Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,373,177 G→A 8.2% G96S (GGC→AGC)  arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,373,1770GA 8.2% 214.1 / 8.4 85G96S (GGC→AGC) arnFundecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
Reads supporting (aligned to +/- strand):  ref base G (43/34);  new base A (4/3);  total (48/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-01

GCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATA  >  NC_000913/2373030‑2373325
                                                                                                                                                   |                                                                                                                                                    
gCCCGGATACTGTTACTCGGGCTTCTTGGTTATATGCTTTCCGTTTTTTGTTGGTATAAAAAGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCgg                                                                                                                                                     <  2:1003937/149‑1 (MQ=255)
  ccGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCACAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCt                                                                                                                                                   >  2:721203/1‑149 (MQ=255)
  ccGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCt                                                                                                                                                   >  1:124910/1‑149 (MQ=255)
       tACTGTTACTCGGGCTGCTGGGTTATCTGCTATCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGATGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGaa                                                                                                                                              <  2:1074614/149‑1 (MQ=255)
        aCTGTTACTGGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACATCCCGGCTGGGAAg                                                                                                                                             <  2:862878/149‑1 (MQ=255)
        aCTGTTACTCGGGCTGCTAGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAg                                                                                                                                             <  2:388335/149‑1 (MQ=255)
          tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAggg                                                                                                                                           >  1:493055/1‑149 (MQ=255)
          tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgta                                                                                                                                           <  1:497935/149‑3 (MQ=255)
          tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgta                                                                                                                                           <  1:493547/149‑3 (MQ=255)
          tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTAAATGGTACTTCCCGGCTGGGAAggg                                                                                                                                           >  1:748809/1‑149 (MQ=255)
          tGTTACTCGGGCTGCTGGGTGATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgta                                                                                                                                           <  1:488890/149‑3 (MQ=255)
           gTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAggta                                                                                                                                          >  2:449024/1‑147 (MQ=255)
            taccTCGGGCTGCTCGGTTATCTGCTTTCCGGTTTTTGTTGGTATAAAACGCTTCATAAACTTCCGCTAAGCAAAGCCTCTGCCTTGCTCAGAATGAGTTATGTCCTGGTATGGATGGCTTCAATGGTACTTCCCGGCTGGGAAGGGAc                                                                                                                                         <  2:933956/146‑1 (MQ=255)
              aCTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACtt                                                                                                                                       >  1:340300/1‑149 (MQ=255)
                tCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGATATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACtttt                                                                                                                                     >  1:366178/1‑149 (MQ=255)
                 cGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCTGGGAAGGGACttttt                                                                                                                                    >  2:568264/1‑149 (MQ=255)
                  gggCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTc                                                                                                                                   >  1:23521/1‑149 (MQ=255)
                   ggCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTTCTTTTATTTATGGCGTCACTGGGACGGCCCAACGACGAAGGGACTTTTTCg                                                                                                                                  >  2:1186185/1‑149 (MQ=255)
                   ggCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCg                                                                                                                                  >  2:828249/1‑149 (MQ=255)
                        ctgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTACTCGTATGGATTGCTACAATGCTACTTCCCGGCTGCGAATGGACTTTTTCGCTGaa                                                                                                                             >  1:765176/1‑149 (MQ=255)
                             ttATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCac                                                                                                                        <  1:347511/149‑1 (MQ=255)
                                 cTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCTGTATGAGTTATGTGCGGGGTTGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCactcct                                                                                                                    >  1:1066288/1‑149 (MQ=255)
                                     tttCCGTTTTTTGTTGGTATATAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGa                                                                                                                <  2:608026/149‑1 (MQ=255)
                                      ttCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGATGAAAGCACTACTGCGAg                                                                                                               >  2:907449/1‑149 (MQ=255)
                                          gTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGGCTTTTTCGCTGAAATCACTACTGGGAGTAGc                                                                                                           >  1:1034965/1‑149 (MQ=255)
                                          gTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGc                                                                                                           >  2:862229/1‑149 (MQ=255)
                                          gTTTTTTGTTGGGATAAACCGCTTCATAAACTTGCGCTCAGCAAAGCCTATGCCTTCCGCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGc                                                                                                           <  2:770678/149‑1 (MQ=255)
                                           ttttttGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGca                                                                                                          >  1:456564/1‑148 (MQ=255)
                                               ttgttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGta                                                                                                      <  2:451561/149‑1 (MQ=255)
                                               ttgttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGta                                                                                                      <  1:891567/149‑1 (MQ=255)
                                                 gttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCGCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGtatt                                                                                                    <  2:1142631/149‑1 (MQ=255)
                                                   tgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATTGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGtattat                                                                                                  <  2:778980/149‑1 (MQ=255)
                                                      tataAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAg                                                                                               >  2:534851/1‑149 (MQ=255)
                                                        taAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCg                                                                                             >  2:703761/1‑149 (MQ=255)
                                                         aaaaCGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCgg                                                                                            <  2:1018378/149‑1 (MQ=255)
                                                         aaaaCGCTTCATAAAATTGCGCTAAGCAAAGCCTATGACTTGCTCAGTATGAGTTAAGTGCTGGAAAGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCgg                                                                                            >  2:118333/1‑149 (MQ=255)
                                                            aCGCTTCATAAACTTGCGCTATGCAAAGCCTATGCCTTGCTCAGTAAGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGtt                                                                                         >  2:10316/1‑149 (MQ=255)
                                                              gCTTCATAAACTTGCGCTAGGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTTAAAGCACTTCTGGGAGTAGCCTGTATTATGAGCGGGTTGa                                                                                       >  1:486845/1‑149 (MQ=255)
                                                              gCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGCACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCGGTATTAGGAGCGGGTTGa                                                                                       >  1:1074640/1‑149 (MQ=255)
                                                               cTTCATAACCTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGAt                                                                                      <  2:612936/149‑1 (MQ=255)
                                                               cTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGAt                                                                                      >  2:745175/1‑149 (MQ=255)
                                                                   aTAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTg                                                                                  >  1:646088/1‑149 (MQ=255)
                                                                     aaaCTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGAt                                                                                >  1:895915/1‑149 (MQ=255)
                                                                       aCTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGAttt                                                                              >  2:810399/1‑149 (MQ=255)
                                                                          tGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTAATTTTcc                                                                           <  2:561544/149‑1 (MQ=255)
                                                                             gcTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGc                                                                        >  1:205247/1‑149 (MQ=255)
                                                                                aaGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCTGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCa                                                                     >  2:311979/1‑149 (MQ=255)
                                                                                 aGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCAc                                                                    >  1:1000433/1‑149 (MQ=255)
                                                                                 aGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTAGTATGAGCGGGTTGATGCTGATTTTCCTGCCCAc                                                                    >  2:1138570/1‑149 (MQ=255)
                                                                                  gCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGTGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACg                                                                   >  1:747054/1‑149 (MQ=255)
                                                                                     aaGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGAAATTTTCGCTGAAAGCACTACTGGGAGTACCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGACCACGACa                                                                >  1:929588/1‑149 (MQ=255)
                                                                                       gCCTAAGCCTTGCTCAGTATGCGTAATGTGCTGGTATGGATTGCTTCAATGGGACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACaaa                                                              <  1:662016/149‑1 (MQ=255)
                                                                                         cTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaac                                                            >  1:806076/1‑149 (MQ=255)
                                                                                                ttGCGCAGTATGCGTTATGTGCTGGTAGGCATACCATCAATGGTACTTCCCGGCGGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCGGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTa                                                     <  1:821790/149‑1 (MQ=255)
                                                                                                   cTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACTTTACTg                                                  >  1:343017/1‑149 (MQ=255)
                                                                                                    ggtgTATGAGTTATGTGCTTGTATTGATTGCTTCATTGTTACTTCGCGGCTGGGATGGGACTTTTTCGCGGAATGCACTACTGGGAGTAGCCTGTATTATGAGCTGGTGGATGCTGATTTTCCTGCCCACGACAATACATCGTTACTGa                                                 <  2:765176/146‑1 (MQ=255)
                                                                                                     cAGTATGAGTTATGTGCTGGGATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAg                                                <  2:955667/149‑1 (MQ=255)
                                                                                                       gTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGTCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGCGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGtt                                              <  1:679431/149‑1 (MQ=255)
                                                                                                          tGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTc                                           <  2:895915/149‑1 (MQ=255)
                                                                                                           taGTTATGTGCTGGTATGGATTGCTTCAATGGTACATCCCGGCTGGGAAGGGACTTTATCGCTGAAAGCACTACTGGGAGTAGCCTGTCTTATGAGCGTGTTGATGCTGATTTTCCTGCCCACGACAAAACTACGTTACTGAGTTTTcc                                          <  2:913175/148‑1 (MQ=255)
                                                                                                           gAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTcc                                          >  1:326980/1‑149 (MQ=255)
                                                                                                           gAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTcc                                          >  1:292177/1‑149 (MQ=255)
                                                                                                            aGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTccc                                         <  2:23521/149‑1 (MQ=255)
                                                                                                             gTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTAccat                                        >  2:591569/1‑147 (MQ=255)
                                                                                                               tATGTGCTGGTATGGATTGCTTCAATGGGACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGc                                      <  1:365088/149‑1 (MQ=255)
                                                                                                                      tGGTATGTATTGCTTCAATGGTACTTCCCGGCTGGGTTGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTTTATTATGAGAGTGTTGATTCTGATTTTCCTGCCCACGACAAAAAAACGTTACTGAGTTTTCCCTTCCACTTTa                               <  2:646088/149‑1 (MQ=255)
                                                                                                                        gTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTaca                             >  2:1099478/1‑149 (MQ=255)
                                                                                                                        gTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTaca                             <  2:124910/149‑1 (MQ=255)
                                                                                                                             gATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGcc                        <  1:449024/149‑1 (MQ=255)
                                                                                                                              aTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACATTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCGCGACAAAACAAGGTTACTGAGTTTTCCCTGCCACTTTACACTGCCg                       >  1:75105/1‑149 (MQ=255)
                                                                                                                                tGCTTCATTGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTTAAAGCACTTCTGGGAGTTGCCTGTATTATGAGAGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGtt                     <  2:1074640/149‑1 (MQ=255)
                                                                                                                                  catCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTCTGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTcc                   <  2:217225/147‑1 (MQ=255)
                                                                                                                                   ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                  >  2:29663/1‑149 (MQ=255)
                                                                                                                                   ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                  <  1:703761/149‑1 (MQ=255)
                                                                                                                                   ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGATGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                  >  1:655929/1‑149 (MQ=255)
                                                                                                                                   ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTAGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTAACACTGCCGATcca                  >  1:234342/1‑148 (MQ=255)
                                                                                                                                     cAATTGTACTTCCCGGCTGGTAAGGGACTTTTTCGCTCAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGTTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCAc                <  2:234342/149‑1 (MQ=255)
                                                                                                                                     cAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCAc                <  2:493055/149‑1 (MQ=255)
                                                                                                                                         gtcccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACCCTGCCGTTCCCACTCAt            >  2:260838/5‑149 (MQ=255)
                                                                                                                                             cTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCggg        >  1:512785/1‑149 (MQ=255)
                                                                                                                                              ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGTTTGATGCTGATTTTCCTGCCCACGACATAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg       <  1:919794/149‑1 (MQ=255)
                                                                                                                                              ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg       >  2:709079/1‑149 (MQ=255)
                                                                                                                                              ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg       <  1:461605/149‑1 (MQ=255)
                                                                                                                                              ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg       >  1:473508/1‑149 (MQ=255)
                                                                                                                                              ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTAAACTGCCGTTCCCACTCATCgggg       >  1:1040313/1‑149 (MQ=255)
                                                                                                                                               tCCCGGCTGGGAAGTGACTTTTTAGCTGAAAGCACTTCTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGACGTTCCCACTCATCGGGGc      <  2:366178/149‑1 (MQ=255)
                                                                                                                                                   ggCTGGGAAGGGACTTTTTCGCTGAAATCACTAGTGGGAGTAGCATGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCCCTTTACCCTGCCGTGCCCACTCAACGTGGCtata  >  2:963897/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATA  >  NC_000913/2373030‑2373325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: