGGGAGAATACCCTCAAGGCCTTCTCCTGGAGAAGGCCTTGGTATTAGTTGCGGTACAGCACGTTTATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAATAcga            <  2:4866067/150‑3 (MQ=255)
                                                                                                                                               tcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTa       <  2:5011056/150‑1 (MQ=255)
                                                                                                                                               tcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTa       >  1:5703682/1‑150 (MQ=255)
                                                                                                                                                 ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc     >  2:5167353/1‑150 (MQ=255)
                                                                                                                                                   acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGa   >  1:4592781/1‑150 (MQ=255)
                                                                                                                                                    caGGGAGAGTGAGAAAACACTCATGGCCTTCTCGTGTAGATGGGCTTGCTATTAGTTGGGGTACAGCACCTTAATGATGTGATATCCTAAGTGGGTGTGCTGCGGGCCGGTCGGCTCCATTACGGGACAAGATAAAACCACTTTATCGaa  <  1:4070236/150‑1 (MQ=255)
                                                                                                                                                    caGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGaa  <  2:1856364/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAA  >  NC_000913/3959333‑3959630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: