Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,437,445 G→A 27.3% A310T (GCC→ACC)  trkA → NAD‑binding component of TrK potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,4450GA27.3% 390.5 / 166.3 220A310T (GCC→ACC) trkANAD‑binding component of TrK potassium transporter
Reads supporting (aligned to +/- strand):  ref base G (94/66);  new base A (39/21);  total (133/87)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

Only 100 of 223 total aligned reads displayed.

TCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACTATTTCTGCGT  >  NC_000913/3437296‑3437589
                                                                                                                                                     |                                                                                                                                                
tCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAgg                                                                                                                                                  <  2:642467/150‑1 (MQ=255)
tCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAgg                                                                                                                                                  <  2:1734699/150‑1 (MQ=255)
 cGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGc                                                                                                                                                 >  2:1777968/1‑150 (MQ=255)
    aCGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCaa                                                                                                                                              >  1:929795/1‑150 (MQ=255)
    aCGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCaa                                                                                                                                              >  2:778660/1‑150 (MQ=255)
     cGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAat                                                                                                                                             <  1:4119244/150‑1 (MQ=255)
       tAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAatat                                                                                                                                           >  2:634893/1‑150 (MQ=255)
       tAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAatat                                                                                                                                           >  2:3889544/1‑150 (MQ=255)
         ttcggcagcGCGCTGCTGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCa                                                                                                                                         >  1:474299/5‑150 (MQ=255)
         ttcggcagcGCGCTGCTGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCa                                                                                                                                         >  1:647785/5‑150 (MQ=255)
            agcagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTGTCTTGCCGTCACCAACGATGACGAGACCAATATCCTGt                                                                                                                                      >  2:3984146/1‑150 (MQ=255)
              cagcGCGCTGCCGAACTGGCGGAAAAGTTTCAGAAGACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTcc                                                                                                                                    <  2:883108/150‑1 (MQ=255)
              cagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTcc                                                                                                                                    <  2:2438343/150‑1 (MQ=255)
                gcgcgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGc                                                                                                                                  <  2:3311196/150‑1 (MQ=255)
                      tGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTTCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCt                                                                                                                            >  2:175009/1‑150 (MQ=255)
                      tGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCt                                                                                                                            >  2:2364042/1‑150 (MQ=255)
                      tGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCt                                                                                                                            >  2:2347607/1‑150 (MQ=255)
                       gCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCtt                                                                                                                           <  1:3926194/150‑1 (MQ=255)
                        ctgAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTg                                                                                                                          <  2:474299/148‑1 (MQ=255)
                        ccGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTg                                                                                                                          >  1:2280567/1‑150 (MQ=255)
                          gAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGcc                                                                                                                        <  2:1224247/150‑1 (MQ=255)
                              tcgCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAAc                                                                                                                    <  2:3801779/148‑1 (MQ=255)
                              tGGCGGAAAAGTTACAGAATACGATCGTATTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAATAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAAc                                                                                                                    <  1:2561384/150‑1 (MQ=255)
                                     aaaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATggg                                                                                                             <  2:2793247/150‑1 (MQ=255)
                                      aaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGt                                                                                                            <  1:57268/150‑1 (MQ=255)
                                      aaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGt                                                                                                            <  1:3393048/150‑1 (MQ=255)
                                        gTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGc                                                                                                          <  1:1982213/150‑1 (MQ=255)
                                              gAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGaaaaa                                                                                                    >  2:2509340/1‑150 (MQ=255)
                                              gAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGACATGCTTGCCAAACGTATGGGTGCGaaaaa                                                                                                    <  1:1717464/150‑1 (MQ=255)
                                               aaTACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAg                                                                                                   >  1:2453993/1‑150 (MQ=255)
                                                 tACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGt                                                                                                 >  2:3063095/1‑150 (MQ=255)
                                                      tCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATgg                                                                                            <  2:2115760/150‑1 (MQ=255)
                                                       cGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGt                                                                                           <  1:3153215/150‑1 (MQ=255)
                                                        gTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTTCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTa                                                                                          >  1:1747296/1‑150 (MQ=255)
                                                        gTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTa                                                                                          >  1:2745873/1‑150 (MQ=255)
                                                         tCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTAt                                                                                         >  2:1609478/1‑150 (MQ=255)
                                                          cTTTTTGGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTAtt                                                                                        <  2:2785985/150‑1 (MQ=255)
                                                           ttttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTg                                                                                       >  1:814593/1‑150 (MQ=255)
                                                           ttttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTg                                                                                       >  1:2602829/1‑150 (MQ=255)
                                                           ttttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGGATGGGGGCGAAAAAGGTGATGGTCTTg                                                                                       >  2:3871992/1‑150 (MQ=255)
                                                            tttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGa                                                                                      >  1:167465/1‑150 (MQ=255)
                                                             ttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGAt                                                                                     >  1:1593847/1‑150 (MQ=255)
                                                              tttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATc                                                                                    >  2:1596868/1‑150 (MQ=255)
                                                               ttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGAGCGAAAAAGGTGATGGTATTGATcc                                                                                   >  1:1068032/1‑150 (MQ=255)
                                                                 ggTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAg                                                                                 >  2:798134/1‑150 (MQ=255)
                                                                  gTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGAGAAAAAGGTGATGGTATTGATCCAGc                                                                                <  2:2840539/150‑1 (MQ=255)
                                                                  gTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGc                                                                                >  2:1840563/1‑150 (MQ=255)
                                                                      tGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTcg                                                                            <  1:387181/150‑1 (MQ=255)
                                                                       gCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTcgc                                                                           >  1:4089821/1‑150 (MQ=255)
                                                                       gCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTcgc                                                                           >  1:738859/1‑150 (MQ=255)
                                                                        cGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTCTGGGTGCGAAAAAGGTGAGGGTATTGATCCAGCGTcgcg                                                                          >  2:1142230/1‑150 (MQ=255)
                                                                         gTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCgcgc                                                                         <  2:2781766/150‑1 (MQ=255)
                                                                            ggATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTa                                                                      <  1:446032/150‑1 (MQ=255)
                                                                             gATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGACAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAt                                                                     >  2:3410480/1‑150 (MQ=255)
                                                                              aTCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg                                                                    >  1:3140405/1‑150 (MQ=255)
                                                                                  aGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGa                                                                >  1:752004/1‑150 (MQ=255)
                                                                                   gAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGAt                                                               <  2:85486/150‑1 (MQ=255)
                                                                                    aaCTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATc                                                              >  2:1353820/1‑150 (MQ=255)
                                                                                           ggCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCa                                                       <  1:3257516/150‑1 (MQ=255)
                                                                                            gCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAg                                                      <  1:10203/150‑1 (MQ=255)
                                                                                             ccGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAgg                                                     >  2:3626791/1‑150 (MQ=255)
                                                                                             ccGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAgg                                                     <  2:205569/150‑1 (MQ=255)
                                                                                                aagaaCATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGa                                                  <  1:2619295/150‑1 (MQ=255)
                                                                                                aagaaCATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGa                                                  <  1:2345715/150‑1 (MQ=255)
                                                                                                aagaaCATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGa                                                  <  1:1295332/150‑1 (MQ=255)
                                                                                                 agaaCATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAg                                                 <  1:2371096/150‑1 (MQ=255)
                                                                                                      atatCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAATGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTa                                            >  2:1072875/1‑150 (MQ=255)
                                                                                                       tatCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTAt                                           >  2:1400457/1‑150 (MQ=255)
                                                                                                        atCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATc                                          >  2:1514083/1‑150 (MQ=255)
                                                                                                        atCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATc                                          <  1:1789667/150‑1 (MQ=255)
                                                                                                         tCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCg                                         <  2:2742511/150‑1 (MQ=255)
                                                                                                          cGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGa                                        <  1:4058967/150‑1 (MQ=255)
                                                                                                            aTCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGata                                      >  1:294896/1‑150 (MQ=255)
                                                                                                             tCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGatat                                     >  2:2042441/1‑150 (MQ=255)
                                                                                                               aaGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTg                                   <  2:2219088/150‑1 (MQ=255)
                                                                                                                  ttGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGa                                <  1:898546/150‑1 (MQ=255)
                                                                                                                   tGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGAt                               >  2:2294874/1‑150 (MQ=255)
                                                                                                                    gATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGAtt                              >  1:1840879/1‑150 (MQ=255)
                                                                                                                       cTGTTGATTGCCGTCACCTACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCGAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTca                           <  2:3662105/150‑1 (MQ=255)
                                                                                                                         gTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTcacc                         >  2:1027725/1‑150 (MQ=255)
                                                                                                                          tttATTGCCGTCACCCACGCTGACGAGGCCAATATCATGTCCGCCATGCTTGCCAATCGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATTTGGATCTGGTTCAGGGGAGCGTGATCGATATTGCGATTTcacca                        <  1:3755854/150‑1 (MQ=255)
                                                                                                                          tttATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTcacca                        >  2:2650744/1‑150 (MQ=255)
                                                                                                                          tttATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTcacca                        >  2:1341513/1‑150 (MQ=255)
                                                                                                                          tttATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACAGATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTcacca                        <  2:3628217/150‑1 (MQ=255)
                                                                                                                             aTTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCacaa                     >  1:1349045/1‑150 (MQ=255)
                                                                                                                              ttGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCacaac                    >  1:3428638/1‑150 (MQ=255)
                                                                                                                              ttGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTGATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCacaac                    >  1:2574044/1‑150 (MQ=255)
                                                                                                                               tGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTTTGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCTCCacaact                   >  2:2422699/1‑149 (MQ=255)
                                                                                                                               tGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCacaaca                   >  2:3368464/1‑150 (MQ=255)
                                                                                                                                 ccGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAg                 >  2:3028235/1‑150 (MQ=255)
                                                                                                                                 ccGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAg                 >  1:3502374/1‑150 (MQ=255)
                                                                                                                                  cGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGc                >  1:2252119/1‑150 (MQ=255)
                                                                                                                                      aCCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACt            >  1:758246/1‑150 (MQ=255)
                                                                                                                                      aCCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACt            >  1:2406365/1‑150 (MQ=255)
                                                                                                                                      aCCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACt            >  1:3977433/1‑150 (MQ=255)
                                                                                                                                        cAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACTAt          >  2:3885796/1‑150 (MQ=255)
                                                                                                                                        cAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACTAt          >  2:3403271/1‑150 (MQ=255)
                                                                                                                                         aaCGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCTGGGGAGCGTTATCGATATTGCGATTTCTCCACAACAAGCAACTAtt         >  2:3785849/1‑150 (MQ=255)
                                                                                                                                          aCGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACTAttt        >  1:2261470/1‑150 (MQ=255)
                                                                                                                                                aCGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACTATTTCTGCGt  <  2:434214/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TCGAACGTAATCAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACTATTTCTGCGT  >  NC_000913/3437296‑3437589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: