Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,506 C→G 6.6% intergenic (‑140/+43) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,5060CG 6.6% 998.8 / 8.5 302intergenic (‑140/+43)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (171/105);  new base G (2/23);  total (173/129)
Fisher's exact test for biased strand distribution p-value = 1.01e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

CATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCG  >  NC_000913/3908358‑3908655
                                                                                                                                                    |                                                                                                                                                     
 aTACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTg                                                                                                                                                     >  1:3770049/1‑150 (MQ=255)
  tACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                                                                                                                    <  2:3680060/150‑1 (MQ=255)
  tACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                                                                                                                    <  2:12954/150‑1 (MQ=255)
    cTCATTGCAATCTTTTCCTCATCTGGCCTGATGCACGTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgggg                                                                                                                                                  <  1:1445329/150‑1 (MQ=255)
     tCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                 >  1:397185/1‑150 (MQ=255)
     tCATTGCAATCTCTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTCGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCTAAATGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                 <  2:1017050/150‑1 (MQ=255)
        ttGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGcgag                                                                                                                                              >  2:1554783/1‑150 (MQ=255)
        ttGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                              <  1:1826335/150‑1 (MQ=255)
              tCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                                                                                        >  2:627179/1‑150 (MQ=255)
              tCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                                                                                        >  1:2379474/1‑150 (MQ=255)
                 tttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGt                                                                                                                                     <  2:4107117/150‑1 (MQ=255)
                    ccTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                                  >  2:4044498/1‑150 (MQ=255)
                    ccTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                                  <  1:2452158/150‑1 (MQ=255)
                      tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                                >  1:3000845/1‑150 (MQ=255)
                           gTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGAGCAGGCGTTCACGCCGCATCCGGCACAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaaa                                                                                                                           >  2:3250431/1‑150 (MQ=255)
                            tGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAc                                                                                                                          >  2:1846513/1‑150 (MQ=255)
                            tGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAc                                                                                                                          >  1:941037/1‑150 (MQ=255)
                            tGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAc                                                                                                                          >  2:2759835/1‑150 (MQ=255)
                             gCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAcc                                                                                                                         <  2:2618354/150‑1 (MQ=255)
                             gCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAcc                                                                                                                         >  1:3852470/1‑150 (MQ=255)
                                 gATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgtgt                                                                                                                     >  2:1200870/1‑150 (MQ=255)
                                   tGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCa                                                                                                                   >  1:2720205/1‑150 (MQ=255)
                                    gCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAg                                                                                                                  >  2:55858/1‑150 (MQ=255)
                                      aCTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCa                                                                                                                >  1:3383518/1‑150 (MQ=255)
                                       cTTCGCAAATAAGGCTTACCGTATGCCGGATCAGGCGTTCACCCCGCAACCGGCAAAGTGTTAAACCTGTACAGAGTAAAAAAGGCGCTCGGTCGGTCCCCAACCCCCACTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCaa                                                                                                               >  2:1664434/1‑150 (MQ=255)
                                       cTTCGCAAAACAGGCTTAACCTAGGCCGGATAAGGCGTTCAACCCGCATACCGCAAAGTGTTAAACCTGTAAAGAGTCAAAAAATCGCTAGGTCGGTCCCCACGCCCCACAGGGGAGAGGGTTAGGGTGAGGGGAAAAACCTTTCAGCaa                                                                                                               >  2:109246/1‑150 (MQ=19)
                                        ttCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAat                                                                                                              >  2:1506241/1‑150 (MQ=255)
                                        ttCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAat                                                                                                              >  2:2112913/1‑150 (MQ=255)
                                           gatAATCAGGCTTACGGTCGGCCGGATCTGGCTTTCACGCCGCATCCGGCATAGTTTTAACCGTGTACAGTGTAAAAACGGCGCTGGGTCGGCCCCCTCGCCCCTGGGGGTTGTGGTTTCGGGTGAGGGCAAAACCGTGTCAGCAATATc                                                                                                           <  2:3802481/148‑1 (MQ=255)
                                              aaTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGGTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                                                                                        >  1:110215/1‑150 (MQ=255)
                                                tCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCg                                                                                                      >  1:1981324/1‑150 (MQ=255)
                                                  aGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgtg                                                                                                    >  2:2868817/1‑150 (MQ=255)
                                                    gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGtt                                                                                                  <  2:2924212/150‑1 (MQ=255)
                                                    gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGtt                                                                                                  >  2:2724272/1‑150 (MQ=255)
                                                    gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGtt                                                                                                  <  2:1742074/150‑1 (MQ=255)
                                                    gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTGAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGtt                                                                                                  >  2:1771006/1‑150 (MQ=255)
                                                          cGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttct                                                                                            >  2:3277149/1‑150 (MQ=255)
                                                            tcggCCGTATCTGGCTTTCCCGCCGCAGCCGGAATTTTTTTACCGGTGTACAGAGTAAAAACGGGGCTGGGTGGGGCCCCTCGCCCCTGTGGGGTGTGGTTTTGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc                                                                                          <  1:3536189/148‑1 (MQ=255)
                                                            tcggCCGGATCAGGCGTTCACGCCGCATCCGGCAATGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc                                                                                          <  2:1138700/148‑1 (MQ=255)
                                                               gCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCa                                                                                       >  2:2242812/1‑150 (MQ=255)
                                                               gCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCa                                                                                       >  2:2305929/1‑150 (MQ=255)
                                                                ccGTATCTGGCGTCCCCCCCGCCCCCCGCAATTTTTTAACGGTGTACTCAGTCAAAACGGGGCTCGGGCGGCCCCCCCCCCCCGGTGGGTTGTGGGTTCGGGTGGGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaa                                                                                      <  1:3840719/150‑1 (MQ=255)
                                                                ccGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaa                                                                                      >  1:3506226/1‑150 (MQ=38)
                                                                 cGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaa                                                                                     >  1:2834301/1‑150 (MQ=255)
                                                                 cGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaa                                                                                     >  1:3919874/1‑150 (MQ=255)
                                                                  ggATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaaa                                                                                    >  1:441827/1‑150 (MQ=255)
                                                                       cggCGTCCCCGCCTCCCCCGCCAACGTGTTACCCGGGTACCGAGTCACAAAGGCGCGCGGGGGGGCCCCCCGCCCCCCTGGGGATAGGGTTCGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaac                                                                               <  1:512207/149‑1 (MQ=255)
                                                                         gCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgc                                                                             >  1:3694416/1‑150 (MQ=255)
                                                                         gCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAAGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgc                                                                             <  2:3871323/150‑1 (MQ=255)
                                                                             tCACGCCGCATCCGGCAATGTGTTAACCGTGTACAGAGTCAAAACGGCGCTCGGTGGGTCCCCTCGCCCCTCTGGGGAGTGGGTTTGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACgcgcgc                                                                         <  2:3889200/150‑1 (MQ=255)
                                                                             tCACGCCGCATCCGGAAAATTTTTAACGGTTTACAGCTTAAAAAAGGCGCTGGGTCGGTCCCCTGGCCCCTCTGGGGAGAGGTTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACgcgcgc                                                                         <  2:3856735/150‑1 (MQ=255)
                                                                               aCGCGGCATCGGGAAATGTGTTAACGGTGTACAGAGTCAAAAAGGCGCTGGGTGGGTCCCCTCGCCCCTCTGGGTTGAGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACTACGCGCGCCa                                                                       <  2:3169038/150‑1 (MQ=255)
                                                                               aCGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCa                                                                       >  2:3946940/1‑150 (MQ=255)
                                                                               aCGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGACCCCTCGCCCCACTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCa                                                                       >  2:310559/1‑150 (MQ=255)
                                                                                  ccgcATCCGGCAAAGTGTTAACCGTGTACAGAGGCACAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCGGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGGCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACACCGCGCGCCAGaa                                                                    >  2:649045/1‑150 (MQ=39)
                                                                                   cgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACACCGCGCGCCAGAAt                                                                   >  2:2918336/1‑150 (MQ=255)
                                                                                   cgcAGCCGGCAATGTGTTAACCGTGTACAGAGTAAAACTGGCGCTGGGTCGGTCCCCGCGCCCCTCTGGGGAGTGGGTTCGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAAt                                                                   <  1:2133425/150‑1 (MQ=255)
                                                                                     cATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGt                                                                 >  2:2352446/1‑150 (MQ=255)
                                                                                      aTCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCACAAACAACGCGCGCCAGAATGtt                                                                >  1:3608855/1‑150 (MQ=255)
                                                                                       tCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTc                                                               <  2:1391328/150‑1 (MQ=255)
                                                                                              atgtgtTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagcagca                                                        <  2:3961991/148‑1 (MQ=255)
                                                                                              aaGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagcagca                                                        >  2:1158025/1‑150 (MQ=255)
                                                                                              aaGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagcagca                                                        >  2:3217040/1‑150 (MQ=255)
                                                                                                 tgtTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTa                                                     >  1:2006785/1‑150 (MQ=255)
                                                                                                 tgtTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGGTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTa                                                     >  1:1911042/1‑150 (MQ=255)
                                                                                                     aaCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca                                                 >  2:2908981/1‑150 (MQ=255)
                                                                                                       cggtgtACAGAGTCAAACCGGCGCTCGGTCGGTCCCCGCGCCCCTGTGGGGTGAGGGTTTGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcaca                                               <  1:4044498/148‑1 (MQ=255)
                                                                                                       ccGTGTACAGCTAAAACACGGCGCTGGGTCGGTCCCCTCGCCCCTGTGGGGAGTGGTTTTGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcaca                                               <  1:1487708/150‑1 (MQ=255)
                                                                                                        ggtgtACAGAGAAAAAACGGCGCGGGGTGGGTCCCCGCGCCCCGGTGGGGTGGGGGTTGGGGTGGGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAg                                              <  1:1246120/149‑1 (MQ=255)
                                                                                                        cGTGTCCAGAGTCAAACTGGCGCTCGGTCGGCCCCCCCGCCCCTGTGGGTAGTGGTTTTGGGGGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAg                                              <  1:764362/150‑1 (MQ=255)
                                                                                                        cGTGTACATCGACAAACAGGCGCTCGGTCGGTCCCCTCGCCCCTGTGGGGAGTGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAg                                              <  1:2760559/150‑1 (MQ=255)
                                                                                                        cGTGTACAGTGTCAAAAAGGCGCTCGGTCGGGCCCCTCGCCCCTGTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAg                                              <  2:3355882/150‑1 (MQ=255)
                                                                                                         gtgtACAGAGACAAAATGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGTTTGTGGGTTTGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAgg                                             <  2:706633/150‑1 (MQ=255)
                                                                                                              cAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAAt                                        <  2:2444210/150‑1 (MQ=255)
                                                                                                              cAGAGAAAAAACGGCGCTCGGTCGGCCCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAAt                                        <  1:1544069/150‑1 (MQ=255)
                                                                                                               agagTCAAAACGGCGCTCGGTGGGTCCCCGCGCCCCTCTGGGGTGCGGGTTCGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATg                                       <  1:598351/150‑1 (MQ=255)
                                                                                                                   tCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCa                                   >  2:915445/1‑150 (MQ=255)
                                                                                                                     aaaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAAt                                 >  2:776497/1‑150 (MQ=255)
                                                                                                                        aaGGCGCTCGGTCGGCCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAcc                              <  1:3300627/150‑1 (MQ=255)
                                                                                                                          ggCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAcccc                            >  1:3642628/1‑150 (MQ=255)
                                                                                                                           gcgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCg                           >  2:2059757/1‑150 (MQ=255)
                                                                                                                            cgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCgg                          >  1:892832/1‑150 (MQ=255)
                                                                                                                             gcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGc                         >  2:2399448/1‑150 (MQ=255)
                                                                                                                             gcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGc                         >  2:3436489/1‑150 (MQ=255)
                                                                                                                             gcTCGGGCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGc                         <  2:1913660/150‑1 (MQ=255)
                                                                                                                              cTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGcc                        >  1:3164249/1‑150 (MQ=255)
                                                                                                                               tcggtcggTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGccc                       >  1:1396604/1‑150 (MQ=255)
                                                                                                                                cggtgggcCCCCTCGCCCCTGTGGGTAGTGGTTTTGGGTGGGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACACACAGGGTAATGATCAATACCCCGGCCCa                      <  2:1787082/150‑1 (MQ=255)
                                                                                                                                 ggtcggtcCCCTCGCCCCTGTGGGGTGTGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAg                     <  1:2210248/150‑1 (MQ=255)
                                                                                                                                  gtcggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAgg                    <  1:2402983/150‑1 (MQ=255)
                                                                                                                                   tcggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGc                   >  2:253346/1‑150 (MQ=255)
                                                                                                                                     ctaacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCa                 >  1:1055750/5‑150 (MQ=255)
                                                                                                                                           cgggCCCCTGGGGGGTGTGGGTTTGGGGGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  1:2868817/147‑1 (MQ=255)
                                                                                                                                           cgcGCCCCTCTGGGGAGTGGGTTAGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  2:3506550/148‑1 (MQ=255)
                                                                                                                                           cTCGCCCCTCTGGGGTGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  2:2967298/150‑1 (MQ=255)
                                                                                                                                           cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  1:2584211/150‑1 (MQ=255)
                                                                                                                                           cTCGCCCCGCTGGGGTGTGGGTTCGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  2:1458051/150‑1 (MQ=255)
                                                                                                                                            tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctg          <  1:1360881/150‑1 (MQ=255)
                                                                                                                                                   tctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTcc   >  2:2384188/1‑150 (MQ=255)
                                                                                                                                                   tctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTcc   >  2:852891/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCG  >  NC_000913/3908358‑3908655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: