Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,354,885 G→A 5.5% A207A (GCG→GCA glpB → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,354,8850GA 5.5% 201.7 / 2.2 72A207A (GCG→GCAglpBanaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base G (31/37);  new base A (2/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGCGTGAACTCGGCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCAT  >  NC_000913/2354738‑2355031
                                                                                                                                                   |                                                                                                                                                  
gatGCGTGAACTCGGCCTAGCCTTTGAAACCGCATAAATAGAGCTGCCGGTACTGGAGGTGCTGCGCAATACCGCCACCGAATTTCTCGCGGTGAATATCGCCCGGTTCCTTGATCATGAAGACTACTGGCCGCTGTTACTTGATgcgc                                                                                                                                                   <  2:703433/147‑1 (MQ=255)
gTTGCGGGAACTCGGCCTTGCCGTTGACACCGCAGAAATAGAGCTGCCGGAACTGGATCCGCGGCGCAATCACGCCCCCGACTTTCGCGCGGGGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATgcgc                                                                                                                                                   <  2:38697/149‑1 (MQ=255)
 gttcGTGAACTCGGCCTTGCCGGTGAATCCGCAGAAATAGAGCTGCCGGAACTGGAGGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGCAGAAATCTGGCCGCTGTTACTTGATGCGCt                                                                                                                                                  <  2:1183844/146‑1 (MQ=255)
   gCGTGAACTCGGCCTTGCCGGTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCTCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTa                                                                                                                                                <  1:800121/149‑1 (MQ=255)
    cGTGAACTCGGCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTAt                                                                                                                                               <  2:369535/149‑1 (MQ=255)
     gTGAACTCGGCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTAtt                                                                                                                                              >  1:472265/1‑149 (MQ=255)
       gaccTCGGCCGGGCCGTTGAAACCGCAGACATAGAGCGGCCGGAACTGGATGTGCTGCGCACTCACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAACACTGGCCGCTGTTACTTGATGCGCTTATTcc                                                                                                                                            <  1:293669/146‑1 (MQ=255)
           tCGGCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTc                                                                                                                                        >  2:211894/1‑149 (MQ=255)
           tCGGCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCATTGATAATCAAGAAAACTGGCCGCTGTTACTTGATGCGATTATTCCTGTc                                                                                                                                        >  2:871245/1‑149 (MQ=255)
             ggCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTGCTTGATAATGAAGAAAACTGCCCGCTGTTACTTGATGCACTTATTCGTGTCGc                                                                                                                                      >  1:512533/1‑149 (MQ=255)
             ggCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGc                                                                                                                                      >  2:1067869/1‑149 (MQ=255)
             ggCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGc                                                                                                                                      <  1:164681/149‑1 (MQ=255)
             ggCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCACTTATTCCTGTCGc                                                                                                                                      >  1:522612/1‑149 (MQ=255)
                 ttGCCGTTGAAACCGCATAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAAt                                                                                                                                  <  2:844803/149‑1 (MQ=255)
                   gCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCTCCattcc                                                                                                                                >  1:101225/1‑145 (MQ=255)
                    ccGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATAcc                                                                                                                               >  1:265097/1‑149 (MQ=255)
                       ttGAAACCGCAGAAATTGAGCTGCCGGAACTGGATGGGCTGCGCAATAAGGCAACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCGTGATAATGAAGATTACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGc                                                                                                                            <  2:668506/149‑1 (MQ=255)
                          aaaCCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGaa                                                                                                                         >  1:20744/1‑149 (MQ=255)
                           aaCCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGGGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGaaa                                                                                                                        <  1:682438/149‑1 (MQ=255)
                            aCCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAAt                                                                                                                       <  2:830831/149‑1 (MQ=255)
                               gCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGAt                                                                                                                    >  1:991563/1‑149 (MQ=255)
                                cAGAAATAGAGCTTCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATc                                                                                                                   <  1:1121228/149‑1 (MQ=255)
                                 aGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATcc                                                                                                                  <  1:858003/149‑1 (MQ=255)
                                     aTAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGAt                                                                                                              <  1:484870/149‑1 (MQ=255)
                                           cTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGc                                                                                                        <  1:685838/149‑1 (MQ=255)
                                             gCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTAGCGCGGTTAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCt                                                                                                      <  1:534060/149‑1 (MQ=255)
                                              ccGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTg                                                                                                     >  2:3024/1‑149 (MQ=255)
                                                   aCTGGATGTGCTGCGCAATAACTCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGAAAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCTAAATGATCCTGATGCCCGCCTGCTTCg                                                                                                <  1:871245/149‑1 (MQ=255)
                                                       gATGTGCTGCGCAATAACGCCCCCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTGACTTGATGCGCTTATTCCTGTCGCCAATAACTGCGAATTGATCCTGATGCCCGCCTGCTTCGGTCt                                                                                            >  1:249377/1‑149 (MQ=255)
                                                            gctgcGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCTGTCTGGCCg                                                                                       >  2:902781/1‑149 (MQ=255)
                                                             ctgcGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGa                                                                                      <  1:469454/149‑1 (MQ=255)
                                                             ctgcGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTATCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGa                                                                                      <  1:174830/149‑1 (MQ=255)
                                                                 ccAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTGTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGAc                                                                                  <  2:336923/148‑1 (MQ=255)
                                                                   aaTAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACaa                                                                                >  1:324766/1‑149 (MQ=255)
                                                                         gCCACCGAATTTCGCGCGGTGAATATCGCCCGGTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACtgtg                                                                          <  1:27048/149‑1 (MQ=255)
                                                                            aCCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTACGAAATGATCCAGATGCCCGACTGCTTCGGTCTGGCCGATGACAAACTGTcgcg                                                                       >  2:641155/1‑149 (MQ=255)
                                                                             ccGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCgt                                                                      >  2:44335/1‑149 (MQ=255)
                                                                                 aTTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAAGTGGCGGGTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCgttggt                                                                  <  1:1142018/149‑1 (MQ=255)
                                                                                    tCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCGAATACCTGCGAAAGGATACTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTtga                                                               >  2:949309/1‑149 (MQ=255)
                                                                                     cgcgcgGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTtgaa                                                              <  1:211894/149‑1 (MQ=255)
                                                                                      gcgcgGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTtgaat                                                             <  1:1051240/149‑1 (MQ=255)
                                                                                      gcgcgGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTtgaat                                                             <  1:344974/149‑1 (MQ=255)
                                                                                         cgGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTtgaatgaa                                                          <  1:729148/149‑1 (MQ=255)
                                                                                          ggTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGaaa                                                         <  1:3024/149‑1 (MQ=255)
                                                                                             tactatCGCCCGTTTACTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAAc                                                      <  2:954002/146‑1 (MQ=255)
                                                                                                tatCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTAc                                                   >  2:968057/1‑149 (MQ=255)
                                                                                                     cccGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCGGTTTCCTGTCGCCAATACCTGCGAAATGATCGTGATGCCAGCCTGCTTCGGTCTGGCCGATGCCATACTGTCTCGATGGTTTAATGAAAAACTACGTTGt                                              >  1:764969/1‑149 (MQ=255)
                                                                                                         tttCCTTTATAATGAAGAAAACTCGCCGCTGTTAATTGATGCGCTTATTCCTGTCGCCATTACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCAc                                          <  2:970776/149‑1 (MQ=255)
                                                                                                             cTTGATAATGAAGAAAACTGGCCGGTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCGGATGACAAACTGTGGCGTTGGTTGAATGAAATACTACCTTGTTCCGTGAt                                      >  2:1177933/1‑149 (MQ=255)
                                                                                                             cTTGATAATGAAGAAAACTGGCCGCTGTTACTTCATGCGCTTATTCATGTCGCCAATACCTGAGAAATGATCCTGATGCCCGCAAGCTTCGGTCTGGCCGATGCCAACCTGTGGCGGTGGTTGAATCAAAGACTACCTTGTTCACTGAt                                      >  1:59263/1‑149 (MQ=255)
                                                                                                             cTTGATAATGAAGAAAACTGGACGCTGTTACTTGATGCGCTTATTCGTGTCGCCAATACCTGCGAAATGCTCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACTAACTGTGGCGTTGGTTTAATGAAAAACTACCTTGTTCACTGAt                                      <  1:1058500/149‑1 (MQ=255)
                                                                                                              ttGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGATATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATg                                     <  1:255063/149‑1 (MQ=255)
                                                                                                              ttGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACACACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACAGATg                                     >  2:124359/1‑149 (MQ=255)
                                                                                                              ttGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATg                                     >  1:568663/1‑149 (MQ=255)
                                                                                                               tGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGc                                    <  2:724567/149‑1 (MQ=255)
                                                                                                                gATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTCTCGCCAATACCTGCGAAATGATCATGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTCTGGCTTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCt                                   >  2:468798/1‑149 (MQ=255)
                                                                                                                   aaTGAATAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCAAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCtttt                                <  2:101225/149‑1 (MQ=255)
                                                                                                                   aaTGAAGAACACTGGCCGCTGTTACTTGATGCGCTTAATCCTGTCGCCATTACCTGCTAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCtttt                                <  2:253924/149‑1 (MQ=255)
                                                                                                                   aaTGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGCTCCTGATGCCCGCCTGCTTCGGACTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCtatt                                >  1:860158/1‑149 (MQ=255)
                                                                                                                   aaTGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCtttt                                >  1:451540/1‑149 (MQ=255)
                                                                                                                   aaTGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCACGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCtttt                                >  1:151985/1‑149 (MQ=255)
                                                                                                                    aTGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTg                               <  1:1067869/149‑1 (MQ=255)
                                                                                                                     tgatgcaaaCTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGAACCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGc                              <  1:952897/143‑1 (MQ=255)
                                                                                                                            aaCTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCt                       >  2:966457/1‑149 (MQ=255)
                                                                                                                             aCTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTGGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTg                      <  1:1087672/149‑1 (MQ=255)
                                                                                                                               tGGCCGCTGTTACTTTATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGCTCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTgcc                    <  2:494883/149‑1 (MQ=255)
                                                                                                                                 gCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTgccgc                  >  2:1063322/1‑149 (MQ=255)
                                                                                                                                     cTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTc              >  1:684269/1‑149 (MQ=255)
                                                                                                                                       gTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCg            >  1:502115/1‑149 (MQ=255)
                                                                                                                                           cTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGCAAACCTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCt        >  2:1036203/1‑149 (MQ=255)
                                                                                                                                           cTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCt        <  2:265097/149‑1 (MQ=255)
                                                                                                                                            ttgttgCACTTATTCCTGTCGCCACTACCTGCGAAATGATCCAGCTGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGCTGCTTTTGCCAACGCTGCCGCCTTCCGTGCTg       <  2:512533/149‑1 (MQ=255)
                                                                                                                                            ttGATGCACTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGTCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTg       <  2:522612/149‑1 (MQ=255)
                                                                                                                                             tGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTgg      >  1:548631/1‑149 (MQ=255)
                                                                                                                                                 gcgcTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCAt  <  1:968057/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTGCGTGAACTCGGCCTTGCCGTTGAAACCGCAGAAATAGAGCTGCCGGAACTGGATGTGCTGCGCAATAACGCCACCGAATTTCGCGCGGTGAATATCGCCCGTTTCCTTGATAATGAAGAAAACTGGCCGCTGTTACTTGATGCGCTTATTCCTGTCGCCAATACCTGCGAAATGATCCTGATGCCCGCCTGCTTCGGTCTGGCCGATGACAAACTGTGGCGTTGGTTGAATGAAAAACTACCTTGTTCACTGATGCTTTTGCCAACGCTGCCGCCTTCCGTGCTGGGCAT  >  NC_000913/2354738‑2355031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: