Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 12.4% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT12.4% 424.5 / 13.9 185intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (84/66);  new base T (19/9);  total (103/82)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177096‑2177394
                                                                                                                                                     |                                                                                                                                                        
 ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGa                                                                                                                                                         <  1:1450070/150‑1 (MQ=255)
  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                        >  1:537185/1‑150 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAat                                                                                                                                                      >  1:1624884/1‑150 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTGAAAATATAATGAAat                                                                                                                                                      >  2:1987512/1‑150 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                                      >  2:3046134/1‑150 (MQ=255)
      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAatta                                                                                                                                                    >  1:237919/1‑150 (MQ=255)
      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAatta                                                                                                                                                    >  1:2210996/1‑150 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                                  >  1:2693849/1‑150 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                                  >  1:1353266/1‑150 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATTAAattatt                                                                                                                                                  >  2:3935070/1‑150 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAATAAattatt                                                                                                                                                  >  1:1061282/1‑150 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAAATAtttt                                                                                                                                                >  1:2836351/1‑149 (MQ=255)
           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttttt                                                                                                                                               >  1:2722297/1‑148 (MQ=255)
           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                               <  2:4072468/150‑1 (MQ=255)
            cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                              <  2:3119588/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAAGAAATTATtttttt                                                                                                                                             >  1:3505920/1‑150 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                          <  2:2546656/150‑1 (MQ=255)
                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                         <  2:1817878/150‑1 (MQ=255)
                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                         <  2:3857547/150‑1 (MQ=255)
                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                          >  1:2415119/1‑149 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                    >  2:3580898/1‑150 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAATTAAAATATTTTTTTTTAAatata                                                                                                                                    >  1:1110647/1‑149 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                   <  1:1278701/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                  <  2:1582975/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                  <  1:2536531/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                  <  1:1332256/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                  <  2:101809/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                  <  1:2048880/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                  <  2:442847/150‑1 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATaa                                                                                                                             >  2:2426722/1‑150 (MQ=255)
                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTAATTGTTTTTAAATATCGAGATAACg                                                                                                                           >  2:3630077/1‑150 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATTTTATGAAATTATTTGTTTTTTTATATGGAGGTATCgt                                                                                                                          >  1:3047634/1‑149 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                          >  2:2705631/1‑150 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATaaacaa                                                                                                                         >  2:2449125/1‑146 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                                      <  2:1370266/150‑1 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                                      <  2:1370271/150‑1 (MQ=255)
                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACaa                                                                                                                    >  1:151967/1‑150 (MQ=255)
                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATTACGAtaaaaa                                                                                                                    >  1:1962541/1‑145 (MQ=255)
                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAATGAAATTTATTGTTTTTTAATATAGAGATAACGGTCaaaa                                                                                                                    >  1:3860484/1‑150 (MQ=255)
                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTGAATATCTCGATAACGATCACaa                                                                                                                    >  1:2242186/1‑150 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                 <  2:901450/150‑1 (MQ=255)
                                             ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTGAAAATATAATGAAATTATTTTTTTTTAAATATAGATATAAAGATAACAAAAAAGaa                                                                                                             >  1:1658295/1‑149 (MQ=255)
                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACa                                                                                                            >  2:3283470/1‑150 (MQ=255)
                                               ggACCCGCATAACCGCCGCGCTGTGCTTTGTTCAGCATCTTATTTGTCGATAACACTTTTTTTTTCATTTCCTTTCGTTTTTTTTCTTTTTAAATTTATATTTTAATTATTTTTTTTTAATTATCTTTATATGGTTAACAAAAACGActa                                                                                                           <  2:566955/150‑3 (MQ=255)
                                                gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAat                                                                                                          >  2:1981796/1‑150 (MQ=255)
                                                 aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAata                                                                                                         <  2:2864184/150‑1 (MQ=255)
                                                  aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTAAATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAatat                                                                                                        <  2:171856/150‑1 (MQ=255)
                                                    cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGCTTTAAAAATATAATGAAATTATTTGTTTTTATATATCGAGATAACGATCACCAAAACGACACTCTGa                                                                                                      >  1:4018697/1‑150 (MQ=255)
                                                      cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATAGAGATAAAGATAAAAAAAAAGACAataataaa                                                                                                    >  2:2123965/1‑145 (MQ=255)
                                                       aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTGAAAATATAATTAAAATATTTTTTTTTAAATATAGAGATAAAGAACACAAAAACGACAATATGaaaa                                                                                                   >  2:152840/1‑150 (MQ=255)
                                                       aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAAAAAAATGAAAATATTTTTTTTTTAATATAGGGCTAACGCTCACAAAAAACACCatatttaaa                                                                                                   >  1:841508/1‑145 (MQ=255)
                                                          aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTAGAATTCAAAATATAATTTAATTATTTTTTTTTAAAAATAGAGATAACGATCACAAAAACGAAAATATTAAAatta                                                                                                >  1:1995378/1‑150 (MQ=255)
                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATTAAAATATTTTTTTTTTAATATAGAGATAACAAAAACAAAAACGAAAAAATTAAAattattg                                                                                             >  1:2796046/1‑149 (MQ=255)
                                                               cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                           <  2:3232424/150‑1 (MQ=255)
                                                               cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                           <  2:1857423/150‑1 (MQ=255)
                                                                 cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagg                                                                                         <  2:369171/150‑1 (MQ=255)
                                                                  gcTGTGCGTTGTTCATCACCTTCTTTGTCTAAACCACTTACTTTTTACTATCCTGTCTTTTTTTTTCTATTAAAAAATATAATTAATTTTTTTGTTTTTAAATATCTAGATAACTAACAAAAAAACGACAATATGAAAATTATTCgagga                                                                                        <  1:154324/150‑1 (MQ=255)
                                                                  gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                                        <  2:3539662/150‑1 (MQ=255)
                                                                     gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGCGTg                                                                                    >  2:2598566/1‑150 (MQ=255)
                                                                          ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                               <  2:343658/150‑1 (MQ=255)
                                                                           tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAAATAAAATATTTTTTTTTAAATATAGAGATAACGATAAAAAAAAAAACAAAAAGAAAAATATTAGAGGCGTGtaaatc                                                                              >  1:2601350/1‑147 (MQ=255)
                                                                                aGCATCTGCTTTGTCGATACCACGTACATTTTCATAGCCTGTCGTTTTTTTTCTTTTTCAAAATATATTCAAATTTTTTTTTTTTAAATATCGATATAACGAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑aaaaa                                                                       <  1:3048072/150‑1 (MQ=255)
                                                                                 gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTTTAAAATAAAAATAAAATATTTTTTTTTTAATATAGTGGTAAACAAAAAAAAAAAAAAAAAATTAAAAATATTAGAAGTGTGTAATGC‑‑caaaaa                                                                      >  1:3909699/1‑150 (MQ=255)
                                                                                     cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATATTGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACgg                                                                  <  2:3617839/150‑1 (MQ=255)
                                                                                        cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGTTTTAAAAAAATAAATAAAATATTTTTTTTTTAATATCTATGTAAAGGTCACAAAAAAAACAATATGAAAAGAATTAGAGGAGATGAATGCC‑‑AAAAAAACgcca                                                                >  1:1485698/1‑147 (MQ=255)
                                                                                           tGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTccc                                                            <  2:2386540/150‑1 (MQ=255)
                                                                                             tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAAATATTCGAGGAGTGGAAGGC‑‑AAAAAAACGGCCTCCCGa                                                          >  2:1471281/1‑150 (MQ=255)
                                                                                             tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAATTATTTTTTTTTAAATATCGAGATAAAGATAACAAAAAAGACAAAATTAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAAAGGCCACCCGa                                                          >  2:1471614/1‑150 (MQ=255)
                                                                                               gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATa                                                        <  2:1851707/150‑1 (MQ=255)
                                                                                                         aCATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGt                                              >  2:2218460/1‑150 (MQ=255)
                                                                                                         aCATTTCAAAATCCTGTCTTTTTTTTTCTATTTCAAAATATAATCAATTTATTTTTTTTTAAATATCGAGATAACTATCAAAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCTTCCCTATCGGGAGGCCGt                                              <  1:3019826/150‑1 (MQ=255)
                                                                                                          ctttttCATATCCTGTCGTTTGTTTTCTTTTTAAAAATATAATCAATTTTTTTTTTTTTAATTATCGTCATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  1:1915259/148‑1 (MQ=255)
                                                                                                          ctttttCAGAGCCTGTCGTTTGTTTTCTTGTTAAAAATATACGGAATTTTTTTGTTTTAAAATATCGCGATAACCAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  1:2099412/148‑1 (MQ=255)
                                                                                                          cATTTTCATTTCGTTTTGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGATATAACTATCACAAAAACTACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  1:2517073/150‑1 (MQ=255)
                                                                                                          aaattacATATCCTTTCTTTTTATATCTATTACAAAATATAATGAAATTATTTTTTTTTAAATATCTAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  2:1840179/144‑1 (MQ=255)
                                                                                                                         tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                              >  2:1909021/1‑150 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATATTGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            <  2:2101847/150‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAATACGGCCTCCCGAGAGGGACGCCGTAGCAAAGTGCGCGTGttg                            >  2:3336281/1‑149 (MQ=255)
                                                                                                                            tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           >  1:769247/1‑150 (MQ=255)
                                                                                                                            tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGGGtttt                           >  2:3449625/1‑150 (MQ=255)
                                                                                                                             ttgtttTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          <  1:121843/150‑1 (MQ=255)
                                                                                                                                ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAATCGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:1992058/1‑150 (MQ=255)
                                                                                                                                ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:1992071/1‑150 (MQ=255)
                                                                                                                                ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:3632637/1‑150 (MQ=255)
                                                                                                                                ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  2:929721/1‑150 (MQ=255)
                                                                                                                                ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:4010166/1‑150 (MQ=255)
                                                                                                                                  ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:3101666/1‑150 (MQ=255)
                                                                                                                                  ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:4016793/1‑150 (MQ=255)
                                                                                                                                  ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:2192520/1‑150 (MQ=255)
                                                                                                                                   tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    >  1:1590648/1‑150 (MQ=255)
                                                                                                                                        ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  2:1652467/1‑150 (MQ=255)
                                                                                                                                        ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  2:2648605/1‑150 (MQ=255)
                                                                                                                                           aaaaTATAATGAATTTATTTGTTTTTAAATATCGTGATACCTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              <  2:698863/150‑1 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  2:1170853/1‑150 (MQ=255)
                                                                                                                                                ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACACACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGACCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       <  2:280147/150‑1 (MQ=255)
                                                                                                                                                ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       <  1:3768122/150‑1 (MQ=255)
                                                                                                                                                 taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      <  1:3248343/150‑1 (MQ=255)
                                                                                                                                                   aTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt    >  2:2022664/1‑150 (MQ=255)
                                                                                                                                                     gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAACGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  >  2:3039442/1‑150 (MQ=255)
                                                                                                                                                     gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  >  1:1324956/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177096‑2177394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: