Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,373,180 T→C 5.9% W97R (TGG→CGG)  arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,373,1800TC 5.9% 217.4 / 5.1 85W97R (TGG→CGG) arnFundecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
Reads supporting (aligned to +/- strand):  ref base T (44/34);  new base C (2/3);  total (47/38)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-02

CCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATAA  >  NC_000913/2373032‑2373326
                                                                                                                                                    |                                                                                                                                                  
ccGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCACAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCt                                                                                                                                                    >  2:721203/1‑149 (MQ=255)
ccGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCt                                                                                                                                                    >  1:124910/1‑149 (MQ=255)
     tACTGTTACTCGGGCTGCTGGGTTATCTGCTATCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGATGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGaa                                                                                                                                               <  2:1074614/149‑1 (MQ=255)
      aCTGTTACTGGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACATCCCGGCTGGGAAg                                                                                                                                              <  2:862878/149‑1 (MQ=255)
      aCTGTTACTCGGGCTGCTAGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAg                                                                                                                                              <  2:388335/149‑1 (MQ=255)
        tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAggg                                                                                                                                            >  1:493055/1‑149 (MQ=255)
        tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgta                                                                                                                                            <  1:493547/149‑3 (MQ=255)
        tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgta                                                                                                                                            <  1:497935/149‑3 (MQ=255)
        tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTAAATGGTACTTCCCGGCTGGGAAggg                                                                                                                                            >  1:748809/1‑149 (MQ=255)
        tGTTACTCGGGCTGCTGGGTGATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgta                                                                                                                                            <  1:488890/149‑3 (MQ=255)
         gTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAggta                                                                                                                                           >  2:449024/1‑147 (MQ=255)
          taccTCGGGCTGCTCGGTTATCTGCTTTCCGGTTTTTGTTGGTATAAAACGCTTCATAAACTTCCGCTAAGCAAAGCCTCTGCCTTGCTCAGAATGAGTTATGTCCTGGTATGGATGGCTTCAATGGTACTTCCCGGCTGGGAAGGGAc                                                                                                                                          <  2:933956/146‑1 (MQ=255)
            aCTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACtt                                                                                                                                        >  1:340300/1‑149 (MQ=255)
              tCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGATATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACtttt                                                                                                                                      >  1:366178/1‑149 (MQ=255)
               cGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCTGGGAAGGGACttttt                                                                                                                                     >  2:568264/1‑149 (MQ=255)
                gggCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTc                                                                                                                                    >  1:23521/1‑149 (MQ=255)
                 ggCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTTCTTTTATTTATGGCGTCACTGGGACGGCCCAACGACGAAGGGACTTTTTCg                                                                                                                                   >  2:1186185/1‑149 (MQ=255)
                 ggCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCg                                                                                                                                   >  2:828249/1‑149 (MQ=255)
                      ctgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTACTCGTATGGATTGCTACAATGCTACTTCCCGGCTGCGAATGGACTTTTTCGCTGaa                                                                                                                              >  1:765176/1‑149 (MQ=255)
                           ttATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCac                                                                                                                         <  1:347511/149‑1 (MQ=255)
                               cTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCTGTATGAGTTATGTGCGGGGTTGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCactcct                                                                                                                     >  1:1066288/1‑149 (MQ=255)
                                   tttCCGTTTTTTGTTGGTATATAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGa                                                                                                                 <  2:608026/149‑1 (MQ=255)
                                    ttCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGATGAAAGCACTACTGCGAg                                                                                                                >  2:907449/1‑149 (MQ=255)
                                        gTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGGCTTTTTCGCTGAAATCACTACTGGGAGTAGc                                                                                                            >  1:1034965/1‑149 (MQ=255)
                                        gTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGc                                                                                                            >  2:862229/1‑149 (MQ=255)
                                        gTTTTTTGTTGGGATAAACCGCTTCATAAACTTGCGCTCAGCAAAGCCTATGCCTTCCGCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGc                                                                                                            <  2:770678/149‑1 (MQ=255)
                                         ttttttGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGca                                                                                                           >  1:456564/1‑148 (MQ=255)
                                             ttgttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGta                                                                                                       <  2:451561/149‑1 (MQ=255)
                                             ttgttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGta                                                                                                       <  1:891567/149‑1 (MQ=255)
                                               gttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCGCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGtatt                                                                                                     <  2:1142631/149‑1 (MQ=255)
                                                 tgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATTGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGtattat                                                                                                   <  2:778980/149‑1 (MQ=255)
                                                    tataAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAg                                                                                                >  2:534851/1‑149 (MQ=255)
                                                      taAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCg                                                                                              >  2:703761/1‑149 (MQ=255)
                                                       aaaaCGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCgg                                                                                             <  2:1018378/149‑1 (MQ=255)
                                                       aaaaCGCTTCATAAAATTGCGCTAAGCAAAGCCTATGACTTGCTCAGTATGAGTTAAGTGCTGGAAAGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCgg                                                                                             >  2:118333/1‑149 (MQ=255)
                                                          aCGCTTCATAAACTTGCGCTATGCAAAGCCTATGCCTTGCTCAGTAAGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGtt                                                                                          >  2:10316/1‑149 (MQ=255)
                                                            gCTTCATAAACTTGCGCTAGGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTTAAAGCACTTCTGGGAGTAGCCTGTATTATGAGCGGGTTGa                                                                                        >  1:486845/1‑149 (MQ=255)
                                                            gCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGCACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCGGTATTAGGAGCGGGTTGa                                                                                        >  1:1074640/1‑149 (MQ=255)
                                                             cTTCATAACCTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGAt                                                                                       <  2:612936/149‑1 (MQ=255)
                                                             cTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGAt                                                                                       >  2:745175/1‑149 (MQ=255)
                                                                 aTAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTg                                                                                   >  1:646088/1‑149 (MQ=255)
                                                                   aaaCTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGAt                                                                                 >  1:895915/1‑149 (MQ=255)
                                                                     aCTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGAttt                                                                               >  2:810399/1‑149 (MQ=255)
                                                                        tGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTAATTTTcc                                                                            <  2:561544/149‑1 (MQ=255)
                                                                           gcTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGc                                                                         >  1:205247/1‑149 (MQ=255)
                                                                              aaGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCTGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCa                                                                      >  2:311979/1‑149 (MQ=255)
                                                                               aGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCAc                                                                     >  1:1000433/1‑149 (MQ=255)
                                                                               aGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTAGTATGAGCGGGTTGATGCTGATTTTCCTGCCCAc                                                                     >  2:1138570/1‑149 (MQ=255)
                                                                                gCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGTGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACg                                                                    >  1:747054/1‑149 (MQ=255)
                                                                                   aaGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGAAATTTTCGCTGAAAGCACTACTGGGAGTACCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGACCACGACa                                                                 >  1:929588/1‑149 (MQ=255)
                                                                                     gCCTAAGCCTTGCTCAGTATGCGTAATGTGCTGGTATGGATTGCTTCAATGGGACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACaaa                                                               <  1:662016/149‑1 (MQ=255)
                                                                                       cTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaac                                                             >  1:806076/1‑149 (MQ=255)
                                                                                              ttGCGCAGTATGCGTTATGTGCTGGTAGGCATACCATCAATGGTACTTCCCGGCGGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCGGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTa                                                      <  1:821790/149‑1 (MQ=255)
                                                                                                 cTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACTTTACTg                                                   >  1:343017/1‑149 (MQ=255)
                                                                                                  ggtgTATGAGTTATGTGCTTGTATTGATTGCTTCATTGTTACTTCGCGGCTGGGATGGGACTTTTTCGCGGAATGCACTACTGGGAGTAGCCTGTATTATGAGCTGGTGGATGCTGATTTTCCTGCCCACGACAATACATCGTTACTGa                                                  <  2:765176/146‑1 (MQ=255)
                                                                                                   cAGTATGAGTTATGTGCTGGGATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAg                                                 <  2:955667/149‑1 (MQ=255)
                                                                                                     gTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGTCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGCGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGtt                                               <  1:679431/149‑1 (MQ=255)
                                                                                                        tGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTc                                            <  2:895915/149‑1 (MQ=255)
                                                                                                         taGTTATGTGCTGGTATGGATTGCTTCAATGGTACATCCCGGCTGGGAAGGGACTTTATCGCTGAAAGCACTACTGGGAGTAGCCTGTCTTATGAGCGTGTTGATGCTGATTTTCCTGCCCACGACAAAACTACGTTACTGAGTTTTcc                                           <  2:913175/148‑1 (MQ=255)
                                                                                                         gAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTcc                                           >  1:292177/1‑149 (MQ=255)
                                                                                                         gAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTcc                                           >  1:326980/1‑149 (MQ=255)
                                                                                                          aGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTccc                                          <  2:23521/149‑1 (MQ=255)
                                                                                                           gTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTAccat                                         >  2:591569/1‑147 (MQ=255)
                                                                                                             tATGTGCTGGTATGGATTGCTTCAATGGGACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGc                                       <  1:365088/149‑1 (MQ=255)
                                                                                                                    tGGTATGTATTGCTTCAATGGTACTTCCCGGCTGGGTTGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTTTATTATGAGAGTGTTGATTCTGATTTTCCTGCCCACGACAAAAAAACGTTACTGAGTTTTCCCTTCCACTTTa                                <  2:646088/149‑1 (MQ=255)
                                                                                                                      gTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTaca                              >  2:1099478/1‑149 (MQ=255)
                                                                                                                      gTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTaca                              <  2:124910/149‑1 (MQ=255)
                                                                                                                           gATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGcc                         <  1:449024/149‑1 (MQ=255)
                                                                                                                            aTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACATTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCGCGACAAAACAAGGTTACTGAGTTTTCCCTGCCACTTTACACTGCCg                        >  1:75105/1‑149 (MQ=255)
                                                                                                                              tGCTTCATTGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTTAAAGCACTTCTGGGAGTTGCCTGTATTATGAGAGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGtt                      <  2:1074640/149‑1 (MQ=255)
                                                                                                                                catCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTCTGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTcc                    <  2:217225/147‑1 (MQ=255)
                                                                                                                                 ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                   >  2:29663/1‑149 (MQ=255)
                                                                                                                                 ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                   <  1:703761/149‑1 (MQ=255)
                                                                                                                                 ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGATGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                   >  1:655929/1‑149 (MQ=255)
                                                                                                                                 ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTAGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTAACACTGCCGATcca                   >  1:234342/1‑148 (MQ=255)
                                                                                                                                   cAATTGTACTTCCCGGCTGGTAAGGGACTTTTTCGCTCAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGTTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCAc                 <  2:234342/149‑1 (MQ=255)
                                                                                                                                   cAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCAc                 <  2:493055/149‑1 (MQ=255)
                                                                                                                                       gtcccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACCCTGCCGTTCCCACTCAt             >  2:260838/5‑149 (MQ=255)
                                                                                                                                           cTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCggg         >  1:512785/1‑149 (MQ=255)
                                                                                                                                            ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGTTTGATGCTGATTTTCCTGCCCACGACATAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg        <  1:919794/149‑1 (MQ=255)
                                                                                                                                            ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg        >  2:709079/1‑149 (MQ=255)
                                                                                                                                            ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg        <  1:461605/149‑1 (MQ=255)
                                                                                                                                            ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg        >  1:473508/1‑149 (MQ=255)
                                                                                                                                            ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTAAACTGCCGTTCCCACTCATCgggg        >  1:1040313/1‑149 (MQ=255)
                                                                                                                                             tCCCGGCTGGGAAGTGACTTTTTAGCTGAAAGCACTTCTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGACGTTCCCACTCATCGGGGc       <  2:366178/149‑1 (MQ=255)
                                                                                                                                                 ggCTGGGAAGGGACTTTTTCGCTGAAATCACTAGTGGGAGTAGCATGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCCCTTTACCCTGCCGTGCCCACTCAACGTGGCtata   >  2:963897/1‑149 (MQ=255)
                                                                                                                                                  gCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATaa  <  1:862229/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATAA  >  NC_000913/2373032‑2373326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: