Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 256,454 C→G 5.2% intergenic (+19/‑73) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913256,4540CG 5.2% 916.1 / 33.5 252intergenic (+19/‑73)gpt/frsAxanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base C (107/132);  new base G (6/7);  total (113/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 254 total aligned reads displayed.

TCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCC  >  NC_000913/256305‑256602
                                                                                                                                                     |                                                                                                                                                    
tCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCAc                                                                                                                                                      >  1:3855401/1‑150 (MQ=255)
 cACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACt                                                                                                                                                     >  2:710990/1‑150 (MQ=255)
  aCCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTg                                                                                                                                                    >  1:1565082/1‑150 (MQ=255)
   ccATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGc                                                                                                                                                   >  1:1600542/1‑150 (MQ=255)
    gaTCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACGATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATGTGGGCGACGTAGTCGTCCCGCCAATCTCCGGTCGCTATTCGTTTCCACGCCTGGCACTGcc                                                                                                                                                  >  1:755107/2‑150 (MQ=255)
     aTCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCg                                                                                                                                                 >  1:3329906/1‑150 (MQ=255)
     aTCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCg                                                                                                                                                 >  1:3354403/1‑150 (MQ=255)
        ttCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGc                                                                                                                                              >  2:3430886/1‑150 (MQ=255)
        ttCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGc                                                                                                                                              >  2:3600149/1‑150 (MQ=255)
         tCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCg                                                                                                                                             >  2:1566898/1‑150 (MQ=255)
          cGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgt                                                                                                                                            <  2:1329259/150‑1 (MQ=255)
          cGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGACTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgt                                                                                                                                            <  2:557499/150‑1 (MQ=255)
           gCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgtt                                                                                                                                           <  2:2470471/150‑1 (MQ=255)
           gCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgtt                                                                                                                                           <  1:702589/150‑1 (MQ=255)
           gCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgtt                                                                                                                                           >  2:2715962/1‑150 (MQ=255)
           gCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgtt                                                                                                                                           <  1:1485354/150‑1 (MQ=255)
           gCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgtt                                                                                                                                           <  2:1665112/150‑1 (MQ=255)
           gCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgtt                                                                                                                                           <  1:2469520/150‑1 (MQ=255)
                  cGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGTTATCCCGCATGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttt                                                                                                                                    <  2:2228800/150‑1 (MQ=255)
                  cGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttt                                                                                                                                    <  1:1304208/150‑1 (MQ=255)
                  cGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttt                                                                                                                                    <  1:3098410/150‑1 (MQ=255)
                   ggCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtttt                                                                                                                                   <  1:1073199/150‑1 (MQ=255)
                   ggCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtttt                                                                                                                                   >  2:3249280/1‑150 (MQ=255)
                   ggCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAAACTTTTCAACGCCTGGCACTGCCGGGCGGTGTTAtttt                                                                                                                                   >  1:1118494/1‑150 (MQ=255)
                   ggCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCGAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtttt                                                                                                                                   >  1:2173415/1‑150 (MQ=255)
                      tGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTaa                                                                                                                                <  2:3019725/150‑1 (MQ=255)
                       ggTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAc                                                                                                                               <  1:989010/150‑1 (MQ=255)
                         tcgtcCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACtt                                                                                                                             >  2:2725956/1‑150 (MQ=255)
                             ccGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCAGGCACTGCCGGGCGGTGTTCTTTTTAACTTCAgg                                                                                                                         >  2:3615014/1‑150 (MQ=255)
                                cTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCgg                                                                                                                      <  1:2899097/150‑1 (MQ=255)
                                cTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCgg                                                                                                                      <  2:455921/150‑1 (MQ=255)
                                 tGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCggg                                                                                                                     <  1:1671143/150‑1 (MQ=255)
                                 tGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCggg                                                                                                                     <  2:2763905/150‑1 (MQ=255)
                                       atgaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACaa                                                                                                               <  1:2295924/150‑1 (MQ=255)
                                         gaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATa                                                                                                             <  2:1133768/150‑1 (MQ=255)
                                         gaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATa                                                                                                             <  1:805788/150‑1 (MQ=255)
                                         gaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAAAGCCTGGCACTGCCGGGCGTTGTTATTTTTAAATTCAGGCGGGTTAAAATa                                                                                                             >  2:1193835/1‑150 (MQ=255)
                                              tgttgtTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTc                                                                                                        <  2:3549219/150‑1 (MQ=255)
                                                ttgttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCa                                                                                                      <  1:2816650/150‑1 (MQ=255)
                                                   ttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagta                                                                                                   >  2:3181825/1‑150 (MQ=255)
                                                   ttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGGTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagta                                                                                                   >  2:3906826/1‑150 (MQ=255)
                                                   ttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTAAACGCCAGGCACTGCCGGGCGTTGTTAATTTTAAAATAATGCGGGTTACAATAGTTTACagta                                                                                                   >  2:833970/1‑150 (MQ=255)
                                                    tgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtac                                                                                                  >  2:3551489/1‑149 (MQ=255)
                                                      atatCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTTATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCATGCGGGTTACAATAGTTTGaagtaagt                                                                                                >  1:3935787/1‑150 (MQ=255)
                                                      atatCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCAGGCCCAGCCGGGCGTTGTTGTTTTTAACATCAAGCCGGTTACAAAAGTTTCaagtaagt                                                                                                >  1:424440/1‑150 (MQ=255)
                                                         tCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTAtt                                                                                             >  2:3062129/1‑150 (MQ=255)
                                                         tCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCGTTTCAACGCCAGGCACTGCCGGGCGTTGTTCTTTTTAAATTCAGGCGGGTTACAAAAGTTTGCAGTAAGTAtt                                                                                             >  1:1903222/1‑150 (MQ=255)
                                                           ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCt                                                                                           <  2:3286733/150‑1 (MQ=255)
                                                           ccGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCt                                                                                           <  2:2595509/150‑1 (MQ=255)
                                                              cAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGa                                                                                        <  2:3845538/150‑1 (MQ=255)
                                                                aGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAATTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAgg                                                                                      >  1:3760729/1‑150 (MQ=255)
                                                                 gATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGc                                                                                     <  2:2030214/150‑1 (MQ=255)
                                                                      cTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCCACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTAAAATAGTTTCCAGGAAGTATTCTGGAGGCTGCag                                                                                >  2:1846489/1‑149 (MQ=255)
                                                                       tGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCGAATCTTTTCAACGCCTGGCACGGCCGGGCGGTGTTCTTTTTAAAATAACGCGGGTTAAAATAGTTTACAGTAAGTATTATGGAGGGGGCATc                                                                               >  1:1623166/1‑150 (MQ=255)
                                                                          aTTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCAt                                                                            <  2:2349251/150‑1 (MQ=255)
                                                                          aTTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCAt                                                                            <  2:1462913/150‑1 (MQ=255)
                                                                          aTTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCAt                                                                            <  1:2349522/150‑1 (MQ=255)
                                                                          aTTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCAt                                                                            <  1:1129937/150‑1 (MQ=255)
                                                                            tGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGa                                                                          <  1:2968699/150‑1 (MQ=255)
                                                                                 aGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGGTGTTCTTTTTAAATTCAGTCGGGTTACAATAGTTTACCGTAAGTATTCTGGGGGCTGCATCCAAGACACAg                                                                     >  1:2018888/1‑150 (MQ=255)
                                                                                  gCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAgg                                                                    <  2:1591094/150‑1 (MQ=255)
                                                                                                 cgtcgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGaaa                                                     <  2:3474650/150‑1 (MQ=255)
                                                                                                   tcgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAAcc                                                   <  2:1970185/150‑1 (MQ=255)
                                                                                                     gtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCt                                                 <  1:3785986/150‑1 (MQ=255)
                                                                                                        ttCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGCGGCTGCATCCAAGACACAGGCAAACCTGAGCGAAACCCTGtt                                              >  2:514555/1‑150 (MQ=255)
                                                                                                        ttCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGtt                                              <  2:1201305/150‑1 (MQ=255)
                                                                                                        ttCGTCCCGCCAATCTCCGGTCGCTAATCTTTACAACGCCTGGCACTGCCGGGCGTTGTACTTTATAACTTCACGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGtt                                              <  2:2843078/150‑1 (MQ=255)
                                                                                                        ttCGTCCCGCCAATCTCCGGTCGCTAATCGTTTCAACGCCTGGCACTGCCGGGCGGTGTTCTTTTTAACTTCAGGCGGGTTACAATAGGTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTgat                                              >  2:654824/1‑148 (MQ=255)
                                                                                                        gtCGTCCCGCCAATCTCCGGGCGCTAATCTTTTCAACGCCTGGCACTGCGGGGCGTTGTTCTTTTTTACTTCAGGCGGGTTACAATAGTGTCCTGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGtt                                              <  2:777742/149‑1 (MQ=255)
                                                                                                         tCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGGAAGGATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTc                                             >  2:2606132/1‑150 (MQ=255)
                                                                                                           gTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCaa                                           >  2:2858883/1‑150 (MQ=255)
                                                                                                            tcacGCCAATCTCCGGTCGCTAATATTTTCAACGCCTGGCACTTCCCGGCGTTGTTCATATTAAAATCCGGCGGGATACAAAAGTTAACAGTCAGTATTCTTGAGGCTGCATCCAATACACAGGCAAAAAAGAGCGAAACCCACTTCaaa                                          >  2:1634825/4‑150 (MQ=255)
                                                                                                              ccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAAcc                                        <  2:205762/150‑1 (MQ=255)
                                                                                                               cGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAAccc                                       <  2:1586673/150‑1 (MQ=255)
                                                                                                                gCCAATCTCCGGTCGCTAATCTTTTCAAAGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAAcccc                                      >  2:938545/1‑150 (MQ=255)
                                                                                                                 ccAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCg                                     <  1:3567420/150‑1 (MQ=255)
                                                                                                                 ccAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCg                                     <  1:865636/150‑1 (MQ=255)
                                                                                                                      ctcCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTa                                <  1:4100877/150‑1 (MQ=255)
                                                                                                                      ctcCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTa                                <  2:3407201/150‑1 (MQ=255)
                                                                                                                        cccctCGCTAAACTTTACAACGCCACGCACTGCCGCGCGTAGTTCATTATAACTACAGGCGGGTTACAATAGATTCCAGTAACTATACAGGAGGCAGCATCCATGACACACGCAAACCAGAGCGAAACCCTGTTCAAACCCCGCTTTaaa                              <  2:3025610/146‑1 (MQ=255)
                                                                                                                           gTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAATCCCTGTTCAAACCCCGCTTTAAACAt                           <  2:343538/150‑1 (MQ=255)
                                                                                                                           gTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACAt                           <  1:2725842/150‑1 (MQ=255)
                                                                                                                            tCGCGAATCTGTTCAACGCCGGGCACTGCCGGGCGTTGTTCTTTGTAACGTCAGGCGGGTTACAATAGTTTCCAGTCAGTATTCTGGAGGCTGCATCCATGACCCAGGCAAACCTGATCGAAACCCTGTTCAAACCCCGCTTTAAACATc                          <  2:3127735/150‑1 (MQ=255)
                                                                                                                             cGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATcc                         <  1:1243621/150‑1 (MQ=255)
                                                                                                                              gCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCt                        <  2:2210956/150‑1 (MQ=255)
                                                                                                                              gCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCt                        >  2:2564796/1‑150 (MQ=255)
                                                                                                                                tAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGa                      >  2:2292724/1‑150 (MQ=255)
                                                                                                                                      tttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTAATGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCAGTTCAAACACCGCTTTAAACATACTGaaaacac                >  1:2250791/1‑146 (MQ=255)
                                                                                                                                      tttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAATACCGGCGGGTTACAATAGTTTCCAGTTAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTc                <  1:3464857/150‑1 (MQ=255)
                                                                                                                                       ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCa                                                                                                      <  1:879818/63‑1 (MQ=255)
                                                                                                                                       ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCa                                                                                                      >  2:879818/1‑63 (MQ=255)
                                                                                                                                       ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGGTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCg               >  2:3700785/1‑150 (MQ=255)
                                                                                                                                       ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAAATAAGGCGGGTTACAATATTTTCCAGTAAGTATTCTGGAGGCTGCATCCAAGACACAGGCAAACCTGAGCGAAACCCAGTTCAAACCCCCCATTAAACATCCAGAAACCACg               >  1:2113026/1‑150 (MQ=255)
                                                                                                                                       ttCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAAATAAGGCGGGTTACAATATTTTACAGTAAGTATTATGGAGGCTGCATCCATGACACAGGCAAACCAGAGCGAAAACCAGTTCAAACCCCCCGTTAAAAATACAGAAACCTCg               >  1:2298082/1‑150 (MQ=255)
                                                                                                                                          ctcGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACg            <  1:2238352/148‑1 (MQ=255)
                                                                                                                                           aCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGc           >  1:604577/1‑150 (MQ=255)
                                                                                                                                             gCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTa         <  2:1118494/150‑1 (MQ=255)
                                                                                                                                                 ggCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTcc     <  1:1290367/150‑1 (MQ=255)
                                                                                                                                                 ggCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTcc     <  1:535184/150‑1 (MQ=255)
                                                                                                                                                    aCTGCCGGGCGTTGTTATTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCCGAAACCTCGACGCTAGTccgcc  <  2:1216147/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTCCGCC  >  NC_000913/256305‑256602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: