Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,078,306:1 +G 7.8% intergenic (+199/+243) yeeW → / ← yoeD CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,078,3061.G 7.8% 303.7 / 23.3 77intergenic (+199/+243)yeeW/yoeDCP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base . (40/31);  new base G (4/2);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

GTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTG  >  NC_000913/2078159‑2078448
                                                                                                                                                    |                                                                                                                                              
gTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAg                                                                                                                                               <  1:299444/149‑1 (MQ=255)
 tGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCCCCAGAACCGGTGCATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGCATCCAgg                                                                                                                                              >  1:1118240/1‑148 (MQ=255)
        acccACTGAAACATCACTGTTGTGCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAAGCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCTGTATTCAGGCTGGCGtc                                                                                                                                       <  1:1030872/147‑2 (MQ=255)
        aaCCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCTGTATTCAGGCTGGCGtc                                                                                                                                       <  1:998121/149‑2 (MQ=255)
             caGAAACAGCAATGTTGTCCTGTATTAATCAGGCAAGAGCAGATGTCCTTACCGTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCCATGCTCCTGACGCCACCCTGCTTACAGCTCGAGTTTTCAt                                                                                                                                  >  1:649220/1‑149 (MQ=255)
              aGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCAtt                                                                                                                                 >  1:342585/1‑149 (MQ=255)
                 aaCAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACCCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTtat                                                                                                                              <  1:324961/149‑1 (MQ=255)
                  aCAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTtata                                                                                                                             >  2:449601/1‑149 (MQ=255)
                  aCAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTtata                                                                                                                             >  2:201137/1‑149 (MQ=255)
                      cACTGTTGTCCTGTATTAAGCAGGCAAGATAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACa                                                                                                                         <  1:660242/149‑1 (MQ=255)
                      cACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCTTTTCTCCTGACGCCAGCCTGAATACAGCTGTCGTTTTCATTTATtaaaa                                                                                                                         >  2:80456/1‑147 (MQ=255)
                        cTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGa                                                                                                                       <  2:192310/149‑1 (MQ=255)
                        cTGTAGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGa                                                                                                                       >  2:20774/1‑149 (MQ=255)
                           ttgtCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAg                                                                                                                    <  2:444484/149‑1 (MQ=255)
                             gtCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTACTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGa                                                                                                                  <  2:16235/149‑1 (MQ=255)
                                        aGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataat                                                                                                       >  2:57882/1‑149 (MQ=255)
                                        aGCAGGCAAGAGAAGATCTCGTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCTGTCAATCTCACTCAACAGGCTTCATTTCACCTGACGCCAGCCTTAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataat                                                                                                       <  1:808265/149‑1 (MQ=255)
                                         gCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGATAACCGataata                                                                                                      >  2:573365/1‑149 (MQ=255)
                                         gCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataata                                                                                                      >  2:909908/1‑149 (MQ=255)
                                          cAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGAtaataa                                                                                                     >  1:1105594/1‑149 (MQ=255)
                                            ggCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatg                                                                                                   <  1:273432/149‑1 (MQ=255)
                                             gCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatgg                                                                                                  >  1:1103243/1‑149 (MQ=255)
                                                agagTAGATGTCCTTTCCCTGCGCCATCTGCAGATTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatggaat                                                                                               <  1:118164/149‑1 (MQ=255)
                                                agagAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatggaat                                                                                               <  2:342585/149‑1 (MQ=255)
                                                    aagaTGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGAc                                                                                           >  1:571722/1‑149 (MQ=255)
                                                           ccTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATTAACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCgc                                                                                    >  2:507469/1‑148 (MQ=255)
                                                           ccTTACCCTGCGCAATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGa                                                                                    <  2:369248/149‑1 (MQ=255)
                                                            cTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCGGGCGTTTGCAGTTTTTAACAGAAAGGAAAACCGATAAGAATGGAATGGACGCGCCGAc                                                                                   >  1:272114/1‑149 (MQ=255)
                                                             ttACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAcc                                                                                  <  2:1111893/149‑1 (MQ=255)
                                                             cttaccTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATATTGGAATGGACGCCCCGAcc                                                                                  >  2:111615/5‑149 (MQ=255)
                                                                cccTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATg                                                                               <  2:1103243/149‑1 (MQ=255)
                                                                  cTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATTATAATGGAATGGACGCCCCGACCATGaa                                                                             >  1:225725/1‑149 (MQ=255)
                                                                      gcCATCTGCAGCTTCTGCACCAGAACCGGTGCATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAACACCGATAATAATGGAATGGCCGCCCCGCCCTTGAAGcca                                                                         >  2:794633/1‑147 (MQ=255)
                                                                      gcCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAATCAGAAAGGAAAACCGATAATAATGGAATGGACGCGCCGACCATGAAGCaa                                                                         >  2:962638/1‑149 (MQ=255)
                                                                            tGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAg                                                                   <  2:920735/149‑1 (MQ=255)
                                                                              cAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATATACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCGACCATGACGCAACGGAAGGa                                                                 >  2:787221/1‑149 (MQ=255)
                                                                               aGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCGACCATGAAGCAACGGTAGGat                                                                >  1:148999/1‑149 (MQ=255)
                                                                                  ttCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATAtt                                                             >  1:441587/1‑149 (MQ=255)
                                                                                   tCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTa                                                            <  1:55050/149‑1 (MQ=255)
                                                                                     ttcaacAGAACCGGTTAATCTGACTCAACAGGCTTTATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACt                                                          <  1:902276/144‑1 (MQ=255)
                                                                                      gCACCATAACCGGTGAATCTCACTCAACAGGCTTAAAATCTCCTCACGCCAACCTCAAAACATCTGGCGTTTTCATTTATAAACAGAAAGCAAAACCGAAAATAATCGAATGTACTCCCCGACCATAAAGCGACGGTAGAATATTACtt                                                         >  2:209347/1‑148 (MQ=255)
                                                                                       cACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCAGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGa                                                        <  1:575464/149‑1 (MQ=255)
                                                                                        aCCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCAGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGaa                                                       >  1:323300/1‑148 (MQ=255)
                                                                                         ccAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATa                                                      >  2:142517/1‑149 (MQ=255)
                                                                                          cAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATaa                                                     >  1:969456/1‑149 (MQ=255)
                                                                                          cAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACACCCCGACCATGCAGCAACGGTAGGATATTACTGATaa                                                     >  1:979054/1‑149 (MQ=255)
                                                                                            gAACCGGTGAATCTCACTCAACAGGATTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAAc                                                   <  2:746710/149‑1 (MQ=255)
                                                                                               cccgTGCATCTCACACAACAGGCTTCATGTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTAATAAACACAAAGGAAAACCGATAATAAGGGAATGGACGCCCCGACCATCAACCAACGGTACGATATTACTGATACAccat                                                >  2:661740/4‑147 (MQ=255)
                                                                                                  gTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCCTTTTCATTTATAAACAGAAAGGAAAACCGATAAAAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGAAATTACTGATAAACCATCCa                                             >  1:90945/1‑149 (MQ=255)
                                                                                                       tctcACTCAACATGCTTCATTTCTCCTGACGCCAGCCTGAATCCAGCTGGCGTGTTCATTTATAAACAGAAATGAACACCGATAATAATGGAATGGACGCCCCGACCATGAGGCAACGGTAGGATATTACTGATAAACCTTACatttga                                        >  1:1185249/1‑144 (MQ=255)
                                                                                                        ctcactcaACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAg                                       >  2:604416/1‑149 (MQ=255)
                                                                                                         acactcaACATGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAgg                                      <  1:242933/148‑1 (MQ=255)
                                                                                                              ccacccgCTTCAATTAACCTCCCGCCAGCCAGCATCCCGCTGGCGTTTTCATTTATCAACCCAAACGAACCCCGAAAATAATGGCATGGACGCCCCGACCATGAAGCAACGGTAGGATATTCCTGATAAACCTTCCAGTGGAGGGGCGt                                 <  2:533100/143‑1 (MQ=255)
                                                                                                              caACAGTCTTCATTTCTCCTGACGACAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGATAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGt                                 <  1:747231/149‑1 (MQ=255)
                                                                                                               aaCAGGCTTCAATTCTCATGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGtt                                <  2:873023/149‑1 (MQ=255)
                                                                                                                aCAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAACCAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAGCGGTAGGATATTACTGTTAAACCTTCCTGTGGAGGTGCGttt                               >  2:705750/1‑148 (MQ=255)
                                                                                                                 cAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACTCCCCGACCATGGAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTAt                              >  1:1017996/1‑149 (MQ=255)
                                                                                                                 cAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTAt                              <  1:142517/149‑1 (MQ=255)
                                                                                                                   ggCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATgg                            <  2:571722/149‑1 (MQ=255)
                                                                                                                   ggCTTCATTTCTCCTGACGCCAGCCTGAATACAGCGGGCGTGAGCATTTATAAACAGAAAGGAAAACCGAAAATAATGGAATGGACGCCCCGACCATGAACCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATgg                            <  1:798072/149‑1 (MQ=255)
                                                                                                                      ttCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACTGTAGTATAATACTGATAAACCTTCCACTGGAGGTGCGTTATGGCCg                         >  2:797269/1‑149 (MQ=255)
                                                                                                                          tttCTCCTGACGCCAGCCTTAATACATCTGGAGTTTTCATTTATAAACAGAAAGGAAAACCTATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGtttt                     <  2:393674/149‑1 (MQ=255)
                                                                                                                             atgaaaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAAc                  >  2:1146621/7‑149 (MQ=255)
                                                                                                                             atgaaaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATTTTACTGATAAACCTTCCAGGGGAGGTGCGTTATGGCCGTTTTAAc                  >  2:190202/7‑149 (MQ=255)
                                                                                                                              tcCTGACGCCAGCCTGAATACAGCTGCCGTTTTCATTTTTAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGGAACGGTAGGATATTACTGATAAACCGACCAGTGGACGTGAGTTATGGGCGTTTTTACg                 >  1:484528/1‑149 (MQ=255)
                                                                                                                                 tGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGAtt              >  1:203839/1‑149 (MQ=255)
                                                                                                                                 tGACGCCAGCCTGAATACAGCTGGCGTTTTCATGTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTAAACGAtt              >  1:1168592/1‑149 (MQ=255)
                                                                                                                                 aaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTATCGAtt              >  1:154886/3‑149 (MQ=255)
                                                                                                                                 aaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGAtt              >  2:462953/3‑149 (MQ=255)
                                                                                                                                  gACGCCAGCCTGAATACTGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAACATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTa             <  2:285436/149‑1 (MQ=255)
                                                                                                                                   aCGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAg            >  1:285508/1‑149 (MQ=255)
                                                                                                                                    cGCCATCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAATACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAAGGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGc           <  1:20774/149‑1 (MQ=255)
                                                                                                                                     gCCAGCCTGAATACAGCTGTCGTTTTCATTTATAAACACAAAGGAAAACCGATAATAATGGAATTGACGCCCCGACCATGAAGCAACGGTATGATTTTACTGATAAACCTTCCAGTGGAGGTGCGTTATGTCCGTTTTAACGATTAGCa          <  2:620205/149‑1 (MQ=255)
                                                                                                                                       cAGCCTGAATACAGCTGGCGGTTTCATTTATAAACAGAAAGGACAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCCAGGGTAGGATTTGTCTGATAAACATTCCAGTGGAGGTGCGTTATGGCCGGTTTAACGATTAgaagt        >  2:24190/1‑145 (MQ=255)
                                                                                                                                         gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGa      <  1:449601/149‑1 (MQ=255)
                                                                                                                                         gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGa      <  1:201137/149‑1 (MQ=255)
                                                                                                                                         gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGa      >  2:922287/1‑149 (MQ=255)
                                                                                                                                         gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAAAGATTAGCATTGa      >  2:922285/1‑149 (MQ=255)
                                                                                                                                             taaTACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGCTAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTg  <  1:854096/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTG  >  NC_000913/2078159‑2078448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: