Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,078,297 Δ1 bp 7.4% intergenic (+190/+252) yeeW → / ← yoeD CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,078,2970C. 7.4% 216.8 / 14.9 81intergenic (+190/+252)yeeW/yoeDCP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (43/32);  new base . (4/2);  total (47/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

AGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGA  >  NC_000913/2078151‑2078444
                                                                                                                                                  |                                                                                                                                                    
aGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCt                                                                                                                                                    >  1:428283/1‑149 (MQ=255)
  ggTATAGTGCCATTAACCACAGAACCAGCACTGTTGTCCTGTATTAAGCAGGCAAGATAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACACACCAGGCTTCATTACTCCAGACGCCAGCCTGa                                                                                                                                                  >  1:285436/1‑149 (MQ=255)
    tataGTGCAATTAACCACAGAAACAGCACTGTTGTCCTGTATTAATCAGGCAAGAGAAGAAGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAAt                                                                                                                                                <  1:673134/149‑1 (MQ=255)
        gTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAg                                                                                                                                           <  1:299444/149‑1 (MQ=255)
         tGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCCCCAGAACCGGTGCATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGCATCCAgg                                                                                                                                          >  1:1118240/1‑148 (MQ=255)
                acccACTGAAACATCACTGTTGTGCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAAGCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCTGTATTCAGGCTGGCGtc                                                                                                                                   <  1:1030872/147‑2 (MQ=255)
                aaCCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCTGTATTCAGGCTGGCGtc                                                                                                                                   <  1:998121/149‑2 (MQ=255)
                     caGAAACAGCAATGTTGTCCTGTATTAATCAGGCAAGAGCAGATGTCCTTACCGTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCCATGCTCCTGACGCCACCCTGCTTACAGCTCGAGTTTTCAt                                                                                                                              >  1:649220/1‑149 (MQ=255)
                      aGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCAtt                                                                                                                             >  1:342585/1‑149 (MQ=255)
                         aaCAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACCCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTtat                                                                                                                          <  1:324961/149‑1 (MQ=255)
                          aCAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTtata                                                                                                                         >  2:449601/1‑149 (MQ=255)
                          aCAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTtata                                                                                                                         >  2:201137/1‑149 (MQ=255)
                              cACTGTTGTCCTGTATTAAGCAGGCAAGATAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACa                                                                                                                     <  1:660242/149‑1 (MQ=255)
                              cACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCTTTTCTCCTGACGCCAGCCTGAATACAGCTGTCGTTTTCATTTATtaaaa                                                                                                                     >  2:80456/1‑147 (MQ=255)
                                cTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGa                                                                                                                   <  2:192310/149‑1 (MQ=255)
                                cTGTAGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGa                                                                                                                   >  2:20774/1‑149 (MQ=255)
                                   ttgtCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAg                                                                                                                <  2:444484/149‑1 (MQ=255)
                                     gtCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTACTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGa                                                                                                              <  2:16235/149‑1 (MQ=255)
                                                aGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataat                                                                                                   >  2:57882/1‑149 (MQ=255)
                                                aGCAGGCAAGAGAAGATCTCGTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCTGTCAATCTCACTCAACAGGCTTCATTTCACCTGACGCCAGCCTTAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataat                                                                                                   <  1:808265/149‑1 (MQ=255)
                                                 gCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGATAACCGataata                                                                                                  >  2:573365/1‑149 (MQ=255)
                                                 gCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataata                                                                                                  >  2:909908/1‑149 (MQ=255)
                                                  cAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGAtaataa                                                                                                 >  1:1105594/1‑149 (MQ=255)
                                                    ggCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatg                                                                                               <  1:273432/149‑1 (MQ=255)
                                                     gCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatgg                                                                                              >  1:1103243/1‑149 (MQ=255)
                                                        agagTAGATGTCCTTTCCCTGCGCCATCTGCAGATTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatggaat                                                                                           <  1:118164/149‑1 (MQ=255)
                                                        agagAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatggaat                                                                                           <  2:342585/149‑1 (MQ=255)
                                                            aagaTGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGAc                                                                                       >  1:571722/1‑149 (MQ=255)
                                                                   ccTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATTAACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCgc                                                                                >  2:507469/1‑148 (MQ=255)
                                                                   ccTTACCCTGCGCAATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGa                                                                                <  2:369248/149‑1 (MQ=255)
                                                                    cTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCGGGCGTTTGCAGTTTTTAACAGAAAGGAAAACCGATAAGAATGGAATGGACGCGCCGAc                                                                               >  1:272114/1‑149 (MQ=255)
                                                                     ttACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAcc                                                                              <  2:1111893/149‑1 (MQ=255)
                                                                     cttaccTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATATTGGAATGGACGCCCCGAcc                                                                              >  2:111615/5‑149 (MQ=255)
                                                                        cccTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATg                                                                           <  2:1103243/149‑1 (MQ=255)
                                                                          cTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATTATAATGGAATGGACGCCCCGACCATGaa                                                                         >  1:225725/1‑149 (MQ=255)
                                                                              gcCATCTGCAGCTTCTGCACCAGAACCGGTGCATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAACACCGATAATAATGGAATGGCCGCCCCGCCCTTGAAGcca                                                                     >  2:794633/1‑147 (MQ=255)
                                                                              gcCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAATCAGAAAGGAAAACCGATAATAATGGAATGGACGCGCCGACCATGAAGCaa                                                                     >  2:962638/1‑149 (MQ=255)
                                                                                    tGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAg                                                               <  2:920735/149‑1 (MQ=255)
                                                                                      cAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATATACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCGACCATGACGCAACGGAAGGa                                                             >  2:787221/1‑149 (MQ=255)
                                                                                       aGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCGACCATGAAGCAACGGTAGGat                                                            >  1:148999/1‑149 (MQ=255)
                                                                                          ttCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATAtt                                                         >  1:441587/1‑149 (MQ=255)
                                                                                           tCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTa                                                        <  1:55050/149‑1 (MQ=255)
                                                                                             ttcaacAGAACCGGTTAATCTGACTCAACAGGCTTTATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACt                                                      <  1:902276/144‑1 (MQ=255)
                                                                                              gCACCATAACCGGTGAATCTCACTCAACAGGCTTAAAATCTCCTCACGCCAACCTCAAAACATCTGGCGTTTTCATTTATAAACAGAAAGCAAAACCGAAAATAATCGAATGTACTCCCCGACCATAAAGCGACGGTAGAATATTACtt                                                     >  2:209347/1‑148 (MQ=255)
                                                                                               cACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCAGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGa                                                    <  1:575464/149‑1 (MQ=255)
                                                                                                aCCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCAGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGaa                                                   >  1:323300/1‑148 (MQ=255)
                                                                                                 ccAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATa                                                  >  2:142517/1‑149 (MQ=255)
                                                                                                  cAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATaa                                                 >  1:969456/1‑149 (MQ=255)
                                                                                                  cAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACACCCCGACCATGCAGCAACGGTAGGATATTACTGATaa                                                 >  1:979054/1‑149 (MQ=255)
                                                                                                    gAACCGGTGAATCTCACTCAACAGGATTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAAc                                               <  2:746710/149‑1 (MQ=255)
                                                                                                       cccgTGCATCTCACACAACAGGCTTCATGTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTAATAAACACAAAGGAAAACCGATAATAAGGGAATGGACGCCCCGACCATCAACCAACGGTACGATATTACTGATACAccat                                            >  2:661740/4‑147 (MQ=255)
                                                                                                          gTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCCTTTTCATTTATAAACAGAAAGGAAAACCGATAAAAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGAAATTACTGATAAACCATCCa                                         >  1:90945/1‑149 (MQ=255)
                                                                                                               tctcACTCAACATGCTTCATTTCTCCTGACGCCAGCCTGAATCCAGCTGGCGTGTTCATTTATAAACAGAAATGAACACCGATAATAATGGAATGGACGCCCCGACCATGAGGCAACGGTAGGATATTACTGATAAACCTTACatttga                                    >  1:1185249/1‑144 (MQ=255)
                                                                                                                ctcactcaACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAg                                   >  2:604416/1‑149 (MQ=255)
                                                                                                                 acactcaACATGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAgg                                  <  1:242933/148‑1 (MQ=255)
                                                                                                                      ccacccgCTTCAATTAACCTCCCGCCAGCCAGCATCCCGCTGGCGTTTTCATTTATCAACCCAAACGAACCCCGAAAATAATGGCATGGACGCCCCGACCATGAAGCAACGGTAGGATATTCCTGATAAACCTTCCAGTGGAGGGGCGt                             <  2:533100/143‑1 (MQ=255)
                                                                                                                      caACAGTCTTCATTTCTCCTGACGACAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGATAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGt                             <  1:747231/149‑1 (MQ=255)
                                                                                                                       aaCAGGCTTCAATTCTCATGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGtt                            <  2:873023/149‑1 (MQ=255)
                                                                                                                        aCAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAACCAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAGCGGTAGGATATTACTGTTAAACCTTCCTGTGGAGGTGCGttt                           >  2:705750/1‑148 (MQ=255)
                                                                                                                         cAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACTCCCCGACCATGGAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTAt                          >  1:1017996/1‑149 (MQ=255)
                                                                                                                         cAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTAt                          <  1:142517/149‑1 (MQ=255)
                                                                                                                           ggCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATgg                        <  2:571722/149‑1 (MQ=255)
                                                                                                                           ggCTTCATTTCTCCTGACGCCAGCCTGAATACAGCGGGCGTGAGCATTTATAAACAGAAAGGAAAACCGAAAATAATGGAATGGACGCCCCGACCATGAACCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATgg                        <  1:798072/149‑1 (MQ=255)
                                                                                                                              ttCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACTGTAGTATAATACTGATAAACCTTCCACTGGAGGTGCGTTATGGCCg                     >  2:797269/1‑149 (MQ=255)
                                                                                                                                  tttCTCCTGACGCCAGCCTTAATACATCTGGAGTTTTCATTTATAAACAGAAAGGAAAACCTATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGtttt                 <  2:393674/149‑1 (MQ=255)
                                                                                                                                     atgaaaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAAc              >  2:1146621/7‑149 (MQ=255)
                                                                                                                                     atgaaaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATTTTACTGATAAACCTTCCAGGGGAGGTGCGTTATGGCCGTTTTAAc              >  2:190202/7‑149 (MQ=255)
                                                                                                                                      tcCTGACGCCAGCCTGAATACAGCTGCCGTTTTCATTTTTAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGGAACGGTAGGATATTACTGATAAACCGACCAGTGGACGTGAGTTATGGGCGTTTTTACg             >  1:484528/1‑149 (MQ=255)
                                                                                                                                         tGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGAtt          >  1:203839/1‑149 (MQ=255)
                                                                                                                                         tGACGCCAGCCTGAATACAGCTGGCGTTTTCATGTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTAAACGAtt          >  1:1168592/1‑149 (MQ=255)
                                                                                                                                         aaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTATCGAtt          >  1:154886/3‑149 (MQ=255)
                                                                                                                                         aaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGAtt          >  2:462953/3‑149 (MQ=255)
                                                                                                                                          gACGCCAGCCTGAATACTGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAACATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTa         <  2:285436/149‑1 (MQ=255)
                                                                                                                                           aCGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAg        >  1:285508/1‑149 (MQ=255)
                                                                                                                                            cGCCATCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAATACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAAGGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGc       <  1:20774/149‑1 (MQ=255)
                                                                                                                                             gCCAGCCTGAATACAGCTGTCGTTTTCATTTATAAACACAAAGGAAAACCGATAATAATGGAATTGACGCCCCGACCATGAAGCAACGGTATGATTTTACTGATAAACCTTCCAGTGGAGGTGCGTTATGTCCGTTTTAACGATTAGCa      <  2:620205/149‑1 (MQ=255)
                                                                                                                                               cAGCCTGAATACAGCTGGCGGTTTCATTTATAAACAGAAAGGACAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCCAGGGTAGGATTTGTCTGATAAACATTCCAGTGGAGGTGCGTTATGGCCGGTTTAACGATTAgaagt    >  2:24190/1‑145 (MQ=255)
                                                                                                                                                 gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGa  <  1:449601/149‑1 (MQ=255)
                                                                                                                                                 gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGa  >  2:922287/1‑149 (MQ=255)
                                                                                                                                                 gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGa  <  1:201137/149‑1 (MQ=255)
                                                                                                                                                 gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAAAGATTAGCATTGa  >  2:922285/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGA  >  NC_000913/2078151‑2078444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: