Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,078,301 A→T 7.6% intergenic (+194/+248) yeeW → / ← yoeD CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,078,3010AT 7.6% 207.4 / 7.0 79intergenic (+194/+248)yeeW/yoeDCP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base A (39/31);  new base T (4/2);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

GGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTG  >  NC_000913/2078153‑2078448
                                                                                                                                                    |                                                                                                                                                    
ggTATAGTGCCATTAACCACAGAACCAGCACTGTTGTCCTGTATTAAGCAGGCAAGATAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACACACCAGGCTTCATTACTCCAGACGCCAGCCTGa                                                                                                                                                      >  1:285436/1‑149 (MQ=255)
  tataGTGCAATTAACCACAGAAACAGCACTGTTGTCCTGTATTAATCAGGCAAGAGAAGAAGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAAt                                                                                                                                                    <  1:673134/149‑1 (MQ=255)
      gTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAg                                                                                                                                               <  1:299444/149‑1 (MQ=255)
       tGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCCCCAGAACCGGTGCATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGCATCCAgg                                                                                                                                              >  1:1118240/1‑148 (MQ=255)
              acccACTGAAACATCACTGTTGTGCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAAGCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCTGTATTCAGGCTGGCGtc                                                                                                                                       <  1:1030872/147‑2 (MQ=255)
              aaCCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCTGTATTCAGGCTGGCGtc                                                                                                                                       <  1:998121/149‑2 (MQ=255)
                   caGAAACAGCAATGTTGTCCTGTATTAATCAGGCAAGAGCAGATGTCCTTACCGTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCCATGCTCCTGACGCCACCCTGCTTACAGCTCGAGTTTTCAt                                                                                                                                  >  1:649220/1‑149 (MQ=255)
                    aGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCAtt                                                                                                                                 >  1:342585/1‑149 (MQ=255)
                       aaCAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACCCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTtat                                                                                                                              <  1:324961/149‑1 (MQ=255)
                        aCAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTtata                                                                                                                             >  2:449601/1‑149 (MQ=255)
                        aCAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTtata                                                                                                                             >  2:201137/1‑149 (MQ=255)
                            cACTGTTGTCCTGTATTAAGCAGGCAAGATAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACa                                                                                                                         <  1:660242/149‑1 (MQ=255)
                            cACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCTTTTCTCCTGACGCCAGCCTGAATACAGCTGTCGTTTTCATTTATtaaaa                                                                                                                         >  2:80456/1‑147 (MQ=255)
                              cTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGa                                                                                                                       <  2:192310/149‑1 (MQ=255)
                              cTGTAGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGa                                                                                                                       >  2:20774/1‑149 (MQ=255)
                                 ttgtCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAg                                                                                                                    <  2:444484/149‑1 (MQ=255)
                                   gtCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTACTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGa                                                                                                                  <  2:16235/149‑1 (MQ=255)
                                              aGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataat                                                                                                       >  2:57882/1‑149 (MQ=255)
                                              aGCAGGCAAGAGAAGATCTCGTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCTGTCAATCTCACTCAACAGGCTTCATTTCACCTGACGCCAGCCTTAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataat                                                                                                       <  1:808265/149‑1 (MQ=255)
                                               gCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGATAACCGataata                                                                                                      >  2:573365/1‑149 (MQ=255)
                                               gCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataata                                                                                                      >  2:909908/1‑149 (MQ=255)
                                                cAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGAtaataa                                                                                                     >  1:1105594/1‑149 (MQ=255)
                                                  ggCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatg                                                                                                   <  1:273432/149‑1 (MQ=255)
                                                   gCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatgg                                                                                                  >  1:1103243/1‑149 (MQ=255)
                                                      agagTAGATGTCCTTTCCCTGCGCCATCTGCAGATTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatggaat                                                                                               <  1:118164/149‑1 (MQ=255)
                                                      agagAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatggaat                                                                                               <  2:342585/149‑1 (MQ=255)
                                                          aagaTGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGAc                                                                                           >  1:571722/1‑149 (MQ=255)
                                                                 ccTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATTAACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCgc                                                                                    >  2:507469/1‑148 (MQ=255)
                                                                 ccTTACCCTGCGCAATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGa                                                                                    <  2:369248/149‑1 (MQ=255)
                                                                  cTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCGGGCGTTTGCAGTTTTTAACAGAAAGGAAAACCGATAAGAATGGAATGGACGCGCCGAc                                                                                   >  1:272114/1‑149 (MQ=255)
                                                                   ttACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAcc                                                                                  <  2:1111893/149‑1 (MQ=255)
                                                                   cttaccTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATATTGGAATGGACGCCCCGAcc                                                                                  >  2:111615/5‑149 (MQ=255)
                                                                      cccTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATg                                                                               <  2:1103243/149‑1 (MQ=255)
                                                                        cTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATTATAATGGAATGGACGCCCCGACCATGaa                                                                             >  1:225725/1‑149 (MQ=255)
                                                                            gcCATCTGCAGCTTCTGCACCAGAACCGGTGCATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAACACCGATAATAATGGAATGGCCGCCCCGCCCTTGAAGcca                                                                         >  2:794633/1‑147 (MQ=255)
                                                                            gcCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAATCAGAAAGGAAAACCGATAATAATGGAATGGACGCGCCGACCATGAAGCaa                                                                         >  2:962638/1‑149 (MQ=255)
                                                                                  tGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAg                                                                   <  2:920735/149‑1 (MQ=255)
                                                                                    cAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATATACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCGACCATGACGCAACGGAAGGa                                                                 >  2:787221/1‑149 (MQ=255)
                                                                                     aGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCGACCATGAAGCAACGGTAGGat                                                                >  1:148999/1‑149 (MQ=255)
                                                                                        ttCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATAtt                                                             >  1:441587/1‑149 (MQ=255)
                                                                                         tCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTa                                                            <  1:55050/149‑1 (MQ=255)
                                                                                           ttcaacAGAACCGGTTAATCTGACTCAACAGGCTTTATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACt                                                          <  1:902276/144‑1 (MQ=255)
                                                                                            gCACCATAACCGGTGAATCTCACTCAACAGGCTTAAAATCTCCTCACGCCAACCTCAAAACATCTGGCGTTTTCATTTATAAACAGAAAGCAAAACCGAAAATAATCGAATGTACTCCCCGACCATAAAGCGACGGTAGAATATTACtt                                                         >  2:209347/1‑148 (MQ=255)
                                                                                             cACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCAGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGa                                                        <  1:575464/149‑1 (MQ=255)
                                                                                              aCCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCAGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGaa                                                       >  1:323300/1‑148 (MQ=255)
                                                                                               ccAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATa                                                      >  2:142517/1‑149 (MQ=255)
                                                                                                cAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATaa                                                     >  1:969456/1‑149 (MQ=255)
                                                                                                cAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACACCCCGACCATGCAGCAACGGTAGGATATTACTGATaa                                                     >  1:979054/1‑149 (MQ=255)
                                                                                                  gAACCGGTGAATCTCACTCAACAGGATTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAAc                                                   <  2:746710/149‑1 (MQ=255)
                                                                                                     cccgTGCATCTCACACAACAGGCTTCATGTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTAATAAACACAAAGGAAAACCGATAATAAGGGAATGGACGCCCCGACCATCAACCAACGGTACGATATTACTGATACAccat                                                >  2:661740/4‑147 (MQ=255)
                                                                                                        gTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCCTTTTCATTTATAAACAGAAAGGAAAACCGATAAAAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGAAATTACTGATAAACCATCCa                                             >  1:90945/1‑149 (MQ=255)
                                                                                                             tctcACTCAACATGCTTCATTTCTCCTGACGCCAGCCTGAATCCAGCTGGCGTGTTCATTTATAAACAGAAATGAACACCGATAATAATGGAATGGACGCCCCGACCATGAGGCAACGGTAGGATATTACTGATAAACCTTACatttga                                        >  1:1185249/1‑144 (MQ=255)
                                                                                                              ctcactcaACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAg                                       >  2:604416/1‑149 (MQ=255)
                                                                                                               acactcaACATGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAgg                                      <  1:242933/148‑1 (MQ=255)
                                                                                                                    ccacccgCTTCAATTAACCTCCCGCCAGCCAGCATCCCGCTGGCGTTTTCATTTATCAACCCAAACGAACCCCGAAAATAATGGCATGGACGCCCCGACCATGAAGCAACGGTAGGATATTCCTGATAAACCTTCCAGTGGAGGGGCGt                                 <  2:533100/143‑1 (MQ=255)
                                                                                                                    caACAGTCTTCATTTCTCCTGACGACAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGATAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGt                                 <  1:747231/149‑1 (MQ=255)
                                                                                                                     aaCAGGCTTCAATTCTCATGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGtt                                <  2:873023/149‑1 (MQ=255)
                                                                                                                      aCAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAACCAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAGCGGTAGGATATTACTGTTAAACCTTCCTGTGGAGGTGCGttt                               >  2:705750/1‑148 (MQ=255)
                                                                                                                       cAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACTCCCCGACCATGGAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTAt                              >  1:1017996/1‑149 (MQ=255)
                                                                                                                       cAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTAt                              <  1:142517/149‑1 (MQ=255)
                                                                                                                         ggCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATgg                            <  2:571722/149‑1 (MQ=255)
                                                                                                                         ggCTTCATTTCTCCTGACGCCAGCCTGAATACAGCGGGCGTGAGCATTTATAAACAGAAAGGAAAACCGAAAATAATGGAATGGACGCCCCGACCATGAACCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATgg                            <  1:798072/149‑1 (MQ=255)
                                                                                                                            ttCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACTGTAGTATAATACTGATAAACCTTCCACTGGAGGTGCGTTATGGCCg                         >  2:797269/1‑149 (MQ=255)
                                                                                                                                tttCTCCTGACGCCAGCCTTAATACATCTGGAGTTTTCATTTATAAACAGAAAGGAAAACCTATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGtttt                     <  2:393674/149‑1 (MQ=255)
                                                                                                                                   atgaaaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAAc                  >  2:1146621/7‑149 (MQ=255)
                                                                                                                                   atgaaaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATTTTACTGATAAACCTTCCAGGGGAGGTGCGTTATGGCCGTTTTAAc                  >  2:190202/7‑149 (MQ=255)
                                                                                                                                    tcCTGACGCCAGCCTGAATACAGCTGCCGTTTTCATTTTTAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGGAACGGTAGGATATTACTGATAAACCGACCAGTGGACGTGAGTTATGGGCGTTTTTACg                 >  1:484528/1‑149 (MQ=255)
                                                                                                                                       tGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGAtt              >  1:203839/1‑149 (MQ=255)
                                                                                                                                       tGACGCCAGCCTGAATACAGCTGGCGTTTTCATGTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTAAACGAtt              >  1:1168592/1‑149 (MQ=255)
                                                                                                                                       aaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTATCGAtt              >  1:154886/3‑149 (MQ=255)
                                                                                                                                       aaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGAtt              >  2:462953/3‑149 (MQ=255)
                                                                                                                                        gACGCCAGCCTGAATACTGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAACATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTa             <  2:285436/149‑1 (MQ=255)
                                                                                                                                         aCGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAg            >  1:285508/1‑149 (MQ=255)
                                                                                                                                          cGCCATCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAATACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAAGGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGc           <  1:20774/149‑1 (MQ=255)
                                                                                                                                           gCCAGCCTGAATACAGCTGTCGTTTTCATTTATAAACACAAAGGAAAACCGATAATAATGGAATTGACGCCCCGACCATGAAGCAACGGTATGATTTTACTGATAAACCTTCCAGTGGAGGTGCGTTATGTCCGTTTTAACGATTAGCa          <  2:620205/149‑1 (MQ=255)
                                                                                                                                             cAGCCTGAATACAGCTGGCGGTTTCATTTATAAACAGAAAGGACAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCCAGGGTAGGATTTGTCTGATAAACATTCCAGTGGAGGTGCGTTATGGCCGGTTTAACGATTAgaagt        >  2:24190/1‑145 (MQ=255)
                                                                                                                                               gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGa      >  2:922287/1‑149 (MQ=255)
                                                                                                                                               gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGa      <  1:449601/149‑1 (MQ=255)
                                                                                                                                               gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGa      <  1:201137/149‑1 (MQ=255)
                                                                                                                                               gCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAAAGATTAGCATTGa      >  2:922285/1‑149 (MQ=255)
                                                                                                                                                   taaTACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGCTAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTg  <  1:854096/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTG  >  NC_000913/2078153‑2078448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: