Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 14,116 A→G 6.3% intergenic (+37/‑52) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091314,1160AG 6.3% 281.9 / 6.1 97intergenic (+37/‑52)dnaK/dnaJchaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base A (45/44);  new base G (3/3);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATAC  >  NC_000913/13970‑14263
                                                                                                                                                  |                                                                                                                                                   
agcagcAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAg                                                                                                                                                   >  2:900965/1‑149 (MQ=255)
agcagcAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAg                                                                                                                                                   >  1:196856/1‑149 (MQ=255)
 gcagcaACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATACTCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAgg                                                                                                                                                  <  2:759005/149‑1 (MQ=255)
 gcagcaACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAgg                                                                                                                                                  <  1:144740/149‑1 (MQ=255)
      aaCATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAt                                                                                                                                             <  1:900213/149‑1 (MQ=255)
      aaCATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAt                                                                                                                                             <  1:284722/149‑1 (MQ=255)
       ccATGCCCAGCAGCAGACTGCCGGTGCTGCTGCTTCTGCAAACAACGCGAAAGATGACGATGTTGGCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATTAACGGGTAATTATACTGACACGGGCGAAGGGGAAtt                                                                                                                                            >  2:1041722/2‑149 (MQ=255)
       aCATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAtt                                                                                                                                            >  2:127057/1‑149 (MQ=255)
        cATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAAttt                                                                                                                                           >  2:363653/1‑149 (MQ=255)
         aTGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTc                                                                                                                                          >  2:611222/1‑149 (MQ=255)
         aTGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGGCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTc                                                                                                                                          <  1:35386/149‑1 (MQ=255)
           gCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCct                                                                                                                                        >  2:419303/1‑149 (MQ=255)
                 cagctgACTGCCTGTGCTGATGCTTCTGCAAACAACGCGATAGATGACGATGTTGGCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGc                                                                                                                                  <  1:548992/149‑1 (MQ=255)
                  agcagACTGCCGGTGCTTATGCTTCAGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAAAAATCGCCCTATAAACGGGTAAATATACTGACACGGGCGGAGAGGAAATTCCCCTTCGcc                                                                                                                                 <  2:1066817/149‑1 (MQ=255)
                   ccagACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGAGGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGccc                                                                                                                                <  1:1088900/148‑1 (MQ=255)
                    cagACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGCCGAAGGGGAATTTCCTCTCCGCCCg                                                                                                                               >  2:120117/1‑149 (MQ=255)
                     agACTGCCGTTGCCGATGCTTCTGCAAACAACGCGAAAGATGACGATGGCGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTCATTATCCTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGt                                                                                                                              <  1:1177769/149‑1 (MQ=255)
                       aCTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTATTTATACTGACACGGGCGAAGGGGAATTTCATCTCCTCCCGtcc                                                                                                                            >  1:791268/1‑147 (MQ=255)
                         tGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAt                                                                                                                          >  1:755644/1‑149 (MQ=255)
                                 cTGATGCTTCTGCAAACAACGCTAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATATCCTCTCCGCCCGTGCATTCATCTAg                                                                                                                  <  1:910689/149‑1 (MQ=255)
                                    aTGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAgggg                                                                                                               >  2:1001044/1‑149 (MQ=255)
                                        ttCTGCAAACAACGCGAAAGCTGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAAt                                                                                                           >  1:176426/1‑149 (MQ=255)
                                        ttCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAAt                                                                                                           >  1:1117416/1‑149 (MQ=255)
                                            gCAAACAACGCGAATGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAACGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGCGAATTTCCTCTCCGCCCTTGCATTCATCTAGGGGCTATTTaa                                                                                                       >  1:781892/1‑149 (MQ=255)
                                              aaaCAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaa                                                                                                     <  1:609637/149‑1 (MQ=255)
                                              aaaCAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaa                                                                                                     <  1:1058821/149‑1 (MQ=255)
                                                acaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaaaa                                                                                                   >  1:303851/1‑149 (MQ=255)
                                                acaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGCCGAAGGGGAATTTCGTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaaaa                                                                                                   >  2:363032/1‑149 (MQ=255)
                                                  aacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGa                                                                                                 <  2:723094/149‑1 (MQ=255)
                                                  aacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGa                                                                                                 >  1:873460/1‑149 (MQ=255)
                                                   acGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAt                                                                                                <  1:362852/149‑1 (MQ=255)
                                                       gAAATAAGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGCCAATAAATATTGGCCCTATAAACGGTTTATTATACTGACAAGGGCGAAGGGGAATTTCCTGTCCGCCCGTGCATTCATCTAGGGGCAATTTATAAATGATGGCt                                                                                            <  1:240475/149‑1 (MQ=255)
                                                       gAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCt                                                                                            >  1:942262/1‑149 (MQ=255)
                                                       gAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCATCTCCGCCCGTGCATTCATCTAGGGGCAATATAAAAAAGATGGCt                                                                                            >  1:307302/1‑149 (MQ=255)
                                                         aaGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTGTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTaa                                                                                          <  1:788177/149‑1 (MQ=255)
                                                          aGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAg                                                                                         >  2:778669/1‑149 (MQ=255)
                                                           gATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTGTAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGc                                                                                        <  1:372139/149‑1 (MQ=255)
                                                           gATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGc                                                                                        <  1:127057/149‑1 (MQ=255)
                                                              gACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAg                                                                                     >  2:204730/1‑149 (MQ=255)
                                                                cGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCTTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGat                                                                                   >  2:603976/1‑149 (MQ=255)
                                                                 gATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCAGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGatt                                                                                  >  2:1065607/1‑149 (MQ=255)
                                                                    gttgtCGACGCTGTATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAAGGGGTAATTATAGTGACTCGGGAGTAGGTGAATTTCCTCTCCGCCCGTGCATTCATCTATTGGCAATTTAAAAAAGATGGCTAAGCAAGattatt                                                                               <  2:526371/149‑1 (MQ=255)
                                                                    gttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGattatt                                                                               <  1:1127813/149‑1 (MQ=255)
                                                                     ttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACTGGTAATTATACTGACCCGGGCGAAGGGGAATTTCCTCTCCGCCGGTGCATTCTTCTAGGGGCAATTTAAAAAAGATGGGTAAGCAAGAttatta                                                                              <  2:995438/149‑1 (MQ=255)
                                                                      tgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTAc                                                                             <  1:224451/149‑1 (MQ=255)
                                                                       gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTGTCCGCCCGTGCATTAATCTAGGGCAAATTTAAAAAAGATGGCTAAGCATGTTTATTAcc                                                                            >  2:660934/1‑148 (MQ=255)
                                                                       gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACg                                                                            >  1:21593/1‑149 (MQ=255)
                                                                          gACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgaga                                                                         >  1:1177044/1‑149 (MQ=255)
                                                                           aCGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAt                                                                        >  1:261301/1‑149 (MQ=255)
                                                                              atcaaTTTCCAGAAGTCAATGAAAAAAAATAATCGCCCTAAAAACGGCTAATTATACTTACAAGTGCGAAGGGGAATTTCCTCTACGCCCGTGCATTCATCTAGTTGAAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAtttt                                                                     <  2:768308/146‑1 (MQ=255)
                                                                               tGAATTTGAAGAAGTCAAAGCCAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAGTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa                                                                    >  2:526896/1‑149 (MQ=255)
                                                                                 aaTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAgg                                                                  <  2:596181/149‑1 (MQ=255)
                                                                                 aaTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAgg                                                                  <  1:452322/149‑1 (MQ=255)
                                                                                   tttGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTgtca                                                                                                                          <  1:1132508/91‑4 (MQ=25)
                                                                                   tttGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTgtca                                                                                                                          >  2:1132508/1‑88 (MQ=25)
                                                                                     tGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGtt                                                              >  2:350661/1‑149 (MQ=255)
                                                                                      gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGttt                                                             >  1:494028/1‑149 (MQ=255)
                                                                                      gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGttt                                                             <  1:947493/149‑1 (MQ=255)
                                                                                      gaagaaGTCAAAGACAAAAAATAATCGCACTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCtttt                                                             >  2:302551/1‑149 (MQ=255)
                                                                                       aagaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTc                                                            <  1:172104/149‑1 (MQ=255)
                                                                                          aagTCAAACACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa                                                         <  2:568609/149‑1 (MQ=255)
                                                                                            gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa                                                       >  1:75820/1‑149 (MQ=255)
                                                                                            gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa                                                       <  1:290868/149‑1 (MQ=255)
                                                                                              cAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACa                                                     >  1:389424/1‑149 (MQ=255)
                                                                                              cAAAGACAAAAAATAAGCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAGGGCTACGCCAGATTATTACGAGATTTTAGGCGTTTCCAAAACa                                                     <  1:206645/149‑1 (MQ=255)
                                                                                               aaaGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAg                                                    <  2:656595/149‑1 (MQ=255)
                                                                                               aaaGACAAAAAATAATCGCCCTATAAACGGGTAATTATACGGACACCGGCGAAGGGGAATTTCCTCTCCGCCAGTGCATACTTCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACTAGAGTTTAGGATTTTCCAAAACat                                                    >  1:683795/1‑148 (MQ=255)
                                                                                                 aGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCg                                                  <  1:1109858/149‑1 (MQ=255)
                                                                                                  gACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCgg                                                 <  1:1001044/149‑1 (MQ=255)
                                                                                                   aCAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGTCGTTTCCACAACAGCGGa                                                >  1:178601/1‑149 (MQ=255)
                                                                                                    cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAGTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTATCCAAAACAGCGGaa                                               >  1:839428/1‑149 (MQ=255)
                                                                                                      aaaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAaga                                             <  2:923239/149‑1 (MQ=255)
                                                                                                       aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagac                                            >  2:418532/1‑148 (MQ=255)
                                                                                                       aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAGTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagag                                            >  2:889523/1‑149 (MQ=255)
                                                                                                       aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAATATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagac                                            >  1:1172851/1‑148 (MQ=255)
                                                                                                          ataatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGt                                         <  1:500939/149‑1 (MQ=255)
                                                                                                             atCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGaa                                      <  1:204730/149‑1 (MQ=255)
                                                                                                               cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt                                    <  1:363653/149‑1 (MQ=255)
                                                                                                                tcccTATAAACGGGTAATTATATTGACACGGGCGAAGGTGAATTTCCTCTCAGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATc                                   <  2:247476/148‑1 (MQ=255)
                                                                                                                    tataAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTACTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCTAGATTATTACTAGATTTTAGGAGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGaa                               >  1:59239/1‑149 (MQ=255)
                                                                                                                     ataAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGaaa                              >  1:1177249/1‑149 (MQ=255)
                                                                                                                         aCGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTTTTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGTcc                          >  1:1117218/1‑149 (MQ=255)
                                                                                                                          cGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCt                         <  1:724384/149‑1 (MQ=255)
                                                                                                                           gggTAATTATACTGACACGGGCGAAGGGGAATTTCGTCTCCGCCCGTGCATTCATCTAGGGGCAATATAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                        <  2:1163392/149‑1 (MQ=255)
                                                                                                                           gggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                        >  1:961842/1‑149 (MQ=255)
                                                                                                                           gggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                        <  1:937262/149‑1 (MQ=255)
                                                                                                                            ggTAACTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTAc                       <  1:550521/149‑1 (MQ=255)
                                                                                                                             gTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACa                      >  1:38847/1‑149 (MQ=255)
                                                                                                                                  tataCTGACACTGGCCTAGGGGAATTTCCTCTCCGCGCGTGCATTCATCTAGGCGCACTTTAAAAAAGCTGGCTTAGCAAGATTTTTACGAGATTTTCGGCGTTTCCAACACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGc                 <  2:898136/149‑1 (MQ=255)
                                                                                                                                  tataCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGc                 <  1:412355/149‑1 (MQ=255)
                                                                                                                                           acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATg        >  2:507573/1‑149 (MQ=255)
                                                                                                                                           acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATg        >  2:503152/1‑149 (MQ=255)
                                                                                                                                            cGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGa       <  2:746640/149‑1 (MQ=255)
                                                                                                                                             gggCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGaa      <  2:791268/149‑1 (MQ=255)
                                                                                                                                               gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAAt    >  2:447900/1‑149 (MQ=255)
                                                                                                                                                cGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATa   <  1:611222/149‑1 (MQ=255)
                                                                                                                                                 gAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATac  <  1:350661/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATAC  >  NC_000913/13970‑14263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: