BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,362,578	G→T	72.0%	noncoding (49/76 nt)	pheU ←	tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,362,578	0	G	T	72.0%	602.1 / inf	357	noncoding (49/76 nt)	pheU	tRNA‑Phe
Reads supporting (aligned to +/- strand): ref base G (57/43); new base T (129/127); total (187/170)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 359 total aligned reads displayed.

ATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGT  >  NC_000913/4362429‑4362727

    cTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGaca                                                                                                                                                   >  2:3093577/1‑150 (MQ=255)

     tGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAATCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGTACCAAGGacac                                                                                                                                                  >  2:4076994/1‑150 (MQ=255)

       gTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACgg                                                                                                                                                >  2:4298759/1‑150 (MQ=255)

             tgGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATAGTGGCGTCCTGAAACGAAAAACGGACATCCGTGGAGGTCCGTTTATATGAATTTTGTTCCCGGACTCGGAATCGAACCAATGACACGGGGAttt                                                                                                                                          >  2:1626845/1‑150 (MQ=255)

                ggAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCa                                                                                                                                       >  1:1124975/1‑150 (MQ=255)

                  agaATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAAt                                                                                                                                     <  1:5523182/150‑1 (MQ=255)

                     aTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATccc                                                                                                                                  <  1:1981984/150‑1 (MQ=255)

                        tGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTGAAAATccccgt                                                                                                                               <  1:3604638/150‑3 (MQ=255)

                         ggTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGc                                                                                                                              >  1:176834/1‑150 (MQ=255)

                           ttGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGctc                                                                                                                            >  2:275734/1‑150 (MQ=255)

                                ttCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCg                                                                                                                       >  1:4173645/1‑150 (MQ=255)

                                ttCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCg                                                                                                                       >  2:2358307/1‑150 (MQ=255)

                                ttCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCg                                                                                                                       >  1:3635588/1‑150 (MQ=255)

                                ttCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCg                                                                                                                       >  1:326434/1‑150 (MQ=255)

                                  cGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGAc                                                                                                                     <  1:5476748/150‑1 (MQ=255)

                                   ggTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACt                                                                                                                    <  1:4759859/150‑1 (MQ=255)

                                   ggTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACt                                                                                                                    >  2:5494221/1‑150 (MQ=255)

                                    gTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTg                                                                                                                   >  1:2332228/1‑150 (MQ=255)

                                    gTAGAAGTGATTTGGAGGCAGAACGCATAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTCGAGGGCCGTGTATAGGAAGTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCAGCTCTACCGCCTg                                                                                                                   >  2:5340383/1‑150 (MQ=25)

                                        aaTTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCt                                                                                                               >  2:4912944/1‑150 (MQ=255)

                                          ttGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTAt                                                                                                             >  2:3131538/1‑150 (MQ=255)

                                               ttGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCggg                                                                                                        <  2:4801200/150‑1 (MQ=255)

                                               ttGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCggg                                                                                                        <  2:1527556/150‑1 (MQ=255)

                                               ttGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCggg                                                                                                        <  1:1388239/150‑1 (MQ=255)

                                               ttGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCggg                                                                                                        >  2:5027517/1‑150 (MQ=255)

                                                 ggAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCa                                                                                                      >  1:3005165/1‑150 (MQ=255)

                                                  gAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCaa                                                                                                     >  1:2959411/1‑150 (MQ=255)

                                                  gAGGCAGAACGCTTAAATCGTGGCGACCTCAAACGAAAAACGGACCTCCGTGGAGGACCGTTTATATGTATTTGGTCCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCaa                                                                                                     >  2:5077310/1‑150 (MQ=25)

                                                   aGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAAc                                                                                                    >  2:3637047/1‑150 (MQ=255)

                                                   aGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAAc                                                                                                    >  2:843642/1‑150 (MQ=255)

                                                    ggCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACg                                                                                                   >  1:69308/1‑150 (MQ=255)

                                                         aaCGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGgcg                                                                                              <  1:130953/150‑1 (MQ=255)

                                                         aaCGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGgcg                                                                                              >  1:1410041/1‑150 (MQ=255)

                                                         aaCGCGTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGCGGTCCGTTTATATGACTTGGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGgct                                                                                              <  2:4703369/150‑2 (MQ=21)

                                                          aCGCTTACATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGACTTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGgcgc                                                                                             <  1:2063877/150‑1 (MQ=255)

                                                           cGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCa                                                                                            <  1:391405/150‑1 (MQ=255)

                                                            gCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCAt                                                                                           >  2:2863757/1‑150 (MQ=255)

                                                            gCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCAt                                                                                           <  1:2733600/150‑1 (MQ=255)

                                                              ttAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTa                                                                                         >  1:4899055/1‑150 (MQ=255)

                                                              ttAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTa                                                                                         >  1:4081909/1‑150 (MQ=255)

                                                              ttAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTa                                                                                         >  1:390639/1‑150 (MQ=255)

                                                               tAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGATTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTaa                                                                                        <  1:4508396/150‑1 (MQ=255)

                                                                aaaTCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTaaa                                                                                       <  2:2031993/150‑1 (MQ=255)

                                                                aaaTCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTaaa                                                                                       >  1:77291/1‑150 (MQ=255)

                                                                aaaTCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTaaa                                                                                       <  1:2863871/150‑1 (MQ=255)

                                                                   tCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTCCCGACTGAGCTATCCGGGCCACGGGGCGCATTAAACCg                                                                                    >  2:1385/1‑150 (MQ=255)

                                                                    cGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGt                                                                                   <  1:2194700/150‑1 (MQ=255)

                                                                     gTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTa                                                                                  <  2:5153199/150‑1 (MQ=255)

                                                                        gCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATc                                                                               <  1:1524911/150‑1 (MQ=255)

                                                                         cGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCt                                                                              <  1:322239/150‑1 (MQ=255)

                                                                            ccTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGca                                                                           <  1:5729683/150‑1 (MQ=255)

                                                                              tGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGACTCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTACTCTGcaca                                                                         >  2:526469/1‑150 (MQ=255)

                                                                              tGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGcaca                                                                         >  1:5237120/1‑150 (MQ=255)

                                                                              tGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCACTACCGACTGAGCTATCCGGGCAACGGGGCGCAATAAACCGTAATCTGcaca                                                                         >  2:3748137/1‑150 (MQ=255)

                                                                               gAAACGACAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCATGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAt                                                                        >  2:4410273/1‑150 (MQ=255)

                                                                               gAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAt                                                                        >  2:2190736/1‑150 (MQ=255)

                                                                                aaaCGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAtc                                                                       >  1:588594/1‑150 (MQ=255)

                                                                                 aaCGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCAGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAtct                                                                      >  1:4952198/1‑150 (MQ=255)

                                                                                  aCGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAtctc                                                                     >  2:1866211/1‑150 (MQ=255)

                                                                                     aaaaaCGGACCTCCGTGGAGGTCCGTGTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATGAACCCGTCCTCTGCACATCTCGTc                                                                  >  2:5785516/1‑150 (MQ=21)

                                                                                      aaaaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCa                                                                 <  1:442620/150‑1 (MQ=255)

                                                                                         aCGGACCTCCACGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAcc                                                              >  2:5392087/1‑150 (MQ=255)

                                                                                          cGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCt                                                             >  2:171167/1‑150 (MQ=255)

                                                                                            gACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTaa                                                           >  2:4754094/1‑150 (MQ=255)

                                                                                             aCCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAt                                                          <  1:5494221/150‑1 (MQ=255)

                                                                                              ccaccGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAtt                                                         <  2:2648395/147‑1 (MQ=255)

                                                                                               cTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAttt                                                        <  2:326434/150‑1 (MQ=255)

                                                                                               cTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAttt                                                        <  1:5027517/150‑1 (MQ=255)

                                                                                                tCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTc                                                       >  2:4595533/1‑150 (MQ=255)

                                                                                                tCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACTGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTc                                                       <  2:2178342/150‑1 (MQ=255)

                                                                                                tCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTc                                                       >  2:984232/1‑150 (MQ=255)

                                                                                                tCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTc                                                       >  1:4265633/1‑150 (MQ=255)

                                                                                                 ccGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCa                                                      <  1:1918972/150‑1 (MQ=255)

                                                                                                 ccGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCa                                                      <  1:4685795/150‑1 (MQ=255)

                                                                                                 ccGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCa                                                      <  2:2535714/150‑1 (MQ=255)

                                                                                                 ccGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCa                                                      >  1:988438/1‑150 (MQ=255)

                                                                                                   gTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGt                                                    <  1:5395494/150‑1 (MQ=255)

                                                                                                         gTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGc                                              >  2:3523830/1‑150 (MQ=255)

                                                                                                           ccGATTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGa                                            <  2:1291873/150‑1 (MQ=255)

                                                                                                              tttATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTc                                         >  1:944571/1‑150 (MQ=255)

                                                                                                                tataTGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCaa                                       <  2:1760696/150‑1 (MQ=255)

                                                                                                                 atatGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAAc                                      <  2:624850/150‑1 (MQ=255)

                                                                                                                 atatGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAAc                                      <  2:1085532/150‑1 (MQ=255)

                                                                                                                   atGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTg                                    >  1:1837771/1‑150 (MQ=255)

                                                                                                                    tGAATTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGc                                   >  2:3461709/1‑150 (MQ=255)

                                                                                                                      aaTTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCtt                                 >  2:5624490/1‑150 (MQ=255)

                                                                                                                      aaTTTGGTGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCtt                                 >  2:4124558/1‑150 (MQ=255)

                                                                                                                        tttGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTaa                               <  2:279801/150‑1 (MQ=255)

                                                                                                                             tGCCCGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTg                          <  2:784321/150‑1 (MQ=255)

                                                                                                                             tGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACAGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTg                          >  2:5092686/1‑150 (MQ=255)

                                                                                                                               cccGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCg                        >  2:3911216/1‑150 (MQ=255)

                                                                                                                                ccGGACTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCgg                       <  1:1904606/150‑1 (MQ=255)

                                                                                                                                ccGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCgg                       <  1:168012/150‑1 (MQ=255)

                                                                                                                                    aCTCGGAATCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCaaa                   <  2:1559844/150‑1 (MQ=255)

                                                                                                                                      tCGGACTCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATc                 <  2:2245004/150‑1 (MQ=255)

                                                                                                                                           aTCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGcttc            >  1:256248/1‑150 (MQ=255)

                                                                                                                                            tCGAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGcttct           >  2:2584159/1‑150 (MQ=255)

                                                                                                                                              gAACCAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCttt         >  1:3839039/1‑150 (MQ=255)

                                                                                                                                              gAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGcttctat         <  2:1692380/150‑3 (MQ=255)

                                                                                                                                                 ccAATGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCt      <  2:4534137/150‑1 (MQ=255)

ATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGT  >  NC_000913/4362429‑4362727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`