Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,532 C→A 10.2% V69L (GTA→TTA)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5320CA10.2% 458.8 / 5.3 170V69L (GTA→TTA) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (87/58);  new base A (7/17);  total (95/75)
Fisher's exact test for biased strand distribution p-value = 7.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

TCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAAT  >  NC_000913/238386‑238680
                                                                                                                                                  |                                                                                                                                                    
tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                                                   >  2:168494/1‑150 (MQ=255)
  cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                                 >  1:280280/1‑150 (MQ=255)
  cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                                 <  1:2627977/150‑1 (MQ=255)
  cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGaa                                                                                                                                                 >  2:3712085/1‑150 (MQ=255)
      gACAAAAGGAGCTATTAAAGGTGCTATTAGGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCAGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAtt                                                                                                                                             >  2:203477/1‑150 (MQ=255)
        cAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTa                                                                                                                                           <  2:3912955/150‑1 (MQ=255)
            aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                       <  2:480233/150‑1 (MQ=255)
               aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                                                    >  2:3949193/1‑150 (MQ=255)
                gCTATTATAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTATCTGTATTTTTATGTCTGTGTCGAAATTGAGTACCTTCGTTTTTTTGTTTATGAATGATTTTATTATAGTGAGTAAGATTAGTTGAATATTTAATATGGaa                                                                                                                                   >  2:229664/1‑150 (MQ=255)
                  tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                 >  1:3701366/1‑150 (MQ=255)
                  tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                 >  2:2221672/1‑150 (MQ=255)
                   aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                                   <  2:3662963/147‑1 (MQ=255)
                     tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGa                                                                                                                              >  2:2785166/1‑150 (MQ=255)
                      aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                                             <  2:2211496/150‑1 (MQ=255)
                       aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTATGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                                            >  1:2289620/1‑150 (MQ=255)
                       aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                                            >  1:2022655/1‑150 (MQ=255)
                         ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATg                                                                                                                          >  2:1825854/1‑150 (MQ=255)
                         ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGACATg                                                                                                                          >  2:3562040/1‑150 (MQ=255)
                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATgt                                                                                                                         >  1:3513333/1‑149 (MQ=255)
                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                         >  2:1068825/1‑150 (MQ=255)
                            gCTATTACGATATATATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaaa                                                                                                                       <  2:2188643/150‑1 (MQ=255)
                             cTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAc                                                                                                                      >  1:854112/1‑150 (MQ=255)
                              tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                                     >  2:3990022/1‑150 (MQ=255)
                                        gCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGa                                                                                                           >  1:578489/1‑150 (MQ=255)
                                          tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                         >  2:1044995/1‑150 (MQ=255)
                                              attAAAAATATAATAGTTAGTTTTTTTTTTTTTTGTGTGGCTAATTTTACTACCTTCTTTTTTTTTATTAAGATTTTTTTTTTTATCTTAATTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                                     <  1:2324675/148‑1 (MQ=255)
                                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTTAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAAAt                                                                                                   >  1:4099244/1‑150 (MQ=255)
                                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAAATGAAAACGAATCACACAATCAACAt                                                                                                   >  1:2973343/1‑150 (MQ=255)
                                                 aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAATACATTAATATTTAAAAATGAAAAAGACATGAAACCGAAGCACAGAATCAACAtt                                                                                                  >  1:3724112/1‑150 (MQ=255)
                                                  aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                                 <  2:3051906/150‑1 (MQ=255)
                                                     atatAAGTTTTATCTTTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGATTTATTTTATTATCGTAAGTAAAATTAAATGAATATTAAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTcc                                                                                              <  1:620401/150‑1 (MQ=255)
                                                     atatAACAGTCACCTGTATTCTTATGTCTGTGGCCAAATTCACTACATACTATTTTTACATTAACAATCATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTcc                                                                                              <  2:3513333/150‑1 (MQ=255)
                                                       ataataGTTAGTTTTATTTTTCTGTCTGTGCCTAAATTCACAACATTCTTTTTTTTTATTAATAATTATTTTATTATCTTAATAAAATTTAAATCAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                            <  1:3479471/150‑1 (MQ=255)
                                                       ataAGATTTTGTTTTTTTGTTTTTTCTGTGGCTAAATTTAATACCTTCTTTTTTTTTATTAATATTGATTTTATTATCGTAATTAAAATTAAATGAATATTTAAAATGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                            <  1:3479511/150‑1 (MQ=255)
                                                       ataAGATTTTGGTTTATTGTTTTGTGTGTGGCTAAATTGAATACCTTCGTTTTTTTTATAAATAATGTTTTTATTATCTTAATTAAATTTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                            <  1:3027409/150‑1 (MQ=255)
                                                       ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAATCGACATGAAACCGAAGGACAGAATCAACATTCTCCaa                                                                                            >  2:1243290/1‑150 (MQ=255)
                                                              ttAGTTGTTTTTTTTTGTCTGTGGCTATTTTTAATACCTTGTTTTTTTTTTTTAAGATTTTTTTTTTTATGTTAATTAAAATTACATTAATTTTTAAAATTTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaa                                                                                     <  1:3137157/150‑1 (MQ=255)
                                                                aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                                   >  2:1519983/1‑150 (MQ=255)
                                                                 gcttttttGTTTTGTCTGTGGCGAATTTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTTTTATCGTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAata                                                                                  <  1:430170/144‑1 (MQ=255)
                                                                  cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                 >  2:1621000/1‑150 (MQ=255)
                                                                   tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTTATAATTATTTTATTATAGTAAGTAAAATTACATGAAAATTTAAAAATGAAAACGACATGAAACCGAATCACAGAATAAACATTCACCAAACAAAAAAAAtt                                                                                >  1:846018/1‑150 (MQ=255)
                                                                   tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAATACAAGAAAATTTAAAAAGGAAAACCACATGAAACCCAAGCACAGAAAAAACAATCACCAAACaaaaaaaaat                                                                                >  1:3747456/1‑146 (MQ=255)
                                                                         tttATGTCTGTGTCGAAATTTAATTCTTTCTTTTTTTTGATTTATATTGTTTTTATTATGTTAATTATTATTAAATGAATTTTTAAAATGGAAAACGACATGAAACCGAAGCACTGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                          <  2:1982748/149‑1 (MQ=255)
                                                                         gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                          <  2:1982760/150‑1 (MQ=255)
                                                                         gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAATTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAATCAACAATAATAAAATAATTACGTg                                                                          >  1:2282745/1‑150 (MQ=255)
                                                                          ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATTATTTTATTATAGTAAGTAAAAATAAAAGAATATTTAAAAAGGAAAAAGAAATGAAAACCAAACACAGAATAAAAAATATCCAAAAATAAAATATTTAAGTgg                                                                         >  1:3231171/1‑150 (MQ=255)
                                                                                atgtgGCTAAATTGAATACCTTCTTTTTTTTGATTAATAATGATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                   <  2:8189/149‑1 (MQ=255)
                                                                                    ggCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttat                                                               <  2:2317389/150‑1 (MQ=255)
                                                                                     gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                              >  2:3454211/1‑150 (MQ=255)
                                                                                     gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                              >  2:123112/1‑150 (MQ=255)
                                                                                     gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                              >  2:3454252/1‑150 (MQ=255)
                                                                                        aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                           <  2:1268498/150‑1 (MQ=255)
                                                                                        aaaTAGACTACCAAACTTTTTTTAATAAACAATCATTTTATTATCTAAATTAAAATTAAATCAAAATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                           <  2:766568/150‑1 (MQ=255)
                                                                                                 aCCTTCGTTTTTTTTATTAAGACTGATTGTTTTATCGTACGTAAATTTACATGAATATTTAAAACGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                                                  <  2:3112513/150‑1 (MQ=255)
                                                                                                  ccTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                 <  1:978202/150‑1 (MQ=255)
                                                                                                  ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                 <  2:614837/150‑1 (MQ=255)
                                                                                                  ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                 <  2:614838/150‑1 (MQ=255)
                                                                                                      cGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                             <  1:765477/150‑1 (MQ=255)
                                                                                                         ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                          <  1:1068825/150‑1 (MQ=255)
                                                                                                         ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                          <  1:176368/150‑1 (MQ=255)
                                                                                                         ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                          <  1:176378/150‑1 (MQ=255)
                                                                                                           ttttGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                        <  1:1533414/150‑1 (MQ=255)
                                                                                                           ttttGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                        <  2:266021/150‑1 (MQ=255)
                                                                                                           ttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                        <  1:2186894/150‑1 (MQ=255)
                                                                                                           ttttGATTAAGAATGATTTTATTATCGAAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCAGTGGAGCATTTTATTATTGAATATAGAGTTTTAACTc                                        >  2:3646098/1‑150 (MQ=255)
                                                                                                            ttttATTAAGAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                       <  2:3176585/150‑1 (MQ=255)
                                                                                                            tttGATTAATTATGATTTTATTATCGTATTTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                       <  2:2440757/150‑1 (MQ=255)
                                                                                                            tttGATTAATAATGATTTAATTATCGTAAGTAAAATTACATCAATATTTAAAAAGCAAAACGACATGAAACCGAACCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                       <  2:3503597/150‑1 (MQ=255)
                                                                                                            tttGATTAAGAATTATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                       <  2:2440754/150‑1 (MQ=255)
                                                                                                            tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                       <  1:3009645/150‑1 (MQ=255)
                                                                                                             ttGATTAAGAATTATTTTATTATCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                      <  2:134179/150‑1 (MQ=255)
                                                                                                             ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                      <  2:284947/150‑1 (MQ=255)
                                                                                                                aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                   >  1:2987742/1‑150 (MQ=255)
                                                                                                                 ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATCGAATATAGAGGTTTAACTCCGGTaa                                  >  2:1815010/1‑150 (MQ=255)
                                                                                                                  tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                 >  1:480192/1‑150 (MQ=255)
                                                                                                                  tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                 >  1:1563670/1‑150 (MQ=255)
                                                                                                                   aaGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                                >  1:1080503/1‑150 (MQ=255)
                                                                                                                   aaGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                                >  1:3591760/1‑150 (MQ=255)
                                                                                                                         ttttttATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                          <  2:2987742/148‑1 (MQ=255)
                                                                                                                         taTTTTATTATCGTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                          <  2:2987738/149‑1 (MQ=255)
                                                                                                                         gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                          <  2:431888/150‑1 (MQ=255)
                                                                                                                          aTTTTATTATCGTAAGTAAAATTACATGAATAATTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                         <  1:20983/150‑1 (MQ=255)
                                                                                                                           ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                        >  2:2421644/1‑150 (MQ=255)
                                                                                                                            tttATTATCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                       <  2:3805342/150‑1 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGACAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                       >  1:2436959/1‑150 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                       >  1:738362/1‑150 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                       >  1:2523130/1‑150 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                       >  2:2157431/1‑150 (MQ=255)
                                                                                                                                 tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                  <  2:3724129/150‑1 (MQ=255)
                                                                                                                                 tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                  <  2:1890554/150‑1 (MQ=255)
                                                                                                                                 taacGTAATTAAAATTACATGAATATATAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                  <  2:1594675/147‑1 (MQ=255)
                                                                                                                                    cGTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa               <  1:3152318/150‑1 (MQ=255)
                                                                                                                                     gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt              >  2:3163620/1‑150 (MQ=255)
                                                                                                                                        agtaaAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa           <  2:3231171/150‑1 (MQ=255)
                                                                                                                                        agtaaAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa           <  2:3468320/150‑1 (MQ=255)
                                                                                                                                            aaaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGa       >  1:1387004/1‑150 (MQ=255)
                                                                                                                                               ttACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa    >  2:2945563/1‑150 (MQ=255)
                                                                                                                                               ttACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa    >  1:1517818/1‑150 (MQ=255)
                                                                                                                                                tACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaaa   <  2:2973343/150‑1 (MQ=255)
                                                                                                                                                 aCATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaat  <  1:3077439/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAAT  >  NC_000913/238386‑238680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: