Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,399,876 G→A 5.8% T97I (ACC→ATC)  nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,399,8760GA 5.8% 193.1 / 2.5 69T97I (ACC→ATC) nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base G (35/30);  new base A (2/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

GTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCAC  >  NC_000913/2399728‑2400023
                                                                                                                                                    |                                                                                                                                                   
gTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCCCATACCGGACAGTCGTGCGGGtggttgg                                                                                                                                                     >  1:899849/1‑149 (MQ=255)
    gagaTGAATGGCCCCAAATCCTCATTACGGTGGGAACGTTTGGTGAACCGTTAGCCACGGAAGCTGTGACCGCTCATCACAGTCATAACCTGAACATGGCAGTTACCGCCCGCTTCACATCCCGGCCCGTCCTGCGGTTCGTTGGtcat                                                                                                                                                 >  2:674846/1‑149 (MQ=255)
       aTGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAATCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCTCATACCGGACAGTCGTGCGGGTGGTTGGTCATCaa                                                                                                                                              <  2:319423/149‑1 (MQ=255)
       aTGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCATCTTCACATACCGGACAGTCGTGCGGTTGTTTGGTCATCaa                                                                                                                                              >  2:699830/1‑149 (MQ=255)
          aaTGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAccc                                                                                                                                           >  2:1045827/1‑148 (MQ=255)
             ggCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTc                                                                                                                                        >  1:45090/1‑149 (MQ=255)
                cccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGGGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTCACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGAc                                                                                                                                     <  2:689042/149‑1 (MQ=255)
                cccAAATCCTGAATACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCAGGTCATCACAGTCATATACTGAAGATGGCAGTTACCGCCCTCTTCACAAACAGGACAGTCGTCCGGCTGCTTGGTCATCTCCCCCTCGAc                                                                                                                                     >  2:519434/1‑149 (MQ=255)
                 ccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGGCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGAGTGGTTGATGACCAACCACCCgcac                                                                                                                                    <  1:558962/149‑4 (MQ=255)
                 ccAAATCCTGATTACGGGGGGTACGGTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGGCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGATGACCAACCACCCgcac                                                                                                                                    <  2:1004993/149‑4 (MQ=255)
                  cAAATCCTGATAACGGTGGGTACGTTTGGTCAAACGGAAGCGACTGAAGCTGTGTCCGGTCATCACACTCATATACTGAAGATGGCAGATACCGCCCACTTCACATACCGCACACTCGTGCGCGATCTTGGTCATCAACCACTCGAccc                                                                                                                                   >  2:600173/1‑148 (MQ=255)
                    aaTCCTGATTTCGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACg                                                                                                                                 <  2:943314/149‑1 (MQ=255)
                    aaTCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACg                                                                                                                                 <  2:938786/149‑1 (MQ=255)
                        cTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGGTGGTCATCAACCACTCGACCACGCttt                                                                                                                             <  2:621418/149‑1 (MQ=255)
                         tGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTc                                                                                                                            <  1:248833/149‑1 (MQ=255)
                         tGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTGCACATACAGGACAGTCGTGCGGGTGGATGGACATCAACCCCTCGACCCCGCTTTc                                                                                                                            >  2:1105133/1‑149 (MQ=255)
                               cGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGaa                                                                                                                      <  1:485315/149‑1 (MQ=255)
                                ggTGGGTAAGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAAc                                                                                                                     <  2:828545/149‑1 (MQ=255)
                                  tGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTg                                                                                                                   >  1:743929/1‑149 (MQ=255)
                                  ggggTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTg                                                                                                                   <  1:1075843/148‑1 (MQ=255)
                                    ggTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATACTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGtt                                                                                                                 >  1:967388/1‑149 (MQ=255)
                                      tACGTTTGGTGAAACGGTAGCGACGGCAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTc                                                                                                               <  1:1149238/149‑1 (MQ=255)
                                        cgctctctGAAACGGTAGCGACGGAAGCTGTCGCCTGTCCTCACAGTCATCTCCTGCAGAAGGCAGTTACCGCCCTCTTCACATACCGTCCAGGCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGc                                                                                                             <  2:820685/142‑1 (MQ=255)
                                        cGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGGCTTCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGc                                                                                                             <  1:192462/149‑1 (MQ=255)
                                         gTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGct                                                                                                            >  2:275438/1‑149 (MQ=255)
                                               tGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcg                                                                                                      >  2:406830/1‑149 (MQ=255)
                                                gAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcgt                                                                                                     <  2:1141868/149‑1 (MQ=255)
                                                gAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCACTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcgt                                                                                                     >  1:1033735/1‑149 (MQ=255)
                                                gAAACGGTAGCGACGAAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcgt                                                                                                     <  1:306865/149‑1 (MQ=255)
                                                  accGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTAACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGCCCACGCTTTCACGGAACTGTTTCGCTTCTtcgtcg                                                                                                   >  1:933136/3‑149 (MQ=255)
                                                  aaCGGTAGCGACGGAAGCTGTGTACTGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACCTACCGGACAGTCGTGCGTGTAGTTGGTCATCCAACACTCGCCCAATCTTTCAACGAACTGTTTCCCTTCTgcgacg                                                                                                   >  2:1165326/1‑149 (MQ=255)
                                                       tAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAAt                                                                                              <  1:730890/149‑1 (MQ=255)
                                                          cGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCGTCTTCGTCGTCAATGGa                                                                                           >  1:191833/1‑149 (MQ=255)
                                                                aaGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATaaa                                                                                     <  2:899849/149‑1 (MQ=255)
                                                                 aGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAg                                                                                    <  1:1045827/149‑1 (MQ=255)
                                                                 aGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAg                                                                                    <  1:1158362/149‑1 (MQ=255)
                                                                  gCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAgg                                                                                   >  1:624734/1‑149 (MQ=255)
                                                                  cctgggcccGGTCCTCACACTCATACCCTGAAGCTGCCAGCACCCGCCCTCTTCACATCCCCGACAGGCGTGCGGGGGGTAGGCCATCACCCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTGCGTCGTCAATGGAAATAAAgg                                                                                   <  2:868105/142‑1 (MQ=255)
                                                                      tgtCCGGTCATCCCAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACCTACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCAAGGAACTGTTGCGCTTATTAGTCGTCAATGGAAATAAAGGTgac                                                                               >  1:908069/1‑147 (MQ=255)
                                                                      tgtCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACGGCCCTCATCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGTACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGcc                                                                               <  1:600173/149‑1 (MQ=255)
                                                                             tcatcaCAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATCCCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGaa                                                                        >  2:1058009/1‑149 (MQ=255)
                                                                              catcaCAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCCCTCGACCACGCTTTCACGGAACGGTTTCGCTTCTTCGGCGGCAATGGAACTAAAGGTGCCCTCGGaac                                                                       >  1:781761/1‑148 (MQ=255)
                                                                                    aGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTAGACCCCGCTTTCACGGACCTGTTTCGCTTCTTCGGCGTCAATGGCAATAAAGGGGCCATCGGAAGCCgggg                                                                 >  2:802180/1‑147 (MQ=255)
                                                                                      tCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCAGTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTc                                                               <  1:956912/149‑1 (MQ=255)
                                                                                      tCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCATTGGAAATAAAGGTGCCATCGGAAGCCGGTGTc                                                               >  1:318989/1‑149 (MQ=255)
                                                                                          atCCTGAAGATGGCAGTTACCGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATAc                                                           <  1:344352/149‑1 (MQ=255)
                                                                                            tgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAg                                                         >  1:626863/3‑149 (MQ=255)
                                                                                            ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAg                                                         <  2:923320/149‑1 (MQ=255)
                                                                                             cTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAGCCACTCGACCCCGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAAGGGAAATTAAGGTGCCAGCGGAAGCCGGTGTGATACAgg                                                        >  1:148452/1‑149 (MQ=255)
                                                                                              tGAAGATGGCAGTTACCGCCCTCTTCACATAACGGACAGTCGTGCGGGTGGTTGGTCATCCACCACTCGACCACGCGTTCACGGAACGGGTTCGCTTATTCGTCTTCAATGGAAATAAAGGTGCGATCGGAAGCCGGTGTCATACAGGa                                                       >  2:867727/1‑149 (MQ=255)
                                                                                              tGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGa                                                       >  1:785006/1‑149 (MQ=255)
                                                                                                 aGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACAt                                                    <  1:515705/149‑1 (MQ=255)
                                                                                                     ggCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAcc                                                >  2:917281/1‑149 (MQ=255)
                                                                                                       cAGTGACCGCCCTCTTCACATACCGGACAGTCGTGCGGGGGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACATGACATCACCAg                                              <  2:839673/149‑1 (MQ=255)
                                                                                                             ccGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGAcc                                        >  1:574956/1‑149 (MQ=255)
                                                                                                             ccGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACACCGCTTTCACGGAACTGTTTCGATTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGAcc                                        >  2:18057/1‑149 (MQ=255)
                                                                                                              cGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCa                                       >  1:320514/1‑149 (MQ=255)
                                                                                                                cccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTATCACGGAACTGTTTCGCTTCTTCGTCGTCAAAGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGCCATCACCAGGCGACCAcg                                     >  2:358363/1‑149 (MQ=255)
                                                                                                                 ccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcgc                                    <  2:967388/149‑1 (MQ=255)
                                                                                                                    ctTCACAAACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCAGGTGTCATACAGGACATCACCAGGCGACCACGCgtg                                 >  2:921056/1‑149 (MQ=255)
                                                                                                                              cGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGt                       <  2:785006/149‑1 (MQ=255)
                                                                                                                                  cAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTg                   <  1:406830/149‑1 (MQ=255)
                                                                                                                                     tCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTa                >  2:47975/1‑149 (MQ=255)
                                                                                                                                     tCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTa                >  1:881972/1‑149 (MQ=255)
                                                                                                                                      tggtcgagtggtTGATGACCAACCACCCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAt               >  1:334008/8‑149 (MQ=255)
                                                                                                                                       gTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAtt              >  2:354929/1‑149 (MQ=255)
                                                                                                                                       gTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAtt              <  2:1033735/149‑1 (MQ=255)
                                                                                                                                         gCGGGTGGGTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCTTCGGCAATGGAAATATAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCTACCACGCGTGTCTTCCGCGTTTTGGTATTGc            <  1:634312/149‑1 (MQ=255)
                                                                                                                                            ggtggaAGGTCATCAACCACTCGCCCCCGCTTTCAGGGAACTGTTTCGCTTCTTCGTCGTCAATGCAAATAAAGGTGCCATCGCAAGCCGGATTCAAACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTAATGCTTc         >  2:685875/1‑149 (MQ=255)
                                                                                                                                             gtggtTGATGACCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGATATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCTACCACGCGTGTATTACGCGTTTTGGTATTGCTTcc        >  2:677094/1‑148 (MQ=255)
                                                                                                                                              tggttggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCAc       <  2:624734/149‑1 (MQ=255)
                                                                                                                                               ggttggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCAcc      <  2:45090/149‑1 (MQ=255)
                                                                                                                                                   ggtCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGcac  >  2:709161/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGTTTTGGTATTGCTTCACCGCAC  >  NC_000913/2399728‑2400023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑