Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,541,626 Δ1 bp 10.3% coding (965/3741 nt) narZ ← nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,541,6260T.10.3% 95.9 / 7.7 39coding (965/3741 nt)narZnitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (16/19);  new base . (2/2);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-01

TTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCT  >  NC_000913/1541482‑1541769
                                                                                                                                                |                                                                                                                                               
ttgGTTTAGCCCAGTCCTTCCACCAGGTCAGATGCGCGGATCTTGCGACCGGGAACGTAGCTTCCATCGTCGGGTGGCTCCAGCATTACCAGCATCGGCATGACGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATc                                                                                                                                             <  1:142210/149‑1 (MQ=255)
ttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATc                                                                                                                                             >  1:292424/1‑149 (MQ=255)
ttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATc                                                                                                                                             >  1:372805/1‑149 (MQ=255)
ttgCTTTCGCCCAGTCCATCCACCAGGGCCGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCGCCAGCATTACCAGCATCGGCATCTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATc                                                                                                                                             <  2:698423/149‑1 (MQ=255)
 tgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCAGGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCg                                                                                                                                            <  2:839392/149‑1 (MQ=255)
  gCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCCGCATGTAGCTGTAGCGGCGGCAGTAGTTGATAATGTAGTCGCTGGGATTATCga                                                                                                                                           >  1:544253/1‑149 (MQ=255)
   cTTTCGCCCAGTCCATCCCCCAGGTCAGATCCGCGGAGCATGCCCCCGGGAACGTAGCAACCATCGTCGCGAGGCTCCAGCCTTACCAGCAGCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCgag                                                                                                                                          <  2:783109/149‑1 (MQ=255)
        gCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGA‑TATCGAGATGaaa                                                                                                                                    >  2:730446/1‑149 (MQ=255)
        gCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGA‑TATCGAGAGGaaa                                                                                                                                    >  2:726138/1‑149 (MQ=255)
                                  cgcgGATCATGCGCCCGGGTACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCTGCAGTAGTTGATAAAGTAGTCGCGGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGTCCAtt                                                                                                           <  1:398812/149‑1 (MQ=255)
                                        tCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATc                                                                                                     <  1:453927/149‑1 (MQ=255)
                                         cATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGA‑TATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGc                                                                                                   <  1:726138/149‑1 (MQ=255)
                                         cATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGA‑TATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGc                                                                                                   <  1:730446/149‑1 (MQ=255)
                                             cgcCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCac                                                                                                >  1:706549/1‑148 (MQ=255)
                                               cccGGGAACGTAGCTACCATCGTCGCGAGGCGCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAggg                                                                                              <  2:341812/149‑1 (MQ=255)
                                                ccGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGgc                                                                                             <  2:467441/149‑1 (MQ=255)
                                                                 aTCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGtt                                                                            >  1:571855/1‑149 (MQ=255)
                                                                  tcgtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGttt                                                                           >  2:973515/1‑149 (MQ=255)
                                                                     tcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCTGTAGTTGATAAAGTAGTCGGTGGGATTATCGAGATGAAACTCTTTTATAATCACATGGCCCATTGCCATCGCCAGGGCGGTATCAGTGCCTTGGTTCgg                                                                        >  2:332960/1‑149 (MQ=255)
                                                                      cgcgAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGt                                                                       <  1:233976/149‑1 (MQ=255)
                                                                      cgcgAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGt                                                                       <  2:830935/149‑1 (MQ=255)
                                                                       gcgAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAGCTCTTGTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTg                                                                      >  1:851626/1‑149 (MQ=255)
                                                                        cgAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGATACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgc                                                                     <  2:965701/149‑1 (MQ=255)
                                                                               ccAGCATTACCAGCATCGGCATGTCGGTGTAGCGGCGGCAGTAGTTGATAAAGTCGTCGCTGGGATTATCGAGATGAAACTCTTTTTAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCAc                                                              <  1:800901/149‑1 (MQ=255)
                                                                                cAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGAGAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACt                                                             >  2:1134348/1‑149 (MQ=255)
                                                                                        cccgCATCGGCATGTCGCTGTAGCGGCGGCAGGTGTTGATAACGTACGCGCTGGGATTATCGAGATGAAACTCTATTAAAATCACATGCCCCATTGCCATCCCCAGGGCGCTATCAGAGCCTTGTTCCGGTGCCAGCCACTGGTCGcac                                                     <  2:82932/146‑1 (MQ=255)
                                                                                         cAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGcaca                                                    >  1:1114038/1‑149 (MQ=255)
                                                                                            cATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAAtt                                                 <  2:559118/149‑1 (MQ=255)
                                                                                                   aTGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCAc                                          <  2:372805/149‑1 (MQ=255)
                                                                                                   aTGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCAc                                          <  2:292424/149‑1 (MQ=255)
                                                                                                   aTGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGACAGCCACTGGTCGCCCAAGTTGGCCAc                                          >  2:919907/1‑149 (MQ=255)
                                                                                                     gTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACtt                                        >  1:827881/1‑149 (MQ=255)
                                                                                                       cGCTGTAGCGGCGGCAGTAGTTGATTAAGTAGTCGCTGGGATTATCGATATGTAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCa                                      <  2:656208/149‑1 (MQ=255)
                                                                                                         cTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCaga                                    <  1:548683/149‑1 (MQ=255)
                                                                                                            tAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCTGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGagta                                 <  1:130304/149‑1 (MQ=255)
                                                                                                            tAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGagta                                 >  1:922194/1‑149 (MQ=255)
                                                                                                                          gtTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATgg                   >  1:220303/1‑149 (MQ=255)
                                                                                                                             gATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCgt                >  1:395767/1‑148 (MQ=255)
                                                                                                                                           tGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCCCAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCt  >  2:1170452/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCT  >  NC_000913/1541482‑1541769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑