Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,535 G→T 7.7% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5350GT 7.7% 473.9 / 9.2 170H68N (CAT→AAT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (93/56);  new base T (2/16);  total (95/75)
Fisher's exact test for biased strand distribution p-value = 5.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATA  >  NC_000913/238386‑238684
                                                                                                                                                     |                                                                                                                                                     
tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                                                       >  2:168494/1‑150 (MQ=255)
  cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                                     >  1:280280/1‑150 (MQ=255)
      gACAAAAGGAGCTATTAAAGGTGCTATTAGGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCAGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAtt                                                                                                                                                 >  2:203477/1‑150 (MQ=255)
        cAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTa                                                                                                                                               <  2:3912955/150‑1 (MQ=255)
            aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  2:480233/150‑1 (MQ=255)
               aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                                                        >  2:3949658/1‑150 (MQ=255)
                gCTATTATAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTATCTGTATTTTTATGTCTGTGTCGAAATTGAGTACCTTCGTTTTTTTGTTTATGAATGATTTTATTATAGTGAGTAAGATTAGTTGAATATTTAATATGGaa                                                                                                                                       >  2:229664/1‑150 (MQ=255)
                gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                                       >  1:3126650/1‑150 (MQ=255)
                 cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGaaa                                                                                                                                      >  2:3240837/1‑150 (MQ=255)
                 cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaa                                                                                                                                      >  2:2420823/1‑150 (MQ=255)
                  tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                     >  2:2221672/1‑150 (MQ=255)
                  tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                     >  1:3468320/1‑150 (MQ=255)
                   aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                                       >  1:3662963/1‑147 (MQ=255)
                     tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGa                                                                                                                                  >  2:2785166/1‑150 (MQ=255)
                      aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                                                 <  2:2211496/150‑1 (MQ=255)
                         ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGACATg                                                                                                                              >  2:3562040/1‑150 (MQ=255)
                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATgt                                                                                                                             >  1:3513333/1‑149 (MQ=255)
                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                             >  2:1068825/1‑150 (MQ=255)
                             cTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACATGaaaa                                                                                                                          >  1:8189/1‑149 (MQ=255)
                             cTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAc                                                                                                                          >  1:854112/1‑150 (MQ=255)
                                      tAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                                                 <  2:1843069/150‑1 (MQ=255)
                                          tATTAGTAAAAATATAAGAGATAGCTGTATTGTTATGTCTGAGGCGAAATTGACTACCTTCGTTTTTTGGATTAAGAATGATTTTATAATCGTAAGTAAAATTACAGGAAAATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                             >  2:1044232/1‑150 (MQ=255)
                                           aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                            >  1:2296124/1‑150 (MQ=255)
                                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGAAATGAAACCGAAGCACAGAATCAACAt                                                                                                       >  1:2973342/1‑150 (MQ=255)
                                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTTAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAAAt                                                                                                       >  1:4099244/1‑150 (MQ=255)
                                                 aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAATACATTAATATTTAAAAATGAAAAAGACATGAAACCGAAGCACAGAATCAACAtt                                                                                                      >  1:3724112/1‑150 (MQ=255)
                                                     atatAAGTTTTATCTTTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGATTTATTTTATTATCGTAAGTAAAATTAAATGAATATTAAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTcc                                                                                                  <  1:620401/150‑1 (MQ=255)
                                                     atatAACAGTCACCTGTATTCTTATGTCTGTGGCCAAATTCACTACATACTATTTTTACATTAACAATCATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTcc                                                                                                  <  2:3513333/150‑1 (MQ=255)
                                                       ataAGATTTTGTTTTTTTGTTTTTTCTGTGGCTAAATTTAATACCTTCTTTTTTTTTATTAATATTGATTTTATTATCGTAATTAAAATTAAATGAATATTTAAAATGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                                <  1:3479511/150‑1 (MQ=255)
                                                       ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAATCGACATGAAACCGAAGGACAGAATCAACATTCTCCaa                                                                                                >  2:1243290/1‑150 (MQ=255)
                                                       ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATAGTAAGTAAAATTAAAAGAATATTTAAAAAGGAAAACGAAATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                                >  1:4018405/1‑150 (MQ=255)
                                                              ttAGCTGTTTTTTTATGTCTGTGGATAATTTGACTACATTGTTTTTTTTTTTTAATAATGATTTTTTTATCTTAATTAAAATTACATTAATTTTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaa                                                                                         <  1:53275/150‑1 (MQ=255)
                                                                aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                                       >  2:1519983/1‑150 (MQ=255)
                                                                   tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                                    >  2:1662947/1‑150 (MQ=255)
                                                                   tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAATACAAGAAAATTTAAAAAGGAAAACCACATGAAACCCAAGCACAGAAAAAACAATCACCAAACaaaaaaaaat                                                                                    >  1:3747456/1‑146 (MQ=255)
                                                                   tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCCAAGCACAGAATCAACAATCTCCAATCAAAAAATAtt                                                                                    >  1:3072743/1‑150 (MQ=255)
                                                                         gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                              <  2:1982760/150‑1 (MQ=255)
                                                                         gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGAATTTATTATAGTAAATAAAAATAAAAGAATAATTAAAAATGAAAACGACAAGAAAACCAAGCACACAATAAACAATAAACAATAATAAAAAATTTACGTg                                                                              >  1:2282750/1‑150 (MQ=255)
                                                                          ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATTATTTTATTATAGTAAGTAAAAATAAAAGAATATTTAAAAAGGAAAAAGAAATGAAAACCAAACACAGAATAAAAAATATCCAAAAATAAAATATTTAAGTgg                                                                             >  1:3231171/1‑150 (MQ=255)
                                                                                atgtgGCTAAATTGAATACCTTCTTTTTTTTGATTAATAATGATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                       <  2:8189/149‑1 (MQ=255)
                                                                                     gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                                  >  2:123112/1‑150 (MQ=255)
                                                                                     gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                                  >  2:3454252/1‑150 (MQ=255)
                                                                                      cGAAATTGACTACCTTCGTGTTTTTGTTTAAGAAAGATTTTATTATAGTTAGTAAAATTACAAGAAGAATTAAAAATGAAAACGACAAGAAACACCAGCACACAATCAACAATAACCAAAAATAAAAAATTTACGTGGAGCATGttatta                                                                 >  1:2997160/1‑150 (MQ=255)
                                                                                       gAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                                <  2:1974280/150‑1 (MQ=255)
                                                                                        aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                               <  2:1268498/150‑1 (MQ=255)
                                                                                        aaaTAGACTACCAAACTTTTTTTAATAAACAATCATTTTATTATCTAAATTAAAATTAAATCAAAATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                               <  2:766568/150‑1 (MQ=255)
                                                                                            tGACTACCTTCGTTTTTTTGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAat                                                           <  2:2975921/150‑1 (MQ=255)
                                                                                               cTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAAtata                                                        <  1:1538591/150‑1 (MQ=255)
                                                                                                  ccTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                     <  1:978202/150‑1 (MQ=255)
                                                                                                  ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                     <  2:614838/150‑1 (MQ=255)
                                                                                                  ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                     <  2:519036/150‑1 (MQ=255)
                                                                                                    ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                                   >  2:3111244/1‑150 (MQ=255)
                                                                                                     tCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGtt                                                  <  2:3184893/150‑1 (MQ=255)
                                                                                                         ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                              <  1:1068825/150‑1 (MQ=255)
                                                                                                         ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                              <  1:176368/150‑1 (MQ=255)
                                                                                                           ttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                            <  2:266023/150‑1 (MQ=255)
                                                                                                           ttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                            <  1:2186894/150‑1 (MQ=255)
                                                                                                           ttttGATTAAGAATGATTTTATTATCGAAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCAGTGGAGCATTTTATTATTGAATATAGAGTTTTAACTc                                            >  2:3646098/1‑150 (MQ=255)
                                                                                                            tttttttAATAATGATTTTATTATCGTATGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGATACCGAAGCACAGAATCAACATTCTCCAACCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                           <  2:2582702/145‑1 (MQ=255)
                                                                                                            ttttATTAAGAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                           <  2:3176585/150‑1 (MQ=255)
                                                                                                            tttGATTAAGAATTATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                           <  2:2440754/150‑1 (MQ=255)
                                                                                                            tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                           <  1:3009645/150‑1 (MQ=255)
                                                                                                             ttGATTAAGAATTATTTTATTATCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                          <  2:134179/150‑1 (MQ=255)
                                                                                                                aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                       >  1:2987738/1‑150 (MQ=255)
                                                                                                                aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                       >  1:2477900/1‑150 (MQ=255)
                                                                                                                 ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                      >  2:373323/1‑150 (MQ=255)
                                                                                                                 ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                      >  2:3488939/1‑150 (MQ=255)
                                                                                                                 ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATCGAATATAGAGGTTTAACTCCGGTaa                                      >  2:1815010/1‑150 (MQ=255)
                                                                                                                  tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                     >  1:1563670/1‑150 (MQ=255)
                                                                                                                  tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                     >  1:2096147/1‑150 (MQ=255)
                                                                                                                  tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                     >  1:480010/1‑150 (MQ=255)
                                                                                                                   aaGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                                    >  1:1080503/1‑150 (MQ=255)
                                                                                                                   aaGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                                    >  1:3591760/1‑150 (MQ=255)
                                                                                                                         taTTTTATTATCGTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              <  2:2987738/149‑1 (MQ=255)
                                                                                                                         gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              <  2:431888/150‑1 (MQ=255)
                                                                                                                          aTTTTATTATCGTAAGTAAAATTACATGAATAATTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                             <  1:20983/150‑1 (MQ=255)
                                                                                                                           ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                            >  2:2421644/1‑150 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGACAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                           >  1:2436959/1‑150 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                           >  1:738362/1‑150 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                           >  2:3023026/1‑150 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                           >  1:2523130/1‑150 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                           >  2:2157431/1‑150 (MQ=255)
                                                                                                                            tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                           >  2:2157453/1‑150 (MQ=255)
                                                                                                                                 tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                      <  2:3724112/150‑1 (MQ=255)
                                                                                                                                 tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                      <  2:1890554/150‑1 (MQ=255)
                                                                                                                                 taacGTAATTAAAATTACATGAATATATAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt                      <  2:1594675/147‑1 (MQ=255)
                                                                                                                                    cGTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa                   <  1:3152318/150‑1 (MQ=255)
                                                                                                                                     gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt                  >  2:3163620/1‑150 (MQ=255)
                                                                                                                                        agtaaAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa               <  2:3468320/150‑1 (MQ=255)
                                                                                                                                        agtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGTATTGAATGCa               >  2:3711201/1‑150 (MQ=255)
                                                                                                                                         gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              <  2:2854670/150‑1 (MQ=255)
                                                                                                                                           aaaaTTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAggg            <  2:3914621/150‑1 (MQ=255)
                                                                                                                                            aaaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGa           >  1:1387004/1‑150 (MQ=255)
                                                                                                                                              aTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaa         >  1:1925432/1‑150 (MQ=255)
                                                                                                                                               ttACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa        >  1:1517818/1‑150 (MQ=255)
                                                                                                                                               taaCATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa        >  1:765098/3‑150 (MQ=255)
                                                                                                                                                tACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaaa       <  2:2973342/150‑1 (MQ=255)
                                                                                                                                                    tGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataat   >  2:3633673/1‑150 (MQ=255)
                                                                                                                                                     gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAataata  >  1:2599949/1‑150 (MQ=255)
                                                                                                                                                     gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAataata  >  1:2771081/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATA  >  NC_000913/238386‑238684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: