Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 349,717 T→C 5.6% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913349,7170TC 5.6% 643.1 / 16.4 217intergenic (+145/‑295)prpB/prpC2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (54/142);  new base C (8/4);  total (71/146)
Fisher's exact test for biased strand distribution p-value = 7.36e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

ATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCG  >  NC_000913/349569‑349865
                                                                                                                                                    |                                                                                                                                                    
 tAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGa                                                                                                                                                    <  1:2122435/150‑1 (MQ=255)
  aaaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACGGAACTGTAGGTCGGATAAGAAGGTTGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGAc                                                                                                                                                   >  1:2680063/1‑150 (MQ=255)
  aaaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCGGACGCGCCGTATCAGGCCAACCAGGTGCACTGAACTGTAGGTCGGAAAAGACGGATGGCGTCGCAACCCACAACCCAAGCCAGAAGCGCCACTGACgagaa                                                                                                                                                   >  1:1491216/1‑149 (MQ=18)
  aaaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCAGATGCGTCGCTGACGCGACTTATAAGGCCAACGAGGTGCACGGCACTGTAGGTCGGATAAGACGGATGGCGTCGCCTCCGACAACCGAAGCCTGATGCGCCGCTGACGTGAc                                                                                                                                                   >  1:856199/1‑150 (MQ=255)
     aaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACAGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCCAAGCCTGATGCGCCGCTGACGCGACTTa                                                                                                                                                >  2:2919366/1‑150 (MQ=14)
      aaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTAt                                                                                                                                               <  1:3863203/150‑1 (MQ=255)
      aaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGGCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGAGACTTAt                                                                                                                                               >  2:1245552/1‑150 (MQ=255)
       aCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATc                                                                                                                                              <  2:1999541/150‑1 (MQ=255)
        cGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                                                                                                                             <  1:1221585/150‑1 (MQ=255)
        cGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                                                                                                                             <  1:1964008/150‑1 (MQ=255)
         gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCAGACGCGACTTATCAGGCCAACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAg                                                                                                                                            >  1:3318573/1‑150 (MQ=255)
          cccGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAgg                                                                                                                                           <  2:721520/150‑1 (MQ=255)
                gATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACg                                                                                                                                     <  1:1329311/150‑1 (MQ=255)
                gATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACg                                                                                                                                     <  1:2484588/150‑1 (MQ=255)
                  ttGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                                   <  1:3533673/150‑1 (MQ=255)
                    gTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGt                                                                                                                                 <  2:2479760/150‑1 (MQ=255)
                         cGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACt                                                                                                                            <  2:341107/150‑1 (MQ=255)
                            caaccGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGCGGTGCACTGAACTGTAGGTCGGATAAGACGGATTGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                                                         <  1:1475809/147‑1 (MQ=255)
                            cAGCCGATGCCTGCAGCGTCGCTGCCGCGACTTCTCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACACCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                                                         <  2:2334929/150‑1 (MQ=14)
                             aGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAAc                                                                                                                        >  2:1575042/1‑150 (MQ=255)
                                cGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGt                                                                                                                     >  2:1678460/1‑150 (MQ=255)
                                cGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGt                                                                                                                     <  2:2736945/150‑1 (MQ=255)
                                 gATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTa                                                                                                                    <  2:1801901/150‑1 (MQ=255)
                                 gATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTa                                                                                                                    <  1:2207013/150‑1 (MQ=255)
                                 gATGCCTGATGCGTAGCTGACGCGACTTATCAGGCCTACGAGTTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTTTACTGTa                                                                                                                    <  2:1605903/150‑1 (MQ=255)
                                      cTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg                                                                                                               <  2:3965250/150‑1 (MQ=255)
                                      cTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg                                                                                                               <  1:834152/150‑1 (MQ=255)
                                      cTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCGGACGTGACTTATCGGGCCTACGGGGTGAACTGAACTGTAGGGCg                                                                                                               >  2:410236/1‑150 (MQ=255)
                                       tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg                                                                                                              >  1:1007152/1‑150 (MQ=255)
                                       tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGAGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg                                                                                                              >  2:1211210/1‑150 (MQ=255)
                                        gATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                                             <  2:1546249/150‑1 (MQ=255)
                                           gCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATaa                                                                                                          <  1:749117/150‑1 (MQ=255)
                                            cGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAg                                                                                                         <  1:1723778/150‑1 (MQ=255)
                                             gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                                        <  2:2883334/150‑1 (MQ=255)
                                              tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGAc                                                                                                       <  2:76098/150‑1 (MQ=255)
                                                  tGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCAt                                                                                                   >  2:2461118/1‑150 (MQ=17)
                                                     cgcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGc                                                                                                <  2:2463232/150‑1 (MQ=255)
                                                      gcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACTGGACTTATCAGGCCTACGGGGAGCACAGAACTGAAGGTCGGATAAGACGCATAGCg                                                                                               >  2:1058263/1‑150 (MQ=11)
                                                        gACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                                             <  1:2614082/150‑1 (MQ=39)
                                                        gACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                                             >  1:3195454/1‑150 (MQ=39)
                                                          cTTATCAGTCCTACTAGGTGCACTGAACTGTAGGTCTGATAAGACGGATGGCGTCGCATCAGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAAATGTAGGTCGGATAAGACGCATAGCGTCGc                                                                                           <  1:3810009/150‑1 (MQ=14)
                                                             aTCAGGCCTACGAGGTGCACTGCACTGTAGGTCGGATAAGCCGGATGGCGTCGCCTCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                                                                        <  1:3263504/150‑1 (MQ=255)
                                                             aTCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGTCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                                                                        <  1:2854675/150‑1 (MQ=255)
                                                             aTCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                                                                        <  2:586061/150‑1 (MQ=39)
                                                             aTCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                                                                        <  1:3678470/150‑1 (MQ=39)
                                                             aTCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCACCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                                                                        <  1:182667/150‑1 (MQ=255)
                                                               cAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCg                                                                                      <  1:3193860/150‑1 (MQ=39)
                                                                aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                                                     >  1:1733092/1‑150 (MQ=39)
                                                                aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                                                     <  2:2575642/150‑1 (MQ=39)
                                                                   ccTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACaa                                                                                  <  1:2739116/150‑1 (MQ=39)
                                                                    cTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGAGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAc                                                                                 >  1:2364983/1‑150 (MQ=255)
                                                                     tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAcc                                                                                <  2:1385849/150‑1 (MQ=39)
                                                                       cGAGTTGCACTGAACTGTAGTTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGa                                                                              <  2:3789014/150‑1 (MQ=255)
                                                                       cGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGa                                                                              <  1:1656693/150‑1 (MQ=39)
                                                                       cGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGa                                                                              <  2:3789244/150‑1 (MQ=39)
                                                                        gAGGTGCACTGAACTGTCGGTCTGATAAGAGGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTTGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt                                                                             <  1:1824637/150‑1 (MQ=255)
                                                                         aGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATg                                                                            <  2:3094862/150‑1 (MQ=38)
                                                                            tGCACTGAACTGGAGGTCGGATAAGACGGTTGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCt                                                                         <  2:1763550/150‑1 (MQ=255)
                                                                                 tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgc                                                                    >  2:471370/1‑150 (MQ=37)
                                                                                   aaCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgcgc                                                                  >  1:3021660/1‑150 (MQ=37)
                                                                                    aCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgcc                                                                 <  2:2951853/150‑1 (MQ=37)
                                                                                     cTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccg                                                                <  1:1121250/150‑1 (MQ=37)
                                                                                           gTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCGGAcg                                                          >  2:2854957/1‑150 (MQ=37)
                                                                                           gTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACGTATCAGGCCTACGGGGTGCACTGAACCGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGAGGCGCCGCGGAcg                                                          >  2:3528494/1‑150 (MQ=255)
                                                                                             cGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgcg                                                        >  1:1024235/1‑150 (MQ=37)
                                                                                                aTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACt                                                     >  2:3216910/1‑150 (MQ=37)
                                                                                                    gACTGATGGCTTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTTAATTCTCAGGCCTACGTGGTGCACTGAACTGTAGGTCGGATAAGACGCATTGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATc                                                 <  2:2058629/150‑1 (MQ=11)
                                                                                                    gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATc                                                 <  2:2058540/150‑1 (MQ=37)
                                                                                                    gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATc                                                 <  1:2301976/150‑1 (MQ=37)
                                                                                                     aCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCa                                                <  2:1344177/150‑1 (MQ=37)
                                                                                                             cGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACg                                        <  2:4048733/150‑1 (MQ=32)
                                                                                                             cGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGCCCTACGGGGTGCACTGAACTGTAGCTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACg                                        <  2:434765/150‑1 (MQ=17)
                                                                                                              gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGACCTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACgg                                       <  2:3285525/150‑1 (MQ=17)
                                                                                                              gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACgg                                       >  1:620697/1‑150 (MQ=32)
                                                                                                              gTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAAGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACgg                                       >  1:620666/1‑150 (MQ=17)
                                                                                                               tCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACggg                                      <  2:3021660/150‑1 (MQ=32)
                                                                                                                  cATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTg                                   <  1:3917022/150‑1 (MQ=32)
                                                                                                                  cATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTg                                   >  1:917538/1‑150 (MQ=32)
                                                                                                                   aTCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGAt                                                                      >  2:3114640/1‑114 (MQ=32)
                                                                                                                   aTCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGAt                                                                      <  1:3114640/114‑1 (MQ=32)
                                                                                                                   aTCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGc                                  <  1:1511446/150‑1 (MQ=32)
                                                                                                                   aTCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGAATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGc                                  <  2:2815868/150‑1 (MQ=17)
                                                                                                                      cGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACt                               >  1:3336280/1‑150 (MQ=34)
                                                                                                                            ccGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTCTCAGGCCTACGGGGTGCACTGAACTg                         >  1:1471206/1‑150 (MQ=21)
                                                                                                                            ccGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGTTGCACTGAACTg                         >  1:2680467/1‑150 (MQ=21)
                                                                                                                             cGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGt                        >  2:2564952/1‑150 (MQ=34)
                                                                                                                                  ccTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc                   >  1:2943751/1‑150 (MQ=34)
                                                                                                                                  ccTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc                   >  1:3478227/1‑150 (MQ=34)
                                                                                                                                    tGATGCGCCGCTGACGTTACTTATCAGGACTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg                 <  1:928001/150‑1 (MQ=21)
                                                                                                                                    tGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg                 <  2:2178747/150‑1 (MQ=34)
                                                                                                                                     gATGCGCCGCTGTCGTTTCTTATCAGGCCTACGGTGTGCAGTTTACTGTAGTTCTGATAATACGCATTTCTTCGCATACGACATCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGTGGTGCACTGAACTGTAGGTCGGa                <  2:3660398/150‑1 (MQ=11)
                                                                                                                                     gATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTCCGGGGTGCACTGAACTGTAGGTCGGa                <  2:3023387/150‑1 (MQ=21)
                                                                                                                                        gcgcCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCATACGGGGTGCACTGAACTGTAGGTCGGATaa             <  2:1560619/150‑1 (MQ=21)
                                                                                                                                         cgccgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCAGAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAg            <  2:2143499/150‑1 (MQ=21)
                                                                                                                                          gccgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa           <  2:1615532/150‑1 (MQ=34)
                                                                                                                                          gccgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa           <  1:3622743/150‑1 (MQ=34)
                                                                                                                                                 aCGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGGGCACTGAACTGTAGGTCGGAGAAGACGCATAg    >  2:1876736/1‑150 (MQ=21)
                                                                                                                                                   gTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCg  >  1:1232115/1‑150 (MQ=33)
                                                                                                                                                   gTGACTTATCAGGCATACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCAAAGCGTCGCATCCGACAACCGATGCCAGAAGCGCCGCTGACGCGACTTATCAGTCCAACGGGGTGCACTGAACTGAAGGTCGGATAAGACGCATAGct  >  1:2536932/1‑149 (MQ=11)
                                                                                                                                                    tGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCTATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACggg                                      <  1:3950377/113‑1 (MQ=11)
                                                                                                                                                    |                                                                                                                                                    
ATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCG  >  NC_000913/349569‑349865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: